FastQCFastQC Report
Thu 26 May 2016
SRR1364569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793256
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27050.15084293597790835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30400.065.76891
GTATCAA57300.056.3897361
ATCAACG71800.044.4179883
TCAACGC72900.043.5845154
CAACGCA73600.042.927465
AACGCAG76000.041.333536
TATCAAC77400.041.281152
GTACATG113400.038.620651
TACATGG114800.036.7470782
ACGCAGA89200.034.9510887
ACATGGG118500.034.143583
CGCAGAG89000.034.0946778
GCAGAGT96300.030.336239
CATGGGA77150.029.8465084
CATGGGG44300.027.6682684
ATGGGAG30350.027.2505075
TACCGTA900.00953278626.4430627
TGCACCG5050.024.7426625
CAGAGTA96800.024.52710310-11
AGAGTAC93200.023.87835910-11
Produced by FastQC (version 0.11.5)