FastQCFastQC Report
Thu 26 May 2016
SRR1364569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793256
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70160.39124363727209055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62630.34925297893886875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40970.22846710118354546No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18520.1032758289948563No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42400.069.0306551
GTATCAA80400.055.607271
ATCAACG105450.042.516093
TCAACGC107350.041.7081344
CAACGCA107650.041.4523625
AACGCAG110800.040.649786
TATCAAC111350.039.942512
GTACATG103350.034.9533271
ACGCAGA131000.033.9728857
CGCAGAG130950.033.849558
TACATGG104600.033.5265272
ACATGGG106150.032.0834433
GCAGAGT140000.030.8114479
TGGTATC20050.028.8046322
GTGGTAT20650.028.2899631
CATGGGA70550.027.596614
GAGTACT86900.027.35263312-13
AGTACTT90100.025.58874712-13
GTACTTT93600.025.55361414-15
GTACCGT2109.8210876E-825.4991266
Produced by FastQC (version 0.11.5)