##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364569_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1793256 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.74289170090606 31.0 30.0 33.0 26.0 34.0 2 29.64551742751732 31.0 30.0 34.0 25.0 34.0 3 29.91658525051638 31.0 30.0 34.0 26.0 34.0 4 33.466238506939334 35.0 33.0 37.0 28.0 37.0 5 32.895734351369796 35.0 33.0 37.0 26.0 37.0 6 32.928892472686556 35.0 33.0 37.0 26.0 37.0 7 32.7081125059668 35.0 33.0 37.0 25.0 37.0 8 32.62798618825198 35.0 33.0 37.0 25.0 37.0 9 33.82704811806011 38.0 33.0 39.0 23.0 39.0 10-11 33.749284541638225 37.5 33.0 39.0 23.0 39.0 12-13 33.63237122864778 37.5 33.0 39.0 22.5 39.0 14-15 34.53652936334801 38.0 33.0 41.0 21.5 41.0 16-17 34.54858759708597 38.0 33.0 41.0 22.0 41.0 18-19 34.568421630821256 38.5 33.5 41.0 22.0 41.0 20-21 34.595038020226895 39.0 34.0 41.0 22.0 41.0 22-23 34.54338086698162 39.0 33.5 41.0 22.0 41.0 24-25 34.4969544225699 39.0 33.0 41.0 22.0 41.0 26-27 34.35801023389856 39.0 33.0 41.0 21.5 41.0 28-29 34.19318797762283 38.0 33.0 40.5 20.0 41.0 30-31 33.99940722350853 38.0 33.0 40.0 18.5 41.0 32-33 33.755600148556596 38.0 33.0 40.0 18.0 41.0 34-35 33.55135128503683 38.0 32.5 40.0 16.0 41.0 36-37 33.32121459512753 38.0 32.0 40.0 15.5 41.0 38-39 33.136705244538426 38.0 32.0 40.0 14.5 41.0 40-41 33.14211663030822 38.0 32.0 40.0 11.5 41.0 42-43 32.97795741377695 38.0 32.0 40.0 9.0 41.0 44-45 32.777858264519956 38.0 31.5 40.0 7.5 41.0 46-47 32.48841799497673 38.0 31.0 40.0 2.0 41.0 48-49 32.1611593659801 38.0 30.5 40.0 2.0 41.0 50-51 31.97200343955353 37.5 30.0 40.0 2.0 41.0 52-53 31.782800113313435 37.0 30.0 40.0 2.0 41.0 54-55 31.58620213734124 37.0 30.0 40.0 2.0 41.0 56-57 31.362342298032182 37.0 30.0 40.0 2.0 41.0 58-59 31.1510659939239 36.0 29.5 40.0 2.0 41.0 60-61 30.904710203116565 36.0 29.0 40.0 2.0 41.0 62-63 30.731059870983284 35.5 29.0 39.0 2.0 41.0 64-65 30.59477146598143 35.0 29.0 39.0 2.0 41.0 66-67 30.294625530320268 35.0 29.0 39.0 2.0 41.0 68-69 30.010352119273545 35.0 29.0 38.5 2.0 40.5 70-71 29.70712770513524 35.0 29.0 37.5 2.0 40.0 72-73 29.428536416440263 35.0 29.0 37.0 2.0 39.5 74-75 29.08927336643513 35.0 28.0 37.0 2.0 39.0 76-77 28.784381036505664 34.0 28.0 36.0 2.0 39.0 78-79 28.50871515277239 34.0 28.0 36.0 2.0 38.5 80-81 28.235106420946032 34.0 27.5 35.0 2.0 37.0 82-83 27.950461897241667 34.0 27.0 35.0 2.0 37.0 84-85 27.740020387496266 34.0 27.0 35.0 2.0 36.5 86-87 27.53128080987879 34.0 26.0 35.0 2.0 36.0 88-89 27.360884056710255 34.0 26.0 35.0 2.0 36.0 90-91 27.217880492244277 34.0 26.0 35.0 2.0 35.5 92-93 27.103471283520033 34.0 26.0 35.0 2.0 35.0 94-95 26.9887060185495 34.0 26.0 35.0 2.0 35.0 96-97 26.879308921871726 34.0 25.0 35.0 2.0 35.0 98-99 26.794467159178613 34.0 25.0 35.0 2.0 35.0 100-101 26.68895154958355 34.0 25.0 35.0 2.0 35.0 102-103 25.436878225975544 31.5 23.5 34.0 2.0 35.0 104-105 26.357006194319162 33.0 24.5 35.0 2.0 35.0 106-107 26.514223011103823 33.5 25.0 35.0 2.0 35.0 108-109 26.521047747783918 34.0 25.0 35.0 2.0 35.0 110-111 26.43993746570484 34.0 25.0 35.0 2.0 35.0 112-113 26.349250469536976 34.0 25.0 35.0 2.0 35.0 114-115 26.245508449434993 34.0 24.0 35.0 2.0 35.0 116-117 26.116363196331143 33.5 24.0 35.0 2.0 35.0 118-119 25.97435474912673 33.0 24.0 35.0 2.0 35.0 120-121 25.771311234982623 33.0 23.0 35.0 2.0 35.0 122-123 25.466250775126362 33.0 18.5 35.0 2.0 35.0 124-125 23.686599124720622 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0410795794373016 1101 2 1.2977999536549163 1101 3 1.0221342688771529 1101 4 0.9993981657644895 1101 5 1.1777294344787563 1101 6 1.2438634353498301 1101 7 1.367207327460335 1101 8 1.4215069533444122 1101 9 1.7038557022963374 1101 10-11 1.5588097153855571 1101 12-13 1.632004090600077 1101 14-15 1.8150782800019343 1101 16-17 1.7728235978090794 1101 18-19 1.8926920750648364 1101 20-21 1.8696853751916152 1101 22-23 1.8026436682154028 1101 24-25 1.9426185245010785 1101 26-27 1.8443585679041945 1101 28-29 1.907903645075784 1101 30-31 1.9096382267879548 1101 32-33 2.035705128725347 1101 34-35 1.980970388164316 1101 36-37 2.04921963956982 1101 38-39 1.9970190056662034 1101 40-41 1.8677713683505672 1101 42-43 1.9340064520093634 1101 44-45 1.84337079577476 1101 46-47 1.9412943361154404 1101 48-49 1.8421663373848034 1101 50-51 1.9376266733393557 1101 52-53 2.024667361215066 1101 54-55 2.067274085207199 1101 56-57 2.0198221316065705 1101 58-59 2.0290020067362065 1101 60-61 2.001824901458999 1101 62-63 1.9556680814250207 1101 64-65 1.9064278430834811 1101 66-67 1.9829326466565078 1101 68-69 2.0106957326127137 1101 70-71 2.038647349237472 1101 72-73 1.885206173134609 1101 74-75 1.9143164123799288 1101 76-77 1.9883006201837894 1101 78-79 1.9737731627017645 1101 80-81 1.821812001164819 1101 82-83 1.9798342311197956 1101 84-85 1.9253881552730228 1101 86-87 2.0214351564609174 1101 88-89 1.8941478912754768 1101 90-91 1.9416133214855478 1101 92-93 1.9329005810099211 1101 94-95 1.8907205469985549 1101 96-97 2.039183897353361 1101 98-99 1.966412546807618 1101 100-101 1.948018916471618 1101 102-103 1.8121224275319499 1101 104-105 1.9461388948892022 1101 106-107 1.8137611836326073 1101 108-109 1.8230153132727054 1101 110-111 1.8041260251115006 1101 112-113 1.8121607587757467 1101 114-115 1.8038658292276821 1101 116-117 1.819929654921495 1101 118-119 1.8499038268066208 1101 120-121 1.7221069942758227 1101 122-123 1.7032685185954186 1101 124-125 1.9066638849507491 1102 1 0.502120461879823 1102 2 -0.24042177191459402 1102 3 0.3686362453116807 1102 4 0.3685623910169724 1102 5 0.6991403569124159 1102 6 0.41581305857901896 1102 7 0.5092071301962378 1102 8 0.6881382858923004 1102 9 1.180484422720724 1102 10-11 1.177317748478231 1102 12-13 1.223913263850342 1102 14-15 1.3925839224416094 1102 16-17 1.3103828989739128 1102 18-19 1.4446120942276366 1102 20-21 1.1702952680945558 1102 22-23 1.4001176038834586 1102 24-25 1.3514873883140623 1102 26-27 1.4572536064230803 1102 28-29 1.0380238858535975 1102 30-31 1.3566354228197 1102 32-33 1.4424145648498907 1102 34-35 1.507807699115041 1102 36-37 1.1974961257976489 1102 38-39 0.7013824441745413 1102 40-41 0.2253557213333579 1102 42-43 0.6730807848069063 1102 44-45 0.3922052214670728 1102 46-47 0.536838804898764 1102 48-49 0.08372543911779928 1102 50-51 0.029726788121049452 1102 52-53 -0.11038466267484637 1102 54-55 0.02574475373378604 1102 56-57 0.11429989296975762 1102 58-59 0.18498299278934738 1102 60-61 0.05771350577213852 1102 62-63 -0.03580945919025069 1102 64-65 0.04159367318418106 1102 66-67 -0.028764102785210355 1102 68-69 0.1710801157993096 1102 70-71 -0.30660257823364745 1102 72-73 -0.12068718213622631 1102 74-75 0.006734823262259937 1102 76-77 -0.44718751633163834 1102 78-79 -0.23911580615254735 1102 80-81 -0.16464433717273508 1102 82-83 -0.4944293072128474 1102 84-85 -0.3463232697505454 1102 86-87 -0.059604078501216406 1102 88-89 -0.26379616159130137 1102 90-91 -0.17034362869587483 1102 92-93 0.046620082300268706 1102 94-95 0.304339465275941 1102 96-97 0.02331768576701876 1102 98-99 -0.06221405067932295 1102 100-101 0.014682801208689256 1102 102-103 0.003055649299067653 1102 104-105 0.09134279407062706 1102 106-107 0.16046764995698481 1102 108-109 0.2502739962349736 1102 110-111 0.30755538559646567 1102 112-113 0.389845781480993 1102 114-115 0.3003159786751084 1102 116-117 0.2428053536103434 1102 118-119 0.4474659171268449 1102 120-121 0.7939501454722517 1102 122-123 0.5323286996809671 1102 124-125 0.7083720981101465 1103 1 0.6140919856110827 1103 2 0.17187191282988223 1103 3 0.6174887135609559 1103 4 0.5257907896071998 1103 5 0.6276647907169064 1103 6 0.719672444384841 1103 7 0.8534589753351298 1103 8 0.8907409548293472 1103 9 1.0186819887209424 1103 10-11 1.118870521407196 1103 12-13 0.9811496350936366 1103 14-15 1.1440856521050335 1103 16-17 1.0779583018031786 1103 18-19 1.218195147146666 1103 20-21 1.2672068890409847 1103 22-23 1.280869388496022 1103 24-25 1.1931442811663544 1103 26-27 1.1082207294692736 1103 28-29 1.2409595410575562 1103 30-31 1.245284339727462 1103 32-33 1.3257929363587024 1103 34-35 1.2683695444111933 1103 36-37 1.3803491067835978 1103 38-39 1.3208723991802458 1103 40-41 1.2170552020503251 1103 42-43 1.1905767353940107 1103 44-45 1.115886597678653 1103 46-47 1.1668888680399832 1103 48-49 1.1897778165227137 1103 50-51 1.1307990002433534 1103 52-53 0.9205338710737934 1103 54-55 0.9723819215840983 1103 56-57 1.1267255287796978 1103 58-59 1.219615210280402 1103 60-61 1.27210728973591 1103 62-63 1.1403669734278488 1103 64-65 1.1638005474397168 1103 66-67 1.0532309768444037 1103 68-69 1.1578495346038729 1103 70-71 0.9763536449523613 1103 72-73 0.8707723875526234 1103 74-75 0.9946109048276206 1103 76-77 0.9843974288124855 1103 78-79 0.863483827324842 1103 80-81 0.8394032471316706 1103 82-83 0.9724717481396752 1103 84-85 1.0349291828459997 1103 86-87 1.1308432202505507 1103 88-89 1.1705430060417754 1103 90-91 1.0576367629661974 1103 92-93 1.0325000748542479 1103 94-95 1.05941224864981 1103 96-97 1.1075315766886895 1103 98-99 1.1589250366420742 1103 100-101 1.0453762355094547 1103 102-103 0.7710108503031847 1103 104-105 0.866707808094084 1103 106-107 1.081711552065972 1103 108-109 1.0894323035017557 1103 110-111 0.895380924929988 1103 112-113 0.9088903650689559 1103 114-115 1.0087065011654843 1103 116-117 1.08442348035139 1103 118-119 0.9196342264160435 1103 120-121 0.9336260900396383 1103 122-123 0.9884619190565651 1103 124-125 1.3552965740453509 1104 1 0.637004097137627 1104 2 0.802207136796806 1104 3 0.5863990450480756 1104 4 0.39838367782992634 1104 5 0.7163935966502422 1104 6 0.7737572272917035 1104 7 0.7750743874449384 1104 8 0.7160677743266177 1104 9 0.7302731273577123 1104 10-11 0.8036968988712729 1104 12-13 0.8703574076424303 1104 14-15 0.9649671814826704 1104 16-17 0.8901068991463106 1104 18-19 0.7219297037476338 1104 20-21 0.5731662773980091 1104 22-23 0.7958388697727443 1104 24-25 0.9746199988778415 1104 26-27 0.847520572233222 1104 28-29 1.0610673119806293 1104 30-31 0.9895689912493708 1104 32-33 1.135850949066274 1104 34-35 1.1622396166454365 1104 36-37 1.2722355249728778 1104 38-39 1.3241863315950724 1104 40-41 1.3203992673682023 1104 42-43 1.2810834627477945 1104 44-45 1.2848648655816888 1104 46-47 1.4327894125158949 1104 48-49 1.6355849009147647 1104 50-51 1.4296282133143485 1104 52-53 1.403219816387157 1104 54-55 1.1519962914068707 1104 56-57 1.3854027630279795 1104 58-59 1.395489986086794 1104 60-61 1.4946626492138897 1104 62-63 1.5666020961656208 1104 64-65 1.572063316887057 1104 66-67 1.575844678751686 1104 68-69 1.2240709967869634 1104 70-71 0.9763944594657978 1104 72-73 0.8212527623546464 1104 74-75 1.315830227254036 1104 76-77 1.5415394649220495 1104 78-79 1.4988648814681298 1104 80-81 1.4914659885984598 1104 82-83 1.4039041669760337 1104 84-85 1.471265182116948 1104 86-87 1.4427641607634811 1104 88-89 1.3344053778996603 1104 90-91 1.4330132239025275 1104 92-93 1.4156069649165453 1104 94-95 1.3196794171346404 1104 96-97 1.3667521076368523 1104 98-99 1.4192827068740854 1104 100-101 1.4140491558983719 1104 102-103 1.3162764354367624 1104 104-105 1.3981562779677077 1104 106-107 1.3972395682241725 1104 108-109 1.3318421206000721 1104 110-111 1.4163811655810576 1104 112-113 1.3548446221314663 1104 114-115 1.3536480431046023 1104 116-117 1.3283935796273951 1104 118-119 1.2524873452287544 1104 120-121 1.2312255440753965 1104 122-123 1.2422534900409588 1104 124-125 1.598837405388334 1105 1 0.6690953887245961 1105 2 0.34578048079213985 1105 3 0.677765995876161 1105 4 0.6426413483409448 1105 5 0.4376137250793306 1105 6 0.8294971315376642 1105 7 0.9718652922849742 1105 8 0.9182567714750292 1105 9 1.0539087751661853 1105 10-11 1.1116351612503763 1105 12-13 1.1312704912391993 1105 14-15 1.2216783615583253 1105 16-17 1.2262097693420841 1105 18-19 1.320930616953632 1105 20-21 1.313934374527399 1105 22-23 1.3024498102479853 1105 24-25 1.1320238804906282 1105 26-27 1.220522098323059 1105 28-29 1.3396352014457662 1105 30-31 1.3756697195249146 1105 32-33 1.5293006736651975 1105 34-35 1.527122018424798 1105 36-37 1.6678595252684474 1105 38-39 1.68796145127628 1105 40-41 1.401242136779743 1105 42-43 1.4035911572279147 1105 44-45 1.4796969900333679 1105 46-47 1.4875571796309188 1105 48-49 1.4440332576312969 1105 50-51 1.2107053793340263 1105 52-53 1.3243336874399319 1105 54-55 1.2882774396302459 1105 56-57 1.2889758939645866 1105 58-59 1.0488498316328076 1105 60-61 1.0937707150961877 1105 62-63 1.2138391097258996 1105 64-65 1.126313453278648 1105 66-67 1.342075787638521 1105 68-69 1.3216265671508545 1105 70-71 1.3747917931490896 1105 72-73 1.3886388195645445 1105 74-75 1.2924382483713295 1105 76-77 1.2790003352125758 1105 78-79 1.3046674567135597 1105 80-81 1.2962623713145014 1105 82-83 1.2681375895900437 1105 84-85 1.2263218704725425 1105 86-87 1.2856094898700938 1105 88-89 1.2578301995592973 1105 90-91 1.1391057856821938 1105 92-93 1.199866299098943 1105 94-95 1.2262978713300754 1105 96-97 1.2401858709635079 1105 98-99 1.0206710111736328 1105 100-101 1.0336799908460783 1105 102-103 0.7515817746659295 1105 104-105 1.0223781319458638 1105 106-107 0.9935426818962156 1105 108-109 0.97249102950137 1105 110-111 0.9075703370296466 1105 112-113 0.8897978138079452 1105 114-115 0.8932443852034879 1105 116-117 0.7742504259184386 1105 118-119 0.8968535056996032 1105 120-121 0.9210583247026456 1105 122-123 0.9292045379839351 1105 124-125 1.2662377647322849 1106 1 0.005531449090796059 1106 2 -0.4066817119108386 1106 3 0.17143175528620347 1106 4 -0.12882304331787253 1106 5 0.15770884935048457 1106 6 0.4054980851504695 1106 7 0.5406792956149502 1106 8 0.4629686991984272 1106 9 0.5743450530230234 1106 10-11 0.634575718340102 1106 12-13 0.6067993089231365 1106 14-15 0.6103520834261289 1106 16-17 0.6333410922114737 1106 18-19 0.6824214943715106 1106 20-21 0.6818333835856762 1106 22-23 0.6075722724650916 1106 24-25 0.5803600945292899 1106 26-27 0.7157568226554147 1106 28-29 0.8130640494180668 1106 30-31 0.8238351476451342 1106 32-33 0.7039146364945097 1106 34-35 0.7288789175659574 1106 36-37 0.7209033660137081 1106 38-39 0.7713433191793015 1106 40-41 0.6630809992200497 1106 42-43 0.6367275290867198 1106 44-45 0.7314293343419607 1106 46-47 0.7734702342625397 1106 48-49 0.8795223623610511 1106 50-51 0.891675018128776 1106 52-53 0.8370837276094747 1106 54-55 0.7772373499014087 1106 56-57 0.3386606545279598 1106 58-59 0.8018947877146871 1106 60-61 0.7693666969566308 1106 62-63 0.6498843143187187 1106 64-65 0.8294913609699037 1106 66-67 0.6633643569370022 1106 68-69 0.7329919866272121 1106 70-71 0.7918658190855474 1106 72-73 0.9078590319734303 1106 74-75 0.7268465222171407 1106 76-77 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22-23 -4.903541021933176 2216 24-25 -4.738039158905227 2216 26-27 -4.504710821622599 2216 28-29 -4.447874516047506 2216 30-31 -4.648943351079133 2216 32-33 -4.743426555089531 2216 34-35 -4.839671319079947 2216 36-37 -4.600216850262317 2216 38-39 -4.643117172095927 2216 40-41 -4.795559593853085 2216 42-43 -4.804141568271135 2216 44-45 -4.672783394077037 2216 46-47 -4.837710102222246 2216 48-49 -4.83154237203712 2216 50-51 -4.737566116109349 2216 52-53 -4.586278144123263 2216 54-55 -4.531743459993908 2216 56-57 -4.374551088839368 2216 58-59 -4.72920320256619 2216 60-61 -4.552444618750254 2216 62-63 -4.927949806778919 2216 64-65 -4.6346030306469075 2216 66-67 -4.442665532593946 2216 68-69 -4.229006845083301 2216 70-71 -4.236062216339903 2216 72-73 -4.088930942266419 2216 74-75 -4.123092985557808 2216 76-77 -4.126562269435492 2216 78-79 -4.147714698002478 2216 80-81 -4.115461288522738 2216 82-83 -3.99203187110988 2216 84-85 -4.023245606755925 2216 86-87 -4.151329928306968 2216 88-89 -4.014013409648296 2216 90-91 -4.042941413957781 2216 92-93 -4.17741815829055 2216 94-95 -4.198458451280256 2216 96-97 -4.214118306213919 2216 98-99 -4.221554607253637 2216 100-101 -4.221849257986371 2216 102-103 -3.8065905549437034 2216 104-105 -3.858641441571784 2216 106-107 -4.019686072055112 2216 108-109 -3.989779557363633 2216 110-111 -4.076985185118495 2216 112-113 -3.9541237659730157 2216 114-115 -4.243428263840315 2216 116-117 -4.272709590055204 2216 118-119 -4.309307104487388 2216 120-121 -4.467879288700356 2216 122-123 -4.63183283175135 2216 124-125 -4.905681282018872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65048.0 3 21003.0 4 10301.0 5 8044.0 6 9147.0 7 10639.0 8 11712.0 9 13048.0 10 14127.0 11 15169.0 12 16930.0 13 19495.0 14 22448.0 15 19497.0 16 12893.0 17 11090.0 18 10186.0 19 9657.0 20 9526.0 21 9953.0 22 11284.0 23 13820.0 24 18112.0 25 23942.0 26 29688.0 27 32579.0 28 37264.0 29 43551.0 30 50504.0 31 59061.0 32 71254.0 33 89005.0 34 113834.0 35 157219.0 36 247107.0 37 354641.0 38 120120.0 39 358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.708824074466875 19.946603643411027 14.325696564011995 23.018875718110106 2 15.910350680940905 21.42061303083668 38.595703676110574 24.073332612111837 3 19.503740681754305 25.882640292295132 29.52991653171661 25.08370249423395 4 12.98280279308093 17.610196643167725 36.397126542641374 33.00987402110997 5 12.964183585611872 39.24481501804539 33.48478967866272 14.30621171768002 6 31.174299709578555 37.60868498418519 16.925302355045794 14.29171295119046 7 27.82697823091392 33.612234006661716 20.568013825324233 17.99277393710013 8 26.556268825101736 35.42557207954043 19.16994162789414 18.848217467463698 9 26.928445515903753 15.68492627397051 19.62861777845441 37.75801043167132 10-11 25.1489960281667 27.30011529330086 26.5010644067135 21.04982427181894 12-13 26.116572313155512 24.62308783575797 27.560259104110063 21.70008074697645 14-15 22.947845412993296 25.51465755995172 26.067952949259045 25.46954407779594 16-17 22.035082592873067 29.02279877808721 26.49022391696882 22.451894712070906 18-19 21.89798850614774 26.631318698017726 29.872751216457328 21.597941579377203 20-21 22.894358886850675 25.758788648346158 29.94041778649944 21.406434678303725 22-23 22.457000004461158 25.8392834040427 29.803664395936774 21.900052195559365 24-25 22.09720753757411 26.019848811324202 29.98793814157042 21.895005509531266 26-27 21.87451206074314 26.39915884848566 29.798227358503194 21.92810173226801 28-29 21.772853401856736 26.348803517177693 30.036843596229428 21.841499484736147 30-31 21.973159437358635 25.984438362397782 30.02605316809201 22.016349032151574 32-33 21.801990346052097 26.063149935090134 29.812280009100768 22.322579709757 34-35 22.202686063785652 26.100818845719743 29.7487084944927 21.947786596001908 36-37 22.395324482394035 25.861394022939276 29.505603215603347 22.237678279063335 38-39 22.11795192655148 26.001502295266267 29.61693701289721 22.263608765285046 40-41 22.283321511262198 25.975376633341806 29.61852630076241 22.122775554633584 42-43 22.289622898236505 26.02729337027173 29.300863903424833 22.382219828066933 44-45 22.28948348702026 26.210758530851148 29.08407946216268 22.41567851996592 46-47 22.545330951074472 26.547743322760386 28.39218159593499 22.514744130230152 48-49 22.70476161797312 26.67706116694995 27.990677293147215 22.62749992192972 50-51 22.64447758097849 26.84575079715422 27.800161048016626 22.709610573850664 52-53 22.97959688968 26.67084342670539 27.76516570974808 22.584393973866533 54-55 22.61146763206146 26.644104355429455 27.867660835931957 22.876767176577133 56-57 22.583779020807423 26.92411508496411 27.654940299256598 22.837165594971864 58-59 22.789043664127416 26.48343464405738 27.739202468373687 22.98831922344152 60-61 22.741956530467487 26.61884304304572 28.046190839456276 22.59300958703052 62-63 22.60946011054752 26.618285398180742 28.10530119514447 22.666953296127268 64-65 22.443424758406998 26.513931242849935 28.037363373332326 23.00528062541074 66-67 22.5213522218802 26.880350602479513 27.752702347015706 22.84559482862458 68-69 22.625638120167483 26.553801173340887 28.001252470715972 22.819308235775658 70-71 22.35180030068211 26.623359966452085 28.03057678323675 22.994262949629054 72-73 22.595128637517455 26.725855092635964 27.691110471678332 22.98790579816825 74-75 22.548408377494724 26.902109207225163 27.545472339623462 23.00401007565665 76-77 22.682818292536037 26.65829641724327 27.74233015252702 22.91655513769367 78-79 22.6362828285532 26.792493654001436 27.589479695035173 22.981743822410188 80-81 22.455094912629523 27.016745252835 27.639161089516296 22.888998745019183 82-83 22.74602733798186 26.969350722930802 27.390707182911978 22.89391475617536 84-85 22.593065351517016 26.858295748069434 27.408523936348182 23.140114964065365 86-87 22.507968745120607 26.7541276872906 27.536726490807784 23.201177076781008 88-89 22.516592852498434 26.810770660067075 27.41473264623514 23.257903841199347 90-91 22.520097520933987 27.03752280767498 27.470952278983034 22.971427392408 92-93 22.490079320821046 26.801950007361043 27.551772673129516 23.156197998688395 94-95 22.380268071039495 26.602978046636956 27.57255517338294 23.444198708940608 96-97 22.486118380454947 26.56188959006106 27.479848542349778 23.472143487134215 98-99 22.245359171865744 26.58486633439058 27.393649576522254 23.776124917221427 100-101 22.24107413719262 26.631454325636177 27.54966495949177 23.577806577679432 102-103 22.448914153918906 26.36533768742444 27.52041537850703 23.66533278014963 104-105 22.32054430599981 26.30522357097927 27.65466837975169 23.719563743269227 106-107 22.227485852328872 26.52306343643976 27.570699319885705 23.678751391345664 108-109 22.34488550435632 26.415414196299913 27.25388901528839 23.985811284055373 110-111 22.141450281388224 26.474946075621276 27.529845219746026 23.853758423244475 112-113 22.155927541856823 26.5144519243209 27.536419786132043 23.793200747690236 114-115 22.083824060814518 26.599883117636296 27.376989119233397 23.939303702315787 116-117 21.879084748636004 26.503549967210482 27.550807023648606 24.066558260504912 118-119 21.877328167311305 26.66989543043492 27.24111894788028 24.211657454373498 120-121 21.783467159777008 26.735779813444132 27.434318717541352 24.04643430923751 122-123 21.878281547047102 26.639567559368295 27.273054270897756 24.20909662268685 124-125 21.82086639167927 26.55570615584982 27.220459017418193 24.402968435052717 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 385.0 1 327.0 2 490.0 3 721.5 4 488.5 5 313.5 6 717.5 7 1744.5 8 2861.5 9 3274.5 10 2558.0 11 1563.5 12 1272.0 13 1298.0 14 1440.0 15 1651.5 16 1889.5 17 2153.5 18 2439.0 19 2732.0 20 3023.0 21 3482.0 22 4162.5 23 4917.0 24 5848.0 25 7342.5 26 9701.5 27 12398.0 28 14818.0 29 17541.5 30 21149.5 31 25138.0 32 29386.5 33 33196.0 34 36850.5 35 39493.0 36 42403.5 37 46931.0 38 49588.0 39 50180.0 40 50968.0 41 52896.0 42 54532.5 43 56543.5 44 59199.5 45 61147.5 46 61585.5 47 61768.0 48 64456.5 49 65838.5 50 64399.0 51 62202.0 52 60700.0 53 61143.0 54 60450.0 55 56423.0 56 51840.0 57 48144.0 58 45800.0 59 42435.0 60 36462.5 61 29598.0 62 23604.0 63 18130.5 64 13322.0 65 10411.0 66 7852.5 67 5980.0 68 4317.5 69 2882.5 70 2159.5 71 1818.5 72 1566.0 73 1421.0 74 1144.0 75 864.0 76 622.0 77 342.0 78 202.0 79 142.5 80 101.0 81 58.5 82 36.5 83 21.0 84 16.0 85 11.0 86 4.5 87 3.0 88 5.0 89 3.5 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09747632239903282 2 1.1152897299660506E-4 3 0.0 4 0.09401892423613807 5 0.0 6 0.0 7 0.0013941121624575633 8 0.005855271082321765 9 0.003513162649393059 10-11 1.9517570274405887E-4 12-13 0.0 14-15 2.7882243249151265E-5 16-17 1.1152897299660506E-4 18-19 0.002035403757188042 20-21 0.0010874074867168993 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.1152897299660506E-4 52-53 0.0 54-55 0.0 56-57 0.008141615028752169 58-59 5.855271082321766E-4 60-61 0.0 62-63 0.0 64-65 1.394112162457563E-4 66-67 0.0 68-69 2.7882243249151265E-5 70-71 0.0 72-73 0.0 74-75 0.001282583189460958 76-77 0.0 78-79 0.0 80-81 8.36467297474538E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.807028109762355E-4 90-91 0.0 92-93 0.001784463567945681 94-95 0.0 96-97 0.008225261758499624 98-99 0.003819867325133723 100-101 0.002230579459932101 102-103 0.0 104-105 0.0 106-107 0.001561405621952471 108-109 0.0 110-111 2.2305794599321012E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 7.249383244779329E-4 122-123 0.004739981352355715 124-125 0.08573789799114014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1793256.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.10736967512266 #Duplication Level Percentage of deduplicated Percentage of total 1 76.0603213867497 39.63303284108006 2 12.310033578236345 12.828869407486682 3 4.5344774007264 7.088390706094196 4 2.229257936112287 4.646430695128156 5 1.28925796250974 3.358992062954523 6 0.7763648771121712 2.4272598992679044 7 0.5073787127358556 1.8506719104870561 8 0.36133988148667406 1.5062776626392909 9 0.27917562407590985 1.3092396703205855 >10 1.473717447760981 14.154387898072907 >50 0.11991553627024891 4.281642690429736 >100 0.0562802086322453 5.096939667482578 >500 0.0019404372454272054 0.663906591615085 >1k 3.234062075712009E-4 0.4080393841688787 >5k 2.156041383808006E-4 0.7459189127723762 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7016 0.39124363727209055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6263 0.34925297893886875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4097 0.22846710118354546 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1852 0.1032758289948563 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.576448649830253E-5 0.0 6 0.0 0.0 0.0 5.576448649830253E-5 0.0 7 0.0 0.0 0.0 5.576448649830253E-5 0.0 8 0.0 0.0 0.0 5.576448649830253E-5 0.0 9 0.0 0.0 0.0 5.576448649830253E-5 0.0 10-11 0.0 0.0 0.0 5.576448649830253E-5 0.0 12-13 0.0 0.0 0.0 5.576448649830253E-5 0.0 14-15 0.0 0.0 0.0 8.36467297474538E-5 0.0 16-17 0.0 0.0 0.0 1.1152897299660506E-4 0.0 18-19 0.0 0.0 0.0 1.1152897299660506E-4 0.0 20-21 0.0 0.0 0.0 1.672934594949076E-4 0.0 22-23 0.0 0.0 0.0 5.29762621733874E-4 0.0 24-25 0.0 0.0 0.0 0.0013104654327101095 0.0 26-27 0.0 0.0 0.0 0.0016171701084507733 0.0 28-29 0.0 0.0 0.0 0.0021469327301846474 0.0 30-31 0.0 0.0 0.0 0.004572687892860808 0.0 32-33 0.0 0.0 0.0 0.009229022515469068 0.0 34-35 0.0 0.0 0.0 0.014693942192302717 0.0 36-37 0.0 0.0 0.0 0.023727789005027727 0.0 38-39 0.0 0.0 0.0 0.03886784708931686 0.0 40-41 0.0 0.0 0.0 0.05919400241794813 0.0 42-43 0.0 0.0 0.0 0.0794086287735828 0.0 44-45 0.0 0.0 0.0 0.10302488880561392 0.0 46-47 0.0 0.0 0.0 0.13062830962227368 0.0 48-49 0.0 0.0 0.0 0.1628323005750434 0.0 50-51 0.0 0.0 0.0 0.20197897009685178 0.0 52-53 0.0 0.0 0.0 0.24056799475367713 0.0 54-55 0.0 0.0 0.0 0.28194524373541757 0.0 56-57 0.0 0.0 0.0 0.330125760069951 0.0 58-59 0.0 0.0 0.0 0.38379907832456717 0.0 60-61 0.0 0.0 0.0 0.4506606976360319 0.0 62-63 0.0 0.0 0.0 0.520728774921149 0.0 64-65 0.0 0.0 0.0 0.5870606316108798 0.0 66-67 2.7882243249151265E-5 0.0 0.0 0.6671663164656915 0.0 68-69 5.576448649830253E-5 0.0 0.0 0.7650887547567107 0.0 70-71 5.576448649830253E-5 0.0 0.0 0.8765340810235682 0.0 72-73 5.576448649830253E-5 0.0 0.0 0.9894292839393817 0.0 74-75 5.576448649830253E-5 0.0 0.0 1.1036349522879054 0.0 76-77 5.576448649830253E-5 0.0 0.0 1.2308616282337825 0.0 78-79 5.576448649830253E-5 0.0 0.0 1.3805056277519774 0.0 80-81 5.576448649830253E-5 0.0 0.0 1.5549090592754187 0.0 82-83 5.576448649830253E-5 0.0 0.0 1.7388761002333186 0.0 84-85 5.576448649830253E-5 0.0 0.0 1.9221182028667407 0.0 86-87 5.576448649830253E-5 0.0 0.0 2.1170708476648064 0.0 88-89 5.576448649830253E-5 0.0 0.0 2.3347475207109305 0.0 90-91 5.576448649830253E-5 0.0 0.0 2.5970079007124474 0.0 92-93 5.576448649830253E-5 0.0 0.0 2.876611036014936 0.0 94-95 8.36467297474538E-5 0.0 0.0 3.1518924236138064 5.576448649830253E-5 96-97 1.1152897299660506E-4 0.0 0.0 3.434255900997961 5.576448649830253E-5 98-99 1.1152897299660506E-4 0.0 0.0 3.7463697319289606 5.576448649830253E-5 100-101 1.1152897299660506E-4 0.0 0.0 4.106245845545756 5.576448649830253E-5 102-103 1.1152897299660506E-4 0.0 0.0 4.506690623090066 5.576448649830253E-5 104-105 1.1152897299660506E-4 0.0 0.0 4.901475305254799 5.576448649830253E-5 106-107 1.1152897299660506E-4 0.0 0.0 5.295869636014044 5.576448649830253E-5 108-109 1.1152897299660506E-4 0.0 0.0 5.711928469777879 5.576448649830253E-5 110-111 1.1152897299660506E-4 0.0 0.0 6.186121780716194 5.576448649830253E-5 112-113 1.1152897299660506E-4 0.0 0.0 6.713124060368402 5.576448649830253E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4240 0.0 69.030655 1 GTATCAA 8040 0.0 55.60727 1 ATCAACG 10545 0.0 42.51609 3 TCAACGC 10735 0.0 41.708134 4 CAACGCA 10765 0.0 41.452362 5 AACGCAG 11080 0.0 40.64978 6 TATCAAC 11135 0.0 39.94251 2 GTACATG 10335 0.0 34.953327 1 ACGCAGA 13100 0.0 33.972885 7 CGCAGAG 13095 0.0 33.84955 8 TACATGG 10460 0.0 33.526527 2 ACATGGG 10615 0.0 32.083443 3 GCAGAGT 14000 0.0 30.811447 9 TGGTATC 2005 0.0 28.804632 2 GTGGTAT 2065 0.0 28.289963 1 CATGGGA 7055 0.0 27.59661 4 GAGTACT 8690 0.0 27.352633 12-13 AGTACTT 9010 0.0 25.588747 12-13 GTACTTT 9360 0.0 25.553614 14-15 GTACCGT 210 9.8210876E-8 25.499126 6 >>END_MODULE