FastQCFastQC Report
Thu 26 May 2016
SRR1364568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529258
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79850.055.9611
GGTATCA39050.055.919781
ATCAACG92050.047.7038043
TCAACGC93450.046.352434
CAACGCA94400.045.885965
AACGCAG99200.043.538926
TATCAAC100100.043.3325162
ACGCAGA118850.035.789837
CGCAGAG119350.034.745128
GTACATG145150.034.720571
TACATGG151600.032.0658872
ACATGGG151300.031.0676653
GCAGAGT138000.028.6698919
CATGGGG71450.025.3990844
AGAGTAC125350.023.95039410-11
CATGGGA92300.023.5939044
CAGAGTA134600.021.99499310-11
GAGTACT56100.021.37653412-13
GAGTACA110550.020.4524481
ATGGGGA48300.019.5871125
Produced by FastQC (version 0.11.5)