##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364568_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3529258 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.00179527821429 31.0 30.0 34.0 23.0 34.0 2 29.01815169080866 31.0 30.0 34.0 22.0 34.0 3 29.247632788535153 31.0 30.0 34.0 23.0 34.0 4 32.64448391134907 35.0 33.0 37.0 25.0 37.0 5 32.79552104153338 35.0 33.0 37.0 26.0 37.0 6 32.80098479623762 36.0 33.0 37.0 26.0 37.0 7 32.5824212908209 35.0 33.0 37.0 25.0 37.0 8 32.57545183718504 35.0 33.0 37.0 25.0 37.0 9 33.7790895423344 38.0 33.0 39.0 23.0 39.0 10-11 33.6874537083999 38.0 33.5 39.0 22.0 39.0 12-13 33.63284052908571 38.0 33.0 39.0 21.5 39.0 14-15 34.42486976015922 39.0 33.0 41.0 18.0 41.0 16-17 34.55793455734889 39.0 34.0 41.0 19.0 41.0 18-19 34.342621451874585 39.0 33.0 41.0 17.5 41.0 20-21 34.041037662874174 39.0 33.0 41.0 18.0 41.0 22-23 33.96754912789034 39.0 33.0 41.0 17.5 41.0 24-25 33.78634361670356 38.5 32.0 41.0 17.0 41.0 26-27 33.71497634913628 38.0 32.0 41.0 16.5 41.0 28-29 33.79178966230295 38.0 32.5 41.0 16.5 41.0 30-31 33.746852879557125 38.0 32.5 40.0 16.5 41.0 32-33 33.75118169314909 38.0 33.0 40.0 16.0 41.0 34-35 33.26415977522754 38.0 32.0 40.0 10.5 41.0 36-37 33.15966670614617 38.0 31.5 40.0 11.0 41.0 38-39 32.97010830605187 38.0 31.0 40.0 9.0 41.0 40-41 33.20533112059249 38.0 32.0 40.0 13.0 41.0 42-43 33.11961891139724 38.0 32.0 40.0 9.0 41.0 44-45 32.98745345338878 38.0 32.0 40.0 9.0 41.0 46-47 32.97501075296847 38.0 32.0 40.0 8.5 41.0 48-49 32.86160674566722 38.0 32.0 40.0 8.0 41.0 50-51 32.719783166886636 38.0 32.0 40.0 7.0 41.0 52-53 32.669118409592045 38.0 31.5 40.0 4.0 41.0 54-55 32.704735669650674 38.0 32.0 40.0 2.0 41.0 56-57 32.58010536492373 38.0 32.0 40.0 2.0 41.0 58-59 32.38431775177672 37.0 31.0 40.0 2.0 41.0 60-61 32.17960418308891 37.0 31.0 40.0 2.0 41.0 62-63 31.900125323793276 36.5 31.0 40.0 2.0 41.0 64-65 31.612396713416814 36.0 31.0 39.5 2.0 41.0 66-67 31.362351236435536 35.5 30.0 39.0 2.0 41.0 68-69 31.323807015525645 35.0 31.0 39.0 2.0 41.0 70-71 31.078949739576988 35.0 30.5 39.0 2.0 40.5 72-73 30.782901250064462 35.0 30.0 38.0 2.0 40.0 74-75 30.46985088650362 35.0 30.0 37.0 2.0 39.5 76-77 30.15082731270992 35.0 30.0 37.0 2.0 39.0 78-79 29.834085946677746 35.0 30.0 36.0 2.0 39.0 80-81 29.51953200927787 35.0 30.0 36.0 2.0 37.5 82-83 29.26530109161756 34.0 29.0 35.5 2.0 37.0 84-85 29.01672405361127 34.0 29.0 35.0 2.0 37.0 86-87 28.81766110610219 34.0 29.0 35.0 2.0 36.0 88-89 28.618937748387903 34.0 29.0 35.0 2.0 36.0 90-91 28.478882955000742 34.0 29.0 35.0 2.0 36.0 92-93 28.354552430000865 34.0 29.0 35.0 2.0 35.5 94-95 28.2284732371507 34.0 29.0 35.0 2.0 35.0 96-97 28.122714746272447 34.0 29.0 35.0 2.0 35.0 98-99 28.039256268598102 34.0 29.0 35.0 2.0 35.0 100-101 27.947207883356782 34.0 29.0 35.0 2.0 35.0 102-103 26.75448947059127 32.5 25.5 34.0 2.0 35.0 104-105 27.653225408853643 34.0 27.5 35.0 2.0 35.0 106-107 27.804125683075593 34.0 28.0 35.0 2.0 35.0 108-109 27.764312923566372 34.0 28.0 35.0 2.0 35.0 110-111 27.682784454976087 34.0 28.0 35.0 2.0 35.0 112-113 27.595937021322896 34.0 28.0 35.0 2.0 35.0 114-115 27.47906117943205 34.0 27.0 35.0 2.0 35.0 116-117 27.373099671375684 34.0 27.0 35.0 2.0 35.0 118-119 27.235953421370724 34.0 27.0 35.0 2.0 35.0 120-121 27.064495001498898 34.0 27.0 35.0 2.0 35.0 122-123 26.837059517892996 34.0 27.0 35.0 2.0 35.0 124-125 25.362874717575195 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8582309637481238 1101 2 0.9063020524710304 1101 3 0.7919001253749087 1101 4 0.912158876019987 1101 5 0.8880179473428029 1101 6 1.02088154221817 1101 7 1.040562088970006 1101 8 0.9954535644206004 1101 9 1.2707859660609202 1101 10-11 1.3924499920331428 1101 12-13 1.1391109779107538 1101 14-15 1.455648476717343 1101 16-17 1.3030349734565334 1101 18-19 1.5600964389533019 1101 20-21 1.9426265332081982 1101 22-23 1.7964359309908033 1101 24-25 1.8908894426226794 1101 26-27 2.0082636184850173 1101 28-29 1.827929883810512 1101 30-31 1.871058455667594 1101 32-33 1.8205006657048841 1101 34-35 2.1476462466327675 1101 36-37 1.9914749117354376 1101 38-39 1.8454676009828788 1101 40-41 1.7510725906606197 1101 42-43 1.7629798673002455 1101 44-45 1.6767955208856264 1101 46-47 1.5549796724738982 1101 48-49 1.562534146782916 1101 50-51 1.5917374155805888 1101 52-53 1.4625687315389655 1101 54-55 1.3406552674463796 1101 56-57 1.3866448434790044 1101 58-59 1.3458851436832475 1101 60-61 1.2119302359987145 1101 62-63 1.161730231765901 1101 64-65 1.297362536676392 1101 66-67 1.2939621885139552 1101 68-69 1.1455085120847919 1101 70-71 1.0410751267741922 1101 72-73 0.9471744083769487 1101 74-75 0.9798237829874701 1101 76-77 1.0764504545093523 1101 78-79 1.0908211769082001 1101 80-81 1.1696346135853446 1101 82-83 1.0077028384171385 1101 84-85 1.0815740020428741 1101 86-87 1.0498113833713063 1101 88-89 1.0420972313932637 1101 90-91 1.0173969168249322 1101 92-93 0.97363764477182 1101 94-95 1.0090786316626215 1101 96-97 0.9619541528125772 1101 98-99 1.0009841662478038 1101 100-101 1.0810810249310734 1101 102-103 0.9885811755765133 1101 104-105 1.0457182681311998 1101 106-107 1.0452542771561042 1101 108-109 1.0618604647945595 1101 110-111 0.9407147535185558 1101 112-113 0.9403077302327247 1101 114-115 1.1747946597063965 1101 116-117 1.274190174601852 1101 118-119 1.1936417563712922 1101 120-121 1.3665622409059743 1101 122-123 1.481787200983515 1101 124-125 1.6444579417125098 1102 1 0.19826555786951872 1102 2 0.42564993966301046 1102 3 0.5759176976481406 1102 4 0.6708961107492755 1102 5 0.8080509775431537 1102 6 0.08795673042291696 1102 7 -0.40512593883867254 1102 8 0.4744532279192697 1102 9 0.74891032690212 1102 10-11 0.6812147456674325 1102 12-13 0.7920396783745502 1102 14-15 1.0600736688083998 1102 16-17 0.8812379530313876 1102 18-19 1.1604086478461895 1102 20-21 1.4925065738889316 1102 22-23 1.270430342673528 1102 24-25 1.4009149749252785 1102 26-27 1.0670882425862587 1102 28-29 1.4907670573214418 1102 30-31 1.4773939914056626 1102 32-33 1.3327721036927187 1102 34-35 1.745756813972129 1102 36-37 1.8006102066390497 1102 38-39 1.7637669918285894 1102 40-41 1.542239876907722 1102 42-43 1.4033521720901376 1102 44-45 1.3948168578408726 1102 46-47 1.147689252570487 1102 48-49 0.9243058932107289 1102 50-51 1.3341178208240052 1102 52-53 1.003396933403124 1102 54-55 1.0001392862162461 1102 56-57 1.0970347858229204 1102 58-59 1.1319750287538355 1102 60-61 1.0452167221475577 1102 62-63 0.86166321328745 1102 64-65 0.9895565159560782 1102 66-67 0.9629405700059586 1102 68-69 0.8525380201752633 1102 70-71 0.8957922625349859 1102 72-73 1.0225239952176572 1102 74-75 0.6701417027752257 1102 76-77 0.8156322185324534 1102 78-79 0.7980120083858431 1102 80-81 0.5538342441148671 1102 82-83 0.6828349909196341 1102 84-85 0.3516547130492853 1102 86-87 0.7894469428030071 1102 88-89 0.9339923632076221 1102 90-91 0.4814524458846243 1102 92-93 0.8108422110455606 1102 94-95 0.7830718457481467 1102 96-97 0.8931597081607059 1102 98-99 0.9645017158606173 1102 100-101 0.9381112257493349 1102 102-103 0.823306598240265 1102 104-105 1.0005200106116376 1102 106-107 1.003705579683949 1102 108-109 0.5539402379179883 1102 110-111 0.7823655167989116 1102 112-113 0.5424071181196624 1102 114-115 0.6541387498107945 1102 116-117 0.5267641866957042 1102 118-119 0.8849988852564685 1102 120-121 1.199301765628956 1102 122-123 1.074864220337858 1102 124-125 1.3202382643451962 1103 1 0.5600816315394361 1103 2 0.5650028670921259 1103 3 0.5337996256812723 1103 4 0.615829066917918 1103 5 0.6841216297610373 1103 6 0.7082717419487139 1103 7 0.8023077372461671 1103 8 0.8731237747557117 1103 9 1.1498167787315126 1103 10-11 1.0985031581344344 1103 12-13 1.0891947837821832 1103 14-15 1.2598299444546797 1103 16-17 1.2444862434386863 1103 18-19 1.278726814151824 1103 20-21 1.6878604132912187 1103 22-23 1.5712303659012363 1103 24-25 1.6952600273042435 1103 26-27 1.6847484887904614 1103 28-29 1.5653365712387952 1103 30-31 1.5376197999607797 1103 32-33 1.5240707325439047 1103 34-35 1.9255357128359307 1103 36-37 1.8596445241513635 1103 38-39 1.9052599147549572 1103 40-41 1.5641285419284614 1103 42-43 1.5808424514366592 1103 44-45 1.3331355096437036 1103 46-47 1.2799290510605914 1103 48-49 1.2387625480683866 1103 50-51 1.1994375659474912 1103 52-53 1.2183961551796756 1103 54-55 1.0791345592822452 1103 56-57 1.0432080207431156 1103 58-59 1.005122345263267 1103 60-61 0.9593075167874829 1103 62-63 0.945650420912223 1103 64-65 1.1246897825573434 1103 66-67 1.1618039115879846 1103 68-69 1.1989754940857402 1103 70-71 1.1681727314586432 1103 72-73 1.1379209677142406 1103 74-75 1.0959254832657592 1103 76-77 1.0773618546618202 1103 78-79 1.1212598513741447 1103 80-81 1.08599034193589 1103 82-83 1.1148727606978142 1103 84-85 1.0412455341059648 1103 86-87 1.0153268945769476 1103 88-89 1.0706451884443382 1103 90-91 1.0730963489203624 1103 92-93 1.1128265856513906 1103 94-95 1.0965979643075165 1103 96-97 1.1557006481879988 1103 98-99 1.0327744025021595 1103 100-101 1.1007025718093608 1103 102-103 1.0237763643781648 1103 104-105 1.008364479204019 1103 106-107 1.1118607902826376 1103 108-109 1.1852499760874444 1103 110-111 1.2410977788969575 1103 112-113 1.302436562096851 1103 114-115 1.3402007915930625 1103 116-117 1.3538574530875422 1103 118-119 1.3398743004874696 1103 120-121 1.4019090283814712 1103 122-123 1.3898532561927794 1103 124-125 1.5126447311678852 1104 1 0.7569276945757082 1104 2 0.8131632878356037 1104 3 0.7632561020291639 1104 4 0.8140108146588432 1104 5 0.7809495136274407 1104 6 0.7423978062962746 1104 7 0.8300106314966698 1104 8 0.8573727533881055 1104 9 1.1293288915347546 1104 10-11 1.04674698699543 1104 12-13 0.9825776963310631 1104 14-15 1.0893356498455802 1104 16-17 1.1567378491101366 1104 18-19 1.4160168736103813 1104 20-21 1.632004392316631 1104 22-23 1.458668894789163 1104 24-25 1.8703562036809274 1104 26-27 1.8090677664217694 1104 28-29 1.6276805069769225 1104 30-31 1.5618620210083165 1104 32-33 1.6353953123089298 1104 34-35 1.9386623400672036 1104 36-37 1.982427121768481 1104 38-39 1.9499038768305255 1104 40-41 1.6152696014834973 1104 42-43 1.557578999875389 1104 44-45 1.6010101970495256 1104 46-47 1.467523369473973 1104 48-49 1.4741287819739526 1104 50-51 1.2673770352721903 1104 52-53 1.45182327142318 1104 54-55 1.3087362641009506 1104 56-57 1.2315286118939568 1104 58-59 1.108525593895827 1104 60-61 1.0544884721699148 1104 62-63 1.1248434669327239 1104 64-65 1.092733893866022 1104 66-67 1.1129585868942264 1104 68-69 1.1233995254582148 1104 70-71 1.1445880338493915 1104 72-73 1.1361937044355308 1104 74-75 1.1927247014124447 1104 76-77 1.1710607979230687 1104 78-79 1.2081446792550317 1104 80-81 1.1544215056523512 1104 82-83 1.1856839428244115 1104 84-85 1.0566648787543436 1104 86-87 1.0919384984751908 1104 88-89 1.1096017152692284 1104 90-91 1.0835895855613735 1104 92-93 1.0922450086754552 1104 94-95 1.035010606132051 1104 96-97 0.8647560354248078 1104 98-99 0.9534240582341305 1104 100-101 0.9413826552994315 1104 102-103 0.9661637438620048 1104 104-105 1.0320288969434586 1104 106-107 1.020338225201769 1104 108-109 1.110870740722703 1104 110-111 1.122688202944719 1104 112-113 1.1423862564595595 1104 114-115 1.1035098810662411 1104 116-117 1.1807284826804754 1104 118-119 1.1289610989456662 1104 120-121 1.1678513035567342 1104 122-123 1.220300180653485 1104 124-125 1.4645874250600954 1105 1 0.3010307653656348 1105 2 0.5685509062010823 1105 3 0.6441973851427854 1105 4 0.711661136896808 1105 5 0.7525766964796503 1105 6 0.5703060140181293 1105 7 0.670161687174442 1105 8 0.6019027190682422 1105 9 0.644705704009013 1105 10-11 0.7449809334429887 1105 12-13 0.7696786117956336 1105 14-15 0.8637757115349984 1105 16-17 0.8929676539090678 1105 18-19 0.9563093130714151 1105 20-21 1.3040940080527008 1105 22-23 1.4935467011566672 1105 24-25 1.5787287757391084 1105 26-27 1.3102819842530238 1105 28-29 1.184493650657231 1105 30-31 1.1059858695443978 1105 32-33 1.1699067462880066 1105 34-35 1.7361405874496825 1105 36-37 1.725944135742978 1105 38-39 1.833499882820952 1105 40-41 1.3471037423931946 1105 42-43 0.9331932054155914 1105 44-45 1.0949590077064641 1105 46-47 1.0506702944772783 1105 48-49 0.8997494805598265 1105 50-51 0.9123527896909351 1105 52-53 1.034908430186377 1105 54-55 0.8605996950482364 1105 56-57 0.8173878375424692 1105 58-59 0.7332843452410742 1105 60-61 0.8596859083130965 1105 62-63 0.7798089458780595 1105 64-65 0.8046867130184161 1105 66-67 0.6625128896069619 1105 68-69 0.5765494756721061 1105 70-71 0.7743171476419342 1105 72-73 0.7490446124010894 1105 74-75 0.7937698257021424 1105 76-77 0.8044663107566201 1105 78-79 0.8785806717252918 1105 80-81 0.7813105246866705 1105 82-83 0.771618908478942 1105 84-85 0.9195830766214037 1105 86-87 0.785872786658139 1105 88-89 0.9300614302190375 1105 90-91 0.8008641598187687 1105 92-93 0.791268015639659 1105 94-95 0.9013128330821019 1105 96-97 0.9714643575705928 1105 98-99 0.7222612616665565 1105 100-101 0.7813683303603547 1105 102-103 0.7411846313497747 1105 104-105 0.8240695569441776 1105 106-107 0.7715469925327127 1105 108-109 0.8436636392695043 1105 110-111 0.8310416799615297 1105 112-113 0.8662926812459517 1105 114-115 0.8364583383551683 1105 116-117 0.8271228486921807 1105 118-119 0.8892009063271793 1105 120-121 0.8470417323318635 1105 122-123 0.9787074859334481 1105 124-125 1.1208502145310462 1106 1 0.37861673380091077 1106 2 0.6213602560261648 1106 3 0.4398834552782418 1106 4 0.555871818711779 1106 5 0.4081891575769774 1106 6 0.45649818381035345 1106 7 0.2625384698703428 1106 8 0.5645454112509825 1106 9 0.693625080718725 1106 10-11 0.7410684485685337 1106 12-13 0.718624296364986 1106 14-15 0.9816923056147289 1106 16-17 0.8124455929737024 1106 18-19 0.9168164044226614 1106 20-21 1.2666570998324076 1106 22-23 1.2556874546796806 1106 24-25 1.457684224232196 1106 26-27 1.60246585735851 1106 28-29 1.5150648549098022 1106 30-31 1.3054204859793401 1106 32-33 1.3274142413795573 1106 34-35 1.588441818916472 1106 36-37 1.4870696530720267 1106 38-39 1.603593393431197 1106 40-41 1.3251440363820564 1106 42-43 1.270425462618178 1106 44-45 1.0608068299806845 1106 46-47 0.837216222585603 1106 48-49 0.9635419939432879 1106 50-51 0.8748307634795935 1106 52-53 0.7493440442012229 1106 54-55 0.6839341044338454 1106 56-57 0.7311536016592584 1106 58-59 0.6648124022496731 1106 60-61 0.7185174626779656 1106 62-63 0.7135926664332644 1106 64-65 0.7864080474566499 1106 66-67 0.5709115855140077 1106 68-69 0.5918312081299995 1106 70-71 0.7335526188763204 1106 72-73 0.7188774574401435 1106 74-75 0.7385148601672071 1106 76-77 0.6010604287429544 1106 78-79 0.5829474968668968 1106 80-81 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98-99 -2.9293680125843373 2216 100-101 -2.907361073328129 2216 102-103 -2.8543061409579913 2216 104-105 -2.9958607850198895 2216 106-107 -2.911572139120878 2216 108-109 -2.987898135238428 2216 110-111 -3.0564272308419405 2216 112-113 -3.054220246856982 2216 114-115 -3.1839814999180227 2216 116-117 -3.2466572988755544 2216 118-119 -3.2717314474442745 2216 120-121 -3.3289605212187325 2216 122-123 -3.366593505957539 2216 124-125 -3.3142733612385378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 172694.0 3 30297.0 4 19285.0 5 15087.0 6 14061.0 7 15058.0 8 16034.0 9 16960.0 10 17904.0 11 19351.0 12 20739.0 13 22491.0 14 25743.0 15 23156.0 16 18483.0 17 16424.0 18 15466.0 19 14776.0 20 14543.0 21 15846.0 22 18603.0 23 24744.0 24 32524.0 25 42150.0 26 52362.0 27 61352.0 28 70700.0 29 83513.0 30 97180.0 31 112282.0 32 139004.0 33 176562.0 34 233791.0 35 322987.0 36 513955.0 37 744650.0 38 277551.0 39 950.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.29076529965969 18.86615849399224 13.07232123298987 23.770754973358198 2 14.91348522062799 19.98507838241278 41.19219360812988 23.909242788829356 3 19.205876568929764 25.25382559015335 28.639489169446964 26.900808671469917 4 12.308458703928927 16.364220612954817 37.91508878992486 33.41223189319139 5 13.610003706671897 38.11008475469653 34.40239267371199 13.87751886491958 6 31.84170484779966 36.738750378977855 17.88188925062974 13.537655522592749 7 28.65020409377688 32.09833338764499 21.29673577471334 17.95472674386479 8 25.797575581042814 35.96481186697034 19.928551553896032 18.309060998090818 9 26.55084183861407 16.319399973931624 19.88512912347911 37.2446290639752 10-11 24.74235377521281 26.852981561563364 27.882390576149437 20.522274087074393 12-13 25.596436871191624 24.02745933824491 28.039988716815444 22.336115073748022 14-15 22.643661124429272 25.436880189559652 25.624716778528818 26.294741907482262 16-17 22.553885594394295 28.842473872953363 26.103011669573018 22.500628863079324 18-19 22.46155132181278 27.69299430017384 27.994335594089637 21.85111878392374 20-21 23.266360479139653 26.888272911924993 27.967759632212257 21.8776069767231 22-23 22.927814153705263 26.84339435950851 27.670664933316257 22.558126553469968 24-25 22.755905444871463 26.914880774595325 27.80373672872444 22.52547705180877 26-27 22.587997538962416 27.232922584157482 27.587539849452742 22.591540027427364 28-29 22.445460021772355 27.281067967492206 27.786445036701892 22.487026974033547 30-31 22.650157995430146 27.065770414732338 27.682507956441317 22.6015636333962 32-33 22.500103784073584 27.148921857039245 27.50571237458952 22.845261984297647 34-35 22.904378395671678 26.91101948148184 27.723472849220204 22.46112927362628 36-37 22.797777696857754 26.811678497441736 27.555161912701877 22.835381892998637 38-39 22.592372474946927 27.083515441987444 27.803723587751815 22.520388495313817 40-41 22.890438067760318 26.95581628177704 27.575815332336184 22.577930318126455 42-43 22.64926342205782 27.040393797479993 27.420311678883103 22.89003110157908 44-45 22.78538480220524 27.110497032462256 27.33269041169184 22.771427753640662 46-47 22.80947105809774 27.278475533846088 27.01940322127504 22.892650186781136 48-49 22.792303785529015 27.32250798099073 26.97858282998676 22.906605403493497 50-51 22.758838777390775 27.286158428702095 27.032751618822658 22.92225117508447 52-53 23.014554334083822 27.24678955179814 26.916507662517162 22.822148451600874 54-55 22.916191093992147 27.13393444302416 26.981198566416726 22.968675896566964 56-57 22.76307877841294 27.448730757918277 26.992341420537848 22.79584904313094 58-59 22.860481085829818 27.173895950281725 26.97342817324553 22.992194790642927 60-61 22.970148589744788 27.098423881581574 27.109191459723732 22.822236068949902 62-63 22.862617341726573 27.169742283607494 27.095273340699457 22.87236703396648 64-65 22.796915321540776 27.077739859765703 27.054260731959328 23.071084086734196 66-67 22.71741471154936 27.386461521024597 26.851085231780576 23.045038535645475 68-69 22.726941658492787 27.152979566203022 27.12798322921499 22.992095546089192 70-71 22.63907847955644 27.178180123216027 27.141117558177676 23.04162383904986 72-73 22.79153034925124 27.113579977527998 26.95986069056785 23.135028982652916 74-75 22.74447567859184 27.26600811445582 26.953350237741258 23.036165969211083 76-77 22.840017358663683 27.19312873477982 26.93775009219842 23.029103814358074 78-79 22.791366771935305 27.24842242254637 26.915247244826734 23.044963560691592 80-81 22.693516799893004 27.297932969307436 27.037926613659486 22.970623617140074 82-83 22.800597187810975 27.35744110293301 26.89097667238655 22.95098503686946 84-85 22.81367641583585 27.208807063694408 26.877590133676826 23.09992638679292 86-87 22.76850248628565 27.072411218909743 27.13856198797442 23.02052430683019 88-89 22.845829841705637 27.092645718128512 26.955597264585418 23.105927175580437 90-91 22.738091189407335 27.34092300367297 27.01853205913028 22.902453747789416 92-93 22.833488920621864 27.18115361657918 27.037391812051876 22.947965650747083 94-95 22.728329737972523 27.033353318844195 27.10612022850652 23.132196714676766 96-97 22.83299918651344 26.975701818137505 27.01689334285731 23.174405652491746 98-99 22.571714226332716 27.028404326321848 26.957964101836847 23.441917345508593 100-101 22.53498556938244 26.955245958081075 27.248406397927997 23.26136207460849 102-103 22.73598303099405 26.950679151255024 27.067247563085502 23.24609025466543 104-105 22.688787519278346 26.818369729960086 27.254287705013493 23.238555045748083 106-107 22.477631626482342 26.925436865969683 27.29112284949455 23.30580865805343 108-109 22.546835623805343 26.92830900999587 27.010096739881302 23.514758626317487 110-111 22.440536705394596 26.94778699979259 27.221458128614806 23.39021816619801 112-113 22.565918955202484 26.968062408585602 27.305102092281153 23.160916543930764 114-115 22.541324550372913 27.005039019533285 27.086288392630976 23.367348037462833 116-117 22.309703626087977 27.031857687933268 27.21229221553086 23.446146470447896 118-119 22.480249389531735 27.003041432505075 27.036688731739076 23.480020446224106 120-121 22.388051877767488 26.968902099095914 27.2090942641618 23.4339517589748 122-123 22.411297218848837 27.078978074144764 27.171844620030612 23.33788008697579 124-125 22.456375730507506 26.892737316011868 27.17259071925116 23.47829623422946 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 257.0 1 273.5 2 354.5 3 430.5 4 391.5 5 351.5 6 362.5 7 468.0 8 694.5 9 839.0 10 847.5 11 893.5 12 1021.5 13 1148.0 14 1307.5 15 1538.5 16 1728.0 17 1870.0 18 2144.0 19 2528.5 20 3012.0 21 3675.5 22 4718.0 23 6312.5 24 8241.0 25 11102.5 26 15567.0 27 21113.5 28 26300.5 29 31700.5 30 38434.0 31 45120.5 32 52015.5 33 59929.0 34 69303.0 35 76954.5 36 84228.5 37 92775.5 38 98587.0 39 101980.5 40 105218.0 41 110384.0 42 115795.0 43 121976.5 44 127686.5 45 129671.5 46 129383.5 47 130940.5 48 136827.5 49 140141.5 50 138362.5 51 134996.0 52 131450.5 53 128818.5 54 124035.0 55 114473.5 56 104377.0 57 95766.0 58 87616.0 59 78415.5 60 66305.0 61 52966.0 62 40623.0 63 29922.5 64 21634.5 65 15860.5 66 11640.0 67 8887.5 68 6673.5 69 4843.5 70 3554.0 71 2516.0 72 1848.0 73 1431.0 74 1064.5 75 809.5 76 543.0 77 347.5 78 247.5 79 175.0 80 129.5 81 100.0 82 82.5 83 60.5 84 45.0 85 33.0 86 32.0 87 33.5 88 21.0 89 15.0 90 14.5 91 14.0 92 12.5 93 8.0 94 6.5 95 5.5 96 4.0 97 2.5 98 4.5 99 5.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011475499949281124 2 0.004250185166400416 3 0.007366987621760721 4 0.007196980215104704 5 0.01377059993913735 6 7.933678977280776E-4 7 0.006885299969568675 8 0.0 9 0.0022100962865282165 10-11 0.0 12-13 0.003187638874800312 14-15 0.01867248016438583 16-17 0.030190481965330956 18-19 0.005751917258528563 20-21 0.007536995028416739 22-23 0.001756743202112172 24-25 0.0112204888392971 26-27 0.018842487571041847 28-29 3.4001481331203325E-4 30-31 0.0011900518465921166 32-33 0.008401199345584823 34-35 0.008982057984992879 36-37 0.0112346561231851 38-39 1.4167283888001386E-5 40-41 0.024523568410130404 42-43 0.009562916624400937 44-45 0.016292376471201596 46-47 0.0036126573914403537 48-49 0.024764412236226423 50-51 0.004250185166400416 52-53 0.0 54-55 0.01749659560168171 56-57 0.004080177759744399 58-59 3.541820972000347E-4 60-61 0.004108512327520402 62-63 0.02667699556110661 64-65 0.016774064123393644 66-67 0.001643404931008161 68-69 0.020995914722018057 70-71 0.002564278383728251 72-73 0.011985522169249174 74-75 2.833456777600277E-5 76-77 0.005227727754672512 78-79 9.49208020496093E-4 80-81 0.0032159734425763146 82-83 0.0014167283888001387 84-85 0.0 86-87 0.005624411703536551 88-89 0.016405714742305604 90-91 0.02204429372973016 92-93 0.004434359856944434 94-95 0.02460857211345841 96-97 0.017553264737233717 98-99 6.94196910512068E-4 100-101 0.008585374036128841 102-103 0.0 104-105 0.0011050481432641082 106-107 1.4167283888001386E-5 108-109 0.0 110-111 6.23360491072061E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.4167283888001386E-5 122-123 0.0 124-125 0.02276682520801823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3529258.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.08664258711788 #Duplication Level Percentage of deduplicated Percentage of total 1 72.98379462909917 37.284970308684024 2 13.39275989576755 13.683822761003258 3 5.286413023801936 8.101952781445638 4 2.673198099378158 5.462588634699791 5 1.4920984441351728 3.8113149960164128 6 0.9786694967227885 2.9998163273994978 7 0.6283478558160324 2.247012763131887 8 0.47206178329517656 1.9292841281790545 9 0.33243621157205344 1.5284744931237295 >10 1.616025402686801 14.627829636081877 >50 0.09689386471065616 3.399065716688497 >100 0.045663575390065575 4.1491538219412005 >500 0.0012988794966664787 0.4643434968234527 >1k 3.388381278088685E-4 0.31037013478155884 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8334567776002775E-5 0.0 5 0.0 0.0 0.0 2.8334567776002775E-5 0.0 6 2.8334567776002775E-5 0.0 0.0 2.8334567776002775E-5 0.0 7 2.8334567776002775E-5 0.0 0.0 8.500370332800833E-5 0.0 8 2.8334567776002775E-5 0.0 0.0 2.5501110998402495E-4 0.0 9 2.8334567776002775E-5 0.0 0.0 3.683493810880361E-4 0.0 10-11 5.666913555200555E-5 0.0 0.0 4.250185166400416E-4 0.0 12-13 8.500370332800833E-5 0.0 0.0 5.80858639408057E-4 0.0 14-15 8.500370332800833E-5 0.0 0.0 6.516950588480638E-4 0.0 16-17 9.917098721600972E-5 0.0 0.0 7.225314782880708E-4 5.666913555200555E-5 18-19 1.5584012276801526E-4 0.0 0.0 7.650333299520749E-4 5.666913555200555E-5 20-21 1.7000740665601665E-4 0.0 0.0 8.217024655040805E-4 5.666913555200555E-5 22-23 2.266765422080222E-4 0.0 0.0 9.633753043840944E-4 5.666913555200555E-5 24-25 3.400148133120333E-4 1.4167283888001387E-5 0.0 0.001090880859376107 5.666913555200555E-5 26-27 3.400148133120333E-4 2.8334567776002775E-5 0.0 0.0013033901176961278 5.666913555200555E-5 28-29 3.966839488640389E-4 4.2501851664004164E-5 0.0 0.0019692524604321927 5.666913555200555E-5 30-31 4.958549360800485E-4 7.083641944000693E-5 0.0 0.00398100677252839 5.666913555200555E-5 32-33 5.100222199680499E-4 8.500370332800833E-5 0.0 0.008146188235600798 5.666913555200555E-5 34-35 5.950259232960582E-4 8.500370332800833E-5 0.0 0.01389810549412936 5.666913555200555E-5 36-37 6.23360491072061E-4 8.500370332800833E-5 0.0 0.02443856470680239 5.666913555200555E-5 38-39 6.516950588480638E-4 8.500370332800833E-5 0.0 0.04407442017557232 5.666913555200555E-5 40-41 6.516950588480638E-4 8.500370332800833E-5 0.0 0.0673937694552226 5.666913555200555E-5 42-43 6.94196910512068E-4 8.500370332800833E-5 0.0 0.09047227490877685 5.666913555200555E-5 44-45 7.366987621760722E-4 8.500370332800833E-5 0.0 0.1184668278714676 5.666913555200555E-5 46-47 8.217024655040805E-4 8.500370332800833E-5 0.0 0.15277998944820698 5.666913555200555E-5 48-49 8.217024655040805E-4 8.500370332800833E-5 0.0 0.19062080471305864 5.666913555200555E-5 50-51 8.217024655040805E-4 8.500370332800833E-5 0.0 0.23421353723643895 5.666913555200555E-5 52-53 8.358697493920819E-4 8.500370332800833E-5 0.0 0.2762337012482511 5.666913555200555E-5 54-55 8.500370332800832E-4 8.500370332800833E-5 0.0 0.32044979426270337 5.666913555200555E-5 56-57 9.633753043840944E-4 8.500370332800833E-5 0.0 0.37448381501154065 5.666913555200555E-5 58-59 9.633753043840944E-4 8.500370332800833E-5 0.0 0.4361398344921227 5.666913555200555E-5 60-61 9.633753043840944E-4 8.500370332800833E-5 0.0 0.5044828119678414 5.666913555200555E-5 62-63 9.633753043840944E-4 8.500370332800833E-5 0.0 0.5734208153668562 5.666913555200555E-5 64-65 9.91709872160097E-4 8.500370332800833E-5 0.0 0.6468923496100314 5.666913555200555E-5 66-67 0.0010200444399360998 8.500370332800833E-5 0.0 0.7312585251630797 5.666913555200555E-5 68-69 0.0010483790077121028 8.500370332800833E-5 0.0 0.8281769142408971 5.666913555200555E-5 70-71 0.0011475499949281124 8.500370332800833E-5 0.0 0.9410618322604922 5.666913555200555E-5 72-73 0.0012183864143681194 8.500370332800833E-5 0.0 1.0543859360806151 5.666913555200555E-5 74-75 0.001246720982144122 8.500370332800833E-5 0.0 1.172852763952083 5.666913555200555E-5 76-77 0.001246720982144122 8.500370332800833E-5 0.0 1.3020158911589914 5.666913555200555E-5 78-79 0.001246720982144122 8.500370332800833E-5 0.0 1.451693245435726 5.666913555200555E-5 80-81 0.0015584012276801526 8.500370332800833E-5 0.0 1.6192072101274546 5.666913555200555E-5 82-83 0.0016717394987841637 8.500370332800833E-5 0.0 1.7996984068605921 5.666913555200555E-5 84-85 0.0017000740665601664 8.500370332800833E-5 0.0 1.9846522980184504 5.666913555200555E-5 86-87 0.0017284086343361692 8.500370332800833E-5 0.0 2.1798349681434455 5.666913555200555E-5 88-89 0.0017284086343361692 8.500370332800833E-5 0.0 2.4037630572771955 5.666913555200555E-5 90-91 0.001870081473216183 1.133382711040111E-4 0.0 2.656804914800788 5.666913555200555E-5 92-93 0.001898416040992186 1.133382711040111E-4 0.0 2.926776676570543 5.666913555200555E-5 94-95 0.001898416040992186 1.133382711040111E-4 0.0 3.204002087690954 5.666913555200555E-5 96-97 0.001898416040992186 1.133382711040111E-4 0.0 3.4889203339625494 5.666913555200555E-5 98-99 0.0020259215959841986 1.133382711040111E-4 0.0 3.7979796319792998 5.666913555200555E-5 100-101 0.002054256163760201 1.133382711040111E-4 0.0 4.145913956984726 5.666913555200555E-5 102-103 0.0021109252993122066 1.133382711040111E-4 0.0 4.526036350983691 5.666913555200555E-5 104-105 0.0025501110998402497 1.133382711040111E-4 0.0 4.912590691867809 5.666913555200555E-5 106-107 0.0026209475192802566 1.133382711040111E-4 0.0 5.302474344465606 5.666913555200555E-5 108-109 0.0026634493709442606 1.4167283888001387E-4 0.0 5.715138989555312 5.666913555200555E-5 110-111 0.002748453074272269 1.4167283888001387E-4 0.0 6.1836510677315175 5.666913555200555E-5 112-113 0.002819289493712276 1.4167283888001387E-4 0.0 6.683175330338559 5.666913555200555E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7985 0.0 55.961 1 GGTATCA 3905 0.0 55.91978 1 ATCAACG 9205 0.0 47.703804 3 TCAACGC 9345 0.0 46.35243 4 CAACGCA 9440 0.0 45.88596 5 AACGCAG 9920 0.0 43.53892 6 TATCAAC 10010 0.0 43.332516 2 ACGCAGA 11885 0.0 35.78983 7 CGCAGAG 11935 0.0 34.74512 8 GTACATG 14515 0.0 34.72057 1 TACATGG 15160 0.0 32.065887 2 ACATGGG 15130 0.0 31.067665 3 GCAGAGT 13800 0.0 28.669891 9 CATGGGG 7145 0.0 25.399084 4 AGAGTAC 12535 0.0 23.950394 10-11 CATGGGA 9230 0.0 23.593904 4 CAGAGTA 13460 0.0 21.994993 10-11 GAGTACT 5610 0.0 21.376534 12-13 GAGTACA 11055 0.0 20.452448 1 ATGGGGA 4830 0.0 19.587112 5 >>END_MODULE