FastQCFastQC Report
Thu 26 May 2016
SRR1364568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529258
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60530.1715091387481448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.14827479317182252No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50750.068.493951
GTATCAA102450.053.2511331
TCAACGC125850.043.1156544
ATCAACG126100.043.0301743
CAACGCA130250.041.6599775
TATCAAC132600.040.920852
AACGCAG134000.040.3609166
ACGCAGA160800.033.2640847
GTACATG140450.033.197451
CGCAGAG162500.032.733028
TACATGG147050.030.824032
ACATGGG146050.030.0563143
GCAGAGT179500.028.936479
GAGTACT101900.026.18689712-13
AGAGTAC167300.024.73309710-11
CATGGGG75950.024.624744
GTACTTT109400.024.36444314-15
CAGAGTA176600.023.90226210-11
AGTACTT113800.022.69045612-13
CATGGGA87850.022.5773144
Produced by FastQC (version 0.11.5)