##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364568_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3529258 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.19504014724908 31.0 30.0 34.0 26.0 34.0 2 30.091681877607133 31.0 30.0 34.0 26.0 34.0 3 30.394221674924303 31.0 30.0 34.0 26.0 34.0 4 33.96098698366625 35.0 35.0 37.0 30.0 37.0 5 33.3883547193206 35.0 35.0 37.0 27.0 37.0 6 33.410148535471194 36.0 35.0 37.0 28.0 37.0 7 33.207607944786126 36.0 33.0 37.0 26.0 37.0 8 33.14327600872478 36.0 33.0 37.0 26.0 37.0 9 34.41591178655683 38.0 34.0 39.0 26.0 39.0 10-11 34.355557315447044 38.0 34.0 39.0 26.0 39.0 12-13 34.23778284273918 38.0 34.0 39.0 25.5 39.0 14-15 35.24038636449928 39.0 34.0 41.0 25.0 41.0 16-17 35.229780169089366 39.0 34.0 41.0 25.0 41.0 18-19 35.23659222420123 39.0 34.0 41.0 25.0 41.0 20-21 35.23934351073228 39.0 34.0 41.0 25.0 41.0 22-23 35.198125214988536 39.0 34.0 41.0 25.0 41.0 24-25 35.153328688353184 39.0 34.0 41.0 25.0 41.0 26-27 35.044028376502936 39.0 34.0 41.0 25.0 41.0 28-29 34.902982723280644 39.0 34.0 41.0 24.0 41.0 30-31 34.768074054092956 39.0 34.0 41.0 24.0 41.0 32-33 34.597881481036524 38.5 34.0 41.0 24.0 41.0 34-35 34.43284792440791 38.0 33.0 41.0 23.0 41.0 36-37 34.25419124926543 38.0 33.0 40.0 22.0 41.0 38-39 34.10460739906235 38.0 33.0 40.0 20.5 41.0 40-41 34.151696617249286 38.0 33.0 40.5 21.0 41.0 42-43 34.04414398153946 38.0 33.0 41.0 19.0 41.0 44-45 33.904927041321436 38.0 33.0 41.0 18.0 41.0 46-47 33.70157169013997 38.0 33.0 41.0 15.5 41.0 48-49 33.46684444718975 38.0 33.0 40.0 13.5 41.0 50-51 33.30671900439129 38.0 32.0 40.0 10.5 41.0 52-53 33.13150625428914 38.0 32.0 40.0 9.0 41.0 54-55 32.94523282230996 38.0 32.0 40.0 8.0 41.0 56-57 32.73234940602246 38.0 32.0 40.0 7.5 41.0 58-59 32.530614792117774 37.0 31.0 40.0 6.5 41.0 60-61 32.296917085687696 37.0 31.0 40.0 2.0 41.0 62-63 32.121547928771434 36.5 31.0 40.0 2.0 41.0 64-65 31.976949262422867 36.0 31.0 39.5 2.0 41.0 66-67 31.67911711186884 36.0 31.0 39.0 2.0 41.0 68-69 31.390811326346785 35.0 31.0 39.0 2.0 41.0 70-71 31.071313290215677 35.0 30.0 38.5 2.0 40.5 72-73 30.773686848623704 35.0 30.0 37.5 2.0 40.0 74-75 30.412134363653777 35.0 30.0 37.0 2.0 39.5 76-77 30.095268042177704 35.0 30.0 36.5 2.0 39.0 78-79 29.80508693328739 35.0 30.0 36.0 2.0 39.0 80-81 29.516699260864463 34.5 29.0 36.0 2.0 37.5 82-83 29.22811494654117 34.0 29.0 35.0 2.0 37.0 84-85 29.00085032037896 34.0 29.0 35.0 2.0 37.0 86-87 28.78962985987423 34.0 29.0 35.0 2.0 36.0 88-89 28.61257054599012 34.0 29.0 35.0 2.0 36.0 90-91 28.47233285296796 34.0 29.0 35.0 2.0 36.0 92-93 28.356573676393168 34.0 29.0 35.0 2.0 35.0 94-95 28.246146357109623 34.0 29.0 35.0 2.0 35.0 96-97 28.135326179043865 34.0 29.0 35.0 2.0 35.0 98-99 28.055383454539168 34.0 29.0 35.0 2.0 35.0 100-101 27.95994512160913 34.0 28.5 35.0 2.0 35.0 102-103 26.680188441876453 32.0 25.0 34.0 2.0 35.0 104-105 27.626242541633395 34.0 27.0 35.0 2.0 35.0 106-107 27.791402187088618 34.0 28.0 35.0 2.0 35.0 108-109 27.80238126541046 34.0 28.0 35.0 2.0 35.0 110-111 27.72782919242515 34.0 28.0 35.0 2.0 35.0 112-113 27.64479134140944 34.0 27.5 35.0 2.0 35.0 114-115 27.547450200580407 34.0 27.0 35.0 2.0 35.0 116-117 27.42683178730487 34.0 27.0 35.0 2.0 35.0 118-119 27.29577959446433 34.0 27.0 35.0 2.0 35.0 120-121 27.101053677571883 34.0 27.0 35.0 2.0 35.0 122-123 26.813253664084634 34.0 26.5 35.0 2.0 35.0 124-125 24.968242616436655 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5774171594594577 1101 2 0.6403617988131352 1101 3 0.5525561838745539 1101 4 0.4871878846812123 1101 5 0.6105371920550908 1101 6 0.585811570243223 1101 7 0.6674543357514438 1101 8 0.7022948535551023 1101 9 0.881805195836499 1101 10-11 0.8725721807397235 1101 12-13 0.7624027820993433 1101 14-15 0.9206356815249919 1101 16-17 0.9372617426412475 1101 18-19 1.0785640160291123 1101 20-21 1.0796772467023743 1101 22-23 0.9780984312911869 1101 24-25 0.9720340162111683 1101 26-27 0.9700606318236353 1101 28-29 1.097402948838365 1101 30-31 1.0886431270534018 1101 32-33 1.0412090246977925 1101 34-35 1.1257752234497431 1101 36-37 1.1108260271973691 1101 38-39 1.1425348823842754 1101 40-41 0.909702438351438 1101 42-43 0.8505322690701576 1101 44-45 0.9117054399615583 1101 46-47 0.948970665851327 1101 48-49 1.0555433960453442 1101 50-51 1.081248925548202 1101 52-53 1.0459568040347875 1101 54-55 1.0668985643259887 1101 56-57 1.0476287765997156 1101 58-59 1.0576818566443933 1101 60-61 1.0098423147383855 1101 62-63 1.057248319783966 1101 64-65 0.8676193371940677 1101 66-67 0.974986247187644 1101 68-69 1.086169371576279 1101 70-71 1.0454848005416686 1101 72-73 0.9459445857268598 1101 74-75 1.0327662227233319 1101 76-77 1.0387006250918915 1101 78-79 0.9176970909744782 1101 80-81 0.8018692177684272 1101 82-83 0.9711732143584939 1101 84-85 0.9804910973827248 1101 86-87 1.0053570945598551 1101 88-89 0.9927793892673868 1101 90-91 0.9539427230549222 1101 92-93 0.8226055153820191 1101 94-95 0.925931641753106 1101 96-97 0.9255453386750965 1101 98-99 0.9362758816787604 1101 100-101 0.9032592864868967 1101 102-103 0.7919564196285336 1101 104-105 0.8431120671813837 1101 106-107 0.8071880073363928 1101 108-109 0.8957179295521485 1101 110-111 0.7987607765221938 1101 112-113 0.7915630612284374 1101 114-115 0.8804337389795407 1101 116-117 0.8783374614647208 1101 118-119 0.8315083837736807 1101 120-121 0.8337235597436781 1101 122-123 0.7709448448701899 1101 124-125 0.9532767144059484 1102 1 0.3659085791451844 1102 2 -0.40923880252154987 1102 3 0.21678656212506198 1102 4 0.36534223241694974 1102 5 0.5458822068923794 1102 6 0.30908713007881516 1102 7 0.3676686352028753 1102 8 0.49186927682399073 1102 9 0.7517941615046766 1102 10-11 0.7804888735304445 1102 12-13 0.7783041724157655 1102 14-15 0.9442180728072387 1102 16-17 0.9322862546380009 1102 18-19 1.027102325167455 1102 20-21 0.8285249703401405 1102 22-23 1.033464465133406 1102 24-25 0.9396891467293074 1102 26-27 1.0001851913250803 1102 28-29 0.6646504384045926 1102 30-31 0.9111777734229065 1102 32-33 1.0739386516321758 1102 34-35 1.0615985726970365 1102 36-37 0.7423085797325513 1102 38-39 0.22718868403917014 1102 40-41 -0.10808381731865069 1102 42-43 0.09575297566658492 1102 44-45 -0.12052636225550373 1102 46-47 0.16231303752116588 1102 48-49 -0.14862279804007983 1102 50-51 -0.26962421673784576 1102 52-53 -0.575764685578875 1102 54-55 -0.35890576895639015 1102 56-57 -0.3818618078206697 1102 58-59 -0.3077525999176913 1102 60-61 -0.4030056606296242 1102 62-63 -0.3874118291360773 1102 64-65 -0.47894944116520577 1102 66-67 -0.5552129273156581 1102 68-69 -0.3126419427584608 1102 70-71 -0.8840390700361134 1102 72-73 -0.6630067018897279 1102 74-75 -0.5241352598785163 1102 76-77 -0.7882879674425709 1102 78-79 -0.6212859306196101 1102 80-81 -0.5908111033848726 1102 82-83 -0.8610154156537142 1102 84-85 -0.8023912819000678 1102 86-87 -0.4690907015912522 1102 88-89 -0.5762858062802749 1102 90-91 -0.6079490413979016 1102 92-93 -0.445882567469166 1102 94-95 -0.16831861191441888 1102 96-97 -0.40701084800635456 1102 98-99 -0.5360255150248427 1102 100-101 -0.4252369282840789 1102 102-103 -0.48910402685918086 1102 104-105 -0.38855765100990425 1102 106-107 -0.24079853824615327 1102 108-109 -0.2106977982335394 1102 110-111 -0.07783604909379704 1102 112-113 0.03924418430221621 1102 114-115 -0.007132078966428423 1102 116-117 -0.10510693139694283 1102 118-119 0.06672721648273594 1102 120-121 0.22580155995361295 1102 122-123 0.18356645088560342 1102 124-125 0.44619744732603905 1103 1 0.17992554965218233 1103 2 -0.21078706260906444 1103 3 0.16779061317080846 1103 4 0.12862498360581753 1103 5 0.11999383031409394 1103 6 0.2521316449362203 1103 7 0.5031073604585785 1103 8 0.44932068307614514 1103 9 0.6622982265917656 1103 10-11 0.5994436361389717 1103 12-13 0.52582980262207 1103 14-15 0.6268109515152815 1103 16-17 0.4799851645063953 1103 18-19 0.5874429633297282 1103 20-21 0.5975462270258447 1103 22-23 0.6194394744030731 1103 24-25 0.6186079539864409 1103 26-27 0.6702017886656009 1103 28-29 0.706618577412911 1103 30-31 0.6087345563661515 1103 32-33 0.7767507037669574 1103 34-35 0.7458998541716468 1103 36-37 0.770933042413283 1103 38-39 0.8009797365161759 1103 40-41 0.7151139290223867 1103 42-43 0.7096535934379773 1103 44-45 0.7603772479873498 1103 46-47 0.7495724724690618 1103 48-49 0.6548377022409824 1103 50-51 0.6340393356070777 1103 52-53 0.5987290764960775 1103 54-55 0.6579659726791363 1103 56-57 0.6543202279262061 1103 58-59 0.7785891501283544 1103 60-61 0.7322658774778645 1103 62-63 0.6903505428318937 1103 64-65 0.6428513345831597 1103 66-67 0.5756182153305573 1103 68-69 0.7131013082354976 1103 70-71 0.6477546898017472 1103 72-73 0.6390640626588855 1103 74-75 0.667380533773752 1103 76-77 0.6557550295221226 1103 78-79 0.6351565458159278 1103 80-81 0.6120862677429884 1103 82-83 0.6062109055293377 1103 84-85 0.656489247234763 1103 86-87 0.6831923965853228 1103 88-89 0.6376962512663731 1103 90-91 0.6998615375860666 1103 92-93 0.6706151033342245 1103 94-95 0.582326099807343 1103 96-97 0.6421957210563356 1103 98-99 0.6630677237602889 1103 100-101 0.6393283684713253 1103 102-103 0.4729356849077959 1103 104-105 0.4965311116814455 1103 106-107 0.6191334969057465 1103 108-109 0.6454109464753763 1103 110-111 0.5490388863517346 1103 112-113 0.5411459529875522 1103 114-115 0.5798877012366113 1103 116-117 0.7338535286640102 1103 118-119 0.5691805242655867 1103 120-121 0.5600560290267289 1103 122-123 0.6339249572487127 1103 124-125 0.8720689266978816 1104 1 0.29462471233004806 1104 2 0.51661781246316 1104 3 0.18869647226510367 1104 4 0.02389847394310607 1104 5 0.3353540740341785 1104 6 0.33029391712383926 1104 7 0.5044099277820422 1104 8 0.3520589205385747 1104 9 0.484450439142023 1104 10-11 0.529011217761763 1104 12-13 0.48295864488088824 1104 14-15 0.5091467774065208 1104 16-17 0.49975551465285406 1104 18-19 0.42890827147596866 1104 20-21 0.38028845794525523 1104 22-23 0.4476256812559427 1104 24-25 0.4992591333414609 1104 26-27 0.3993205161535158 1104 28-29 0.5999022123230304 1104 30-31 0.5432112589031561 1104 32-33 0.6882389519394465 1104 34-35 0.76873186709377 1104 36-37 0.7492675883121507 1104 38-39 0.7979737288220647 1104 40-41 0.7520545237420109 1104 42-43 0.8067569401560988 1104 44-45 0.8061773754650545 1104 46-47 0.7521832694803763 1104 48-49 0.7154405540610114 1104 50-51 0.5599546176952757 1104 52-53 0.7009764018670808 1104 54-55 0.27884696579825174 1104 56-57 0.6219183907869734 1104 58-59 0.7082116790371487 1104 60-61 0.7796310712964996 1104 62-63 0.8450100354318835 1104 64-65 0.7687081117814181 1104 66-67 0.7327030625081932 1104 68-69 0.4891370459503115 1104 70-71 0.1009062426120515 1104 72-73 0.14165241510083604 1104 74-75 0.5683046912455936 1104 76-77 0.6854963376996572 1104 78-79 0.6945334584937015 1104 80-81 0.6449936012871724 1104 82-83 0.6127307269711473 1104 84-85 0.5964842591387658 1104 86-87 0.5530164460624682 1104 88-89 0.539277224380946 1104 90-91 0.5930330241541562 1104 92-93 0.5491523767791087 1104 94-95 0.5797724107210662 1104 96-97 0.5967315170711487 1104 98-99 0.6157296124344818 1104 100-101 0.5762461460976098 1104 102-103 0.4512299609651933 1104 104-105 0.5904780255252149 1104 106-107 0.5522947427898259 1104 108-109 0.5749766468521109 1104 110-111 0.6423839504649713 1104 112-113 0.6051600440793443 1104 114-115 0.5842214402485517 1104 116-117 0.5543758929544609 1104 118-119 0.537898047159981 1104 120-121 0.550400766436443 1104 122-123 0.684436041513262 1104 124-125 1.1462805262748468 1105 1 0.19079557124841884 1105 2 -0.07369968466709764 1105 3 0.1825509417119413 1105 4 0.2260239130010291 1105 5 0.03107737005780109 1105 6 0.2991496485992755 1105 7 0.42070546049345836 1105 8 0.4607147905810862 1105 9 0.6711734227678363 1105 10-11 0.5642405082383135 1105 12-13 0.5573798493024498 1105 14-15 0.6381295201714394 1105 16-17 0.5415585108916474 1105 18-19 0.564087775838928 1105 20-21 0.5757792297726851 1105 22-23 0.6046970652513792 1105 24-25 0.5773063105310001 1105 26-27 0.6189170584146453 1105 28-29 0.6044695852623505 1105 30-31 0.702768839232192 1105 32-33 0.7358933711977897 1105 34-35 0.7820729908625523 1105 36-37 0.7197644507993175 1105 38-39 0.8069380459694955 1105 40-41 0.5643138695210226 1105 42-43 0.6380500000230711 1105 44-45 0.5731651765869046 1105 46-47 0.5404596456951154 1105 48-49 0.5311203631398627 1105 50-51 0.3637296195791677 1105 52-53 0.4001541628015559 1105 54-55 0.3730049439772216 1105 56-57 0.3505329687170615 1105 58-59 0.11561091611342533 1105 60-61 0.23427813494004823 1105 62-63 0.4698331868160537 1105 64-65 0.23767969382388898 1105 66-67 0.4205929287477943 1105 68-69 0.5110877836700389 1105 70-71 0.5676439974595233 1105 72-73 0.5367171699709878 1105 74-75 0.5058025238486579 1105 76-77 0.5047457855164161 1105 78-79 0.3620369720544723 1105 80-81 0.40390723609779755 1105 82-83 0.45683041406121916 1105 84-85 0.37751951765709535 1105 86-87 0.5339212583786939 1105 88-89 0.48329705615436325 1105 90-91 0.3422681250835211 1105 92-93 0.46531318336536387 1105 94-95 0.4006745124193536 1105 96-97 0.447693457215145 1105 98-99 0.3945646795634268 1105 100-101 0.36428557187094057 1105 102-103 -0.03655191952325865 1105 104-105 0.20752761117745067 1105 106-107 0.24386305658061502 1105 108-109 0.30882891780855104 1105 110-111 0.21107549019107097 1105 112-113 0.18850481492787807 1105 114-115 0.1963167223101152 1105 116-117 -0.005895081046286776 1105 118-119 0.13288466599881232 1105 120-121 0.18550723775327427 1105 122-123 0.10944099817629294 1105 124-125 0.570670344593939 1106 1 -0.14883345261816672 1106 2 -0.6227744366551953 1106 3 -0.0669265794873688 1106 4 -0.26625852629667435 1106 5 -0.030599258675323426 1106 6 0.15615377781500683 1106 7 0.27642980356207403 1106 8 0.19529835540789264 1106 9 0.3204434532308085 1106 10-11 0.33097160071486087 1106 12-13 0.3365798814368901 1106 14-15 0.3954957944370534 1106 16-17 0.2605726930055994 1106 18-19 0.3478849151681729 1106 20-21 0.4145800268949458 1106 22-23 0.36385096446461773 1106 24-25 0.33421727343397833 1106 26-27 0.4994383248173264 1106 28-29 0.5054367522295706 1106 30-31 0.5459581051546252 1106 32-33 0.4705775032620636 1106 34-35 0.43030049146286586 1106 36-37 0.40699416255463916 1106 38-39 0.29847590515711175 1106 40-41 0.2744586392595636 1106 42-43 0.2157449224296073 1106 44-45 0.17613271198186453 1106 46-47 0.16928796896045384 1106 48-49 0.21353425051162134 1106 50-51 0.3168999373686958 1106 52-53 0.1585505339648421 1106 54-55 0.1938799068990349 1106 56-57 -0.28402871703409005 1106 58-59 0.1548855489804737 1106 60-61 0.15622287725251027 1106 62-63 0.09226655200916412 1106 64-65 0.22054405179440195 1106 66-67 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120-121 -1.242103852849322 2216 122-123 -1.27138586797631 2216 124-125 -1.6157695469467406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104012.0 3 35262.0 4 17152.0 5 12965.0 6 13733.0 7 15990.0 8 18190.0 9 20005.0 10 21919.0 11 23821.0 12 26141.0 13 28948.0 14 32812.0 15 29208.0 16 21234.0 17 18847.0 18 17668.0 19 16710.0 20 16356.0 21 17169.0 22 19247.0 23 24227.0 24 31316.0 25 41281.0 26 51938.0 27 59365.0 28 68216.0 29 81164.0 30 95528.0 31 112003.0 32 138186.0 33 171218.0 34 222558.0 35 305135.0 36 501640.0 37 795457.0 38 301606.0 39 1031.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8872635772889 19.818780403688375 13.87253367095041 24.421422348072316 2 15.196678735303568 20.628868731047717 39.268282454838946 24.906170078809765 3 18.935113273101596 25.095473326121244 29.35206210483903 26.617351295938125 4 12.861834100174773 17.084009554933207 36.74945075017344 33.304705594718584 5 13.335862665750136 38.42821918941602 34.257342478220636 13.978575666613208 6 31.91914561077711 37.1548070444269 17.249716512649403 13.676330832146588 7 28.123330891801412 32.39882636457786 21.25810203714434 18.219740706476387 8 25.82699075674542 36.17116172578534 19.533501839016623 18.468345678452618 9 26.20222393683233 15.989756164874969 19.771434853804617 38.036585044488085 10-11 24.812976943504047 26.937992952598343 27.528459352767904 20.720570751129706 12-13 25.65080251996312 24.017683037057648 28.07969833885763 22.251816104121602 14-15 22.723278196617844 25.302560099397674 25.75441151573667 26.219750188247808 16-17 22.352391484765462 28.72939612399345 26.343861629563893 22.574350761677195 18-19 22.31092032188598 27.26942366541992 28.58316332313272 21.836492689561375 20-21 23.017986549721105 26.43289077573929 28.655251209155107 21.893871465384496 22-23 22.69380702685947 26.421601934457613 28.388913476997146 22.49567756168577 24-25 22.538179413349773 26.59913216885816 28.44773603970013 22.41495237809194 26-27 22.34074414508659 26.935165975397663 28.285123955233647 22.4389659242821 28-29 22.26509084912466 26.888711451528906 28.510369601768982 22.33582809757745 30-31 22.47017645068737 26.64228571558101 28.45634974830403 22.43118808542759 32-33 22.27849309968271 26.727813608412877 28.3348936235322 22.658799668372218 34-35 22.651956870254313 26.662729106231396 28.27199088306947 22.413323140444817 36-37 22.56550810396973 26.528862440773672 28.20700555187521 22.69862390338139 38-39 22.439291771811526 26.793634809356526 28.37740964248009 22.389663776351856 40-41 22.658190475165036 26.578830451046652 28.348196703103028 22.41478237068528 42-43 22.470247287106808 26.615282872490475 28.091613024607437 22.822856815795276 44-45 22.6282408370258 26.758429108894845 27.9150178309435 22.698312223135854 46-47 22.647550844965146 26.954405146917566 27.532430329548024 22.865613678569265 48-49 22.73024528101941 27.095737970984267 27.371589155567545 22.802427592428774 50-51 22.684990450538237 27.116632072505926 27.277345809237723 22.92103166771812 52-53 22.96054864790276 27.083624943260027 27.145465137431152 22.81036127140606 54-55 22.78461931658156 26.987953275164354 27.286698790510638 22.940728617743446 56-57 22.70031283827055 27.240477418404186 27.231267958446914 22.82794178487835 58-59 22.760589855290313 27.007710020164406 27.25219618906458 22.9795039354807 60-61 22.885037024779713 26.94747734509633 27.38983661721529 22.77764901290866 62-63 22.800160827006696 26.945635598190893 27.369520732119895 22.884682842682512 64-65 22.64712182638134 26.889012175951166 27.401302032271996 23.062563965395498 66-67 22.607004078477686 27.206809476666198 27.167991118813077 23.01819532604304 68-69 22.603618097628452 26.942773806845516 27.37513097653955 23.078477118986484 70-71 22.480702742616153 26.946768980901936 27.405448964059868 23.167079312422047 72-73 22.68721923985155 26.975556901762353 27.131779541195343 23.20544431719075 74-75 22.677624156876835 27.110749431636982 27.07633667491732 23.135289736568858 76-77 22.711686139126126 27.003438116453943 27.145210126321167 23.13966561809876 78-79 22.650370134458857 27.085055838932714 27.069570997643133 23.195003028965296 80-81 22.55953909839084 27.11287774067994 27.164333337630747 23.163249823298475 82-83 22.67537539052118 27.14322670657685 27.055616223013452 23.125781679888522 84-85 22.66055641157433 27.020892210203957 27.011385962715107 23.307165415506603 86-87 22.603518926641236 26.887337224991768 27.252116450540026 23.257027397826967 88-89 22.64335012832842 26.946049927094496 27.074151669516205 23.336448275060878 90-91 22.525361212663004 27.190081766490543 27.12688150411241 23.157675516734045 92-93 22.60679621210157 27.00735707789883 27.163299530925894 23.222547179073704 94-95 22.508346302302964 26.881064926546166 27.214406996372464 23.39618177477841 96-97 22.608953271594775 26.793407249203522 27.094770225961305 23.502869253240398 98-99 22.286411500119293 26.83108038035264 27.07337695861832 23.80913116090975 100-101 22.26236043380437 26.758726389265313 27.299827198529297 23.67908597840102 102-103 22.481141928416683 26.71516222390089 27.15606226577938 23.64763358190305 104-105 22.43196728604143 26.61363946755947 27.257287509159152 23.69710573723995 106-107 22.172188920734275 26.709712243975623 27.359065285241297 23.75903355004881 108-109 22.258435379113028 26.684578838466734 27.018742610874952 24.03824317154529 110-111 22.157453939196113 26.653241710415365 27.259277330468134 23.93002701992039 112-113 22.239873083804017 26.685113414774435 27.284729538050208 23.790283963371337 114-115 22.20412902655459 26.71173374119999 27.061793158788618 24.0223440734568 116-117 21.907310261817074 26.670351104963142 27.204330201985798 24.218008431233986 118-119 22.021626075509356 26.62973350205624 27.035144497795287 24.313495924639117 120-121 21.909981032711347 26.61597389121832 27.18880357607345 24.285241499996882 122-123 21.97105139487461 26.678589386323743 27.081310577307384 24.26904864149427 124-125 21.839554901157836 26.519328869261997 27.114426520349788 24.52668970923037 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 150.0 1 115.0 2 132.0 3 223.0 4 202.5 5 193.0 6 334.0 7 723.0 8 1237.5 9 1513.0 10 1364.5 11 1086.0 12 1121.5 13 1334.5 14 1598.0 15 1865.5 16 2168.0 17 2507.5 18 2916.5 19 3349.5 20 3883.5 21 4703.5 22 5798.0 23 7210.5 24 9066.0 25 12093.5 26 16572.5 27 22050.0 28 26989.0 29 32154.5 30 39115.5 31 45988.5 32 52089.5 33 59161.0 34 68309.0 35 75617.0 36 82599.0 37 91378.5 38 97720.0 39 101080.0 40 103934.0 41 109352.0 42 114567.0 43 120648.5 44 126981.5 45 129051.0 46 128743.5 47 130189.0 48 136559.0 49 140533.5 50 139069.0 51 135723.0 52 132004.0 53 129925.5 54 125620.5 55 115263.0 56 104876.5 57 95802.5 58 87411.5 59 78840.5 60 66521.5 61 52699.5 62 40036.0 63 29396.5 64 21085.0 65 15357.5 66 11143.5 67 8297.5 68 6205.5 69 4499.5 70 3194.5 71 2256.0 72 1627.5 73 1224.5 74 920.5 75 694.5 76 495.0 77 291.5 78 177.0 79 121.0 80 88.0 81 60.0 82 45.0 83 33.0 84 16.5 85 13.0 86 9.0 87 5.0 88 3.0 89 2.0 90 1.0 91 1.0 92 1.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09548749340512935 2 0.0 3 0.0 4 0.10030436992704982 5 0.0 6 0.0 7 0.001501732092128147 8 0.00561024441964855 9 0.0031451370231363076 10-11 3.966839488640388E-4 12-13 0.0 14-15 1.4167283888001386E-5 16-17 1.983419744320194E-4 18-19 0.001643404931008161 20-21 0.001062546291600104 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.250185166400416E-5 52-53 0.0 54-55 0.0 56-57 0.00787700984172877 58-59 5.525240716320541E-4 60-61 0.0 62-63 0.0 64-65 1.7000740665601663E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0012325536982561206 76-77 0.0 78-79 0.0 80-81 4.250185166400416E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.067061688320887E-4 90-91 1.4167283888001386E-5 92-93 0.002025921595984198 94-95 1.4167283888001386E-5 96-97 0.008245359222816807 98-99 0.0031734715909123106 100-101 0.001799245053776176 102-103 0.0 104-105 0.0 106-107 0.001501732092128147 108-109 1.4167283888001386E-5 110-111 2.8334567776002774E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.800296266240665E-4 122-123 0.004009341340304393 124-125 0.0858537403612884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3529258.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.507964959021194 #Duplication Level Percentage of deduplicated Percentage of total 1 72.63207964811504 35.958664541197166 2 13.260617869755537 13.13012409661655 3 5.4347338252437325 8.071878353453217 4 2.7319466564297534 5.410124773457574 5 1.5662848323144032 3.8771787297033935 6 1.0155368071451325 3.0166296397642496 7 0.6531441704216825 2.2635087091697934 8 0.4647099336187536 1.8405474487765057 9 0.3417302343821398 1.5226531622306168 >10 1.7339091039202317 15.180604207379359 >50 0.10886698236648211 3.7040449385742704 >100 0.05408635248250945 4.785904772045774 >500 0.0018943479421632797 0.6316867023357017 >1k 3.4442689694138553E-4 0.2857041613477588 >5k 1.1480896564712852E-4 0.32074576394815535 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6053 0.1715091387481448 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5233 0.14827479317182252 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.8334567776002775E-5 0.0 0.0 0.0 0.0 3 2.8334567776002775E-5 0.0 0.0 0.0 0.0 4 2.8334567776002775E-5 0.0 0.0 0.0 0.0 5 2.8334567776002775E-5 0.0 0.0 0.0 0.0 6 8.500370332800833E-5 0.0 0.0 0.0 0.0 7 1.133382711040111E-4 0.0 0.0 1.4167283888001387E-4 0.0 8 1.133382711040111E-4 0.0 0.0 3.683493810880361E-4 0.0 9 1.133382711040111E-4 0.0 0.0 6.516950588480638E-4 0.0 10-11 1.4167283888001387E-4 0.0 0.0 7.225314782880708E-4 0.0 12-13 1.5584012276801526E-4 0.0 0.0 8.07535181616079E-4 0.0 14-15 2.125092583200208E-4 0.0 0.0 8.358697493920819E-4 0.0 16-17 2.266765422080222E-4 0.0 0.0 9.350407366080916E-4 0.0 18-19 3.116802455360305E-4 0.0 0.0 0.0010342117238241013 0.0 20-21 3.683493810880361E-4 0.0 0.0 0.0012325536982561208 0.0 22-23 3.683493810880361E-4 0.0 0.0 0.0013458919693601318 0.0 24-25 4.816876521920472E-4 0.0 0.0 0.001459230240464143 0.0 26-27 4.816876521920472E-4 0.0 0.0 0.0016717394987841637 0.0 28-29 5.383567877440527E-4 0.0 0.0 0.002408438260960236 0.0 30-31 5.666913555200555E-4 0.0 0.0 0.004547698128048445 0.0 32-33 5.666913555200555E-4 0.0 0.0 0.00888288699777687 0.0 34-35 5.666913555200555E-4 0.0 0.0 0.014790644379073448 0.0 36-37 5.666913555200555E-4 0.0 0.0 0.025359438159522484 0.0 38-39 5.950259232960583E-4 0.0 0.0 0.045363643009380436 0.0 40-41 5.950259232960583E-4 0.0 0.0 0.06896633796679075 0.0 42-43 6.091932071840597E-4 0.0 0.0 0.09163399218759297 0.0 44-45 6.516950588480638E-4 0.0 0.0 0.11890601367199563 0.0 46-47 9.350407366080916E-4 0.0 0.0 0.15226996722823893 0.0 48-49 9.633753043840944E-4 0.0 0.0 0.1888215596592825 0.0 50-51 9.633753043840944E-4 0.0 0.0 0.23138008045883865 0.0 52-53 9.633753043840944E-4 0.0 0.0 0.2723660327468267 0.0 54-55 9.633753043840944E-4 0.0 0.0 0.31568958687633486 0.0 56-57 9.91709872160097E-4 0.0 0.0 0.3686468940496841 0.0 58-59 9.91709872160097E-4 0.0 0.0 0.42892868699313 0.0 60-61 9.91709872160097E-4 0.0 0.0 0.4958974379317126 0.0 62-63 0.0010342117238241013 0.0 0.0 0.5636878913357992 0.0 64-65 0.0010767135754881055 0.0 0.0 0.6355301879318542 0.0 66-67 0.0011617172788161137 0.0 0.0 0.7175162597917182 0.0 68-69 0.001204219130480118 0.0 0.0 0.8121678834474555 0.0 70-71 0.001246720982144122 0.0 0.0 0.9227151996255303 2.8334567776002775E-5 72-73 0.0012892228338081263 0.0 0.0 1.0338575417269014 2.8334567776002775E-5 74-75 0.0013033901176961276 0.0 0.0 1.1497459239307526 2.8334567776002775E-5 76-77 0.0013033901176961276 0.0 0.0 1.2760755943600608 2.8334567776002775E-5 78-79 0.0013033901176961276 0.0 0.0 1.4225794770458833 2.8334567776002775E-5 80-81 0.0015867357954561553 0.0 0.0 1.5858149220034352 2.8334567776002775E-5 82-83 0.0016150703632321582 0.0 0.0 1.7619000934474045 2.8334567776002775E-5 84-85 0.0016150703632321582 0.0 0.0 1.9424762938838702 2.8334567776002775E-5 86-87 0.0016150703632321582 0.0 0.0 2.134216314024081 2.8334567776002775E-5 88-89 0.0016150703632321582 1.4167283888001387E-5 0.0 2.353525868610342 2.8334567776002775E-5 90-91 0.0017425759182241706 2.8334567776002775E-5 0.0 2.6024025446708627 2.8334567776002775E-5 92-93 0.0018134123376641776 2.8334567776002775E-5 0.0 2.868605808926409 2.8334567776002775E-5 94-95 0.0018134123376641776 2.8334567776002775E-5 0.0 3.1415668675965316 2.8334567776002775E-5 96-97 0.0018134123376641776 2.8334567776002775E-5 0.0 3.4209598731518067 2.8334567776002775E-5 98-99 0.0019125833248801872 2.8334567776002775E-5 0.0 3.724933116252765 2.8334567776002775E-5 100-101 0.0020400888798722 2.8334567776002775E-5 0.0 4.068178070291262 2.8334567776002775E-5 102-103 0.002125092583200208 2.8334567776002775E-5 0.0 4.442718554438355 2.8334567776002775E-5 104-105 0.0023517691254082303 2.8334567776002775E-5 0.0 4.8231101268311924 2.8334567776002775E-5 106-107 0.002535943815952248 2.8334567776002775E-5 0.0 5.208290241178174 2.8334567776002775E-5 108-109 0.002564278383728251 2.8334567776002775E-5 0.0 5.616435522707606 2.8334567776002775E-5 110-111 0.0026492820870562595 2.8334567776002775E-5 0.0 6.078628992269763 2.8334567776002775E-5 112-113 0.002663449370944261 2.8334567776002775E-5 0.0 6.570927940093924 2.8334567776002775E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5075 0.0 68.49395 1 GTATCAA 10245 0.0 53.251133 1 TCAACGC 12585 0.0 43.115654 4 ATCAACG 12610 0.0 43.030174 3 CAACGCA 13025 0.0 41.659977 5 TATCAAC 13260 0.0 40.92085 2 AACGCAG 13400 0.0 40.360916 6 ACGCAGA 16080 0.0 33.264084 7 GTACATG 14045 0.0 33.19745 1 CGCAGAG 16250 0.0 32.73302 8 TACATGG 14705 0.0 30.82403 2 ACATGGG 14605 0.0 30.056314 3 GCAGAGT 17950 0.0 28.93647 9 GAGTACT 10190 0.0 26.186897 12-13 AGAGTAC 16730 0.0 24.733097 10-11 CATGGGG 7595 0.0 24.62474 4 GTACTTT 10940 0.0 24.364443 14-15 CAGAGTA 17660 0.0 23.902262 10-11 AGTACTT 11380 0.0 22.690456 12-13 CATGGGA 8785 0.0 22.577314 4 >>END_MODULE