FastQCFastQC Report
Thu 26 May 2016
SRR1364567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3861767
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46950.12157647004596601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50150.054.3366931
GTATCAA96500.051.5439451
ATCAACG116500.042.6946453
CAACGCA120800.040.9286235
TCAACGC121800.040.7391364
AACGCAG123150.039.948666
TATCAAC126900.038.9138222
GTACATG169600.035.2213941
ACGCAGA144500.033.6354067
TACATGG175700.033.6252022
ACATGGG178950.032.4497383
CGCAGAG147100.032.3575748
GCAGAGT160100.028.6524499
CATGGGG97100.026.6540414
CATGGGA96600.025.1906434
GAGTACT61600.024.29788412-13
AGAGTAC146350.023.78674110-11
CAGAGTA153800.022.80863410-11
AGTACAT123450.022.3621882
GTATAGG14550.022.0815431
Produced by FastQC (version 0.11.5)