##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364567_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3861767 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.124408852217133 31.0 28.0 33.0 16.0 34.0 2 28.01188316125753 31.0 28.0 34.0 16.0 34.0 3 28.241799414620303 31.0 28.0 34.0 16.0 34.0 4 31.554559091731843 35.0 32.0 37.0 17.0 37.0 5 31.686580521300222 35.0 32.0 37.0 17.0 37.0 6 31.64536493268496 35.0 32.0 37.0 17.0 37.0 7 31.45139181105437 35.0 32.0 37.0 17.0 37.0 8 31.417630582062564 35.0 32.0 37.0 16.0 37.0 9 32.496125219362014 37.0 32.0 39.0 11.0 39.0 10-11 32.393838882563344 37.0 32.0 39.0 10.5 39.0 12-13 32.315907976840656 37.0 32.0 39.0 10.0 39.0 14-15 32.998812072297476 38.0 32.0 40.0 10.0 41.0 16-17 33.09436056085207 38.0 32.0 40.0 10.0 41.0 18-19 32.86896115172148 38.0 32.0 40.0 10.0 41.0 20-21 32.56993689676254 38.0 31.0 40.0 8.0 41.0 22-23 32.46052817272508 38.0 31.0 40.0 8.0 41.0 24-25 32.26997705972421 38.0 30.0 40.0 8.0 41.0 26-27 32.157914757674405 38.0 30.0 40.0 8.0 41.0 28-29 32.21436262208466 38.0 30.5 40.0 4.5 41.0 30-31 32.1586215584731 38.0 30.0 40.0 2.0 41.0 32-33 32.14630698848481 38.0 30.0 40.0 2.0 41.0 34-35 31.64708901391513 37.0 30.0 40.0 2.0 41.0 36-37 31.508533399348018 37.0 30.0 40.0 2.0 41.0 38-39 31.302520322950606 37.0 30.0 40.0 2.0 41.0 40-41 31.521104976038174 37.0 30.0 40.0 2.0 41.0 42-43 31.400647682783557 37.0 30.0 40.0 2.0 41.0 44-45 31.24800473462019 37.0 30.0 40.0 2.0 41.0 46-47 31.2100016650409 37.0 30.0 40.0 2.0 41.0 48-49 31.058030689060217 37.0 29.5 40.0 2.0 41.0 50-51 30.88650299202412 37.0 29.0 40.0 2.0 41.0 52-53 30.813855677983682 36.5 29.0 40.0 2.0 41.0 54-55 30.830280154136695 36.5 29.0 40.0 2.0 41.0 56-57 30.69265571951907 36.0 29.0 40.0 2.0 41.0 58-59 30.484745843029884 36.0 28.5 40.0 2.0 41.0 60-61 30.269637707298237 35.5 28.0 39.5 2.0 41.0 62-63 29.975776503346786 35.0 28.0 39.0 2.0 41.0 64-65 29.68812139624167 35.0 28.0 39.0 2.0 41.0 66-67 29.434918264100347 35.0 28.0 39.0 2.0 40.0 68-69 29.404558586781647 35.0 28.0 38.0 2.0 40.0 70-71 29.17685789950559 35.0 28.0 37.5 2.0 40.0 72-73 28.892610947268437 34.5 28.0 37.0 2.0 39.5 74-75 28.599802758685335 34.0 28.0 36.5 2.0 39.0 76-77 28.298616798993827 34.0 27.0 36.0 2.0 39.0 78-79 28.001229877411042 34.0 27.0 36.0 2.0 38.5 80-81 27.71510140306238 34.0 26.0 35.0 2.0 37.0 82-83 27.476441872334608 34.0 26.0 35.0 2.0 37.0 84-85 27.24194740386978 34.0 26.0 35.0 2.0 36.0 86-87 27.049159620453537 34.0 26.0 35.0 2.0 36.0 88-89 26.86548269224943 34.0 26.0 35.0 2.0 36.0 90-91 26.735732243814816 34.0 25.0 35.0 2.0 35.0 92-93 26.621252007177027 33.0 25.0 35.0 2.0 35.0 94-95 26.4949014272482 33.0 25.0 35.0 2.0 35.0 96-97 26.38376046509279 33.0 25.0 35.0 2.0 35.0 98-99 26.29025585955859 33.0 24.5 35.0 2.0 35.0 100-101 26.194504614079513 33.0 24.0 35.0 2.0 35.0 102-103 25.044304200641832 31.5 21.5 34.0 2.0 35.0 104-105 25.910799382769596 33.0 23.5 35.0 2.0 35.0 106-107 26.057939409601875 33.0 24.0 35.0 2.0 35.0 108-109 26.001109466210675 33.0 24.0 35.0 2.0 35.0 110-111 25.908678980373494 33.0 23.0 35.0 2.0 35.0 112-113 25.813431260870995 33.0 23.0 35.0 2.0 35.0 114-115 25.68144142305841 33.0 21.5 35.0 2.0 35.0 116-117 25.56102284265208 33.0 19.5 35.0 2.0 35.0 118-119 25.397638179620884 33.0 18.5 35.0 2.0 35.0 120-121 25.202951136099095 33.0 16.5 35.0 2.0 35.0 122-123 24.944062005812363 33.0 2.0 35.0 2.0 35.0 124-125 23.4754485446688 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8572410536499504 1101 2 0.922999545890665 1101 3 0.8709897869048184 1101 4 0.8974330780756148 1101 5 0.8027033714768628 1101 6 0.9967698344425209 1101 7 1.0380512954773984 1101 8 0.8995352949368822 1101 9 1.167009568389986 1101 10-11 1.303872606634748 1101 12-13 1.1489113507579134 1101 14-15 1.430620065711416 1101 16-17 1.2667048902972198 1101 18-19 1.6026583107329344 1101 20-21 1.9488043916775766 1101 22-23 1.7625550771127863 1101 24-25 1.933374514097821 1101 26-27 2.1270864472781383 1101 28-29 1.8376422841076874 1101 30-31 1.916831938205533 1101 32-33 1.8853536687572898 1101 34-35 2.130072872084874 1101 36-37 1.9770356826096247 1101 38-39 1.8899612707695859 1101 40-41 1.6108639171104038 1101 42-43 1.6499013693061393 1101 44-45 1.5891982316545672 1101 46-47 1.4056626415804239 1101 48-49 1.3087982822097501 1101 50-51 1.3346052158202575 1101 52-53 1.1721426966235455 1101 54-55 1.1103294126092287 1101 56-57 1.1801966047998143 1101 58-59 1.1530436822294767 1101 60-61 1.1488008895625086 1101 62-63 1.163295980789247 1101 64-65 1.2052666633916687 1101 66-67 1.1731240252001385 1101 68-69 1.033388038582629 1101 70-71 0.889575256659473 1101 72-73 0.8421235464903205 1101 74-75 0.9074977511243141 1101 76-77 1.0259199175820228 1101 78-79 0.9993656546409895 1101 80-81 1.1333498981332255 1101 82-83 0.9367684315880815 1101 84-85 0.9795569279569243 1101 86-87 0.9283727634673689 1101 88-89 0.8914337774494179 1101 90-91 0.9168729645014437 1101 92-93 0.7929404685249253 1101 94-95 0.8809078455590118 1101 96-97 0.8641989406218329 1101 98-99 0.9574637810870961 1101 100-101 1.0155007484653815 1101 102-103 0.9372171339838822 1101 104-105 0.9682970799506698 1101 106-107 0.9360586844057828 1101 108-109 1.0605159003750302 1101 110-111 0.8806441236570883 1101 112-113 0.9674601077393028 1101 114-115 1.1687677014418831 1101 116-117 1.091733455054527 1101 118-119 1.06520203361994 1101 120-121 1.1924813448568727 1101 122-123 1.2576711263457057 1101 124-125 1.441856050480279 1102 1 0.025438937013980478 1102 2 0.21234353673262518 1102 3 0.3341455642793285 1102 4 0.368460698311992 1102 5 0.5681909098833593 1102 6 -0.26961900810352546 1102 7 -0.7671718637090343 1102 8 0.17443948379872154 1102 9 0.45220684892967 1102 10-11 0.5305673809799103 1102 12-13 0.6325256901661191 1102 14-15 0.8323530203880338 1102 16-17 0.6752436707702003 1102 18-19 0.9073541233934463 1102 20-21 1.309048141227585 1102 22-23 1.0694611001528784 1102 24-25 1.206617988003238 1102 26-27 0.737447289625031 1102 28-29 1.2935229286293648 1102 30-31 1.0192245275963003 1102 32-33 0.8726887889347097 1102 34-35 1.2635435462586244 1102 36-37 1.4110048754276932 1102 38-39 1.3890130951960877 1102 40-41 1.2628766341961075 1102 42-43 1.045329905546037 1102 44-45 1.1221599865609768 1102 46-47 0.8113359006739849 1102 48-49 0.5204932462243406 1102 50-51 0.790427820872484 1102 52-53 0.6533193003253714 1102 54-55 0.5513107123264867 1102 56-57 0.4921977788785412 1102 58-59 0.6177060993442467 1102 60-61 0.5816315198776962 1102 62-63 0.46880408085933567 1102 64-65 0.5450940118018082 1102 66-67 0.5646648840507709 1102 68-69 0.40547938924484583 1102 70-71 0.44641137029292466 1102 72-73 0.6608146857959021 1102 74-75 0.22246784449782808 1102 76-77 0.4286178934722926 1102 78-79 0.3294507170129073 1102 80-81 0.052673787759093926 1102 82-83 0.20361703532783793 1102 84-85 -0.2142385607307311 1102 86-87 0.24574315779136313 1102 88-89 0.43803515083078537 1102 90-91 -0.01000608554920035 1102 92-93 0.3822717440281309 1102 94-95 0.29078437263189016 1102 96-97 0.45399468785886654 1102 98-99 0.5407338080887847 1102 100-101 0.47624766168899413 1102 102-103 0.4167862112782963 1102 104-105 0.5956990498673527 1102 106-107 0.5402405147482234 1102 108-109 0.03050021396448699 1102 110-111 0.2656290960982446 1102 112-113 0.09849638413907158 1102 114-115 0.10907517555294177 1102 116-117 0.13479239308290758 1102 118-119 0.5113783259893765 1102 120-121 0.8033044440478712 1102 122-123 0.6112170805657442 1102 124-125 0.9112882794092876 1103 1 0.5683868015055182 1103 2 0.5125979759074468 1103 3 0.38335075814979547 1103 4 0.5105253511657395 1103 5 0.5240546941764244 1103 6 0.5909724403660555 1103 7 0.6460111781776838 1103 8 0.6622367746540903 1103 9 0.8967032780343871 1103 10-11 0.9951458866331748 1103 12-13 0.9803226124048905 1103 14-15 1.2214404693674439 1103 16-17 1.0882168511005688 1103 18-19 1.1482501522680053 1103 20-21 1.5215818087316535 1103 22-23 1.5237735239695098 1103 24-25 1.618061706182722 1103 26-27 1.5371640796116495 1103 28-29 1.480671454256651 1103 30-31 1.4419508816219349 1103 32-33 1.4338640417650907 1103 34-35 1.8146194537895894 1103 36-37 1.6866169039498686 1103 38-39 1.7188163882544067 1103 40-41 1.4435788988140992 1103 42-43 1.4135594962129154 1103 44-45 0.9553191589604673 1103 46-47 1.034103927119407 1103 48-49 1.0448259564498272 1103 50-51 0.9749169494940624 1103 52-53 1.0720172371344887 1103 54-55 0.9032443563390586 1103 56-57 0.8528302593746595 1103 58-59 0.8848124246177882 1103 60-61 0.7578994829085772 1103 62-63 0.6965922125291897 1103 64-65 0.8753475637976784 1103 66-67 0.9628147879293039 1103 68-69 1.0087876451947224 1103 70-71 0.9967296145032982 1103 72-73 0.9802215847993239 1103 74-75 0.9910128678924863 1103 76-77 1.0080615666680544 1103 78-79 1.0247986036214414 1103 80-81 0.9792823150482803 1103 82-83 1.0037474881202364 1103 84-85 0.971211017203256 1103 86-87 0.926073619455547 1103 88-89 0.9443968174526773 1103 90-91 0.98064278276658 1103 92-93 1.0034984688570852 1103 94-95 0.9751101541147165 1103 96-97 1.1181041757379404 1103 98-99 1.0583057929767499 1103 100-101 1.031129613782582 1103 102-103 0.9452642495382015 1103 104-105 0.9304846337091988 1103 106-107 0.986681365215464 1103 108-109 1.105445280718456 1103 110-111 1.17212191838318 1103 112-113 1.1377850727948662 1103 114-115 1.161703193289025 1103 116-117 1.1926827721269895 1103 118-119 1.1859892792756597 1103 120-121 1.2098959337525343 1103 122-123 1.1677474205239697 1103 124-125 1.2658426469225397 1104 1 0.7815894758738899 1104 2 0.8333152285336425 1104 3 0.7050879034687725 1104 4 0.7963500514934267 1104 5 0.7390696558415435 1104 6 0.8148121210902097 1104 7 0.8535223906462086 1104 8 0.8776237877414559 1104 9 1.1465194181355116 1104 10-11 1.1740522802279898 1104 12-13 1.050314244760017 1104 14-15 1.1693879964196086 1104 16-17 1.2089952440210538 1104 18-19 1.4801379766569198 1104 20-21 1.7933325196448848 1104 22-23 1.552279813019986 1104 24-25 1.9931101003792833 1104 26-27 2.0067148228364644 1104 28-29 1.874374267354085 1104 30-31 1.7251982507454144 1104 32-33 1.717921556372616 1104 34-35 1.9886610672377643 1104 36-37 2.102700211039803 1104 38-39 2.1039615942582763 1104 40-41 1.8230575936605256 1104 42-43 1.8360531538951683 1104 44-45 1.805894255280581 1104 46-47 1.6459419788015417 1104 48-49 1.5787890679844736 1104 50-51 1.4223786175441155 1104 52-53 1.5455868644859976 1104 54-55 1.391651173145295 1104 56-57 1.4064237370018198 1104 58-59 1.363254266120137 1104 60-61 1.351341798548198 1104 62-63 1.426376784453474 1104 64-65 1.3857163313802658 1104 66-67 1.325324856029468 1104 68-69 1.3407471169940912 1104 70-71 1.3218760995070973 1104 72-73 1.274198973134144 1104 74-75 1.2613028847858203 1104 76-77 1.2147765650934517 1104 78-79 1.2787986100603383 1104 80-81 1.249793522833965 1104 82-83 1.1767010589592708 1104 84-85 1.2073682282458549 1104 86-87 1.2904739587271088 1104 88-89 1.2813350672188264 1104 90-91 1.3261379957361612 1104 92-93 1.3324041746601765 1104 94-95 1.311784262590301 1104 96-97 1.1420691516271795 1104 98-99 1.2641698174305311 1104 100-101 1.2759221670176615 1104 102-103 1.2774399074475689 1104 104-105 1.3370629957229632 1104 106-107 1.3415595646177003 1104 108-109 1.4801114822232861 1104 110-111 1.4899749135844438 1104 112-113 1.4861256215999816 1104 114-115 1.3946689629191873 1104 116-117 1.545184519410654 1104 118-119 1.5416166741079138 1104 120-121 1.506384171635986 1104 122-123 1.5386250017916865 1104 124-125 1.8059648455614727 1105 1 0.17983068833644822 1105 2 0.5742748813843122 1105 3 0.5794254780316344 1105 4 0.6356731615008471 1105 5 0.6606314438142 1105 6 0.6054767226109128 1105 7 0.7286292747505563 1105 8 0.48776241706481827 1105 9 0.5210720409562271 1105 10-11 0.6800288297274975 1105 12-13 0.674023635200605 1105 14-15 0.8157248157534696 1105 16-17 0.8398316281382066 1105 18-19 0.9786976013465107 1105 20-21 1.312523976596836 1105 22-23 1.4420352596291295 1105 24-25 1.4388401764142031 1105 26-27 1.3026804279156963 1105 28-29 0.9663931608795906 1105 30-31 0.8961472151856427 1105 32-33 1.0711882418133314 1105 34-35 1.6287096451031502 1105 36-37 1.633679640649902 1105 38-39 1.751886314567539 1105 40-41 1.2740125103293174 1105 42-43 0.8919196191674352 1105 44-45 1.1268137785902788 1105 46-47 1.0970956231014846 1105 48-49 1.0370879466614156 1105 50-51 0.9945349883487573 1105 52-53 0.9185541464296278 1105 54-55 0.804616791025019 1105 56-57 0.8420292642540694 1105 58-59 0.8169545682117345 1105 60-61 0.8726299760142133 1105 62-63 0.8416151296059269 1105 64-65 0.8897926479108236 1105 66-67 0.7352602775496493 1105 68-69 0.6211582047798601 1105 70-71 0.7736681683225406 1105 72-73 0.8318853482909461 1105 74-75 0.8522513372063933 1105 76-77 0.868087732242266 1105 78-79 0.9185258955881075 1105 80-81 0.8270502494922667 1105 82-83 0.7805424864700861 1105 84-85 0.7639982428384151 1105 86-87 0.8184078313878196 1105 88-89 0.8881455687420896 1105 90-91 0.699082556807312 1105 92-93 0.709847468482657 1105 94-95 0.7334836558469995 1105 96-97 0.8614484557924627 1105 98-99 0.74905371697227 1105 100-101 0.7043542988115199 1105 102-103 0.7468313720798143 1105 104-105 0.8990180147956934 1105 106-107 0.8789043586577883 1105 108-109 0.8714212285175407 1105 110-111 0.9653230869542995 1105 112-113 0.9838324135407923 1105 114-115 0.9954689936403653 1105 116-117 0.9976396536878092 1105 118-119 1.0092205500967495 1105 120-121 0.8793536936656317 1105 122-123 0.9820474692144323 1105 124-125 1.042658734606814 1106 1 0.1754739779525849 1106 2 0.4233171740630226 1106 3 0.1270684407056457 1106 4 0.3535078839939665 1106 5 0.21620953975401136 1106 6 0.3159242939886049 1106 7 0.011875585807661793 1106 8 0.2417735808991175 1106 9 0.30498156060801307 1106 10-11 0.569069437532967 1106 12-13 0.518335860209973 1106 14-15 0.7737724312742458 1106 16-17 0.5388617342557112 1106 18-19 0.5065365315965948 1106 20-21 0.9489882184820786 1106 22-23 0.9944790192393285 1106 24-25 1.0867672807131612 1106 26-27 1.3635177479463039 1106 28-29 1.165086366119283 1106 30-31 0.9496324979212432 1106 32-33 0.8066039673165051 1106 34-35 1.1945517468098927 1106 36-37 1.0231127560513187 1106 38-39 1.3060511972652442 1106 40-41 1.0109191125832702 1106 42-43 0.8768713406151072 1106 44-45 0.7210641413101406 1106 46-47 0.4917375491801046 1106 48-49 0.5244183638755686 1106 50-51 0.5668204023333239 1106 52-53 0.5967356429806543 1106 54-55 0.39530659760098175 1106 56-57 0.4771577203465931 1106 58-59 0.399904196993738 1106 60-61 0.47528326775783114 1106 62-63 0.40107086834021644 1106 64-65 0.4192657133975075 1106 66-67 0.28897256080664135 1106 68-69 0.31174243383527767 1106 70-71 0.453535452835073 1106 72-73 0.4084666283755993 1106 74-75 0.4175463272314133 1106 76-77 0.30550643563346824 1106 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2216 88-89 -2.7752416868401397 2216 90-91 -2.751466792540924 2216 92-93 -2.751226448795407 2216 94-95 -2.8314031698405344 2216 96-97 -2.763016750898217 2216 98-99 -2.844997423371286 2216 100-101 -2.8130446772044415 2216 102-103 -2.674146702402961 2216 104-105 -2.8485023084790235 2216 106-107 -2.7848291467179855 2216 108-109 -2.9057131561317817 2216 110-111 -2.959469024903054 2216 112-113 -2.995984265358448 2216 114-115 -2.993275744303997 2216 116-117 -3.0797322702945316 2216 118-119 -3.1422317937489836 2216 120-121 -3.202985410413028 2216 122-123 -3.216753062439338 2216 124-125 -3.2510745196059396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 269734.0 3 45952.0 4 29128.0 5 22902.0 6 21498.0 7 22917.0 8 23619.0 9 24595.0 10 25962.0 11 26606.0 12 28363.0 13 30122.0 14 33092.0 15 29342.0 16 23156.0 17 20989.0 18 19682.0 19 18802.0 20 18897.0 21 20145.0 22 24388.0 23 32551.0 24 42609.0 25 53701.0 26 65781.0 27 75356.0 28 86704.0 29 101423.0 30 114843.0 31 131931.0 32 160510.0 33 200404.0 34 258144.0 35 352155.0 36 535301.0 37 668700.0 38 201432.0 39 331.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86253109731368 18.755610206600632 13.147138788159115 24.23471990792657 2 15.224594944962858 20.311717449931038 39.97429545568724 24.489392149418865 3 18.52634517578514 25.46169868445689 29.022436407906156 26.989519731851818 4 12.324538107566301 16.59490176102196 37.30825536825406 33.772304763157685 5 13.722813982061425 38.03978373428199 33.939841126123234 14.297561157533353 6 31.801728968719672 36.485932189871676 17.743836690555824 13.968502150852832 7 28.596311857496236 32.396640023866645 21.274747142010682 17.732300976626433 8 26.130732382352427 35.31186630368948 19.995043719623684 18.562357594334408 9 26.984458282437835 16.138324575035004 19.635282239217165 37.24193490330999 10-11 24.864925822816343 26.670666562741875 27.573426879456996 20.890980734984787 12-13 25.50675979689858 24.113224574935245 27.718708546353955 22.66130708181222 14-15 22.828115052714622 25.482313030532676 25.36085416700121 26.32871774975149 16-17 22.753038733925298 28.812516828969493 25.67064293365899 22.763801503446224 18-19 22.540439250422047 27.51825505569938 27.763144844288522 22.178160849590046 20-21 23.411073983232633 26.765453929102414 27.63070355560079 22.192768532064168 22-23 23.281169521770444 26.50975054645891 27.379980376421564 22.82909955534908 24-25 22.95218696342414 26.709700644085267 27.552022769592543 22.786089622898047 26-27 22.755322455322453 27.097876197876197 27.435638435638438 22.711162911162912 28-29 22.736177067772072 26.79423049583958 27.749586780925657 22.72000565546269 30-31 22.842281923512065 26.68656632934606 27.549604358901387 22.921547388240484 32-33 22.828795332689957 26.982316435924126 27.25263216640655 22.936256064979364 34-35 22.99622855139133 26.698906427519063 27.464111933010493 22.84075308807911 36-37 23.334661466530978 26.45261924743406 27.309258685427835 22.903460600607126 38-39 22.847248422807485 26.645781581333104 27.519125312324643 22.987844683534767 40-41 22.994364718603087 26.713368113137147 27.38933509429671 22.902932073963054 42-43 23.067078056509303 26.706021315684385 26.998973424540747 23.227927203265565 44-45 22.94887678258391 26.83942691067653 26.981226853101862 23.2304694536377 46-47 23.002572639283965 26.984809529007226 26.699331895845724 23.31328593586309 48-49 23.230844225812067 26.810183880512895 26.72296030219894 23.2360115914761 50-51 23.144697981015618 26.916908686695525 26.51858882145849 23.41980451083037 52-53 23.302959500145917 26.865318389224413 26.447154890494428 23.38456722013524 54-55 23.304267041762376 26.693881134683515 26.511664286922322 23.49018753663179 56-57 23.058567675292117 27.08112271791656 26.40951860192815 23.45079100486317 58-59 23.193340851504043 26.673908624446657 26.581347081831964 23.551403442217335 60-61 23.450969414239157 26.762032528357203 26.61030724563971 23.17669081176393 62-63 23.178714116444443 26.819349705927642 26.697415843942267 23.304520333685645 64-65 23.125731660571653 26.619352616366402 26.61327918613445 23.64163653692749 66-67 23.191701649255002 26.94944147409303 26.3686325366427 23.490224340009267 68-69 22.83039715466338 26.805764127354298 26.82420535655547 23.539633361426855 70-71 23.013568300373315 26.654267815191858 26.861226087929296 23.470937796505535 72-73 23.150445888179107 26.766162119213256 26.47814896710471 23.60524302550293 74-75 23.02737322060083 27.04276306675157 26.273892236377804 23.6559714762698 76-77 23.188562302611295 26.756672872785696 26.54918935240773 23.50557547219528 78-79 23.10680906407819 26.83582671226802 26.566387711199198 23.490976512454594 80-81 23.05792049504599 27.27142306300903 26.397178801337574 23.273477640607403 82-83 23.27917544140182 26.964272882232642 26.34967349245572 23.406878183909818 84-85 23.190666345224866 26.735183660743907 26.298919639636466 23.775230354394765 86-87 23.175802839679378 26.488403876445265 26.7111525167178 23.62464076715756 88-89 23.177135031068335 26.652232901861407 26.59748145955328 23.57315060751698 90-91 23.10577658012429 26.838782734415933 26.66247591548358 23.392964769976192 92-93 23.10263500167354 26.716188806503048 26.46076177515766 23.72041441666575 94-95 23.13245812648198 26.50833183643598 26.53684951176108 23.822360525320963 96-97 23.19138201552845 26.599451650616597 26.477230757914572 23.73193557594038 98-99 22.96403128079497 26.620941052204444 26.439714009043353 23.97531365795723 100-101 22.91238174656408 26.591206321007093 26.67278273998358 23.823629192445246 102-103 23.052076937837004 26.262886911613258 26.763680978163624 23.921355172386114 104-105 22.931950527130127 26.451244104785115 26.797826093993272 23.818979274091483 106-107 22.950833893094508 26.443368137789808 26.83832992470284 23.76746804441284 108-109 23.034183574513946 26.449446587533632 26.430802272638402 24.08556756531401 110-111 22.726779544796578 26.580593337832166 26.829444505153887 23.863182612217376 112-113 22.79912278498418 26.491953036006578 26.86697566165955 23.8419485173497 114-115 22.69356229933085 26.68654012528462 26.63522941700004 23.98466815838449 116-117 22.50920368836338 26.721058002722586 26.888261254498264 23.881477054415765 118-119 22.726720695474377 26.55483875645527 26.62506567589396 24.09337487217639 120-121 22.693865967021306 26.547362010023395 26.827091543091942 23.931680479863356 122-123 22.73464452930485 26.566219557005898 26.86114931325479 23.837986600434462 124-125 22.86381721855368 26.67451385770856 26.437492253931445 24.024176669806323 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1182.0 1 1273.5 2 1594.5 3 1580.0 4 1152.0 5 859.0 6 776.5 7 962.0 8 1316.5 9 1518.5 10 1464.5 11 1406.5 12 1514.0 13 1737.0 14 2014.0 15 2249.5 16 2418.0 17 2690.0 18 2987.0 19 3265.5 20 3739.5 21 4549.5 22 5620.5 23 6966.0 24 8754.0 25 11327.5 26 15209.0 27 19575.0 28 24154.5 29 29500.5 30 36265.5 31 45105.5 32 54084.5 33 62321.5 34 70453.0 35 78499.5 36 88880.0 37 98276.5 38 102682.5 39 105988.5 40 109258.5 41 114369.0 42 120699.5 43 128079.0 44 133441.0 45 135429.0 46 135353.0 47 134150.5 48 138271.0 49 141868.0 50 140632.0 51 138766.5 52 139675.5 53 142266.5 54 141035.0 55 133104.0 56 121789.5 57 111134.5 58 103929.5 59 96277.5 60 84123.5 61 69469.0 62 55727.5 63 43836.0 64 33888.5 65 26334.0 66 19860.5 67 16419.5 68 12932.5 69 8784.5 70 6380.0 71 4695.5 72 3642.0 73 2936.5 74 2207.5 75 1674.0 76 1171.5 77 777.5 78 522.5 79 356.5 80 286.5 81 207.0 82 146.5 83 103.5 84 88.5 85 78.0 86 57.0 87 38.5 88 29.5 89 25.0 90 18.5 91 19.0 92 19.0 93 12.0 94 8.5 95 6.5 96 6.5 97 4.5 98 5.5 99 7.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012973335781262826 2 0.0049977121871930645 3 0.008286362175656894 4 0.008182782648461184 5 0.015303875143166326 6 0.0011134799173538952 7 0.008053308239466544 8 0.0 9 0.0024859086526970685 10-11 0.0 12-13 0.003871284828939706 14-15 0.020431061739354033 16-17 0.03201902134437422 18-19 0.006059402340949104 20-21 0.008752470048037597 22-23 0.0022269598347077904 24-25 0.01235185861808856 26-27 0.01986137433977762 28-29 3.6252834518498914E-4 30-31 0.0015536929079356676 32-33 0.00923152536131776 34-35 0.009995424374386129 36-37 0.011846908423009467 38-39 0.0 40-41 0.025933724121626187 42-43 0.009969529492587201 44-45 0.017958100527556427 46-47 0.004363287583119334 48-49 0.02586898691712887 50-51 0.004453919669415581 52-53 0.0 54-55 0.018333576313640878 56-57 0.004958869864494673 58-59 3.1073858158713356E-4 60-61 0.004725815928304323 62-63 0.027642786320355425 64-65 0.017789783795863396 66-67 0.002175170071109935 68-69 0.02208833417448541 70-71 0.003081490934072408 72-73 0.01255901767247998 74-75 0.0 76-77 0.006616142299626052 78-79 0.001022847831057648 80-81 0.0037806527426434582 82-83 0.0016702198760308426 84-85 0.0 86-87 0.0062018241908432074 88-89 0.017647361945969293 90-91 0.02289107551025217 92-93 0.005036554509891457 94-95 0.025985513885224045 96-97 0.018773789304222653 98-99 9.451631856608646E-4 100-101 0.009244472802217223 102-103 0.0 104-105 0.001411271058041565 106-107 2.5894881798927798E-5 108-109 0.0 110-111 5.437925177774837E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.5894881798927798E-5 122-123 0.0 124-125 0.024276451686494806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3861767.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.79491474750631 #Duplication Level Percentage of deduplicated Percentage of total 1 73.00726629253572 30.513324706450216 2 10.894291474129805 9.1065196679148 3 5.006977976103749 6.277986529616937 4 2.842046354668116 4.7513234040726005 5 1.8432241503652576 3.851869811253035 6 1.2649476199055778 3.172102676040879 7 0.9047001308649539 2.6468305389090987 8 0.6668800519544945 2.229775593460054 9 0.5244898520318209 1.972890778644194 >10 2.7571709944536926 20.463262747434953 >50 0.18095111460920216 5.221611390757327 >100 0.10138138488734344 7.720530535600796 >500 0.004525335376559662 1.20072061039086 >1k 0.0011472681136478415 0.8712510094544059 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4695 0.12157647004596601 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.1789763597855596E-5 2 0.0 0.0 0.0 2.5894881798927798E-5 5.1789763597855596E-5 3 0.0 0.0 0.0 2.5894881798927798E-5 5.1789763597855596E-5 4 0.0 0.0 0.0 2.5894881798927798E-5 5.1789763597855596E-5 5 0.0 0.0 0.0 2.5894881798927798E-5 5.1789763597855596E-5 6 0.0 0.0 0.0 2.5894881798927798E-5 1.0357952719571119E-4 7 0.0 0.0 0.0 2.5894881798927798E-5 1.0357952719571119E-4 8 0.0 0.0 0.0 2.5894881798927798E-5 1.0357952719571119E-4 9 0.0 0.0 0.0 2.5894881798927798E-5 1.0357952719571119E-4 10-11 0.0 0.0 0.0 3.8842322698391695E-5 1.5536929079356678E-4 12-13 0.0 2.5894881798927798E-5 0.0 7.768464539678339E-5 4.143181087828447E-4 14-15 0.0 2.5894881798927798E-5 0.0 7.768464539678339E-5 4.6610787238070034E-4 16-17 0.0 2.5894881798927798E-5 0.0 1.0357952719571119E-4 5.567399586769476E-4 18-19 0.0 2.5894881798927798E-5 0.0 1.29474408994639E-4 6.085297222748032E-4 20-21 0.0 2.5894881798927798E-5 0.0 1.6831673169303066E-4 6.732669267721228E-4 22-23 0.0 2.5894881798927798E-5 0.0 2.977911406876697E-4 6.732669267721228E-4 24-25 2.5894881798927798E-5 2.5894881798927798E-5 0.0 6.473720449731949E-4 6.732669267721228E-4 26-27 2.5894881798927798E-5 2.5894881798927798E-5 0.0 0.0011134799173538952 7.768464539678339E-4 28-29 5.1789763597855596E-5 2.5894881798927798E-5 0.0 0.0019680110167185124 7.768464539678339E-4 30-31 6.47372044973195E-5 2.5894881798927798E-5 0.0 0.004971817305394137 7.768464539678339E-4 32-33 7.768464539678339E-5 2.5894881798927798E-5 0.0 0.011251326141634127 8.027413357667617E-4 34-35 7.768464539678339E-5 2.5894881798927798E-5 0.0 0.0205864310301476 8.545310993646173E-4 36-37 7.768464539678339E-5 2.5894881798927798E-5 0.0 0.036226939636699984 8.545310993646173E-4 38-39 1.0357952719571119E-4 2.5894881798927798E-5 0.0 0.062082979112929385 9.063208629624729E-4 40-41 1.0357952719571119E-4 2.5894881798927798E-5 0.0 0.09236704337677545 9.322157447614007E-4 42-43 1.0357952719571119E-4 2.5894881798927798E-5 0.0 0.12266405508152096 9.581106265603284E-4 44-45 1.29474408994639E-4 2.5894881798927798E-5 0.0 0.16045763506705607 9.840055083592562E-4 46-47 1.5536929079356678E-4 2.5894881798927798E-5 0.0 0.20462135597512743 0.001022847831057648 48-49 1.9421161349195848E-4 2.5894881798927798E-5 0.0 0.252669309153038 0.001035795271957112 50-51 2.0715905439142238E-4 2.5894881798927798E-5 0.0 0.30447202019179304 0.0010616901537560397 52-53 2.0715905439142238E-4 2.5894881798927798E-5 0.0 0.35644304796224113 0.0011134799173538952 54-55 2.0715905439142238E-4 2.5894881798927798E-5 0.0 0.4162213825950659 0.001139374799152823 56-57 2.0715905439142238E-4 2.5894881798927798E-5 0.0 0.48718630616502756 0.001152322240052287 58-59 2.0715905439142238E-4 2.5894881798927798E-5 0.0 0.5660880110063606 0.0011652696809517507 60-61 2.201064952908863E-4 2.5894881798927798E-5 0.0 0.6502981666164738 0.0011782171218512147 62-63 2.3305393619035017E-4 2.5894881798927798E-5 0.0 0.7345989543128832 0.0012429543263485342 64-65 2.3305393619035017E-4 2.5894881798927798E-5 0.0 0.8259302024177015 0.0012947440899463898 66-67 2.3305393619035017E-4 2.5894881798927798E-5 0.0 0.9360999770312399 0.0013206389717453175 68-69 2.3305393619035017E-4 2.5894881798927798E-5 0.0 1.061029834270167 0.0013465338535442455 70-71 2.58948817989278E-4 2.5894881798927798E-5 0.0 1.1959292210016814 0.0013594812944437095 72-73 2.58948817989278E-4 2.5894881798927798E-5 0.0 1.3341431526034584 0.001398323617142101 74-75 2.58948817989278E-4 2.5894881798927798E-5 0.0 1.4830516703881929 0.0014630608216394205 76-77 2.58948817989278E-4 2.5894881798927798E-5 0.0 1.6497758668505895 0.0015019031443378123 78-79 2.58948817989278E-4 2.5894881798927798E-5 0.0 1.8402327224817032 0.0015019031443378123 80-81 2.58948817989278E-4 2.5894881798927798E-5 0.0 2.0455143979427035 0.00152779802613674 82-83 2.8484369978820575E-4 2.5894881798927798E-5 0.0 2.2629407729674007 0.001657272435131379 84-85 2.8484369978820575E-4 2.5894881798927798E-5 0.0 2.4887700371358497 0.001657272435131379 86-87 2.8484369978820575E-4 2.5894881798927798E-5 0.0 2.7307836024286294 0.0017220096396286986 88-89 2.8484369978820575E-4 2.5894881798927798E-5 0.0 3.0045054504841953 0.0017867468441260179 90-91 2.8484369978820575E-4 2.5894881798927798E-5 0.0 3.304044495693293 0.0017996942850254819 92-93 2.8484369978820575E-4 2.5894881798927798E-5 0.0 3.632145595526607 0.0018903263713217291 94-95 2.8484369978820575E-4 2.5894881798927798E-5 0.0 3.9704233839069003 0.0019162212531206569 96-97 2.8484369978820575E-4 2.5894881798927798E-5 0.0 4.320470396064806 0.002136327748411543 98-99 2.8484369978820575E-4 2.5894881798927798E-5 0.0 4.703196749052959 0.002201064952908863 100-101 2.8484369978820575E-4 2.5894881798927798E-5 0.0 5.126720488315323 0.002201064952908863 102-103 2.8484369978820575E-4 2.5894881798927798E-5 0.0 5.580709556014125 0.002239907275607254 104-105 3.1073858158713356E-4 2.5894881798927798E-5 0.0 6.037637692797105 0.002278749598305646 106-107 3.2368602248659744E-4 2.5894881798927798E-5 0.0 6.504535359072673 0.002278749598305646 108-109 3.366334633860614E-4 2.5894881798927798E-5 0.0 7.0083202844708135 0.002278749598305646 110-111 3.4958090428552526E-4 2.5894881798927798E-5 0.0 7.548060253246765 0.002278749598305646 112-113 3.8842322698391695E-4 2.5894881798927798E-5 0.0 8.118187865813757 0.002278749598305646 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5015 0.0 54.336693 1 GTATCAA 9650 0.0 51.543945 1 ATCAACG 11650 0.0 42.694645 3 CAACGCA 12080 0.0 40.928623 5 TCAACGC 12180 0.0 40.739136 4 AACGCAG 12315 0.0 39.94866 6 TATCAAC 12690 0.0 38.913822 2 GTACATG 16960 0.0 35.221394 1 ACGCAGA 14450 0.0 33.635406 7 TACATGG 17570 0.0 33.625202 2 ACATGGG 17895 0.0 32.449738 3 CGCAGAG 14710 0.0 32.357574 8 GCAGAGT 16010 0.0 28.652449 9 CATGGGG 9710 0.0 26.654041 4 CATGGGA 9660 0.0 25.190643 4 GAGTACT 6160 0.0 24.297884 12-13 AGAGTAC 14635 0.0 23.786741 10-11 CAGAGTA 15380 0.0 22.808634 10-11 AGTACAT 12345 0.0 22.362188 2 GTATAGG 1455 0.0 22.081543 1 >>END_MODULE