FastQCFastQC Report
Thu 26 May 2016
SRR1364567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3861767
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85000.2201064952908863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76140.1971636300170362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46800.1211880468189821No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67250.057.8875771
GTATCAA118800.051.03511
ATCAACG152650.039.2893373
TCAACGC157750.037.8303534
CAACGCA158100.037.7084855
TATCAAC160700.037.358272
AACGCAG160750.037.1238636
GTACATG159600.031.2275431
ACGCAGA190750.031.0357157
CGCAGAG192300.030.5999168
TACATGG163800.029.4518892
ACATGGG165000.028.9850233
GCAGAGT211950.026.9208269
GAGTACT112350.025.57829712-13
AGAGTAC192700.024.0357510-11
CAGAGTA201450.023.47905210-11
GTACTTT123200.023.30151714-15
AGTACTT121850.023.1446412-13
CATGGGG90500.022.9019644
CGTATAG3255.9480953E-1022.0121421
Produced by FastQC (version 0.11.5)