##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364567_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3861767 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.057134467201156 31.0 30.0 34.0 26.0 34.0 2 29.940523081791316 31.0 30.0 34.0 26.0 34.0 3 30.244314066591794 31.0 30.0 34.0 26.0 34.0 4 33.81478090211036 35.0 35.0 37.0 30.0 37.0 5 33.221034826803375 35.0 33.0 37.0 27.0 37.0 6 33.22651573748494 36.0 33.0 37.0 27.0 37.0 7 33.01788455906325 36.0 33.0 37.0 26.0 37.0 8 32.94450493776554 36.0 33.0 37.0 26.0 37.0 9 34.197904223636485 38.0 34.0 39.0 25.0 39.0 10-11 34.13062582491383 38.0 34.0 39.0 25.0 39.0 12-13 34.016116456534014 38.0 34.0 39.0 25.0 39.0 14-15 34.99531626325462 39.0 34.0 41.0 25.0 41.0 16-17 34.981230094928044 39.0 34.0 41.0 25.0 41.0 18-19 34.98540732778544 39.0 34.0 41.0 25.0 41.0 20-21 34.98222083310567 39.0 34.0 41.0 25.0 41.0 22-23 34.931989164545655 39.0 34.0 41.0 24.0 41.0 24-25 34.887049115081254 39.0 34.0 41.0 24.0 41.0 26-27 34.76298324057355 39.0 34.0 41.0 24.0 41.0 28-29 34.614312567278134 39.0 34.0 41.0 23.5 41.0 30-31 34.47081543241734 38.5 33.5 41.0 23.0 41.0 32-33 34.28496462888621 38.0 33.0 41.0 22.0 41.0 34-35 34.10496684548809 38.0 33.0 40.0 20.5 41.0 36-37 33.91770956145205 38.0 33.0 40.0 18.5 41.0 38-39 33.74904182463624 38.0 33.0 40.0 18.0 41.0 40-41 33.78860816822973 38.0 33.0 40.0 17.5 41.0 42-43 33.66435572109866 38.0 33.0 40.0 15.5 41.0 44-45 33.51298809586389 38.0 33.0 40.0 15.0 41.0 46-47 33.29064078697653 38.0 32.0 40.0 11.0 41.0 48-49 33.0266027183929 38.0 32.0 40.0 8.5 41.0 50-51 32.85447231798294 38.0 32.0 40.0 7.5 41.0 52-53 32.666561188181475 38.0 31.5 40.0 6.5 41.0 54-55 32.46846003396891 37.0 31.0 40.0 2.0 41.0 56-57 32.241269605338694 37.0 31.0 40.0 2.0 41.0 58-59 32.026961103557 37.0 31.0 40.0 2.0 41.0 60-61 31.77769036816566 36.0 31.0 40.0 2.0 41.0 62-63 31.59046933696414 36.0 30.5 39.5 2.0 41.0 64-65 31.44555678786421 36.0 30.5 39.0 2.0 41.0 66-67 31.136073590146687 35.0 30.0 39.0 2.0 41.0 68-69 30.84916063035393 35.0 30.0 39.0 2.0 40.5 70-71 30.53577882870717 35.0 30.0 38.0 2.0 40.0 72-73 30.242111965843613 35.0 30.0 37.0 2.0 39.5 74-75 29.894409217334967 35.0 29.0 37.0 2.0 39.0 76-77 29.589949756160845 35.0 29.0 36.0 2.0 39.0 78-79 29.312844223900612 34.0 29.0 36.0 2.0 38.5 80-81 29.043418077786672 34.0 29.0 35.5 2.0 37.0 82-83 28.768278096529386 34.0 29.0 35.0 2.0 37.0 84-85 28.55615123853925 34.0 29.0 35.0 2.0 36.0 86-87 28.347975939511628 34.0 29.0 35.0 2.0 36.0 88-89 28.18849842572066 34.0 28.5 35.0 2.0 36.0 90-91 28.05415085892028 34.0 28.0 35.0 2.0 35.5 92-93 27.952810721102544 34.0 28.0 35.0 2.0 35.0 94-95 27.84766105774895 34.0 28.0 35.0 2.0 35.0 96-97 27.7350036913154 34.0 27.0 35.0 2.0 35.0 98-99 27.65537796557897 34.0 27.0 35.0 2.0 35.0 100-101 27.554477652328586 34.0 27.0 35.0 2.0 35.0 102-103 26.262588861523753 31.5 24.5 34.0 2.0 35.0 104-105 27.222528961483178 33.5 26.5 35.0 2.0 35.0 106-107 27.391537215994646 34.0 27.0 35.0 2.0 35.0 108-109 27.392436415765115 34.0 27.0 35.0 2.0 35.0 110-111 27.312902357910254 34.0 27.0 35.0 2.0 35.0 112-113 27.23495371419353 34.0 27.0 35.0 2.0 35.0 114-115 27.125378486066094 34.0 27.0 35.0 2.0 35.0 116-117 26.99657307134273 34.0 26.0 35.0 2.0 35.0 118-119 26.855293444684776 34.0 25.0 35.0 2.0 35.0 120-121 26.653134691968727 34.0 25.0 35.0 2.0 35.0 122-123 26.35480519668846 34.0 25.0 35.0 2.0 35.0 124-125 24.502286518062846 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7450716570985101 1101 2 0.8930989741896163 1101 3 0.7303058981800525 1101 4 0.640699894763415 1101 5 0.7403679216593915 1101 6 0.833253971664206 1101 7 0.8707319781671004 1101 8 0.899458292447818 1101 9 1.0705591050459944 1101 10-11 1.0610265743389604 1101 12-13 0.9526754308690712 1101 14-15 1.1499425664774279 1101 16-17 1.1587548570537223 1101 18-19 1.228756434213885 1101 20-21 1.2213094529192219 1101 22-23 1.2125903223420025 1101 24-25 1.1980416223798898 1101 26-27 1.2396424993023558 1101 28-29 1.3692837330421668 1101 30-31 1.3968256262667325 1101 32-33 1.3744501409130478 1101 34-35 1.3719296991644327 1101 36-37 1.4177236117248526 1101 38-39 1.4672543286898687 1101 40-41 1.2435148036631674 1101 42-43 1.3149274874918788 1101 44-45 1.2786432361021696 1101 46-47 1.3744835681789809 1101 48-49 1.4357497294589905 1101 50-51 1.4496846436504782 1101 52-53 1.458269828892412 1101 54-55 1.3937489658922573 1101 56-57 1.4008139621813243 1101 58-59 1.3837771358037045 1101 60-61 1.328965691762697 1101 62-63 1.4049793358237679 1101 64-65 1.3228133125515313 1101 66-67 1.3935059210607825 1101 68-69 1.4633307242545008 1101 70-71 1.4348411460190178 1101 72-73 1.371542504598363 1101 74-75 1.4347169072906425 1101 76-77 1.3974733477429702 1101 78-79 1.318365086132868 1101 80-81 1.08735251258652 1101 82-83 1.3324857437398698 1101 84-85 1.3111400249935699 1101 86-87 1.3627227870764607 1101 88-89 1.3760506746488552 1101 90-91 1.2934980037121946 1101 92-93 1.203296450392358 1101 94-95 1.1366973779127427 1101 96-97 1.2518388312668236 1101 98-99 1.3453955816713545 1101 100-101 1.3083682199047288 1101 102-103 1.2237775245332898 1101 104-105 1.2579038409485754 1101 106-107 1.248482608695948 1101 108-109 1.2434006681363279 1101 110-111 1.1496392166557783 1101 112-113 1.1157782821088027 1101 114-115 1.2643595698648937 1101 116-117 1.2450604601454849 1101 118-119 1.2378697793328222 1101 120-121 1.2050918763483836 1101 122-123 1.1297903904880826 1101 124-125 1.526777893737016 1102 1 0.3852202705837797 1102 2 -0.24112502765317245 1102 3 0.33495588134065457 1102 4 0.4052874080511941 1102 5 0.5542290883784489 1102 6 0.41417025719307077 1102 7 0.48712777109949457 1102 8 0.49148655493982574 1102 9 0.8436755347485771 1102 10-11 0.8791623378451447 1102 12-13 0.9011163231988562 1102 14-15 1.0917127100883803 1102 16-17 1.0855683962815448 1102 18-19 1.1488807725077805 1102 20-21 0.9990011116944331 1102 22-23 1.1087892190648816 1102 24-25 1.0440399031263325 1102 26-27 1.1107147823220913 1102 28-29 0.7756068906937728 1102 30-31 1.0074032626437344 1102 32-33 1.100933362093926 1102 34-35 1.1806229042446361 1102 36-37 0.8375017721135691 1102 38-39 0.2775928692809302 1102 40-41 -0.02820585556788302 1102 42-43 0.315180780408987 1102 44-45 0.04090151208441739 1102 46-47 0.19002745837136814 1102 48-49 -0.07484826830565794 1102 50-51 -0.1377252930061701 1102 52-53 -0.4373418880863511 1102 54-55 -0.33771845906946396 1102 56-57 -0.3861172494581915 1102 58-59 -0.2592248483631394 1102 60-61 -0.3756838952134025 1102 62-63 -0.3091143849576312 1102 64-65 -0.31993132326632434 1102 66-67 -0.4250880438576097 1102 68-69 -0.2605649598051478 1102 70-71 -0.6627435416933842 1102 72-73 -0.5389135748871041 1102 74-75 -0.3670731104322229 1102 76-77 -0.71857360005788 1102 78-79 -0.5178502294739467 1102 80-81 -0.6247426913599057 1102 82-83 -0.8470140043866117 1102 84-85 -0.6559983424742306 1102 86-87 -0.49955001983020253 1102 88-89 -0.5904041341762145 1102 90-91 -0.5207811844477419 1102 92-93 -0.38045926137149877 1102 94-95 -0.17518990092115772 1102 96-97 -0.49698359609508813 1102 98-99 -0.606561416899762 1102 100-101 -0.42388425868165314 1102 102-103 -0.4827130257488861 1102 104-105 -0.3389532242938387 1102 106-107 -0.20845240132346632 1102 108-109 -0.17280387686730236 1102 110-111 -0.12403286960687865 1102 112-113 0.036565137593051134 1102 114-115 0.05647371704562687 1102 116-117 -0.1777853693045124 1102 118-119 0.0948141386825192 1102 120-121 0.3414355120592951 1102 122-123 0.3180945904306043 1102 124-125 0.468630502995655 1103 1 0.30478569034750436 1103 2 -0.09071764382519731 1103 3 0.24367497611823197 1103 4 0.3229681035994929 1103 5 0.40971713364206863 1103 6 0.4007533624318569 1103 7 0.5598328671928456 1103 8 0.5752045407437123 1103 9 0.7703643142464998 1103 10-11 0.6969694095360737 1103 12-13 0.7142787163078523 1103 14-15 0.8123141611882687 1103 16-17 0.7006648238867967 1103 18-19 0.8505881546488752 1103 20-21 0.8393383740226241 1103 22-23 0.8563264696473212 1103 24-25 0.7937321082596043 1103 26-27 0.8523842500584493 1103 28-29 0.8694960239386802 1103 30-31 0.8539055752443616 1103 32-33 0.9195989043758104 1103 34-35 0.8877351117645347 1103 36-37 0.9059880199026438 1103 38-39 0.8609560928394089 1103 40-41 0.8491308925713881 1103 42-43 0.8392805749208492 1103 44-45 0.8828958934926447 1103 46-47 0.9257556854691842 1103 48-49 0.878362569772321 1103 50-51 0.809344392401421 1103 52-53 0.8545956857541128 1103 54-55 0.9319063142157091 1103 56-57 0.9694650585690923 1103 58-59 0.9705686513641467 1103 60-61 0.9874725156854041 1103 62-63 0.7884323621882459 1103 64-65 0.7879076368849667 1103 66-67 0.7953751603953485 1103 68-69 0.8670582003137817 1103 70-71 0.6953929982712168 1103 72-73 0.7598070911968549 1103 74-75 0.8311337884462482 1103 76-77 0.8291284658820111 1103 78-79 0.6917332243886882 1103 80-81 0.6412316685436039 1103 82-83 0.7292763567889331 1103 84-85 0.885343915546148 1103 86-87 0.8467214388107038 1103 88-89 0.831464858232156 1103 90-91 0.895552904653762 1103 92-93 0.7959502754273728 1103 94-95 0.7822619805542352 1103 96-97 0.8547872630752522 1103 98-99 0.8646530779799058 1103 100-101 0.8546219725009365 1103 102-103 0.6482017869532655 1103 104-105 0.681290824890862 1103 106-107 0.8041022585839777 1103 108-109 0.853362654119298 1103 110-111 0.7355493190885873 1103 112-113 0.6867744212335332 1103 114-115 0.7895144947891808 1103 116-117 0.8402457775492245 1103 118-119 0.6758945164708088 1103 120-121 0.759791916210542 1103 122-123 0.8148770516524522 1103 124-125 1.121592884108992 1104 1 0.49048205166586456 1104 2 0.6319305767551704 1104 3 0.3755407180025827 1104 4 0.1930189006769325 1104 5 0.4855189231883372 1104 6 0.5325992022577495 1104 7 0.658160157714903 1104 8 0.4954915284266832 1104 9 0.6883397949104761 1104 10-11 0.67546110862164 1104 12-13 0.6901422168692051 1104 14-15 0.7553904252909263 1104 16-17 0.7105855902799547 1104 18-19 0.7034982453437237 1104 20-21 0.5200730936318863 1104 22-23 0.6954825257851738 1104 24-25 0.8707126703873911 1104 26-27 0.7223537560074078 1104 28-29 0.8843487984612395 1104 30-31 0.7662947090716585 1104 32-33 0.8865717310417622 1104 34-35 0.9450175968994543 1104 36-37 1.0000192608662317 1104 38-39 1.0366623964066761 1104 40-41 0.9510557874709562 1104 42-43 1.0352520470761135 1104 44-45 0.9468524900299258 1104 46-47 0.9945896033767028 1104 48-49 0.9134771909951667 1104 50-51 0.8215951191192303 1104 52-53 0.8914368983514152 1104 54-55 0.5634756764015592 1104 56-57 0.6711671967525987 1104 58-59 0.8252313963497002 1104 60-61 0.8906733081385454 1104 62-63 0.8767768094554 1104 64-65 0.8781345115710764 1104 66-67 0.8451914175674098 1104 68-69 0.5941425604567492 1104 70-71 0.26933672578628176 1104 72-73 0.26358000929520387 1104 74-75 0.7157033420430778 1104 76-77 0.8584945677594753 1104 78-79 0.8594807240462536 1104 80-81 0.8274652391016701 1104 82-83 0.8783675058752571 1104 84-85 0.8067286919933494 1104 86-87 0.8061417708706955 1104 88-89 0.8447128390512582 1104 90-91 0.8699384285988678 1104 92-93 0.7839254956085284 1104 94-95 0.8196951915874671 1104 96-97 0.8111160298617577 1104 98-99 0.8155228125683962 1104 100-101 0.783123007993332 1104 102-103 0.7502448356910634 1104 104-105 0.7733684739426501 1104 106-107 0.8233135107395491 1104 108-109 0.8706402603231211 1104 110-111 0.82921093785178 1104 112-113 0.8539099764951104 1104 114-115 0.9152167301415588 1104 116-117 0.8774553245666645 1104 118-119 0.8864586169036173 1104 120-121 0.8621100670049273 1104 122-123 0.9272128011347043 1104 124-125 1.3147248739753294 1105 1 0.19548640383298732 1105 2 0.013014650383599502 1105 3 0.192756739097792 1105 4 0.31143112770644876 1105 5 -0.008446925100237479 1105 6 0.37884161796651483 1105 7 0.4508741179309723 1105 8 0.6206496144971823 1105 9 0.6839377058702567 1105 10-11 0.6696823267164049 1105 12-13 0.6799533456165463 1105 14-15 0.757511786751202 1105 16-17 0.6342063373166411 1105 18-19 0.7026329321156908 1105 20-21 0.7401474644877268 1105 22-23 0.7503438324593574 1105 24-25 0.7017356216687176 1105 26-27 0.7551412550473771 1105 28-29 0.7429068870918414 1105 30-31 0.7828351842259025 1105 32-33 0.8384252422165943 1105 34-35 0.898205559317212 1105 36-37 0.9381183238702562 1105 38-39 0.8440543602580917 1105 40-41 0.7069212927063617 1105 42-43 0.7497601241862739 1105 44-45 0.7196511254975206 1105 46-47 0.6408688204986461 1105 48-49 0.8272930815253687 1105 50-51 0.6201464234893237 1105 52-53 0.6312409868284163 1105 54-55 0.5762470468221892 1105 56-57 0.524141710973943 1105 58-59 0.37105555599743667 1105 60-61 0.5377077542612767 1105 62-63 0.6433374453838212 1105 64-65 0.45677593140159445 1105 66-67 0.5982020931773526 1105 68-69 0.6515335642712934 1105 70-71 0.7925331365284514 1105 72-73 0.7258560006932733 1105 74-75 0.7086048294601923 1105 76-77 0.7284678147321593 1105 78-79 0.6476201085986197 1105 80-81 0.5939305113681286 1105 82-83 0.609665347712383 1105 84-85 0.5612834268951694 1105 86-87 0.6760193341691263 1105 88-89 0.5866664500699166 1105 90-91 0.4286926847623036 1105 92-93 0.520308012697182 1105 94-95 0.4901007751618742 1105 96-97 0.5001594491290575 1105 98-99 0.3630923192374489 1105 100-101 0.38713980223823086 1105 102-103 0.15205706528067964 1105 104-105 0.36500623283258804 1105 106-107 0.35887007611166055 1105 108-109 0.46704556228671734 1105 110-111 0.3619907565968177 1105 112-113 0.2552527480575364 1105 114-115 0.24085259455875274 1105 116-117 0.031066663242068415 1105 118-119 0.22116650968670015 1105 120-121 0.35996662469674234 1105 122-123 0.3511865217255732 1105 124-125 0.7735140114782091 1106 1 -0.21264011450778852 1106 2 -0.6344218194204316 1106 3 -0.04054689010734691 1106 4 -0.22609527773896332 1106 5 -0.016014041922147726 1106 6 0.21639112771561742 1106 7 0.23075699711080944 1106 8 0.16168315291687207 1106 9 0.26014039110377496 1106 10-11 0.18295399349273822 1106 12-13 0.26774702920089766 1106 14-15 0.28347013585870684 1106 16-17 0.1663739311553769 1106 18-19 0.18436996855055554 1106 20-21 0.27395490324730787 1106 22-23 0.21081607203357322 1106 24-25 0.2354950961299025 1106 26-27 0.3038222589721542 1106 28-29 0.4166279989665398 1106 30-31 0.3398674496958307 1106 32-33 0.23802379387477401 1106 34-35 0.37096368204385044 1106 36-37 0.3195401768826187 1106 38-39 0.30933205893961 1106 40-41 0.2608584224436612 1106 42-43 0.13083017507770478 1106 44-45 0.19025424814891778 1106 46-47 0.0849553728201542 1106 48-49 0.2262319654783198 1106 50-51 0.3107872920222903 1106 52-53 0.21286979007564355 1106 54-55 0.2760124628958778 1106 56-57 -0.12751330075673195 1106 58-59 0.2888483484579396 1106 60-61 0.24909147779570873 1106 62-63 0.12833425415720257 1106 64-65 0.3838356741736817 1106 66-67 0.12214598601351057 1106 68-69 0.20636342600841573 1106 70-71 0.27049012593440835 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-1.3704579939937531 2216 78-79 -1.386916862218012 2216 80-81 -1.3524134429476007 2216 82-83 -1.2407233841502894 2216 84-85 -1.2362098646419284 2216 86-87 -1.3553247457281152 2216 88-89 -1.3193149466146785 2216 90-91 -1.3425397239951167 2216 92-93 -1.3610598135223597 2216 94-95 -1.2930634501917382 2216 96-97 -1.3524827097769645 2216 98-99 -1.3690902392410358 2216 100-101 -1.421568095925565 2216 102-103 -1.301458747715003 2216 104-105 -1.2575231285055075 2216 106-107 -1.297453726811117 2216 108-109 -1.3106235332194167 2216 110-111 -1.376011274525574 2216 112-113 -1.3460593884696053 2216 114-115 -1.5010474105913545 2216 116-117 -1.54045129757802 2216 118-119 -1.5866663644073036 2216 120-121 -1.7223835026479826 2216 122-123 -1.6212442010429626 2216 124-125 -1.9861242046757326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126317.0 3 41813.0 4 20180.0 5 15684.0 6 16697.0 7 19579.0 8 22058.0 9 23697.0 10 25777.0 11 27654.0 12 29807.0 13 32834.0 14 37227.0 15 32511.0 16 24052.0 17 21063.0 18 20324.0 19 19259.0 20 19147.0 21 19668.0 22 22418.0 23 27772.0 24 36477.0 25 47276.0 26 58774.0 27 66667.0 28 77091.0 29 91784.0 30 107037.0 31 125770.0 32 154124.0 33 191825.0 34 248090.0 35 343662.0 36 562284.0 37 827169.0 38 277696.0 39 503.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.614539120607674 19.674546818886967 14.051251877589582 24.65966218291578 2 15.460258337765676 20.844608399369616 38.49210438954613 25.20302887331858 3 18.22652687228411 25.277418342432362 29.736724147262123 26.759330638021407 4 12.980696576013404 17.194068245402253 36.46151329781926 33.363721880765084 5 13.515108498259993 38.336284918277045 33.81708425184637 14.33152233161659 6 31.920387739602102 36.886352801709684 17.229081920271213 13.964177538417 7 28.212145184951165 32.61806020413316 21.199835823388437 17.969958787527236 8 26.087069115091687 35.57307595693935 19.620772077389013 18.71908285057995 9 26.715397421863894 15.700066319196546 19.575557142106163 38.008979116833395 10-11 25.04163266870391 26.56243243026285 27.355932580842214 21.04000232019102 12-13 25.646044932280997 23.960495286225193 27.84519366393674 22.548266117557066 14-15 22.964427655340927 25.212480617665918 25.52022857947819 26.30286314751497 16-17 22.642541566868676 28.49329431312333 26.039386968556688 22.8247771514513 18-19 22.516350328974628 26.923740242629147 28.365049967682594 22.194859460713634 20-21 23.294462876089284 26.26517589589706 28.23137434239079 22.20898688562287 22-23 23.08497638516254 25.99484899011256 28.12703355743627 22.793141067288627 24-25 22.843920930496324 26.22054877987201 28.226003795671772 22.70952649395989 26-27 22.632049007617496 26.67335963044896 28.119187408251197 22.575403953682343 28-29 22.577242490290068 26.294737616225937 28.499557326995646 22.62846256648835 30-31 22.71067881619994 26.143783402779093 28.40596027673342 22.739577504287546 32-33 22.735926325953894 26.380579149389387 28.115212543895062 22.768281980761657 34-35 22.86019586370695 26.30253197564742 28.06331920077001 22.773952959875622 36-37 23.182457667694607 26.020614397502488 27.936434279955265 22.860493654847637 38-39 22.78321038011874 26.209854193689054 28.10002519571999 22.906910230472217 40-41 22.760409936694785 26.186160376843038 28.21182634788686 22.841603338575318 42-43 22.939084621107384 26.21671633736577 27.74914954734452 23.095049494182327 44-45 22.818530998892474 26.332971409202056 27.671529639152233 23.176967952753238 46-47 22.94307243290442 26.470330809704468 27.330325729128663 23.25627102826245 48-49 23.154633617201657 26.402123691046093 27.26318030062404 23.180062391128207 50-51 23.089941234140028 26.567339916504523 26.967273522010398 23.375445327345055 52-53 23.289791952751163 26.503502153288892 26.81224682897751 23.394459064982428 54-55 23.178638172629267 26.404855601075884 26.90239985996048 23.514106366334374 56-57 22.999763948806283 26.744328329471013 26.796899248093432 23.459008473629275 58-59 23.086934505309202 26.392503587436806 27.026958440112693 23.493603467141305 60-61 23.358089703495835 26.443115288933793 27.10327940551566 23.095515602054707 62-63 23.082723530446035 26.523816688060155 27.116705901728405 23.2767538797654 64-65 23.0058404739706 26.285939423710726 27.092372555145538 23.615847547173132 66-67 22.98138391052593 26.65947997380474 26.869357990785048 23.489778124884282 68-69 22.68881352614147 26.507208553391305 27.218541048507205 23.585436871960024 70-71 22.910082353492587 26.362297362839342 27.208075992155923 23.51954429151215 72-73 23.03819728119278 26.49880223224239 26.770802070658327 23.6921984159065 74-75 22.95711027921557 26.747070192254537 26.550396655177426 23.74542287335247 76-77 23.059586453558694 26.549750412181783 26.750552273091564 23.64011086116796 78-79 22.948419725995898 26.627590426869357 26.79153092353837 23.632458923596374 80-81 22.930378828221873 26.96962152135935 26.611119505376234 23.488880145042543 82-83 23.126744829504215 26.697636082135457 26.56476944362516 23.61084964473517 84-85 23.050341980756478 26.48523331417975 26.498426756456304 23.965997948607466 86-87 23.04177077488103 26.267793991714157 26.863609327025685 23.826825906379128 88-89 22.99445389978177 26.382851175867767 26.760571221779916 23.86212370257055 90-91 22.89475258346762 26.605929358244556 26.83864666097152 23.660671397316307 92-93 22.907424419278385 26.46236452634312 26.608518437235585 24.021692617142918 94-95 22.915691562397676 26.21837700440977 26.73021530431734 24.135716128875217 96-97 22.980330409752376 26.335341878986153 26.64701498866673 24.03731272259475 98-99 22.745207969712194 26.346364997073767 26.58232814207509 24.326098891138955 100-101 22.637470231555863 26.409946029987196 26.754795805920295 24.197787932536645 102-103 22.837395420282995 26.029897712627402 26.75289575989437 24.37981110719523 104-105 22.74818755248569 26.16387783105506 26.835448643069356 24.252485973389902 106-107 22.700937277867475 26.269804995966815 26.82914197448543 24.200115751680276 108-109 22.82884307335038 26.19302930317373 26.431689543074334 24.546438080401558 110-111 22.518734980941332 26.309377071594298 26.73631970914816 24.435568238316208 112-113 22.53348014004988 26.259002679343418 26.77337861139732 24.43413856920938 114-115 22.40831722887476 26.455842623337972 26.539483091548504 24.596357056238762 116-117 22.26634853941214 26.409076466809108 26.744764767009503 24.57981022676925 118-119 22.401908245629524 26.28977874636145 26.536828866164115 24.771484141844912 120-121 22.34351063201693 26.29954642113953 26.651603477403402 24.705339469440137 122-123 22.371041502037443 26.292983846126255 26.620634576791918 24.71534007504438 124-125 22.36796645071996 26.401461301304625 26.294935079914104 24.935637168061316 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 570.0 1 413.5 2 408.0 3 568.0 4 452.0 5 381.5 6 664.0 7 1590.0 8 2745.0 9 3278.5 10 2686.0 11 1811.0 12 1696.0 13 1850.5 14 2075.0 15 2331.5 16 2621.0 17 3081.0 18 3566.0 19 3988.0 20 4493.0 21 5285.0 22 6452.5 23 7786.5 24 9419.5 25 11872.0 26 15519.5 27 19855.0 28 24594.5 29 30071.0 30 36822.0 31 45453.5 32 53765.5 33 61423.5 34 69478.0 35 77536.5 36 87949.5 37 97472.0 38 101546.5 39 105059.0 40 109015.5 41 113810.5 42 119702.0 43 127248.5 44 132896.5 45 134920.0 46 135102.0 47 134148.0 48 138610.0 49 142132.0 50 140906.0 51 139280.5 52 140186.0 53 143473.5 54 142529.0 55 134606.5 56 122980.0 57 111719.5 58 104664.0 59 97142.5 60 84714.0 61 69547.0 62 55520.5 63 43281.5 64 33086.5 65 25299.5 66 18869.5 67 15855.0 68 12199.0 69 7855.5 70 5645.5 71 4188.5 72 3276.5 73 2601.5 74 2014.0 75 1660.0 76 1110.5 77 589.0 78 329.5 79 209.5 80 151.0 81 113.0 82 80.5 83 53.0 84 28.0 85 21.0 86 14.5 87 11.5 88 9.5 89 5.0 90 2.0 91 2.0 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09741654532756637 2 2.5894881798927798E-5 3 0.0 4 0.10238836263296051 5 0.0 6 0.0 7 0.0018644314895228014 8 0.006137086986345889 9 0.0036252834518498912 10-11 4.1431810878284477E-4 12-13 0.0 14-15 7.768464539678339E-5 16-17 1.9421161349195845E-4 18-19 0.0018385366077238734 20-21 0.0013206389717453175 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.5894881798927798E-5 52-53 0.0 54-55 0.0 56-57 0.008195730089360647 58-59 6.862143676715866E-4 60-61 0.0 62-63 0.0 64-65 2.201064952908863E-4 66-67 0.0 68-69 1.2947440899463899E-5 70-71 0.0 72-73 0.0 74-75 9.710580674597924E-4 76-77 0.0 78-79 0.0 80-81 9.063208629624728E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.001152322240052287 90-91 0.0 92-93 0.002188117512009399 94-95 1.2947440899463899E-5 96-97 0.009050261188725265 98-99 0.0038065476244423862 100-101 0.0022140123938083262 102-103 0.0 104-105 0.0 106-107 0.0013465338535442453 108-109 2.5894881798927798E-5 110-111 1.8126417259249457E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.063208629624728E-4 122-123 0.004803500573701106 124-125 0.09223756896778082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3861767.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.67672949097868 #Duplication Level Percentage of deduplicated Percentage of total 1 69.16533635272756 25.367583315615427 2 12.033519660578957 8.82700290830856 3 5.701248773074225 6.273094770324522 4 3.2371518681777482 4.749125735614861 5 2.146919400981069 3.9370991054358315 6 1.469896879293612 3.234660613289133 7 1.046854730073797 2.6876645425887706 8 0.7985178499370104 2.3429618540686104 9 0.6199110991471066 2.046268052268632 >10 3.4025043502727876 22.299846860294643 >50 0.2323064799716387 5.908689023885278 >100 0.1366163982161071 9.152737388120679 >500 0.007656500125680861 1.8470111825914202 >1k 0.0014178703841915157 0.907267696953006 >5k 1.4178703841915158E-4 0.41898695064055086 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8500 0.2201064952908863 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7614 0.1971636300170362 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4680 0.1211880468189821 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0357952719571119E-4 2 0.0 0.0 0.0 0.0 1.0357952719571119E-4 3 0.0 0.0 0.0 0.0 1.0357952719571119E-4 4 0.0 0.0 0.0 0.0 1.0357952719571119E-4 5 0.0 0.0 0.0 0.0 1.0357952719571119E-4 6 0.0 0.0 0.0 0.0 1.5536929079356678E-4 7 0.0 0.0 0.0 0.0 1.5536929079356678E-4 8 0.0 0.0 0.0 0.0 1.5536929079356678E-4 9 0.0 0.0 0.0 0.0 1.5536929079356678E-4 10-11 0.0 0.0 0.0 0.0 1.5536929079356678E-4 12-13 0.0 0.0 0.0 2.5894881798927798E-5 3.7547578608445307E-4 14-15 0.0 0.0 0.0 2.5894881798927798E-5 4.2726554968230865E-4 16-17 0.0 0.0 0.0 5.1789763597855596E-5 4.6610787238070034E-4 18-19 1.2947440899463899E-5 0.0 0.0 1.0357952719571119E-4 4.6610787238070034E-4 20-21 2.5894881798927798E-5 0.0 0.0 1.424218498941029E-4 4.6610787238070034E-4 22-23 2.5894881798927798E-5 0.0 0.0 2.977911406876697E-4 4.6610787238070034E-4 24-25 5.1789763597855596E-5 0.0 0.0 6.991618085710505E-4 4.6610787238070034E-4 26-27 5.1789763597855596E-5 0.0 0.0 0.0011782171218512147 5.178976359785559E-4 28-29 5.1789763597855596E-5 0.0 0.0 0.0021233803075120794 5.437925177774837E-4 30-31 5.1789763597855596E-5 0.0 0.0 0.005166028918886095 5.437925177774837E-4 32-33 5.1789763597855596E-5 0.0 0.0 0.01175627633671322 5.437925177774837E-4 34-35 9.06320862962473E-5 0.0 0.0 0.021518646774909 5.696873995764115E-4 36-37 1.0357952719571119E-4 0.0 0.0 0.037819474867334044 5.696873995764115E-4 38-39 1.29474408994639E-4 0.0 0.0 0.06459478264742538 6.214771631742671E-4 40-41 1.29474408994639E-4 0.0 0.0 0.09538379710635053 6.214771631742671E-4 42-43 1.424218498941029E-4 0.0 0.0 0.12601744227448214 6.214771631742671E-4 44-45 1.5536929079356678E-4 0.0 0.0 0.1636038632056258 6.34424604073731E-4 46-47 1.5536929079356678E-4 0.0 0.0 0.20776758411369717 6.473720449731949E-4 48-49 1.5536929079356678E-4 0.0 0.0 0.25551774615092004 7.250566903699783E-4 50-51 1.5536929079356678E-4 0.0 0.0 0.30672487490829975 7.768464539678339E-4 52-53 1.5536929079356678E-4 0.0 0.0 0.35742705347060033 8.027413357667617E-4 54-55 1.5536929079356678E-4 0.0 0.0 0.4166098058220499 8.545310993646173E-4 56-57 1.8126417259249457E-4 0.0 0.0 0.4873416754558212 9.063208629624729E-4 58-59 1.8126417259249457E-4 0.0 0.0 0.5654535864022868 9.322157447614007E-4 60-61 1.8126417259249457E-4 0.0 0.0 0.6490681597310247 9.451631856608646E-4 62-63 1.8126417259249457E-4 0.0 0.0 0.7336926334499208 0.001035795271957112 64-65 1.8126417259249457E-4 0.0 0.0 0.8242988248643691 0.0010746375946555035 66-67 2.0715905439142238E-4 0.0 0.0 0.9338212274329343 0.0011134799173538952 68-69 2.0715905439142238E-4 0.0 0.0 1.0573657084956187 0.0011134799173538952 70-71 2.201064952908863E-4 0.0 0.0 1.1909315088144883 0.001126427358253359 72-73 2.3305393619035017E-4 0.0 0.0 1.3276694321537266 0.0011911645627506787 74-75 2.3305393619035017E-4 0.0 0.0 1.4752055212031177 0.001255901767247998 76-77 2.3305393619035017E-4 0.0 0.0 1.6400134964123936 0.001268849208147462 78-79 2.4600137708981405E-4 0.0 0.0 1.8292273977171591 0.0013724287353431733 80-81 2.848436997882058E-4 0.0 0.0 2.0341206499511753 0.001398323617142101 82-83 3.1073858158713356E-4 0.0 0.0 2.2500451218315347 0.0014242184989410288 84-85 3.1073858158713356E-4 0.0 0.0 2.474786800964429 0.0014242184989410288 86-87 3.2368602248659744E-4 0.0 0.0 2.7167226816118113 0.0015019031443378123 88-89 3.366334633860614E-4 0.0 0.0 2.989253365104627 0.0016054826715335233 90-91 3.6252834518498914E-4 0.0 0.0 3.287860194568963 0.001631377553332451 92-93 3.6252834518498914E-4 0.0 0.0 3.6139544410628606 0.00176085196232709 94-95 3.6252834518498914E-4 0.0 0.0 3.951662542043578 0.0018514840486233376 96-97 3.6252834518498914E-4 0.0 1.2947440899463899E-5 4.300388915229738 0.0020974854257131514 98-99 3.6252834518498914E-4 0.0 2.5894881798927798E-5 4.681522732987257 0.0022528547165067184 100-101 3.8842322698391695E-4 0.0 2.5894881798927798E-5 5.10384236024597 0.0022528547165067184 102-103 4.2726554968230865E-4 0.0 2.5894881798927798E-5 5.556575526177524 0.0022787495983056464 104-105 4.402129905817725E-4 0.0 2.5894881798927798E-5 6.014047455478282 0.002304644480104574 106-107 4.6610787238070034E-4 0.0 2.5894881798927798E-5 6.481450071948929 0.002304644480104574 108-109 4.6610787238070034E-4 0.0 2.5894881798927798E-5 6.986322582382624 0.0023305393619035015 110-111 4.6610787238070034E-4 0.0 2.5894881798927798E-5 7.527991719852595 0.0023305393619035015 112-113 4.6610787238070034E-4 0.0 2.5894881798927798E-5 8.100074395995408 0.0023305393619035015 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6725 0.0 57.887577 1 GTATCAA 11880 0.0 51.0351 1 ATCAACG 15265 0.0 39.289337 3 TCAACGC 15775 0.0 37.830353 4 CAACGCA 15810 0.0 37.708485 5 TATCAAC 16070 0.0 37.35827 2 AACGCAG 16075 0.0 37.123863 6 GTACATG 15960 0.0 31.227543 1 ACGCAGA 19075 0.0 31.035715 7 CGCAGAG 19230 0.0 30.599916 8 TACATGG 16380 0.0 29.451889 2 ACATGGG 16500 0.0 28.985023 3 GCAGAGT 21195 0.0 26.920826 9 GAGTACT 11235 0.0 25.578297 12-13 AGAGTAC 19270 0.0 24.03575 10-11 CAGAGTA 20145 0.0 23.479052 10-11 GTACTTT 12320 0.0 23.301517 14-15 AGTACTT 12185 0.0 23.14464 12-13 CATGGGG 9050 0.0 22.901964 4 CGTATAG 325 5.9480953E-10 22.012142 1 >>END_MODULE