FastQCFastQC Report
Thu 26 May 2016
SRR1364566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156194
Sequences flagged as poor quality0
Sequence length125
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48840.2265102305265667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37470.17377842624550482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89050.050.909611
GGTATCA51200.050.19871
ATCAACG106200.041.847043
TCAACGC106500.041.6732984
CAACGCA107550.041.487715
AACGCAG111450.039.979766
TATCAAC112400.039.80342
GTACATG141200.035.8569681
TACATGG142650.034.7826732
ACGCAGA128750.034.238087
CGCAGAG128700.033.6528248
ACATGGG144250.032.9945953
GCAGAGT138600.029.8332319
CATGGGA96300.029.7157974
TATCACG1454.0725918E-628.7209422
TACCTGG18350.028.2066692
GAGTACT54500.027.95329512-13
CATGGGG51750.026.5564634
GTACTTT60600.024.79580914-15
AGTACTT57700.024.5981312-13
Produced by FastQC (version 0.11.5)