FastQCFastQC Report
Thu 26 May 2016
SRR1364566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156194
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93420.4332634262037646No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82890.38442737527328247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50940.23624961390301616No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24760.1148319678099466No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67400.058.556281
GTATCAA112150.047.5240671
ATCAACG144700.036.59193
TCAACGC145700.036.2181174
TATCAAC146200.036.0535162
CAACGCA147950.035.6309245
AACGCAG151700.034.632476
GTACATG132700.032.5269281
TACATGG135300.031.0783982
ACGCAGA174050.029.5015687
ACATGGG137850.029.380143
CGCAGAG175100.029.222728
GAGTACT104950.026.78660212-13
GCAGAGT187650.026.7286649
GTACTTT110800.025.69451714-15
GTGGTAT36150.025.2323951
TGGTATC35250.025.006582
CATGGGA93950.024.8508364
AGTACTT105200.024.71518912-13
CATGGGG49700.024.5668334
Produced by FastQC (version 0.11.5)