##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364566_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2156194 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.915037793445304 31.0 30.0 34.0 26.0 34.0 2 29.76901104446075 31.0 30.0 34.0 26.0 34.0 3 30.075006701623323 31.0 30.0 34.0 26.0 34.0 4 33.61509817762224 35.0 35.0 37.0 28.0 37.0 5 32.99584963134115 35.0 33.0 37.0 26.0 37.0 6 33.01758144211514 35.0 33.0 37.0 26.0 37.0 7 32.79563851861196 35.0 33.0 37.0 25.0 37.0 8 32.72014994940159 35.0 33.0 37.0 25.0 37.0 9 33.91076776950497 38.0 33.0 39.0 23.0 39.0 10-11 33.848709809970714 38.0 33.0 39.0 23.0 39.0 12-13 33.71679334048791 38.0 33.0 39.0 22.5 39.0 14-15 34.66443487923628 39.0 33.0 41.0 22.0 41.0 16-17 34.68313287208851 39.0 33.0 41.0 22.5 41.0 18-19 34.72606592913253 39.0 33.5 41.0 22.5 41.0 20-21 34.760170698925975 39.0 34.0 41.0 23.0 41.0 22-23 34.72370389677367 39.0 34.0 41.0 23.0 41.0 24-25 34.682641033227995 39.0 34.0 41.0 23.0 41.0 26-27 34.56657239561932 39.0 33.5 41.0 23.0 41.0 28-29 34.41299994341882 39.0 33.0 41.0 22.0 41.0 30-31 34.22412477726958 38.0 33.0 40.5 21.5 41.0 32-33 33.997196448928065 38.0 33.0 40.0 19.5 41.0 34-35 33.80047180355756 38.0 33.0 40.0 18.0 41.0 36-37 33.57895045622054 38.0 33.0 40.0 16.0 41.0 38-39 33.41190611790961 38.0 32.0 40.0 15.5 41.0 40-41 33.41523234922275 38.0 33.0 40.0 15.0 41.0 42-43 33.25735856792107 38.0 32.0 40.0 11.5 41.0 44-45 33.05598638155936 38.0 32.0 40.0 9.0 41.0 46-47 32.75619888562903 38.0 31.5 40.0 4.5 41.0 48-49 32.42446551655371 38.0 31.0 40.0 2.0 41.0 50-51 32.23345464276406 38.0 31.0 40.0 2.0 41.0 52-53 32.04166485019437 37.5 31.0 40.0 2.0 41.0 54-55 31.84899202019855 37.0 30.0 40.0 2.0 41.0 56-57 31.63661062038017 37.0 30.0 40.0 2.0 41.0 58-59 31.42505034333645 36.5 30.0 40.0 2.0 41.0 60-61 31.18047773066802 36.0 29.5 40.0 2.0 41.0 62-63 30.9992194579894 36.0 29.5 39.5 2.0 41.0 64-65 30.856327631001662 35.5 29.5 39.0 2.0 41.0 66-67 30.557311169588637 35.0 29.0 39.0 2.0 41.0 68-69 30.266857481284152 35.0 29.0 39.0 2.0 40.5 70-71 29.950972407863112 35.0 29.0 38.0 2.0 40.0 72-73 29.65838672215951 35.0 29.0 37.0 2.0 40.0 74-75 29.303462026144217 35.0 29.0 37.0 2.0 39.0 76-77 28.998309521313942 34.5 28.5 36.0 2.0 39.0 78-79 28.713672563786005 34.0 28.0 36.0 2.0 38.5 80-81 28.433001622303003 34.0 28.0 35.5 2.0 37.0 82-83 28.1541660908063 34.0 27.5 35.0 2.0 37.0 84-85 27.932398708093984 34.0 27.0 35.0 2.0 36.5 86-87 27.720554365701787 34.0 27.0 35.0 2.0 36.0 88-89 27.54867465543453 34.0 26.5 35.0 2.0 36.0 90-91 27.41214171823129 34.0 26.0 35.0 2.0 35.5 92-93 27.299256235756154 34.0 26.0 35.0 2.0 35.0 94-95 27.190826521175737 34.0 26.0 35.0 2.0 35.0 96-97 27.078749639410926 34.0 26.0 35.0 2.0 35.0 98-99 26.992199217695624 34.0 26.0 35.0 2.0 35.0 100-101 26.88861762902596 34.0 25.0 35.0 2.0 35.0 102-103 25.635588912685964 31.5 23.5 34.0 2.0 35.0 104-105 26.55600678788643 33.0 25.0 35.0 2.0 35.0 106-107 26.717209119402057 34.0 25.0 35.0 2.0 35.0 108-109 26.71513996421472 34.0 25.0 35.0 2.0 35.0 110-111 26.63942878052717 34.0 25.0 35.0 2.0 35.0 112-113 26.556383145486908 34.0 25.0 35.0 2.0 35.0 114-115 26.4508342013752 34.0 25.0 35.0 2.0 35.0 116-117 26.331584959423875 34.0 25.0 35.0 2.0 35.0 118-119 26.196665745290083 34.0 24.5 35.0 2.0 35.0 120-121 26.002075648109585 34.0 24.0 35.0 2.0 35.0 122-123 25.707906153156905 34.0 21.5 35.0 2.0 35.0 124-125 23.922789415052634 31.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8505345174269436 1101 2 1.0533732862549279 1101 3 0.8516919703914709 1101 4 0.8382053637584761 1101 5 0.9304054016111252 1101 6 0.9954721884606315 1101 7 1.1226206480944114 1101 8 1.1782952176778707 1101 9 1.2479675175105385 1101 10-11 1.3332661467386586 1101 12-13 1.2758459124756527 1101 14-15 1.4293987279934584 1101 16-17 1.4701218430431169 1101 18-19 1.63463978201235 1101 20-21 1.5894337574038744 1101 22-23 1.533774573043452 1101 24-25 1.5208373443621497 1101 26-27 1.5385137181315045 1101 28-29 1.6373416841035393 1101 30-31 1.7006199912856133 1101 32-33 1.7330485122667767 1101 34-35 1.758905333097438 1101 36-37 1.7586340810324046 1101 38-39 1.811923187324922 1101 40-41 1.65038650389738 1101 42-43 1.6126219487502809 1101 44-45 1.618092592980986 1101 46-47 1.5702544540977286 1101 48-49 1.5766796965872771 1101 50-51 1.7698986441208149 1101 52-53 1.8203761994159535 1101 54-55 1.8297924813623005 1101 56-57 1.798601103518095 1101 58-59 1.7308517531421757 1101 60-61 1.6841017135851644 1101 62-63 1.7011882484691405 1101 64-65 1.6288687448354473 1101 66-67 1.7323569669391574 1101 68-69 1.6558761502677726 1101 70-71 1.7110086820570594 1101 72-73 1.5988526529160545 1101 74-75 1.5876300539371364 1101 76-77 1.5482330580038095 1101 78-79 1.577660465389915 1101 80-81 1.425721000990336 1101 82-83 1.4733996509930378 1101 84-85 1.4795959414410902 1101 86-87 1.4269558809691034 1101 88-89 1.4815502113529888 1101 90-91 1.3642750294444745 1101 92-93 1.3083060426306794 1101 94-95 1.3321561849984 1101 96-97 1.4919057713187165 1101 98-99 1.5165576859078804 1101 100-101 1.5073620421730531 1101 102-103 1.400773900694798 1101 104-105 1.3698518474454104 1101 106-107 1.4279973392546665 1101 108-109 1.4642443776672138 1101 110-111 1.307661484491856 1101 112-113 1.4113445274345402 1101 114-115 1.452703553740477 1101 116-117 1.472866635938999 1101 118-119 1.3947555099695599 1101 120-121 1.436849149081933 1101 122-123 1.3153728162390372 1101 124-125 1.7219081509973826 1102 1 0.37323418693143395 1102 2 -0.2798618521522336 1102 3 0.3517061789734548 1102 4 0.35092321246238356 1102 5 0.5552209820025027 1102 6 0.4007207997177247 1102 7 0.40885185091899956 1102 8 0.4802607706252786 1102 9 0.9105195345452657 1102 10-11 0.9934645024359128 1102 12-13 0.8651055383033466 1102 14-15 1.089574846286716 1102 16-17 1.058498006492421 1102 18-19 1.074618741632726 1102 20-21 0.9607374823880193 1102 22-23 1.0663300838322414 1102 24-25 1.0138414667973166 1102 26-27 1.1637210563775042 1102 28-29 0.7120593128663799 1102 30-31 1.0493059407497825 1102 32-33 1.2330447168236418 1102 34-35 1.1964458178241912 1102 36-37 0.7464477403456442 1102 38-39 0.2847225602009402 1102 40-41 0.09136074548998607 1102 42-43 0.3092163170912272 1102 44-45 0.058156414817048585 1102 46-47 0.1854663955350162 1102 48-49 -0.02114522254058926 1102 50-51 -0.10039354660896294 1102 52-53 -0.2559157110349446 1102 54-55 -0.27742893311176786 1102 56-57 -0.2734003017690583 1102 58-59 -0.27345315524166125 1102 60-61 -0.18547041345902215 1102 62-63 -0.12376474642744384 1102 64-65 -0.23577709166076133 1102 66-67 -0.3302538794641201 1102 68-69 -0.21407333141094043 1102 70-71 -0.7915837515810757 1102 72-73 -0.5368697172311307 1102 74-75 -0.4521140261447698 1102 76-77 -0.8089070950595989 1102 78-79 -0.5509622850801357 1102 80-81 -0.4274950531501993 1102 82-83 -0.8208057291935802 1102 84-85 -0.6086986199808564 1102 86-87 -0.35897450865746094 1102 88-89 -0.5819694490424396 1102 90-91 -0.4692786342286581 1102 92-93 -0.4538112988463112 1102 94-95 -0.33970357240465887 1102 96-97 -0.5540308175316362 1102 98-99 -0.7018403354010978 1102 100-101 -0.5682299982961005 1102 102-103 -0.5020183289626026 1102 104-105 -0.3810531224440723 1102 106-107 -0.317014134661882 1102 108-109 -0.3148044356242359 1102 110-111 -0.2707461422078232 1102 112-113 -0.1927190783532211 1102 114-115 -0.20832352236691776 1102 116-117 -0.23262352121180996 1102 118-119 0.002350337656441326 1102 120-121 0.14080516865474024 1102 122-123 0.16665553003926803 1102 124-125 0.33643447969443585 1103 1 0.17449066155375093 1103 2 -0.253246170920324 1103 3 0.10231838238572166 1103 4 0.006899956611782443 1103 5 0.29866756902757174 1103 6 0.2560713945083961 1103 7 0.40684879541728236 1103 8 0.48919110077389405 1103 9 0.5238701387908193 1103 10-11 0.5779846200546004 1103 12-13 0.49805955371164856 1103 14-15 0.6594461062911563 1103 16-17 0.4948287531284521 1103 18-19 0.5670483382403191 1103 20-21 0.5380311178362476 1103 22-23 0.6088733950569178 1103 24-25 0.6009291749819283 1103 26-27 0.6573180350709293 1103 28-29 0.6100134937350603 1103 30-31 0.6145736595868883 1103 32-33 0.6755746139387995 1103 34-35 0.6716111009269028 1103 36-37 0.7205909116381122 1103 38-39 0.7234826197981903 1103 40-41 0.5925697881362169 1103 42-43 0.5889837231294308 1103 44-45 0.6659099276985359 1103 46-47 0.783636009760194 1103 48-49 0.700139128216513 1103 50-51 0.660009971866657 1103 52-53 0.7183260470682811 1103 54-55 0.6616175305271348 1103 56-57 0.7828765166011422 1103 58-59 0.8863392234955754 1103 60-61 0.8248210253056669 1103 62-63 0.7010603777072397 1103 64-65 0.6325560368168652 1103 66-67 0.6488960441877296 1103 68-69 0.6833222236928798 1103 70-71 0.6509891849309852 1103 72-73 0.6950630924594883 1103 74-75 0.7946757469854084 1103 76-77 0.7624050491020924 1103 78-79 0.6672995575497076 1103 80-81 0.6826348844633578 1103 82-83 0.7115836329645262 1103 84-85 0.8392993487959792 1103 86-87 0.8052260104153888 1103 88-89 0.8266493428446466 1103 90-91 0.8138938085811418 1103 92-93 0.679067668460295 1103 94-95 0.6678687022658778 1103 96-97 0.7552472354881736 1103 98-99 0.7757089560345172 1103 100-101 0.6870547865975247 1103 102-103 0.5050169873740096 1103 104-105 0.5374586244551196 1103 106-107 0.6130267537500629 1103 108-109 0.6314070534719249 1103 110-111 0.5695436183158513 1103 112-113 0.5006088696386435 1103 114-115 0.631261357233079 1103 116-117 0.7659129423167812 1103 118-119 0.6348554432910767 1103 120-121 0.6470323883057603 1103 122-123 0.712266358554551 1103 124-125 0.9164607721369968 1104 1 0.44742133030610987 1104 2 0.6340209773836634 1104 3 0.4346913999025297 1104 4 0.15601817994657097 1104 5 0.4488028655326417 1104 6 0.6765544349458708 1104 7 0.7747691296790151 1104 8 0.5124001131717435 1104 9 0.734310030613166 1104 10-11 0.7464129977638194 1104 12-13 0.7101446179116593 1104 14-15 0.8413621247609342 1104 16-17 0.7528765737111982 1104 18-19 0.6446371295375783 1104 20-21 0.7168580940668292 1104 22-23 0.7675366235305745 1104 24-25 0.9047640367628844 1104 26-27 0.8254584855159024 1104 28-29 0.9880831128634426 1104 30-31 0.8939767601759954 1104 32-33 0.9249966193789447 1104 34-35 1.1474802002323585 1104 36-37 1.1607257205882036 1104 38-39 1.1634943304220329 1104 40-41 1.189634074492517 1104 42-43 1.1878217233129078 1104 44-45 1.0398251876667501 1104 46-47 1.0570386019522786 1104 48-49 0.9260707536559423 1104 50-51 0.9669586723372632 1104 52-53 1.0179509088734306 1104 54-55 0.6902366352757134 1104 56-57 0.8509603120416855 1104 58-59 1.0129996784885762 1104 60-61 1.1148326335801784 1104 62-63 1.0157790203393056 1104 64-65 1.0061527577677545 1104 66-67 1.1063958585632747 1104 68-69 0.8291188857277767 1104 70-71 0.4789686715207111 1104 72-73 0.5499652902344891 1104 74-75 0.9774276337952408 1104 76-77 1.0372188058936693 1104 78-79 1.0593440467549264 1104 80-81 0.9495421092650922 1104 82-83 1.0195296810988559 1104 84-85 0.930346518250925 1104 86-87 0.9733437113964385 1104 88-89 0.9609739945359301 1104 90-91 0.9845886190795063 1104 92-93 0.9735648290108436 1104 94-95 0.9651023581484601 1104 96-97 1.0163079876291796 1104 98-99 1.0477255057345083 1104 100-101 1.1037513206585423 1104 102-103 0.9897453978536142 1104 104-105 1.0438070275171896 1104 106-107 1.030613773473526 1104 108-109 1.0983206797588068 1104 110-111 1.1143524325654077 1104 112-113 0.959516871178721 1104 114-115 0.9976348675153695 1104 116-117 1.0521018497712191 1104 118-119 1.0460462814259905 1104 120-121 1.05302657895799 1104 122-123 1.0685558549270127 1104 124-125 1.4788259385300186 1105 1 0.06463102848780977 1105 2 -0.26751952602803186 1105 3 0.0655808592803595 1105 4 0.10429783690059224 1105 5 -0.1912507546448765 1105 6 0.2542788597082861 1105 7 0.29524130539221716 1105 8 0.39017715381004336 1105 9 0.5375385671477559 1105 10-11 0.4510046813398887 1105 12-13 0.4788786462168346 1105 14-15 0.612483339570062 1105 16-17 0.5151438982809609 1105 18-19 0.6639248524265682 1105 20-21 0.6258872880891104 1105 22-23 0.5975622368876401 1105 24-25 0.5987347265495089 1105 26-27 0.6280081562955644 1105 28-29 0.5385839075079559 1105 30-31 0.6405267183178722 1105 32-33 0.6781269524405573 1105 34-35 0.7790496249654453 1105 36-37 0.8503604113659264 1105 38-39 0.860765287338225 1105 40-41 0.5555271845434504 1105 42-43 0.5944104918485067 1105 44-45 0.7085801633582562 1105 46-47 0.6712237577876365 1105 48-49 0.719103750096167 1105 50-51 0.38982399988621097 1105 52-53 0.5851944930720308 1105 54-55 0.5048092444513692 1105 56-57 0.48688264213877375 1105 58-59 0.12586563464238054 1105 60-61 0.3210351858258562 1105 62-63 0.49100003084268806 1105 64-65 0.24154099365695103 1105 66-67 0.36876205552954744 1105 68-69 0.410178260846898 1105 70-71 0.46433871818775074 1105 72-73 0.46948224313933906 1105 74-75 0.36587895302241336 1105 76-77 0.4057509109245565 1105 78-79 0.33229192679879205 1105 80-81 0.3490906866754919 1105 82-83 0.3277405983707915 1105 84-85 0.31995356324363655 1105 86-87 0.37775861589248194 1105 88-89 0.40525252659880806 1105 90-91 0.203555057078173 1105 92-93 0.2799662164865424 1105 94-95 0.3384229019081566 1105 96-97 0.39119807341267077 1105 98-99 0.21518029566686536 1105 100-101 0.3330317806873353 1105 102-103 -0.05654113270207972 1105 104-105 0.17064326843815536 1105 106-107 0.22259560697644076 1105 108-109 0.1448734968615497 1105 110-111 0.12862735244119605 1105 112-113 0.10270100768457269 1105 114-115 0.16279437920485407 1105 116-117 -0.05760939884694949 1105 118-119 0.04779253838646014 1105 120-121 0.1123625706362148 1105 122-123 0.045799900957749884 1105 124-125 0.47923874682129863 1106 1 -0.1781238333127959 1106 2 -0.6260624573177545 1106 3 -0.06462428462656078 1106 4 -0.1760120564400367 1106 5 0.11510715324993726 1106 6 0.2881708195296895 1106 7 0.2785746634977926 1106 8 0.3239308473516189 1106 9 0.3884201865390722 1106 10-11 0.29877140780675404 1106 12-13 0.30574843661393203 1106 14-15 0.30610387982297027 1106 16-17 0.20367244185137423 1106 18-19 0.2890697700538922 1106 20-21 0.1866929594961917 1106 22-23 0.19934717615601016 1106 24-25 0.30432992182414864 1106 26-27 0.5389034165230342 1106 28-29 0.4938552676230543 1106 30-31 0.4495144166692313 1106 32-33 0.4375357293952362 1106 34-35 0.27698371271269906 1106 36-37 0.4614323410444712 1106 38-39 0.40388478418422125 1106 40-41 0.2975492833668625 1106 42-43 0.12852893191058 1106 44-45 0.17673553660661412 1106 46-47 0.11982574858425465 1106 48-49 0.22644131973094517 1106 50-51 0.2759887883213352 1106 52-53 0.09967306458553082 1106 54-55 0.10741176066727931 1106 56-57 -0.43241384594974264 1106 58-59 0.09950261004056671 1106 60-61 0.13149853578632076 1106 62-63 0.09599498845242138 1106 64-65 0.10980213819407325 1106 66-67 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2216 74-75 -1.5366021706939712 2216 76-77 -1.5962648579216214 2216 78-79 -1.6620761496068006 2216 80-81 -1.6966881457107057 2216 82-83 -1.6112251848068375 2216 84-85 -1.6415801192578634 2216 86-87 -1.6761073003254836 2216 88-89 -1.675740889769667 2216 90-91 -1.6269944688715618 2216 92-93 -1.6262516768168105 2216 94-95 -1.5564376053334819 2216 96-97 -1.5637551677849437 2216 98-99 -1.698756620113958 2216 100-101 -1.6386400105080519 2216 102-103 -1.4991277741567437 2216 104-105 -1.5144170316191712 2216 106-107 -1.603749577722546 2216 108-109 -1.6726482767958117 2216 110-111 -1.538239534585113 2216 112-113 -1.4635189109991877 2216 114-115 -1.6895237324731163 2216 116-117 -1.645478924430222 2216 118-119 -1.6438028036966905 2216 120-121 -1.7583588541583666 2216 122-123 -1.8425532946279048 2216 124-125 -2.1449994451127807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75445.0 3 24964.0 4 11489.0 5 9096.0 6 10310.0 7 12066.0 8 13413.0 9 14401.0 10 15833.0 11 17767.0 12 20014.0 13 23374.0 14 27723.0 15 24501.0 16 15924.0 17 13409.0 18 12344.0 19 11367.0 20 11079.0 21 11446.0 22 12996.0 23 16078.0 24 21039.0 25 28125.0 26 34517.0 27 37696.0 28 42912.0 29 51123.0 30 58555.0 31 68651.0 32 84168.0 33 103027.0 34 134046.0 35 183702.0 36 293827.0 37 450186.0 38 158985.0 39 596.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.489699027490154 19.47331551628811 13.89734169499614 23.1396437612256 2 15.550887861563348 20.881442839969726 38.99393003962541 24.573739258841513 3 19.329197651046243 25.861726727743424 29.428381676231357 25.380693944978976 4 12.695711194459802 17.236187693331903 36.07241958344534 33.99568152876296 5 12.972348499253778 39.188171379755254 33.27645842628261 14.563021694708361 6 31.50917774560174 36.98285033721456 17.077637726475448 14.430334190708258 7 27.942745859384893 33.61533406210447 20.21301018810922 18.228909890401415 8 26.67671275064191 35.78873354408775 18.831259183400864 18.70329452186948 9 27.001337595333812 15.988345667285683 19.319533158821358 37.69078357855914 10-11 25.183924726170947 27.457017755002944 26.26736809201083 21.09168942681528 12-13 26.22799710972204 24.73358148663803 27.299885817324416 21.738535586315518 14-15 23.02351876664102 25.40651509396422 26.06494409359459 25.505022045800168 16-17 22.040002532246135 29.19401527278343 26.351940008955616 22.41404218601481 18-19 21.83655840669897 26.765453742256174 29.83949419162142 21.55849365942344 20-21 22.82863123057023 25.867362532424497 29.925265390418073 21.378740846587203 22-23 22.382818985675687 25.900313237120592 29.84926217214221 21.86760560506151 24-25 22.173143974985553 26.03701243951147 29.98899913458622 21.800844450916752 26-27 21.944407599687228 26.342202974314926 29.840311215039094 21.873078210958756 28-29 21.738837043420027 26.281679663332707 30.227405326236877 21.75207796701039 30-31 21.915861930791014 26.01150453066839 30.140817570218637 21.93181596832196 32-33 21.715856736453212 26.17790885235744 29.970540684187046 22.1356937270023 34-35 22.24208489588599 26.092039955588415 29.732575083689127 21.933300064836466 36-37 22.33827290123245 25.858920857770684 29.63613199925424 22.166674241742626 38-39 22.053117669374835 26.139832501157134 29.81447866008346 21.992571169384572 40-41 22.144459172041106 25.95425086981969 29.893437232456822 22.007852725682383 42-43 22.22682652859622 25.930598081619742 29.493055819652593 22.349519570131445 44-45 22.232507835565816 26.227023171384396 29.11811274866733 22.422356244382463 46-47 22.34715429131145 26.475029612363265 28.57147826216008 22.6063378341652 48-49 22.65336514246863 26.624575525207845 28.11685775955225 22.60520157277128 50-51 22.502873698487637 26.78092202763806 27.964187815240145 22.75201645863416 52-53 22.841149729569786 26.831444665925236 27.76607763494379 22.56132796956118 54-55 22.44106977387007 26.731500041276433 27.909107436529368 22.91832274832413 56-57 22.48476537741999 27.142500335686968 27.758749718521546 22.613984568371492 58-59 22.671884239451806 26.65704314352963 27.90265168643547 22.76842093058309 60-61 22.606036377060693 26.69254250777064 28.152568831932562 22.54885228323611 62-63 22.584540166608384 26.767628515801455 28.05269377430788 22.595137543282284 64-65 22.373118670542645 26.51735377434621 28.12744064988767 22.98208690522348 66-67 22.3429802698644 27.0058491953878 27.851018971391255 22.800151563356543 68-69 22.35165295887105 26.756938383095395 28.054061925782186 22.837346732251365 70-71 22.21022319884018 26.851735047959508 27.98567754107469 22.952364212125627 72-73 22.50439895482503 26.784626058694162 27.698249786429237 23.012725200051573 74-75 22.32590658436023 26.921300268531702 27.624769846533436 23.12802330057463 76-77 22.38518426449568 26.898089875029797 27.739433464706792 22.97729239576773 78-79 22.342493300695576 27.00640573158074 27.62249129716528 23.0286096705584 80-81 22.202882580548216 27.222029753366233 27.606248605760246 22.968839060325305 82-83 22.478682344909593 27.09781680127113 27.418497593444748 23.00500326037453 84-85 22.359885056724952 26.91450769272153 27.363168620263295 23.362438630290224 86-87 22.39566569612938 26.732566735646234 27.664347456675976 23.207420111548405 88-89 22.346787547981613 26.729732355764135 27.578021064719323 23.345459031534933 90-91 22.23068621408195 26.997420917329755 27.639617603804485 23.13227526478381 92-93 22.281368433330883 26.81116687680255 27.66674497001477 23.2407197198518 94-95 22.120620871776843 26.754248458162856 27.60714017384335 23.517990496216946 96-97 22.31429855552015 26.678906920729627 27.510330468306833 23.49646405544339 98-99 22.065753938014907 26.575147987174986 27.47969828843666 23.879399786373444 100-101 21.906794419835105 26.77271898770811 27.72193974687137 23.598546845585417 102-103 22.293100713572155 26.514706005118278 27.568159451329517 23.62403382998005 104-105 22.021395106377256 26.400476951517348 27.790263770328643 23.78786417177675 106-107 21.96503415097481 26.565966085092903 27.757110902200715 23.711888861731566 108-109 22.068669657277816 26.60945161436189 27.273353376379895 24.048525351980402 110-111 21.724386214093162 26.691138873590535 27.64114901881214 23.94332589350416 112-113 21.80879828067419 26.594986350949867 27.625413112178215 23.970802256197725 114-115 21.55425253942827 26.62884230268705 27.498453293163788 24.318451864720892 116-117 21.42740866545404 26.528457086885503 27.651523935230315 24.392610312430143 118-119 21.500732308873875 26.710328476936677 27.29721908139991 24.491720132789535 120-121 21.275567029601785 26.6670237812826 27.556331932006305 24.501077257109305 122-123 21.419966249144576 26.560745162489475 27.41541588989531 24.603872698470635 124-125 21.26049491825011 26.51897811709748 27.297463395433297 24.923063569219114 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 661.0 1 488.5 2 528.5 3 796.5 4 632.5 5 491.5 6 907.5 7 2151.5 8 3580.0 9 4124.5 10 3231.5 11 2021.0 12 1691.0 13 1785.5 14 2026.5 15 2325.0 16 2645.5 17 3016.0 18 3470.0 19 3865.5 20 4309.5 21 4929.5 22 5774.0 23 6815.0 24 8033.0 25 9637.5 26 12093.0 27 14782.0 28 17188.0 29 20195.5 30 23808.0 31 27163.0 32 31066.5 33 35451.0 34 40099.0 35 43467.5 36 47241.0 37 52853.5 38 56326.5 39 57665.0 40 58417.0 41 61783.5 42 66320.5 43 71190.0 44 74970.0 45 76527.0 46 77866.0 47 79399.0 48 85043.5 49 88226.0 50 86126.5 51 83041.5 52 79963.5 53 78241.5 54 75715.0 55 69765.5 56 62404.5 57 56006.5 58 50756.5 59 45357.5 60 38304.0 61 30980.5 62 24403.5 63 18593.5 64 14190.5 65 11208.5 66 8503.0 67 6632.0 68 4864.0 69 3110.5 70 2192.0 71 1687.5 72 1411.0 73 1181.5 74 912.5 75 721.0 76 505.5 77 266.5 78 142.5 79 82.5 80 54.5 81 36.0 82 22.5 83 15.5 84 13.0 85 11.0 86 7.5 87 3.5 88 3.5 89 2.5 90 1.5 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09632713939469269 2 9.275603215666122E-5 3 0.0 4 0.10161423322762238 5 0.0 6 0.0 7 0.0024580348521515225 8 0.0060291420901829795 9 0.003988509382736433 10-11 6.02914209018298E-4 12-13 0.0 14-15 4.637801607833061E-5 16-17 2.550790884308184E-4 18-19 0.0019246876672507204 20-21 0.001437718498428249 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.3189008039165306E-5 52-53 0.0 54-55 0.0 56-57 0.007768317693120377 58-59 8.811823054882816E-4 60-61 0.0 62-63 0.0 64-65 2.7826809646998367E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0011130723858799347 76-77 0.0 78-79 0.0 80-81 4.637801607833061E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0013681514743107532 90-91 4.637801607833061E-5 92-93 0.0022957117958773655 94-95 0.0 96-97 0.008950957103117808 98-99 0.0044059115274414085 100-101 0.002643546916464845 102-103 0.0 104-105 0.0 106-107 0.0015304745305849102 108-109 6.956702411749592E-5 110-111 3.01457104509149E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010898833778407696 122-123 0.004660990615872227 124-125 0.08431523323040506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2156194.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.86205729980006 #Duplication Level Percentage of deduplicated Percentage of total 1 76.17813921407452 40.26933160126553 2 12.46463439907189 13.17812435649594 3 4.6629074904032795 7.39472648824095 4 2.202995166816351 4.658194269577141 5 1.196982327475901 3.1637474190939554 6 0.747601015984064 2.3711836646602995 7 0.4821252058174713 1.7840291178920744 8 0.3359972653018064 1.4209205352768168 9 0.26036229802514427 1.2386958045221517 >10 1.3049229510583111 12.612294850191248 >50 0.1001093291525031 3.6439171384197917 >100 0.05899300143299591 5.552645532541308 >500 0.0033490155138004535 1.200303062921613 >1k 6.169239104369198E-4 0.45316518758882335 >5k 2.643959616158228E-4 1.0587209713124304 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9342 0.4332634262037646 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8289 0.38442737527328247 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5094 0.23624961390301616 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2476 0.1148319678099466 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.637801607833061E-5 0.0 12-13 0.0 0.0 0.0 1.3913404823499183E-4 0.0 14-15 0.0 0.0 0.0 1.6232305627415713E-4 0.0 16-17 0.0 0.0 0.0 2.3189008039165306E-4 0.0 18-19 0.0 0.0 0.0 2.7826809646998367E-4 0.0 20-21 0.0 0.0 0.0 4.4059115274414085E-4 0.0 22-23 0.0 0.0 0.0 8.116152813707857E-4 0.0 24-25 0.0 0.0 0.0 0.0011362613939191 2.3189008039165306E-5 26-27 0.0 0.0 0.0 0.0013681514743107532 4.637801607833061E-5 28-29 0.0 0.0 0.0 0.0021565777476423736 4.637801607833061E-5 30-31 0.0 0.0 0.0 0.0045682345837155655 4.637801607833061E-5 32-33 0.0 0.0 0.0 0.008811823054882817 4.637801607833061E-5 34-35 0.0 0.0 0.0 0.01433080696820416 4.637801607833061E-5 36-37 0.0 0.0 0.0 0.025878932971708485 4.637801607833061E-5 38-39 0.0 0.0 0.0 0.045682345837155656 4.637801607833061E-5 40-41 0.0 0.0 0.0 0.06998442626220089 4.637801607833061E-5 42-43 0.0 0.0 0.0 0.09240819703607375 4.637801607833061E-5 44-45 0.0 0.0 0.0 0.11879728818464386 4.637801607833061E-5 46-47 0.0 0.0 0.0 0.15160973456006277 4.637801607833061E-5 48-49 0.0 0.0 0.0 0.1913788833472313 4.637801607833061E-5 50-51 4.637801607833061E-5 0.0 0.0 0.23543799862164536 4.637801607833061E-5 52-53 4.637801607833061E-5 0.0 0.0 0.2779202613493962 4.637801607833061E-5 54-55 4.637801607833061E-5 0.0 0.0 0.32392725329910016 4.637801607833061E-5 56-57 4.637801607833061E-5 0.0 0.0 0.38062437795485937 4.637801607833061E-5 58-59 4.637801607833061E-5 0.0 0.0 0.4460405696333447 4.637801607833061E-5 60-61 6.956702411749592E-5 0.0 0.0 0.5223092170741594 4.637801607833061E-5 62-63 9.275603215666122E-5 0.0 0.0 0.5996445588847756 9.275603215666122E-5 64-65 9.275603215666122E-5 0.0 0.0 0.6730841473448121 9.275603215666122E-5 66-67 9.275603215666122E-5 0.0 0.0 0.7581414288324706 9.275603215666122E-5 68-69 9.275603215666122E-5 0.0 0.0 0.8668978765361559 9.275603215666122E-5 70-71 9.275603215666122E-5 0.0 0.0 0.988964814854322 9.275603215666122E-5 72-73 9.275603215666122E-5 0.0 0.0 1.1156695547803213 9.275603215666122E-5 74-75 1.3913404823499183E-4 0.0 0.0 1.2392901566371115 9.275603215666122E-5 76-77 1.8551206431332244E-4 0.0 0.0 1.3754096338270119 9.275603215666122E-5 78-79 1.8551206431332244E-4 0.0 0.0 1.5330485104772575 9.275603215666122E-5 80-81 1.8551206431332244E-4 0.0 0.0 1.7196040801523425 9.275603215666122E-5 82-83 1.8551206431332244E-4 0.0 0.0 1.9223223884307257 9.275603215666122E-5 84-85 1.8551206431332244E-4 0.0 0.0 2.119011554618926 9.275603215666122E-5 86-87 1.8551206431332244E-4 0.0 0.0 2.325903884344359 9.275603215666122E-5 88-89 1.8551206431332244E-4 0.0 0.0 2.5643564540110955 9.275603215666122E-5 90-91 1.8551206431332244E-4 0.0 0.0 2.8403288386852017 9.275603215666122E-5 92-93 1.8551206431332244E-4 0.0 0.0 3.139165585285925 9.275603215666122E-5 94-95 1.8551206431332244E-4 0.0 0.0 3.4316949216999957 9.275603215666122E-5 96-97 1.8551206431332244E-4 0.0 0.0 3.725685165620533 9.275603215666122E-5 98-99 1.8551206431332244E-4 0.0 0.0 4.052789313020999 9.275603215666122E-5 100-101 1.8551206431332244E-4 0.0 0.0 4.438747162824866 9.275603215666122E-5 102-103 1.8551206431332244E-4 0.0 0.0 4.851650639970244 9.275603215666122E-5 104-105 1.8551206431332244E-4 0.0 0.0 5.260333717652493 9.275603215666122E-5 106-107 1.8551206431332244E-4 0.0 0.0 5.668344314101606 9.275603215666122E-5 108-109 1.8551206431332244E-4 0.0 0.0 6.091984301969118 9.275603215666122E-5 110-111 1.8551206431332244E-4 0.0 0.0 6.58975954853784 9.275603215666122E-5 112-113 1.8551206431332244E-4 0.0 0.0 7.133495409040188 9.275603215666122E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6740 0.0 58.55628 1 GTATCAA 11215 0.0 47.524067 1 ATCAACG 14470 0.0 36.5919 3 TCAACGC 14570 0.0 36.218117 4 TATCAAC 14620 0.0 36.053516 2 CAACGCA 14795 0.0 35.630924 5 AACGCAG 15170 0.0 34.63247 6 GTACATG 13270 0.0 32.526928 1 TACATGG 13530 0.0 31.078398 2 ACGCAGA 17405 0.0 29.501568 7 ACATGGG 13785 0.0 29.38014 3 CGCAGAG 17510 0.0 29.22272 8 GAGTACT 10495 0.0 26.786602 12-13 GCAGAGT 18765 0.0 26.728664 9 GTACTTT 11080 0.0 25.694517 14-15 GTGGTAT 3615 0.0 25.232395 1 TGGTATC 3525 0.0 25.00658 2 CATGGGA 9395 0.0 24.850836 4 AGTACTT 10520 0.0 24.715189 12-13 CATGGGG 4970 0.0 24.566833 4 >>END_MODULE