FastQCFastQC Report
Thu 26 May 2016
SRR1364565_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364565_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179291
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30560.1402290928563464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.10200565229700853No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69000.043.9771581
GGTATCA44000.040.0262681
TCAACGC81850.036.9267544
ATCAACG81950.036.8090933
CAACGCA83350.036.4049725
GTACATG112300.036.1335561
AACGCAG84550.035.814636
TACATGG114700.034.339242
TATCAAC88200.034.0658232
ACATGGG118300.032.5901533
ACGCAGA98450.030.3954497
CGCAGAG99400.029.149348
CATGGGA75900.028.14164
CATGGGG53000.026.380814
TAGCGTG1401.0566904E-425.4987745
GCAGAGT107500.025.0164749
GAGTACT40300.023.5527412-13
ATGGGAG29300.023.3521215
ATGGGGA33650.022.9855235
GAGTACA88550.022.9796621
Produced by FastQC (version 0.11.5)