FastQCFastQC Report
Thu 26 May 2016
SRR1364565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179291
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77090.3537388994861173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67660.31046794576768316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.21424399036200306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53300.055.3662721
GTATCAA89550.048.132671
ATCAACG116600.037.0396963
TCAACGC116750.036.890064
CAACGCA119000.036.299375
TATCAAC120100.036.009872
AACGCAG122300.035.368566
GTACATG106400.031.26511
ACGCAGA142050.030.07417
TACATGG107350.030.0208632
CGCAGAG143500.029.7702148
ACATGGG112350.028.207623
GCAGAGT153900.027.1386369
GAGTACT88050.026.65735812-13
GTACTTT93350.025.23947514-15
CATGGGG50300.024.9886254
AGTACTT91000.024.84515212-13
AGAGTAC146150.023.48856710-11
CAGAGTA152000.023.21082910-11
ATTCGCG4450.022.72837680-81
Produced by FastQC (version 0.11.5)