##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364565_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2179291 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.05802575241214 31.0 30.0 34.0 26.0 34.0 2 29.923175473124058 31.0 30.0 34.0 26.0 34.0 3 30.23254030783406 31.0 30.0 34.0 26.0 34.0 4 33.811647916684834 35.0 35.0 37.0 29.0 37.0 5 33.22068461715301 35.0 33.0 37.0 27.0 37.0 6 33.24015012221865 35.0 33.0 37.0 27.0 37.0 7 33.03044797597017 35.0 33.0 37.0 26.0 37.0 8 32.96344453310733 35.0 33.0 37.0 26.0 37.0 9 34.20333769101969 38.0 34.0 39.0 25.0 39.0 10-11 34.1365976365708 38.0 34.0 39.0 25.0 39.0 12-13 34.00525652609036 38.0 34.0 39.0 25.0 39.0 14-15 34.97781227931469 39.0 34.0 41.0 25.0 41.0 16-17 34.97481818628168 39.0 34.0 41.0 25.0 41.0 18-19 34.98520963928176 39.0 34.0 41.0 25.0 41.0 20-21 34.99605559789858 39.0 34.0 41.0 25.0 41.0 22-23 34.94769514488887 39.0 34.0 41.0 24.5 41.0 24-25 34.89979195068488 39.0 34.0 41.0 24.0 41.0 26-27 34.774030636569414 39.0 34.0 41.0 24.0 41.0 28-29 34.61354380851387 39.0 33.5 41.0 23.5 41.0 30-31 34.441834293813905 38.0 33.0 40.5 23.0 41.0 32-33 34.237944129535705 38.0 33.0 40.0 22.5 41.0 34-35 34.04076761662394 38.0 33.0 40.0 21.0 41.0 36-37 33.83435576065794 38.0 33.0 40.0 18.5 41.0 38-39 33.66140662261259 38.0 33.0 40.0 17.5 41.0 40-41 33.68094302229487 38.0 33.0 40.0 17.0 41.0 42-43 33.537352515106974 38.0 33.0 40.0 15.5 41.0 44-45 33.36254841597565 38.0 32.0 40.0 14.5 41.0 46-47 33.10553386399522 38.0 32.0 40.0 9.5 41.0 48-49 32.8176677644243 38.0 32.0 40.0 7.5 41.0 50-51 32.63119152054499 38.0 31.0 40.0 6.0 41.0 52-53 32.43310829072391 37.5 31.0 40.0 2.0 41.0 54-55 32.228119833468774 37.0 31.0 40.0 2.0 41.0 56-57 32.00154293299977 37.0 31.0 40.0 2.0 41.0 58-59 31.778247833813843 36.5 30.5 40.0 2.0 41.0 60-61 31.52833696830758 36.0 30.0 40.0 2.0 41.0 62-63 31.340672264511717 36.0 30.0 39.0 2.0 41.0 64-65 31.196087626663903 35.0 30.0 39.0 2.0 41.0 66-67 30.885476515068433 35.0 30.0 39.0 2.0 41.0 68-69 30.598380161254276 35.0 30.0 38.5 2.0 40.5 70-71 30.282250970613838 35.0 29.0 37.5 2.0 40.0 72-73 29.996480736166028 35.0 29.0 37.0 2.0 39.5 74-75 29.656563304304015 35.0 29.0 36.5 2.0 39.0 76-77 29.354013530088455 34.5 29.0 36.0 2.0 39.0 78-79 29.075743211897816 34.0 29.0 36.0 2.0 38.0 80-81 28.80890734647186 34.0 29.0 35.0 2.0 37.0 82-83 28.547390412753508 34.0 29.0 35.0 2.0 37.0 84-85 28.335147073061833 34.0 28.0 35.0 2.0 36.0 86-87 28.132683748980746 34.0 28.0 35.0 2.0 36.0 88-89 27.97049063204501 34.0 27.5 35.0 2.0 36.0 90-91 27.841564527178793 34.0 27.0 35.0 2.0 35.0 92-93 27.734235583958267 34.0 27.0 35.0 2.0 35.0 94-95 27.632727341139848 34.0 27.0 35.0 2.0 35.0 96-97 27.522074839936472 34.0 27.0 35.0 2.0 35.0 98-99 27.434391735660817 34.0 27.0 35.0 2.0 35.0 100-101 27.333615152818048 34.0 27.0 35.0 2.0 35.0 102-103 26.051526849787386 31.5 24.5 34.0 2.0 35.0 104-105 26.994313288129028 33.5 25.5 35.0 2.0 35.0 106-107 27.156134724550324 34.0 26.0 35.0 2.0 35.0 108-109 27.161374043209467 34.0 26.0 35.0 2.0 35.0 110-111 27.085025588597393 34.0 26.0 35.0 2.0 35.0 112-113 27.00196944786171 34.0 25.5 35.0 2.0 35.0 114-115 26.893811794753432 34.0 25.0 35.0 2.0 35.0 116-117 26.761865670991163 34.0 25.0 35.0 2.0 35.0 118-119 26.618488994815287 34.0 25.0 35.0 2.0 35.0 120-121 26.420012517832635 34.0 25.0 35.0 2.0 35.0 122-123 26.121568207274752 34.0 24.5 35.0 2.0 35.0 124-125 24.302900576380118 31.5 12.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7510678259345127 1101 2 0.8952823592623673 1101 3 0.6741142501855784 1101 4 0.6094455950815671 1101 5 0.7345591893743944 1101 6 0.8335950355290578 1101 7 0.9029275465936024 1101 8 0.9043897799116465 1101 9 1.154589973176428 1101 10-11 1.0743098639877005 1101 12-13 0.973760156234988 1101 14-15 1.2591382694164466 1101 16-17 1.2650557546046812 1101 18-19 1.3416830818757575 1101 20-21 1.2777383715016981 1101 22-23 1.1967122608880203 1101 24-25 1.2238220541036071 1101 26-27 1.1649842918402626 1101 28-29 1.3608286270759677 1101 30-31 1.3191843546781499 1101 32-33 1.4260933057967335 1101 34-35 1.4572325635150492 1101 36-37 1.349460354024174 1101 38-39 1.3561702723759907 1101 40-41 1.1704050623966822 1101 42-43 1.1859000454345079 1101 44-45 1.1948946404984326 1101 46-47 1.2759689701095027 1101 48-49 1.4319578123796788 1101 50-51 1.402056239804132 1101 52-53 1.3852611585560552 1101 54-55 1.4022812123821637 1101 56-57 1.3898320129354502 1101 58-59 1.4451697856209798 1101 60-61 1.3845707547998387 1101 62-63 1.4112007043228658 1101 64-65 1.3476260805727662 1101 66-67 1.382556068502101 1101 68-69 1.4746798592885 1101 70-71 1.3728081038891702 1101 72-73 1.3744544447764646 1101 74-75 1.415876827900501 1101 76-77 1.3967164253778002 1101 78-79 1.3696809492018076 1101 80-81 1.1145996651397816 1101 82-83 1.3542990084057394 1101 84-85 1.3753022865369289 1101 86-87 1.4105630530155295 1101 88-89 1.3493043042216435 1101 90-91 1.2995018668306813 1101 92-93 1.2090153280814881 1101 94-95 1.223203990880183 1101 96-97 1.2713165697464284 1101 98-99 1.2963768286427069 1101 100-101 1.315879590226892 1101 102-103 1.2116503849482498 1101 104-105 1.3364338035439687 1101 106-107 1.2722510433097511 1101 108-109 1.2899109764535517 1101 110-111 1.2432883474157883 1101 112-113 1.268948377068405 1101 114-115 1.2984726659801709 1101 116-117 1.32475554311673 1101 118-119 1.4434462609813572 1101 120-121 1.4717541273966752 1101 122-123 1.3288048855848942 1101 124-125 1.5302946703690488 1102 1 0.36492176476450666 1102 2 -0.4327950058677459 1102 3 0.11857229092866106 1102 4 0.14248377919085442 1102 5 0.49636150956664693 1102 6 0.37169171932416134 1102 7 0.3430768494176135 1102 8 0.37553961018827664 1102 9 0.7145761822767369 1102 10-11 0.7616973381043906 1102 12-13 0.7873725900049848 1102 14-15 0.8975591572747206 1102 16-17 1.0093637463382592 1102 18-19 1.043088694292507 1102 20-21 0.864467549567749 1102 22-23 0.8899156806213213 1102 24-25 0.857659507260415 1102 26-27 1.0211555889648452 1102 28-29 0.6547975345318591 1102 30-31 0.8339270274894446 1102 32-33 1.0486309299286063 1102 34-35 1.0507588921190916 1102 36-37 0.6243183588884875 1102 38-39 0.0884918864409272 1102 40-41 -0.2023170058361785 1102 42-43 0.23214479446826175 1102 44-45 -0.08462306077083781 1102 46-47 0.052937172072141436 1102 48-49 -0.1591270784950396 1102 50-51 -0.24941081209528448 1102 52-53 -0.5161866829332666 1102 54-55 -0.5406312481712554 1102 56-57 -0.4768847602801287 1102 58-59 -0.4296667809089456 1102 60-61 -0.46152940629697525 1102 62-63 -0.4659129361508505 1102 64-65 -0.44311982751322887 1102 66-67 -0.5379572471876166 1102 68-69 -0.40203120831445105 1102 70-71 -0.8315945258154116 1102 72-73 -0.6815235303796214 1102 74-75 -0.6533690888362216 1102 76-77 -0.9337209446740466 1102 78-79 -0.628183916412798 1102 80-81 -0.7265290351355809 1102 82-83 -0.8924065391644049 1102 84-85 -0.8980063685478186 1102 86-87 -0.5773181020602749 1102 88-89 -0.6576318861901989 1102 90-91 -0.6816571428806526 1102 92-93 -0.5792964398146161 1102 94-95 -0.4565658186289028 1102 96-97 -0.6358887887159561 1102 98-99 -0.7535607653131109 1102 100-101 -0.5633287340328437 1102 102-103 -0.6352242335619991 1102 104-105 -0.5230626585025888 1102 106-107 -0.3370696253613694 1102 108-109 -0.3700855458581138 1102 110-111 -0.3597994976575478 1102 112-113 -0.09692444296083735 1102 114-115 -0.19217527143072033 1102 116-117 -0.3441664498596566 1102 118-119 -0.2258485830992214 1102 120-121 0.08095885701414218 1102 122-123 -0.014389163334524113 1102 124-125 0.23962719874995386 1103 1 0.2424568636043567 1103 2 -0.19004130841351952 1103 3 0.2783071091479563 1103 4 0.16184073812418376 1103 5 0.18687389732422588 1103 6 0.27127492664381236 1103 7 0.49062869035186196 1103 8 0.4838172396716871 1103 9 0.6430939364769586 1103 10-11 0.6037215710194488 1103 12-13 0.6632462419163829 1103 14-15 0.612519634097616 1103 16-17 0.49847911286598645 1103 18-19 0.695091992821574 1103 20-21 0.7139562562921995 1103 22-23 0.6863789566883653 1103 24-25 0.593984089636237 1103 26-27 0.6967646508119856 1103 28-29 0.6751643755163812 1103 30-31 0.6418561630652135 1103 32-33 0.6501776399465626 1103 34-35 0.6378686880039979 1103 36-37 0.8000170967147966 1103 38-39 0.7895603696899371 1103 40-41 0.6148984520401797 1103 42-43 0.5753412447110975 1103 44-45 0.4598959627191448 1103 46-47 0.644680484709582 1103 48-49 0.4137079451620096 1103 50-51 0.3916874522805216 1103 52-53 0.5375694981830108 1103 54-55 0.5711912655175055 1103 56-57 0.611996559193372 1103 58-59 0.5963787491568233 1103 60-61 0.5228489414451047 1103 62-63 0.49166684501462043 1103 64-65 0.4613410762736798 1103 66-67 0.3754660334893991 1103 68-69 0.30173030985201876 1103 70-71 0.24144972567876977 1103 72-73 0.3499733323630494 1103 74-75 0.4857448467432661 1103 76-77 0.4839091304546024 1103 78-79 0.3806745233585893 1103 80-81 0.4492442473591254 1103 82-83 0.46445488664788925 1103 84-85 0.4155642804606927 1103 86-87 0.5433818501383172 1103 88-89 0.6087338108092162 1103 90-91 0.59448188224912 1103 92-93 0.5106565438671602 1103 94-95 0.5749377154265041 1103 96-97 0.6267029756741636 1103 98-99 0.6266852552971827 1103 100-101 0.5883997354171839 1103 102-103 0.30770355614894385 1103 104-105 0.3729152360543182 1103 106-107 0.5356901188160244 1103 108-109 0.5087339740548806 1103 110-111 0.37344066612028115 1103 112-113 0.4184923323160916 1103 114-115 0.4869592891405503 1103 116-117 0.5784586014804489 1103 118-119 0.37455370672735455 1103 120-121 0.4222163723929704 1103 122-123 0.42185812849924886 1103 124-125 0.7052237223965676 1104 1 0.39916190552624187 1104 2 0.5246955730063974 1104 3 0.28384276552332466 1104 4 0.15942280554171617 1104 5 0.41865892058986987 1104 6 0.48686910239871395 1104 7 0.5713127403593319 1104 8 0.47705590108643037 1104 9 0.5269991030166992 1104 10-11 0.6050762891899666 1104 12-13 0.603305690464147 1104 14-15 0.7614829283087161 1104 16-17 0.7211151540263145 1104 18-19 0.6524060498615682 1104 20-21 0.5774393071705362 1104 22-23 0.6809050626718474 1104 24-25 0.801778900140576 1104 26-27 0.760424699128734 1104 28-29 0.8609661383853577 1104 30-31 0.7656413211513424 1104 32-33 0.7762503282306668 1104 34-35 0.8392311492430977 1104 36-37 0.8759353791017759 1104 38-39 0.962043098092245 1104 40-41 0.9449641041523051 1104 42-43 0.934445043733831 1104 44-45 0.8551286920323946 1104 46-47 0.8732046740558417 1104 48-49 0.8113951816699441 1104 50-51 0.630964486521286 1104 52-53 0.682895508699012 1104 54-55 0.24468273356687575 1104 56-57 0.44195230527903107 1104 58-59 0.6070267893406189 1104 60-61 0.6538433063130782 1104 62-63 0.6753177200724707 1104 64-65 0.679462680710369 1104 66-67 0.6522385585313906 1104 68-69 0.2462315469210914 1104 70-71 0.021236809511606225 1104 72-73 -0.00829357758779281 1104 74-75 0.37269681967714163 1104 76-77 0.4486831786037371 1104 78-79 0.5261280667689654 1104 80-81 0.5628917701237448 1104 82-83 0.43246212140679674 1104 84-85 0.5045261262624905 1104 86-87 0.4986958270147852 1104 88-89 0.5555712683134324 1104 90-91 0.5901671256001002 1104 92-93 0.5389572773634903 1104 94-95 0.6064930428398831 1104 96-97 0.5832109792742344 1104 98-99 0.6286951281651056 1104 100-101 0.6782506676826401 1104 102-103 0.5311883742688153 1104 104-105 0.5358036192651561 1104 106-107 0.575750979335119 1104 108-109 0.5900345089642762 1104 110-111 0.653157880145983 1104 112-113 0.6207393252726305 1104 114-115 0.6711358730079979 1104 116-117 0.6517166843331026 1104 118-119 0.6649508441604475 1104 120-121 0.688945727550216 1104 122-123 0.8089666480609701 1104 124-125 1.2394856166608648 1105 1 0.08988807804659515 1105 2 -0.1023959437151305 1105 3 0.14229323553819384 1105 4 0.12127097339158155 1105 5 -0.11642698363819903 1105 6 0.2763543961198067 1105 7 0.40196249164425524 1105 8 0.4821564028311158 1105 9 0.564254140688 1105 10-11 0.47189444404229164 1105 12-13 0.4989609068291898 1105 14-15 0.5891007286499246 1105 16-17 0.5933932654815877 1105 18-19 0.6006637409730899 1105 20-21 0.5911848375799309 1105 22-23 0.5956430714051848 1105 24-25 0.5629904041342186 1105 26-27 0.7054338135880016 1105 28-29 0.6387892837187437 1105 30-31 0.7102782169139275 1105 32-33 0.8454933427257103 1105 34-35 0.7633905336305915 1105 36-37 0.8413085236261821 1105 38-39 0.7205390505172815 1105 40-41 0.4115263080896838 1105 42-43 0.43369287612844687 1105 44-45 0.654597284900106 1105 46-47 0.6799993506363577 1105 48-49 0.5306258817574943 1105 50-51 0.4024624308242082 1105 52-53 0.4955825843238486 1105 54-55 0.4466211291703601 1105 56-57 0.4820627124814223 1105 58-59 0.33057499260564427 1105 60-61 0.31831992601720316 1105 62-63 0.5879907734522831 1105 64-65 0.3226728130408105 1105 66-67 0.45826160714723585 1105 68-69 0.5500145418928568 1105 70-71 0.5264867470407069 1105 72-73 0.45298116133778166 1105 74-75 0.444781900547742 1105 76-77 0.5068898772818713 1105 78-79 0.32693308745177774 1105 80-81 0.2576472707089046 1105 82-83 0.3300253518444194 1105 84-85 0.3616709544809993 1105 86-87 0.4856313642375554 1105 88-89 0.5802470488401319 1105 90-91 0.4065560739137446 1105 92-93 0.4639271205480533 1105 94-95 0.43972144903196053 1105 96-97 0.4583051799972786 1105 98-99 0.43221084656246944 1105 100-101 0.4203746602832119 1105 102-103 0.09410003052965976 1105 104-105 0.2906582822908348 1105 106-107 0.2781846478240766 1105 108-109 0.37027271634608994 1105 110-111 0.22401493594193767 1105 112-113 0.24897361002128093 1105 114-115 0.37482552117548806 1105 116-117 0.0581676361379273 1105 118-119 0.23262201891896694 1105 120-121 0.27310032100398374 1105 122-123 0.2804879178291948 1105 124-125 0.7529068826538641 1106 1 -0.12742939245104878 1106 2 -0.42644223327299 1106 3 0.03362273110061054 1106 4 -0.07386586858153521 1106 5 0.211401046267909 1106 6 0.3590102857172468 1106 7 0.41922655310175827 1106 8 0.43678031863498745 1106 9 0.4965886898939189 1106 10-11 0.4593452580640047 1106 12-13 0.5629829398582373 1106 14-15 0.51658637208579 1106 16-17 0.41965243944884634 1106 18-19 0.49262392659211685 1106 20-21 0.5312827590765892 1106 22-23 0.496417364026172 1106 24-25 0.5023715128047286 1106 26-27 0.6432248817798794 1106 28-29 0.7281501493845752 1106 30-31 0.8219982335440221 1106 32-33 0.716642325701649 1106 34-35 0.7736538662291608 1106 36-37 0.6415723749921227 1106 38-39 0.6201171320232035 1106 40-41 0.6034611461068735 1106 42-43 0.3511922475153142 1106 44-45 0.33998370013628687 1106 46-47 0.3300076767604878 1106 48-49 0.5844581511561628 1106 50-51 0.49891573271396084 1106 52-53 0.44131176264432526 1106 54-55 0.5527379504149366 1106 56-57 0.03294017704186558 1106 58-59 0.44089502961720584 1106 60-61 0.4123262981790745 1106 62-63 0.2880636593459798 1106 64-65 0.3722519745428805 1106 66-67 0.3055154907949138 1106 68-69 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-1.1427481153479988 2216 76-77 -1.317841288196508 2216 78-79 -1.287675534256941 2216 80-81 -1.2249833699039918 2216 82-83 -1.1207279243372525 2216 84-85 -0.9570019121155546 2216 86-87 -1.0812193703969655 2216 88-89 -1.0566447216105388 2216 90-91 -1.0099550442478247 2216 92-93 -1.0861428561478554 2216 94-95 -1.05808745097303 2216 96-97 -1.1275029582360894 2216 98-99 -1.1654520606350616 2216 100-101 -1.1849887434348148 2216 102-103 -1.053765036040808 2216 104-105 -1.099037669368073 2216 106-107 -1.0344494070717936 2216 108-109 -1.0672949855032563 2216 110-111 -1.1187032600926656 2216 112-113 -0.9331322053723028 2216 114-115 -1.0599318795244344 2216 116-117 -1.160142744731779 2216 118-119 -1.116484711244773 2216 120-121 -1.204529925033757 2216 122-123 -1.2084286271096971 2216 124-125 -1.494839932158328 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67048.0 3 23244.0 4 11364.0 5 9116.0 6 9924.0 7 11765.0 8 12953.0 9 14030.0 10 15452.0 11 16678.0 12 18382.0 13 20638.0 14 23427.0 15 20544.0 16 14448.0 17 12652.0 18 12055.0 19 11247.0 20 11124.0 21 11542.0 22 13188.0 23 16290.0 24 21386.0 25 27829.0 26 34049.0 27 38918.0 28 44689.0 29 53179.0 30 61350.0 31 72214.0 32 87788.0 33 109152.0 34 140713.0 35 195820.0 36 314815.0 37 450622.0 38 149171.0 39 485.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22214668920409 18.949116263687806 13.611376497391047 24.217360549717057 2 15.810470382555785 20.33680694171037 38.4366467978103 25.416075877923543 3 19.34638375508365 24.716616550979197 29.176461518906837 26.76053817503032 4 13.091334346000554 16.56785735447655 35.51558985415846 34.82521844536443 5 13.611904055034413 38.226927932065976 33.427201782598104 14.733966230301506 6 32.886429577325835 36.26633616162321 16.58209023026296 14.265144030787996 7 28.808241034378483 32.564365299661674 20.082175399600136 18.545218266359708 8 26.865025080845633 35.446616185279325 18.74710036422408 18.94125836965096 9 26.81436911969112 15.696349258283195 19.08190203657212 38.40737958545357 10-11 25.650285370265514 26.57745194041685 26.399089788617676 21.37317290069996 12-13 26.44226952710767 23.631401221773505 27.3790650261943 22.54726422492453 14-15 23.135378953695927 24.69852566661619 25.517530938975536 26.64856444071234 16-17 22.909311415411132 28.337222993628174 25.82265626935844 22.930809321602254 18-19 22.737099944499114 26.225464834257927 28.74317223239522 22.29426298884774 20-21 23.368952474212566 25.473176891778355 28.833263967247586 22.3246066667615 22-23 23.086935154598446 25.354071576489783 28.843646855789334 22.715346413122433 24-25 22.912910666817787 25.486867059057282 28.773256990461576 22.826965283663355 26-27 22.759122118156778 25.924738825608877 28.652323163817954 22.663815892416387 28-29 22.570597501664533 25.87095986722287 28.912178318544886 22.64626431256771 30-31 22.617103452453115 25.573913717810058 28.890290466027714 22.918692363709113 32-33 22.51787393239361 25.738875625145972 28.71073665701368 23.032513785446735 34-35 23.084388454777265 25.629321646351954 28.489173772570986 22.79711612629979 36-37 23.074499917633762 25.472045724962843 28.424221455510075 23.029232901893323 38-39 22.814943025048056 25.676011143073595 28.633440875954612 22.87560495592374 40-41 22.969351041233136 25.49448421527919 28.69864556867348 22.837519174814194 42-43 22.959714879747587 25.594654408245614 28.3299247324015 23.115705979605295 44-45 23.031940204405927 25.789878451294477 28.049902468279818 23.12827887601977 46-47 23.065253791255962 26.084469673852645 27.566878402195943 23.28339813269545 48-49 23.308062117450124 26.158209252458715 27.248196775923912 23.28553185416725 50-51 23.173390605795785 26.325219148158542 27.116394074126422 23.384996171919255 52-53 23.548507289756163 26.146393483018098 27.016309432746706 23.28878979447903 54-55 23.14803300706514 26.138615724104763 27.139308151137225 23.574043117692863 56-57 23.201920778003203 26.506194940427708 27.03113233479432 23.26075194677477 58-59 23.27895587198116 26.05396386776691 27.10821452842301 23.558865731828917 60-61 23.2943879454373 26.08249655507227 27.39370281435568 23.229412685134754 62-63 23.22367687472669 26.078596203994785 27.36919943229243 23.328527488986097 64-65 23.059936199228737 25.94021706211765 27.271502933991165 23.72834380466245 66-67 23.01741713245271 26.39518999527828 27.174778402700696 23.412614469568314 68-69 23.11605012822978 26.083506057704085 27.319504370916963 23.480939443149172 70-71 22.9203901635899 26.148573090973166 27.33003531882617 23.601001426610765 72-73 23.07521115812436 26.119182798442246 27.175351983741503 23.63025405969189 74-75 23.019882111926904 26.35021673537085 26.96432131761523 23.665579835087016 76-77 23.253824294231475 26.176517959281252 27.0518714572767 23.517786289210573 78-79 23.14424737219582 26.363597151550668 26.98939701031207 23.502758465941444 80-81 22.933155232351115 26.604859018174743 27.02231187433694 23.439673875137203 82-83 23.166341713887682 26.448395372623484 26.7453726923114 23.63989022117744 84-85 23.080029238867137 26.294331505062885 26.725618561266028 23.900020694803953 86-87 23.212824721434632 26.161054214421114 26.92504121753359 23.701079846610664 88-89 23.001258684718064 26.281525050487343 26.817647649958083 23.89956861483651 90-91 22.903389221540397 26.46239075001916 27.016492978679764 23.617727049760678 92-93 23.011893557728886 26.17390318766069 26.995654921172314 23.818548333438113 94-95 22.868561380742637 26.15339117171594 26.99887256910619 23.979174878435234 96-97 23.08595821347304 26.026714245953432 26.933900907232548 23.953426633340975 98-99 22.826288964216864 26.042384147290004 26.810827404964456 24.32049948352867 100-101 22.59019344530323 26.02388027237855 27.165857556728895 24.22006872558933 102-103 23.054516354172065 25.801327128868977 27.004103628198344 24.14005288876061 104-105 22.8522487359421 25.812293998369196 27.102163042934606 24.233294222754097 106-107 22.80335140825132 26.009687678816185 27.035722627328596 24.151238285603892 108-109 22.884810446335628 25.999310325998298 26.654982435797336 24.46089679186873 110-111 22.583512329021072 26.010409817146783 27.04941841123412 24.356659442598023 112-113 22.769079485025177 25.94047788936861 26.980380316350594 24.310062309255624 114-115 22.45126970193517 26.04229999573256 26.878948245094392 24.62748205723788 116-117 22.333524985878434 25.909940434756074 27.054395213856246 24.70213936550924 118-119 22.452990445057587 26.16318793589291 26.647588596474726 24.736233022574776 120-121 22.23677092018386 26.087714653202838 26.913770705892016 24.761743720721288 122-123 22.346378328570886 26.006183000099576 26.770430997416028 24.87700767391351 124-125 22.27549029693367 25.993751423679747 26.658931340059226 25.071826939327362 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 603.0 1 463.5 2 616.0 3 920.0 4 650.0 5 447.0 6 892.0 7 2201.5 8 3589.5 9 3960.5 10 3004.5 11 1787.0 12 1386.0 13 1408.0 14 1570.0 15 1725.0 16 1914.0 17 2198.0 18 2528.5 19 2848.5 20 3228.5 21 3766.0 22 4547.0 23 5430.5 24 6634.0 25 8125.5 26 9973.5 27 12264.5 28 14783.5 29 18078.5 30 21615.0 31 24818.0 32 28203.5 33 31327.5 34 34655.5 35 37572.5 36 41054.5 37 45897.0 38 49529.5 39 50979.0 40 51877.5 41 56036.0 42 60824.5 43 64556.5 44 68380.5 45 71238.0 46 73203.5 47 76164.5 48 83990.5 49 88127.0 50 87616.0 51 87403.0 52 85503.5 53 85774.0 54 85714.5 55 80988.5 56 74245.0 57 67890.0 58 62267.0 59 56079.0 60 48493.0 61 40083.5 62 32398.5 63 25323.5 64 19298.5 65 14871.0 66 10949.5 67 8670.5 68 6591.5 69 4306.0 70 3045.5 71 2413.5 72 2066.0 73 1736.0 74 1333.0 75 1085.5 76 750.0 77 410.0 78 243.0 79 155.0 80 99.0 81 57.5 82 39.5 83 31.0 84 18.5 85 16.0 86 13.0 87 6.5 88 3.5 89 2.0 90 1.0 91 1.0 92 2.0 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09961955516725393 2 4.5886483264511254E-5 3 0.0 4 0.10104203614845378 5 0.0 6 0.0 7 0.0022943241632255628 8 0.006332334690502553 9 0.004129783493806013 10-11 7.800702154966914E-4 12-13 0.0 14-15 4.5886483264511254E-5 16-17 4.1297834938060126E-4 18-19 0.0021337214717997736 20-21 0.0014683674644643601 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.2943241632255627E-5 52-53 0.0 54-55 0.0 56-57 0.007984248088024958 58-59 8.259566987612025E-4 60-61 0.0 62-63 0.0 64-65 3.212053828515788E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 9.406729069224808E-4 76-77 0.0 78-79 0.0 80-81 1.3765944979353376E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0014683674644643601 90-91 0.0 92-93 0.0020190052636384955 94-95 0.0 96-97 0.008787261545153906 98-99 0.0036709186611609003 100-101 0.0020419485052707506 102-103 0.0 104-105 0.0 106-107 0.0012848215314063152 108-109 2.2943241632255627E-5 110-111 9.177296652902251E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.177296652902251E-4 122-123 0.004129783493806013 124-125 0.08420169679037816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2179291.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.607058405094364 #Duplication Level Percentage of deduplicated Percentage of total 1 74.58025510645753 38.48867581145791 2 12.816124535769116 13.228049748887994 3 5.074882222084438 7.8569922970225985 4 2.4202612726875397 4.996102594206956 5 1.4292585166688176 3.6879913872853103 6 0.8724406281784445 2.7014456672029326 7 0.5794701973924182 2.093332662458946 8 0.38880722491569464 1.6052157731637529 9 0.29641019700432814 1.3767172513801103 >10 1.384287988032413 12.547130664980402 >50 0.0951520323929099 3.382316126755535 >100 0.058196736184891504 5.510357364698171 >500 0.003473606940508111 1.1781232384245026 >1k 8.01601601655705E-4 0.6822092428971263 >5k 1.781336892568233E-4 0.6653401691777323 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7709 0.3537388994861173 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6766 0.31046794576768316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4669 0.21424399036200306 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.5886483264511254E-5 0.0 0.0 0.0 4.5886483264511254E-5 8 4.5886483264511254E-5 0.0 0.0 0.0 4.5886483264511254E-5 9 4.5886483264511254E-5 0.0 0.0 4.5886483264511254E-5 4.5886483264511254E-5 10-11 4.5886483264511254E-5 0.0 0.0 9.177296652902251E-5 4.5886483264511254E-5 12-13 9.177296652902251E-5 0.0 0.0 1.3765944979353376E-4 4.5886483264511254E-5 14-15 2.2943241632255627E-4 0.0 0.0 1.6060269142578938E-4 6.882972489676688E-5 16-17 2.2943241632255627E-4 0.0 0.0 1.8354593305804502E-4 9.177296652902251E-5 18-19 2.9826214121932317E-4 0.0 0.0 2.0648917469030066E-4 9.177296652902251E-5 20-21 3.6709186611609003E-4 0.0 0.0 3.441486244838344E-4 9.177296652902251E-5 22-23 4.129783493806013E-4 0.0 0.0 8.030134571289469E-4 9.177296652902251E-5 24-25 6.424107657031576E-4 0.0 0.0 0.001422480981199849 9.177296652902251E-5 26-27 6.653540073354133E-4 0.0 0.0 0.002041948505270751 9.177296652902251E-5 28-29 7.800702154966914E-4 0.0 0.0 0.0029367349289287203 9.177296652902251E-5 30-31 7.800702154966914E-4 0.0 0.0 0.005368718541947817 9.177296652902251E-5 32-33 8.03013457128947E-4 0.0 0.0 0.009406729069224809 9.177296652902251E-5 34-35 8.718431820257139E-4 0.0 0.0 0.014913107060966158 9.177296652902251E-5 36-37 9.636161485547364E-4 0.0 0.0 0.02470987123793931 9.177296652902251E-5 38-39 0.0011471620816127814 0.0 0.0 0.04180258625396975 9.177296652902251E-5 40-41 0.0011471620816127814 0.0 0.0 0.06364455228787712 9.177296652902251E-5 42-43 0.001238935048141804 0.0 0.0 0.08461467513975876 9.177296652902251E-5 44-45 0.0013765944979353378 0.0 0.0 0.10758086001364664 9.177296652902251E-5 46-47 0.0015601404309933827 0.0 0.0 0.1369711525445661 1.1471620816127814E-4 48-49 0.001606026914257894 0.0 0.0 0.17193665279212367 1.3765944979353376E-4 50-51 0.001606026914257894 0.0 0.0 0.21036658252615187 1.3765944979353376E-4 52-53 0.001606026914257894 0.0 0.0 0.24966835544220575 1.3765944979353376E-4 54-55 0.001606026914257894 0.0 0.0 0.28922250401621447 1.3765944979353376E-4 56-57 0.0016519133975224053 0.0 0.0 0.3388946221500479 1.3765944979353376E-4 58-59 0.0016519133975224053 0.0 0.0 0.3943025506919452 1.3765944979353376E-4 60-61 0.0017436863640514278 0.0 0.0 0.45980550555203503 1.3765944979353376E-4 62-63 0.0017436863640514278 0.0 0.0 0.5261114738692538 1.3765944979353376E-4 64-65 0.0017895728473159389 0.0 0.0 0.5903754936812018 1.3765944979353376E-4 66-67 0.001973118780373984 0.0 0.0 0.6651475181607229 1.3765944979353376E-4 68-69 0.002019005263638495 0.0 0.0 0.7579988170464614 1.3765944979353376E-4 70-71 0.0021337214717997736 0.0 0.0 0.863239466413618 1.8354593305804502E-4 72-73 0.0022713809215933072 0.0 0.0 0.9749271666794384 1.8354593305804502E-4 74-75 0.0022943241632255628 0.0 0.0 1.0848482373395751 1.8354593305804502E-4 76-77 0.0022943241632255628 0.0 0.0 1.2051625964591237 2.0648917469030066E-4 78-79 0.0022943241632255628 0.0 0.0 1.346378248705657 2.2943241632255627E-4 80-81 0.0027531889958706756 0.0 0.0 1.5103765398930202 2.2943241632255627E-4 82-83 0.0027531889958706756 0.0 0.0 1.6868330112866983 2.2943241632255627E-4 84-85 0.0027990754791351866 0.0 0.0 1.8619587746657054 2.753188995870675E-4 86-87 0.0028679052040319532 0.0 0.0 2.043829851084596 2.753188995870675E-4 88-89 0.002890848445664209 0.0 0.0 2.252246258072006 2.753188995870675E-4 90-91 0.003510315969735111 0.0 0.0 2.490075900831968 2.753188995870675E-4 92-93 0.003647975419528645 0.0 0.0 2.750665239291127 2.753188995870675E-4 94-95 0.003716805144425412 0.0 0.0 3.0176786854073185 2.753188995870675E-4 96-97 0.003716805144425412 0.0 0.0 3.2856328044304317 2.753188995870675E-4 98-99 0.004175669977070524 0.0 0.0 3.577195519093136 2.753188995870675E-4 100-101 0.0044739321182898474 0.0 0.0 3.917581451949281 2.753188995870675E-4 102-103 0.004680421292980148 0.0 0.0 4.283526155983758 2.753188995870675E-4 104-105 0.005552264475005862 0.0 0.0 4.6574551081062605 2.753188995870675E-4 106-107 0.005689923924799396 0.0 0.0 5.02830966585004 2.753188995870675E-4 108-109 0.005735810408063907 0.0 0.0 5.413113714506231 2.753188995870675E-4 110-111 0.005942299582754208 0.0 0.0 5.847291619155037 2.753188995870675E-4 112-113 0.00603407254928323 0.0 0.0 6.328709658324657 2.753188995870675E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5330 0.0 55.366272 1 GTATCAA 8955 0.0 48.13267 1 ATCAACG 11660 0.0 37.039696 3 TCAACGC 11675 0.0 36.89006 4 CAACGCA 11900 0.0 36.29937 5 TATCAAC 12010 0.0 36.00987 2 AACGCAG 12230 0.0 35.36856 6 GTACATG 10640 0.0 31.2651 1 ACGCAGA 14205 0.0 30.0741 7 TACATGG 10735 0.0 30.020863 2 CGCAGAG 14350 0.0 29.770214 8 ACATGGG 11235 0.0 28.20762 3 GCAGAGT 15390 0.0 27.138636 9 GAGTACT 8805 0.0 26.657358 12-13 GTACTTT 9335 0.0 25.239475 14-15 CATGGGG 5030 0.0 24.988625 4 AGTACTT 9100 0.0 24.845152 12-13 AGAGTAC 14615 0.0 23.488567 10-11 CAGAGTA 15200 0.0 23.210829 10-11 ATTCGCG 445 0.0 22.728376 80-81 >>END_MODULE