FastQCFastQC Report
Thu 26 May 2016
SRR1364564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429985
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32530.22748490368780092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.16279891047808195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20270.14174973863362203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14580.1019591114592111No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48800.052.4283871
GGTATCA27250.051.312031
ATCAACG59100.042.786243
TCAACGC60100.042.1733174
CAACGCA61000.041.8437045
AACGCAG62800.040.6372576
TATCAAC62900.040.201382
GTACATG76350.035.614351
TACATGG76850.034.4523472
ACGCAGA73100.034.1801387
CGCAGAG72400.033.6924638
ACATGGG79950.032.2978743
GCAGAGT77600.029.44139
TACCTGG13350.027.186372
CATGGGG30650.026.7886774
CATGGGA51850.026.1630744
GAGTACT34750.025.51763712-13
AGTACTT37250.024.52398912-13
AGAGTAC72000.024.21536310-11
GAGTACA59200.023.820151
Produced by FastQC (version 0.11.5)