##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364564_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1429985 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.405863697870956 30.0 25.0 31.0 2.0 34.0 2 25.44976136113316 31.0 25.0 31.0 2.0 34.0 3 25.610377731234944 31.0 25.0 31.0 2.0 34.0 4 28.629169536743394 35.0 27.0 37.0 2.0 37.0 5 28.705941670716825 35.0 27.0 37.0 2.0 37.0 6 28.669105619989022 35.0 28.0 37.0 2.0 37.0 7 28.37449273943433 35.0 27.0 37.0 2.0 37.0 8 28.270367870991652 35.0 27.0 37.0 2.0 37.0 9 29.064801379035444 35.0 26.0 39.0 2.0 39.0 10-11 28.9618440053567 35.0 26.5 39.0 2.0 39.0 12-13 28.822324010391718 35.0 25.5 39.0 2.0 39.0 14-15 29.2845928453795 36.0 24.5 40.0 2.0 41.0 16-17 29.370957737318925 36.0 25.0 40.0 2.0 41.0 18-19 29.16713741752536 36.0 24.0 40.0 2.0 41.0 20-21 28.86155099529016 36.0 22.5 40.0 2.0 41.0 22-23 28.735483588988693 36.0 21.5 40.0 2.0 41.0 24-25 28.53840564761169 35.5 20.0 40.0 2.0 41.0 26-27 28.38530089476463 35.0 20.5 40.0 2.0 41.0 28-29 28.412246282303663 35.0 20.5 40.0 2.0 41.0 30-31 28.323734514697705 35.0 21.0 40.0 2.0 41.0 32-33 28.25217292489082 35.0 21.5 40.0 2.0 41.0 34-35 27.78250645985797 35.0 17.5 39.0 2.0 41.0 36-37 27.640402172050756 34.5 17.0 39.0 2.0 41.0 38-39 27.429294363227587 34.0 16.0 39.0 2.0 41.0 40-41 27.603936404927325 34.5 17.5 39.0 2.0 41.0 42-43 27.47622387647423 34.0 16.5 39.0 2.0 41.0 44-45 27.305443064088085 34.0 15.5 39.0 2.0 41.0 46-47 27.230962212890347 34.0 15.0 39.0 2.0 41.0 48-49 27.050873260908332 34.0 14.0 39.0 2.0 40.5 50-51 26.88074455326454 34.0 10.5 39.0 2.0 40.0 52-53 26.807039234677287 34.0 9.0 39.0 2.0 40.0 54-55 26.81322915974643 34.0 8.5 39.0 2.0 41.0 56-57 26.684444242422124 34.0 8.0 39.0 2.0 41.0 58-59 26.483264859421602 33.5 7.5 39.0 2.0 40.0 60-61 26.277401161550646 33.0 6.5 39.0 2.0 40.0 62-63 26.005911950125352 33.0 2.0 38.0 2.0 40.0 64-65 25.743636821365257 33.0 2.0 38.0 2.0 40.0 66-67 25.51812396633531 32.5 2.0 37.5 2.0 40.0 68-69 25.475442399745454 33.0 2.0 37.0 2.0 40.0 70-71 25.260611824599557 32.5 2.0 37.0 2.0 39.5 72-73 24.99911222844995 32.0 2.0 36.0 2.0 39.0 74-75 24.72480305737473 32.0 2.0 36.0 2.0 39.0 76-77 24.44056511082284 32.0 2.0 35.5 2.0 38.5 78-79 24.152254044622847 32.0 2.0 35.0 2.0 37.0 80-81 23.8705783627101 32.0 2.0 35.0 2.0 37.0 82-83 23.64267072731532 31.5 2.0 35.0 2.0 36.5 84-85 23.40899135305615 31.0 2.0 35.0 2.0 36.0 86-87 23.21770717874663 31.0 2.0 35.0 2.0 36.0 88-89 23.024816693881405 31.0 2.0 35.0 2.0 35.0 90-91 22.888303723465633 31.0 2.0 35.0 2.0 35.0 92-93 22.75620128882471 31.0 2.0 35.0 2.0 35.0 94-95 22.62791497812914 31.0 2.0 35.0 2.0 35.0 96-97 22.513468672748317 31.0 2.0 35.0 2.0 35.0 98-99 22.41025814956101 31.0 2.0 35.0 2.0 35.0 100-101 22.307493435245824 31.0 2.0 35.0 2.0 35.0 102-103 21.34450641090641 29.0 2.0 33.5 2.0 35.0 104-105 22.060045035437433 30.0 2.0 34.0 2.0 35.0 106-107 22.17676199400693 31.0 2.0 35.0 2.0 35.0 108-109 22.114854351619073 31.0 2.0 35.0 2.0 35.0 110-111 22.02378906072441 31.0 2.0 35.0 2.0 35.0 112-113 21.92292926149575 31.0 2.0 35.0 2.0 35.0 114-115 21.78813484057525 30.0 2.0 35.0 2.0 35.0 116-117 21.66100203848292 30.0 2.0 35.0 2.0 35.0 118-119 21.495694710084372 30.0 2.0 35.0 2.0 35.0 120-121 21.297069200026574 30.0 2.0 34.0 2.0 35.0 122-123 21.03407937845502 30.0 2.0 34.0 2.0 35.0 124-125 19.771171026269506 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.938586534337233 1101 2 0.9339928683847205 1101 3 0.9613834992683579 1101 4 1.0307023366056924 1101 5 1.053406656603368 1101 6 1.087146607757262 1101 7 1.2540732864324724 1101 8 0.9797850735497811 1101 9 1.4779909544409655 1101 10-11 1.4869347631715968 1101 12-13 1.2257243112829137 1101 14-15 1.4831778989407667 1101 16-17 1.145069696253362 1101 18-19 1.5144953414063345 1101 20-21 2.116085047887257 1101 22-23 1.875281629550031 1101 24-25 1.954762288074285 1101 26-27 2.0767616514324345 1101 28-29 1.7403560950977237 1101 30-31 1.794780550581283 1101 32-33 1.8227314682775422 1101 34-35 2.1165206627504105 1101 36-37 1.9266479593276529 1101 38-39 1.8424165320141306 1101 40-41 1.8213793737116823 1101 42-43 1.7669900212729743 1101 44-45 1.620947393380277 1101 46-47 1.4516792297808543 1101 48-49 1.3397711777165497 1101 50-51 1.4294614605609937 1101 52-53 1.2003987019320377 1101 54-55 1.0255841430705495 1101 56-57 0.9780743638660425 1101 58-59 1.077242478045271 1101 60-61 1.0716448926993856 1101 62-63 1.0516896032549852 1101 64-65 1.1537391326067912 1101 66-67 1.1188577573932363 1101 68-69 0.8645492074107288 1101 70-71 0.7368806078916457 1101 72-73 0.7065767615810294 1101 74-75 0.8170916584287689 1101 76-77 0.9801551039471725 1101 78-79 0.9673807147562492 1101 80-81 1.0378316708049482 1101 82-83 1.0159915990182533 1101 84-85 1.126594595208605 1101 86-87 1.094311703086646 1101 88-89 1.076618860772797 1101 90-91 1.0970702054166601 1101 92-93 0.9235896568522257 1101 94-95 1.014982976867472 1101 96-97 0.9180198223877163 1101 98-99 1.0082156825084816 1101 100-101 1.0493441612585421 1101 102-103 0.9805347733876957 1101 104-105 0.9626175195756979 1101 106-107 0.9764012794410668 1101 108-109 1.14623414134849 1101 110-111 0.922804978236023 1101 112-113 0.898336161801474 1101 114-115 1.0230435015991866 1101 116-117 1.129570037404708 1101 118-119 1.0793480808040385 1101 120-121 1.2108787803918268 1101 122-123 1.346280217831488 1101 124-125 1.6402028492620921 1102 1 -0.41580362830420725 1102 2 -0.032877517203974804 1102 3 0.1550911963971764 1102 4 0.11043616947668866 1102 5 0.20675083164971753 1102 6 -0.7997447353771037 1102 7 -1.3361239093147397 1102 8 -0.40525538715628073 1102 9 -0.1479155055267718 1102 10-11 -0.09477131962330532 1102 12-13 0.0731188019859843 1102 14-15 0.273531691376931 1102 16-17 0.2864285217849414 1102 18-19 0.3013448864300763 1102 20-21 0.5746053722747924 1102 22-23 0.4263797073093656 1102 24-25 0.5838023690429388 1102 26-27 0.2283335685911858 1102 28-29 0.8588176321748762 1102 30-31 0.6088998758634894 1102 32-33 0.5861280664332504 1102 34-35 0.8035864421333265 1102 36-37 1.0016023424714504 1102 38-39 1.0310697622454583 1102 40-41 0.7766242379769324 1102 42-43 0.4909395962842744 1102 44-45 0.6670103510532783 1102 46-47 0.37101112590107377 1102 48-49 -0.09165618726801839 1102 50-51 0.3977701465818235 1102 52-53 0.11739132112357709 1102 54-55 0.09339392882261066 1102 56-57 0.20872749646346023 1102 58-59 0.33182356671732904 1102 60-61 0.15808408285986886 1102 62-63 0.033199000968508585 1102 64-65 0.05819366838793272 1102 66-67 0.10197226554442906 1102 68-69 0.022064419965822424 1102 70-71 -0.001047038184093907 1102 72-73 0.13455004588444552 1102 74-75 -0.2603264061387378 1102 76-77 -0.19659477575390838 1102 78-79 -0.14113521160618703 1102 80-81 -0.38731090506998456 1102 82-83 -0.1822897067655198 1102 84-85 -0.6215487295678876 1102 86-87 -0.2581585135099047 1102 88-89 0.054475535546377074 1102 90-91 -0.4225783935030236 1102 92-93 -0.0557991278913299 1102 94-95 -0.04753646836496728 1102 96-97 0.16161360954174597 1102 98-99 0.17623081441436383 1102 100-101 0.19238019784381422 1102 102-103 0.02955644339093766 1102 104-105 0.3820940668537922 1102 106-107 0.22296592692304884 1102 108-109 -0.22738978858725645 1102 110-111 -0.04795096691531597 1102 112-113 -0.2783134969185248 1102 114-115 -0.12540840481533877 1102 116-117 -0.28338359751241526 1102 118-119 0.026355365328367242 1102 120-121 0.2676016060520894 1102 122-123 0.20615632625191793 1102 124-125 0.35622158147455707 1103 1 0.3984491081712811 1103 2 0.4440207918285779 1103 3 0.16585459441066774 1103 4 0.24980484728354924 1103 5 0.319708451725333 1103 6 0.4461868525645869 1103 7 0.42577369365569595 1103 8 0.6158044269132645 1103 9 0.5965219454680692 1103 10-11 0.7977761775975019 1103 12-13 0.7348646301170056 1103 14-15 1.115283323133255 1103 16-17 0.8706285397159554 1103 18-19 0.9559434039961943 1103 20-21 1.3173596806795302 1103 22-23 1.1497702335754454 1103 24-25 1.2087016781970874 1103 26-27 1.219388366083674 1103 28-29 1.1479620468585026 1103 30-31 1.1703008171731888 1103 32-33 1.0842699481903146 1103 34-35 1.5139542856690476 1103 36-37 1.3446010464653462 1103 38-39 1.4306912262601301 1103 40-41 1.0841539855488875 1103 42-43 0.9443305993418996 1103 44-45 0.7465048470766327 1103 46-47 0.9788331991595278 1103 48-49 0.9787029599549442 1103 50-51 0.9484545705777165 1103 52-53 0.8926008755698653 1103 54-55 0.6669115348636758 1103 56-57 0.7877412086688906 1103 58-59 0.60178042962972 1103 60-61 0.6188652407880575 1103 62-63 0.5236734359306077 1103 64-65 0.7542467012954468 1103 66-67 0.5950155575322569 1103 68-69 0.7111825312690421 1103 70-71 0.8020201458508289 1103 72-73 0.8007733800626191 1103 74-75 0.79206066555588 1103 76-77 0.6715954033896807 1103 78-79 0.6512528684227057 1103 80-81 0.7081943194475038 1103 82-83 0.8004421125911669 1103 84-85 0.8144852362404542 1103 86-87 0.6680946098739398 1103 88-89 0.6572758988204725 1103 90-91 0.6988122773974297 1103 92-93 0.8280084837548891 1103 94-95 0.7296796028407684 1103 96-97 0.8810525833038412 1103 98-99 0.7569351974974374 1103 100-101 0.7929087292551387 1103 102-103 0.6751397225624238 1103 104-105 0.7256977714100223 1103 106-107 0.7615622024282587 1103 108-109 0.9027887892370998 1103 110-111 0.7974334057741572 1103 112-113 0.8670983426139713 1103 114-115 0.9199726828032055 1103 116-117 0.9026660522283052 1103 118-119 1.0175218324193693 1103 120-121 1.00822187306904 1103 122-123 0.9527937481830051 1103 124-125 1.0719318856698443 1104 1 0.871004070737456 1104 2 1.0397138215963402 1104 3 0.9074734840154122 1104 4 1.0234277912542034 1104 5 0.8645857183655181 1104 6 0.9368251792764752 1104 7 0.9821792228980009 1104 8 0.9470969941092768 1104 9 1.2924650916937601 1104 10-11 0.9862662308464394 1104 12-13 1.0405185446047156 1104 14-15 1.1222935328261556 1104 16-17 1.1724923169677552 1104 18-19 1.3264225629597668 1104 20-21 1.6835783667938848 1104 22-23 1.4961949322646788 1104 24-25 2.0587703519149976 1104 26-27 2.091641095660215 1104 28-29 1.892779561787851 1104 30-31 1.6927648653451648 1104 32-33 1.8312074049207254 1104 34-35 2.060744636518397 1104 36-37 1.960335241859017 1104 38-39 2.0313552723731796 1104 40-41 1.714854955045059 1104 42-43 1.8311712394272774 1104 44-45 1.6821189471077744 1104 46-47 1.6005692673167964 1104 48-49 1.6099689541539242 1104 50-51 1.498507673677512 1104 52-53 1.6659039355609266 1104 54-55 1.5458758228145904 1104 56-57 1.573453686696304 1104 58-59 1.584778429899096 1104 60-61 1.6029685190251364 1104 62-63 1.5621705023918402 1104 64-65 1.484217438828015 1104 66-67 1.568938632673575 1104 68-69 1.6439787998111584 1104 70-71 1.453616841962976 1104 72-73 1.4490083454083411 1104 74-75 1.4462081763588017 1104 76-77 1.4933345599340129 1104 78-79 1.5668101464212896 1104 80-81 1.6039703710647437 1104 82-83 1.563707485681192 1104 84-85 1.5922228125600775 1104 86-87 1.5071507172880416 1104 88-89 1.455507282495084 1104 90-91 1.533641905667153 1104 92-93 1.5521614721554222 1104 94-95 1.5629643941644602 1104 96-97 1.4194592501020509 1104 98-99 1.5255286816870388 1104 100-101 1.5423366269986438 1104 102-103 1.4756980128656636 1104 104-105 1.4644860684819783 1104 106-107 1.4313326623491456 1104 108-109 1.5642889132015263 1104 110-111 1.5787465407701262 1104 112-113 1.4864124933461333 1104 114-115 1.484757177727527 1104 116-117 1.6158722097858025 1104 118-119 1.5464991602267801 1104 120-121 1.6256528583417236 1104 122-123 1.706548819357458 1104 124-125 2.0658595590763404 1105 1 -0.1089702544588036 1105 2 0.3260265299520775 1105 3 0.3130085213850222 1105 4 0.4429478673376508 1105 5 0.5071086038908454 1105 6 0.6022856790613993 1105 7 0.7536381835389321 1105 8 0.3583794572773584 1105 9 0.4631496075019115 1105 10-11 0.6796270760986189 1105 12-13 0.5466163104935227 1105 14-15 0.7352420857619535 1105 16-17 0.6111391136663258 1105 18-19 0.8324581581850587 1105 20-21 1.1875656906996817 1105 22-23 1.3624288687094612 1105 24-25 1.3931481243905353 1105 26-27 1.15260321188239 1105 28-29 0.8768201844354486 1105 30-31 0.9914180303360247 1105 32-33 1.229937695140105 1105 34-35 1.624119226528073 1105 36-37 1.7380776402351863 1105 38-39 1.7766735693674782 1105 40-41 1.243720206012906 1105 42-43 1.0267756895025535 1105 44-45 1.195611241306075 1105 46-47 1.3433692323668005 1105 48-49 1.2157905646765599 1105 50-51 1.116163133942056 1105 52-53 1.2647179041328123 1105 54-55 1.0596676283850819 1105 56-57 1.1246965958800885 1105 58-59 1.139790645594335 1105 60-61 1.0519696717028424 1105 62-63 1.11320211533409 1105 64-65 1.0476541995012028 1105 66-67 0.9397005257534765 1105 68-69 0.6867744801262461 1105 70-71 1.0045018497595137 1105 72-73 1.0526859081839106 1105 74-75 1.1138152443230993 1105 76-77 1.132048652175115 1105 78-79 1.1827227277472403 1105 80-81 1.0983687102678878 1105 82-83 1.0392765152811485 1105 84-85 1.0795139527364377 1105 86-87 0.8853890527273371 1105 88-89 0.9525655179236239 1105 90-91 0.9676326237551223 1105 92-93 0.8927264858665716 1105 94-95 0.955236076603434 1105 96-97 0.9815026317828739 1105 98-99 0.9544424089695447 1105 100-101 0.910319323390727 1105 102-103 0.9368300062251649 1105 104-105 0.9793629201595735 1105 106-107 0.9245451381529932 1105 108-109 0.8417263392688419 1105 110-111 0.9326089352480267 1105 112-113 0.9719960406361672 1105 114-115 0.9729846585050268 1105 116-117 0.9974543187491314 1105 118-119 0.911896733218498 1105 120-121 0.8545740629067318 1105 122-123 0.9957562442149701 1105 124-125 1.0578235807453389 1106 1 0.049382046146334346 1106 2 0.3905206489620525 1106 3 0.14987876820942603 1106 4 0.37777867813741395 1106 5 0.1698797393376097 1106 6 0.10392671719583646 1106 7 0.11690431367031451 1106 8 0.25542720710017974 1106 9 0.23455210095990608 1106 10-11 0.47485890840522416 1106 12-13 0.4540597153346049 1106 14-15 0.6652453993556975 1106 16-17 0.5814304884414412 1106 18-19 0.7025039522209298 1106 20-21 0.8969430758057193 1106 22-23 0.9642498971671856 1106 24-25 1.073490037858889 1106 26-27 1.239872751011756 1106 28-29 1.0061627810663154 1106 30-31 0.8557274314018279 1106 32-33 0.8319454859186024 1106 34-35 1.1053497197680038 1106 36-37 1.0292271080158102 1106 38-39 1.1725886067795734 1106 40-41 1.091489322637777 1106 42-43 0.853570779932145 1106 44-45 0.7497269547253644 1106 46-47 0.46425407263284413 1106 48-49 0.7099085098736388 1106 50-51 0.5342100387558801 1106 52-53 0.4031828924798653 1106 54-55 0.36226943833983327 1106 56-57 0.4612022622105698 1106 58-59 0.40869708299552343 1106 60-61 0.33654881122473057 1106 62-63 0.2795532523482116 1106 64-65 0.34732956953336824 1106 66-67 0.2228750081854649 1106 68-69 0.3870550282236138 1106 70-71 0.36571806643617677 1106 72-73 0.2108275954079204 1106 74-75 0.3614224718758443 1106 76-77 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82-83 -2.8546841111241577 2216 84-85 -2.7777132283641173 2216 86-87 -2.8053037761150534 2216 88-89 -2.8751608388798857 2216 90-91 -2.736399594095026 2216 92-93 -2.827446012078351 2216 94-95 -2.827187658920373 2216 96-97 -2.783344820376165 2216 98-99 -2.8621609432221327 2216 100-101 -2.768011883090015 2216 102-103 -2.648760416364958 2216 104-105 -2.8549976678641684 2216 106-107 -2.847059415351513 2216 108-109 -2.8137231632818676 2216 110-111 -2.9041661782344796 2216 112-113 -2.8943691456202103 2216 114-115 -2.9308934097537147 2216 116-117 -2.9718985098514814 2216 118-119 -2.9650350949227473 2216 120-121 -3.0149134033960685 2216 122-123 -2.9643054873981463 2216 124-125 -3.0484663496842934 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 195898.0 3 27549.0 4 16584.0 5 12967.0 6 11319.0 7 11717.0 8 11932.0 9 12204.0 10 12852.0 11 13528.0 12 14412.0 13 15760.0 14 17207.0 15 15012.0 16 11898.0 17 10230.0 18 9387.0 19 8575.0 20 8419.0 21 8889.0 22 10969.0 23 14322.0 24 19020.0 25 23914.0 26 28034.0 27 30212.0 28 34006.0 29 38464.0 30 42678.0 31 48009.0 32 56708.0 33 69076.0 34 84656.0 35 110980.0 36 155289.0 37 171457.0 38 45695.0 39 157.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.866504781374424 18.464784703518415 12.719232294437269 22.94947822066989 2 15.047034001131529 20.131153915280752 41.11815811061155 23.70365397297617 3 19.491441263766852 26.18541084451606 27.972688693628296 26.350459198088792 4 11.875080863146337 16.631337485182375 37.22544120681034 34.26814044486095 5 13.25148431199892 39.17779361541264 33.25656199140152 14.31416008118692 6 31.45482975842816 36.786697324695375 17.693472860931152 14.065000055945308 7 28.613839989761313 33.25132127303792 19.953408384392716 18.18143035280805 8 26.029014290359687 36.33052094952045 19.232789155130998 18.407675604988864 9 26.742567021808533 17.061705170478586 19.138358468332697 37.05736933938019 10-11 24.80040000419585 27.968789882411354 26.47527771270328 20.755532400689518 12-13 25.6410704701096 25.277381974323966 27.071697440263787 22.009850115302644 14-15 22.513770130619527 26.246096277256115 25.236741331374912 26.003392260749447 16-17 22.09308466639782 29.983362124052032 25.49914502233015 22.42440818722 18-19 21.890842487256077 28.182029894460932 28.30990623800176 21.617221380281237 20-21 22.78382657785561 27.19445788794924 28.603275024268243 21.4184405099269 22-23 22.35952306024686 27.308752054267632 28.2790307353404 22.05269415014511 24-25 22.116890319683822 27.181587319793625 28.61379657145415 22.087725789068408 26-27 22.012950110773634 27.47598738911708 28.273557538464765 22.23750496164452 28-29 21.795140761005246 27.49970803726214 28.500108743007758 22.20504245872486 30-31 21.9132003692411 27.401990265461972 28.261664661948586 22.423144703348346 32-33 21.838838091161755 27.41143501364475 28.167631100797703 22.582095794395794 34-35 22.304114495165432 27.027235892757297 28.576548954635054 22.092100657442217 36-37 22.162630749992307 26.974678073166842 28.357641618432428 22.505049558408423 38-39 21.926811094805572 27.213686581917496 28.612268174934762 22.24723414834217 40-41 22.315176281232972 27.16867221266645 28.23783819325289 22.27831331284769 42-43 22.07677992073311 27.29916991466955 28.022950611006863 22.601099553590473 44-45 22.236342799910055 27.426797491448678 27.819523629838656 22.517336078802614 46-47 22.336517061179677 27.748434798267745 27.227079694599897 22.68796844595268 48-49 22.511419038492477 27.69741121828725 27.111070696613808 22.680099046606465 50-51 22.410679818058735 27.86965211845204 27.030719372940432 22.688948690548788 52-53 22.61369874509173 27.836900387066997 26.846575313727065 22.702825554114202 54-55 22.4131153824364 27.6399711556149 27.102006587207057 22.84490687474165 56-57 22.418881428450543 28.10964723028604 26.88083121140937 22.59064012985405 58-59 22.55712919563701 27.644009629488476 26.94151851508672 22.857342659787793 60-61 22.60230096305885 27.6478873337935 27.19310760089879 22.55670410224886 62-63 22.470231329309385 27.71366536397765 27.22611878649167 22.589984520221293 64-65 22.362990344364515 27.498557424977356 27.201404456070534 22.937047774587597 66-67 22.360548592032668 28.01595019131768 26.84388246734242 22.779618749307232 68-69 22.4156285628353 27.721044422217407 27.147143786415235 22.716183228532056 70-71 22.20488657721054 27.74754141352332 27.17006862188033 22.87750338738581 72-73 22.43186876055207 27.69909232949181 27.021137015024333 22.847901894931788 74-75 22.302374606158317 27.87677069226974 26.899697164549686 22.921157537022253 76-77 22.472209497900195 27.638602834473858 27.12321534098658 22.765972326639368 78-79 22.507749301994625 27.785386730817308 26.95515807177436 22.751705895413707 80-81 22.301534407556158 28.033376388963678 26.964305320598935 22.700783882881225 82-83 22.52501645152064 27.981221839227562 26.78083777227179 22.71292393698001 84-85 22.46649440378745 27.810921093577907 26.777973195523032 22.94461130711161 86-87 22.47256113338919 27.63734119585483 27.07877301025382 22.81132466050216 88-89 22.541285449602412 27.60360093191704 26.92980756654527 22.925306051935284 90-91 22.426604694431767 27.9742388554022 26.984711533585486 22.614444916580545 92-93 22.461777528501635 27.799531161926765 26.957769606369038 22.780921703202562 94-95 22.33973125913698 27.624070563712287 27.025594034820198 23.010604142330532 96-97 22.55378495948052 27.67714790999371 26.898477315352498 22.87058981517327 98-99 22.29649332172005 27.613277607280985 26.800394273592165 23.2898347974068 100-101 22.09317370077077 27.618267992757367 27.15032237264025 23.13823593383161 102-103 22.426284191792224 27.519764193330698 27.01982887932391 23.03412273555317 104-105 22.361674013585652 27.53355065807578 27.13784153382829 22.966933794510282 106-107 22.163892100852912 27.620073937916068 27.264020878562583 22.952013082668437 108-109 22.233834620642874 27.759976503250034 26.751189697794032 23.25499917831306 110-111 21.970033647547275 27.726131413200484 27.158322496862695 23.145512442389546 112-113 22.190057937670673 27.689731011164454 27.084025356909336 23.036185694255533 114-115 21.927257978230543 27.849977447315883 27.046157826830353 23.176606747623225 116-117 21.894320569796186 27.73109508141694 27.124585222921922 23.249999125864957 118-119 22.104427668821703 27.86896366045798 26.740455319461393 23.28615335125893 120-121 21.889370790162687 27.871990176113858 27.035652147156892 23.202986886566563 122-123 22.12729504155638 27.743612695238063 26.966821330293673 23.162270932911884 124-125 22.132949272734205 27.776590606486756 26.758638827869063 23.331821292909975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1842.0 1 1827.5 2 1640.5 3 1185.0 4 777.5 5 639.5 6 654.0 7 784.0 8 1045.0 9 1249.0 10 1174.0 11 1096.5 12 1161.0 13 1270.5 14 1464.0 15 1604.0 16 1679.5 17 1795.0 18 1963.0 19 2174.5 20 2442.0 21 2878.5 22 3506.0 23 4236.0 24 5209.0 25 6558.5 26 8201.0 27 10150.0 28 12002.0 29 13837.0 30 16470.5 31 19072.0 32 21435.0 33 24278.0 34 27146.5 35 29690.0 36 32746.0 37 36108.0 38 38512.5 39 39615.5 40 40154.5 41 41623.0 42 43494.5 43 45721.5 44 48095.0 45 49906.0 46 50968.5 47 51598.0 48 53919.0 49 55431.0 50 55075.5 51 54254.0 52 52616.0 53 51220.5 54 49260.5 55 45571.5 56 41289.0 57 37086.5 58 33554.5 59 30275.0 60 26039.5 61 21228.5 62 16776.5 63 12996.5 64 9949.5 65 7703.5 66 5897.0 67 4643.0 68 3513.0 69 2534.5 70 1871.5 71 1344.5 72 1028.0 73 816.0 74 615.0 75 492.5 76 355.0 77 214.0 78 155.5 79 118.0 80 83.5 81 65.0 82 49.5 83 29.5 84 22.0 85 23.5 86 19.0 87 15.5 88 12.5 89 7.5 90 7.0 91 7.0 92 7.0 93 5.0 94 2.5 95 4.0 96 4.5 97 4.0 98 5.0 99 2.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011608513376014434 2 0.004335709815137921 3 0.007063011150466613 4 0.006993080346996646 5 0.01384629908705336 6 0.00118882365898943 7 0.007972111595576177 8 0.0 9 0.0027972321387986585 10-11 0.0 12-13 0.003671367182173239 14-15 0.018182008902191283 16-17 0.02905624884177107 18-19 0.00615391070535705 20-21 0.00930079686150554 22-23 0.0024475781214488264 24-25 0.011608513376014434 26-27 0.018706489928216028 28-29 5.594464277597317E-4 30-31 0.0017482700867491616 32-33 0.009160935254565608 34-35 0.008776315835480792 36-37 0.010979136144784735 38-39 3.496540173498323E-5 40-41 0.02559467407000773 42-43 0.009230866058035574 44-45 0.016468704217177103 46-47 0.004021021199523072 48-49 0.024825435231838097 50-51 0.005104948653307553 52-53 0.0 54-55 0.01706311604667182 56-57 0.005035017849837585 58-59 1.7482700867491616E-4 60-61 0.004335709815137921 62-63 0.026433843711647326 64-65 0.017482700867491617 66-67 0.002342681916243877 68-69 0.02062958702364011 70-71 0.003321713164823407 72-73 0.011958167393364267 74-75 3.496540173498323E-5 76-77 0.006293772312296983 78-79 0.0012237890607244132 80-81 0.003636401780438256 82-83 0.001818200890219128 84-85 0.0 86-87 0.005524533474127351 88-89 0.01706311604667182 90-91 0.021853376084364523 92-93 0.004685363832487754 94-95 0.024825435231838097 96-97 0.016818358234526938 98-99 9.440658468445474E-4 100-101 0.008251834809456043 102-103 0.0 104-105 0.0014335814711343125 106-107 1.048962052049497E-4 108-109 0.0 110-111 0.0010139966503145137 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.993080346996647E-5 122-123 0.0 124-125 0.024440815812753282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1429985.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.54195333199185 #Duplication Level Percentage of deduplicated Percentage of total 1 86.67848494756531 62.01148125006475 2 8.040592545812151 11.504793933481087 3 2.394804175038806 5.139869055896564 4 0.9149527274739189 2.6183002131967106 5 0.5177349640449798 1.8519885318023208 6 0.30862354709954265 1.3247718842249558 7 0.21130734325634343 1.0582138062966753 8 0.15685881829170695 0.8977589006349349 9 0.11496196811759007 0.7402143382220306 >10 0.6024557176207055 7.800981186518398 >50 0.040286634536516104 1.9775037561203839 >100 0.018139303895659483 2.2459603177126435 >500 3.986536233579224E-4 0.1817466244543301 >1k 3.986536233579224E-4 0.6464162013742474 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3253 0.22748490368780092 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2328 0.16279891047808195 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2027 0.14174973863362203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1458 0.1019591114592111 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 6.993080346996647E-5 0.0 9 0.0 0.0 0.0 6.993080346996647E-5 0.0 10-11 0.0 0.0 0.0 6.993080346996647E-5 0.0 12-13 0.0 3.496540173498323E-5 0.0 6.993080346996647E-5 0.0 14-15 0.0 6.993080346996647E-5 0.0 6.993080346996647E-5 0.0 16-17 0.0 6.993080346996647E-5 0.0 1.048962052049497E-4 0.0 18-19 0.0 6.993080346996647E-5 0.0 2.7972321387986586E-4 0.0 20-21 0.0 6.993080346996647E-5 0.0 3.8461941908481557E-4 0.0 22-23 0.0 6.993080346996647E-5 0.0 6.993080346996646E-4 0.0 24-25 0.0 6.993080346996647E-5 0.0 7.692388381696311E-4 0.0 26-27 0.0 6.993080346996647E-5 0.0 8.042042399046144E-4 0.0 28-29 0.0 6.993080346996647E-5 0.0 0.0012937198641943797 0.0 30-31 0.0 6.993080346996647E-5 0.0 0.0029720591474735746 0.0 32-33 0.0 1.048962052049497E-4 0.0 0.006608460927911831 0.0 34-35 0.0 1.3986160693993293E-4 0.0 0.011433686367339518 0.0 36-37 0.0 1.3986160693993293E-4 0.0 0.020664552425375092 0.0 38-39 0.0 1.3986160693993293E-4 0.0 0.03580457137662283 0.0 40-41 0.0 1.7482700867491616E-4 0.0 0.05437119969789893 0.0 42-43 0.0 2.097924104098994E-4 0.0 0.07276300101050011 0.0 44-45 0.0 2.097924104098994E-4 0.0 0.09563037374517915 0.0 46-47 0.0 2.097924104098994E-4 0.0 0.12419710696266045 0.0 48-49 0.0 2.097924104098994E-4 0.0 0.15549114151547044 0.0 50-51 0.0 2.7972321387986586E-4 0.0 0.19409294503089192 0.0 52-53 0.0 2.7972321387986586E-4 0.0 0.23133109787864908 0.0 54-55 0.0 2.7972321387986586E-4 0.0 0.2687790431368161 0.0 56-57 0.0 2.7972321387986586E-4 0.0 0.31524806204260886 0.0 58-59 0.0 2.7972321387986586E-4 0.0 0.3727661478966563 0.0 60-61 0.0 2.7972321387986586E-4 0.0 0.43556400941268614 0.0 62-63 0.0 2.7972321387986586E-4 0.0 0.49941083298076555 0.0 64-65 0.0 2.7972321387986586E-4 0.0 0.565075857439064 0.0 66-67 0.0 2.7972321387986586E-4 0.0 0.6385381664842638 0.0 68-69 0.0 2.7972321387986586E-4 0.0 0.7307419308594146 0.0 70-71 0.0 3.1468861561484906E-4 0.0 0.836302478697329 0.0 72-73 0.0 3.496540173498323E-4 0.0 0.9452197051018019 3.496540173498323E-5 74-75 0.0 3.496540173498323E-4 0.0 1.0499760486998115 6.993080346996647E-5 76-77 0.0 3.496540173498323E-4 0.0 1.1664807672807758 6.993080346996647E-5 78-79 0.0 3.496540173498323E-4 0.0 1.3060976164085638 6.993080346996647E-5 80-81 0.0 3.496540173498323E-4 0.0 1.4701902467508399 6.993080346996647E-5 82-83 0.0 3.496540173498323E-4 0.0 1.641310922841848 6.993080346996647E-5 84-85 0.0 3.496540173498323E-4 0.0 1.8126763567450008 6.993080346996647E-5 86-87 0.0 3.496540173498323E-4 0.0 1.983377448015189 6.993080346996647E-5 88-89 0.0 3.496540173498323E-4 0.0 2.1894285604394454 6.993080346996647E-5 90-91 0.0 3.496540173498323E-4 0.0 2.4247107487141477 6.993080346996647E-5 92-93 0.0 3.496540173498323E-4 0.0 2.6832798945443486 6.993080346996647E-5 94-95 0.0 3.496540173498323E-4 0.0 2.9399259432791256 6.993080346996647E-5 96-97 0.0 3.496540173498323E-4 0.0 3.1940544830889834 6.993080346996647E-5 98-99 0.0 3.496540173498323E-4 0.0 3.4743721087983443 6.993080346996647E-5 100-101 0.0 3.8461941908481557E-4 0.0 3.8049350168008758 6.993080346996647E-5 102-103 0.0 4.895156242897653E-4 0.0 4.1653933432868175 6.993080346996647E-5 104-105 0.0 5.594464277597317E-4 0.0 4.521830648573237 6.993080346996647E-5 106-107 0.0 5.594464277597317E-4 0.0 4.8786875386804756 6.993080346996647E-5 108-109 0.0 5.594464277597317E-4 0.0 5.248656454438333 6.993080346996647E-5 110-111 0.0 5.594464277597317E-4 0.0 5.67404553194614 6.993080346996647E-5 112-113 0.0 5.594464277597317E-4 0.0 6.144854666307689 6.993080346996647E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4880 0.0 52.428387 1 GGTATCA 2725 0.0 51.31203 1 ATCAACG 5910 0.0 42.78624 3 TCAACGC 6010 0.0 42.173317 4 CAACGCA 6100 0.0 41.843704 5 AACGCAG 6280 0.0 40.637257 6 TATCAAC 6290 0.0 40.20138 2 GTACATG 7635 0.0 35.61435 1 TACATGG 7685 0.0 34.452347 2 ACGCAGA 7310 0.0 34.180138 7 CGCAGAG 7240 0.0 33.692463 8 ACATGGG 7995 0.0 32.297874 3 GCAGAGT 7760 0.0 29.4413 9 TACCTGG 1335 0.0 27.18637 2 CATGGGG 3065 0.0 26.788677 4 CATGGGA 5185 0.0 26.163074 4 GAGTACT 3475 0.0 25.517637 12-13 AGTACTT 3725 0.0 24.523989 12-13 AGAGTAC 7200 0.0 24.215363 10-11 GAGTACA 5920 0.0 23.82015 1 >>END_MODULE