FastQCFastQC Report
Thu 26 May 2016
SRR1364564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429985
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56360.394130008356731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51100.35734640573152865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32850.22972268939883983No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.10216890386962102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36900.061.555921
GTATCAA63000.049.7755851
ATCAACG83550.037.6398623
TCAACGC84100.037.3228844
TATCAAC84700.036.7068942
CAACGCA85100.036.705785
AACGCAG87650.035.705786
GTACATG77350.031.9088571
TACATGG78650.031.0488622
ACATGGG79600.030.3790043
ACGCAGA102650.030.3142937
CGCAGAG103150.030.0519878
TACCTGG14700.027.5519372
GAGTACT64450.027.0027312-13
GCAGAGT110750.026.9689929
AGTACTT67000.025.3977912-13
CATGGGG31550.025.2968244
ATTCGCG1551.2732926E-1124.94916580-81
GTACTTT69900.024.93992414-15
CATGGGA52750.024.2759674
Produced by FastQC (version 0.11.5)