##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364564_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1429985 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.955336594439803 31.0 30.0 34.0 26.0 34.0 2 29.812770763329684 31.0 30.0 34.0 26.0 34.0 3 30.130341227355533 31.0 30.0 34.0 26.0 34.0 4 33.67604275569324 35.0 35.0 37.0 28.0 37.0 5 33.083541435749325 35.0 33.0 37.0 26.0 37.0 6 33.09960454130638 35.0 33.0 37.0 26.0 37.0 7 32.887305810900116 35.0 33.0 37.0 26.0 37.0 8 32.81934216093176 35.0 33.0 37.0 25.0 37.0 9 34.0199596499264 38.0 34.0 39.0 25.0 39.0 10-11 33.95471001444071 38.0 33.5 39.0 25.0 39.0 12-13 33.82751777116543 38.0 33.0 39.0 23.0 39.0 14-15 34.787972251457184 39.0 33.5 41.0 23.5 41.0 16-17 34.80290667384622 39.0 34.0 41.0 24.0 41.0 18-19 34.83701996874093 39.0 34.0 41.0 24.0 41.0 20-21 34.86092406563705 39.0 34.0 41.0 24.0 41.0 22-23 34.81885963838781 39.0 34.0 41.0 24.0 41.0 24-25 34.781471134312596 39.0 34.0 41.0 24.0 41.0 26-27 34.66460207624556 39.0 34.0 41.0 24.0 41.0 28-29 34.514541061619525 39.0 33.0 41.0 23.0 41.0 30-31 34.344288926107616 38.0 33.0 40.5 22.5 41.0 32-33 34.12770448641069 38.0 33.0 40.0 21.5 41.0 34-35 33.93385909642409 38.0 33.0 40.0 19.0 41.0 36-37 33.72512823561087 38.0 33.0 40.0 18.0 41.0 38-39 33.55819641464771 38.0 33.0 40.0 16.0 41.0 40-41 33.58103686402305 38.0 33.0 40.0 16.0 41.0 42-43 33.44241582953667 38.0 33.0 40.0 15.0 41.0 44-45 33.25972125581737 38.0 32.0 40.0 11.0 41.0 46-47 32.98798448934779 38.0 32.0 40.0 8.5 41.0 48-49 32.68049350168009 38.0 31.0 40.0 4.5 41.0 50-51 32.49908075958839 38.0 31.0 40.0 2.0 41.0 52-53 32.31953377133327 38.0 31.0 40.0 2.0 41.0 54-55 32.132371668234285 37.0 31.0 40.0 2.0 41.0 56-57 31.91689703038843 37.0 30.5 40.0 2.0 41.0 58-59 31.713096640873857 37.0 30.0 40.0 2.0 41.0 60-61 31.474005321734143 36.0 30.0 40.0 2.0 41.0 62-63 31.29595904852149 36.0 30.0 40.0 2.0 41.0 64-65 31.15336419612793 36.0 30.0 39.0 2.0 41.0 66-67 30.851186900561892 35.0 30.0 39.0 2.0 41.0 68-69 30.563975146592448 35.0 29.0 39.0 2.0 41.0 70-71 30.248085469428 35.0 29.0 38.0 2.0 40.0 72-73 29.9568883589688 35.0 29.0 37.0 2.0 40.0 74-75 29.603602834994774 35.0 29.0 37.0 2.0 39.0 76-77 29.294205183970462 35.0 29.0 36.0 2.0 39.0 78-79 29.013012374255673 34.0 29.0 36.0 2.0 38.5 80-81 28.729630730392277 34.0 29.0 35.5 2.0 37.0 82-83 28.450992492928247 34.0 28.0 35.0 2.0 37.0 84-85 28.223789060724414 34.0 28.0 35.0 2.0 36.5 86-87 28.010269338489564 34.0 28.0 35.0 2.0 36.0 88-89 27.8323720178883 34.0 27.0 35.0 2.0 36.0 90-91 27.689780662034917 34.0 27.0 35.0 2.0 36.0 92-93 27.575810935079737 34.0 27.0 35.0 2.0 35.0 94-95 27.460353779934756 34.0 27.0 35.0 2.0 35.0 96-97 27.339647968335335 34.0 27.0 35.0 2.0 35.0 98-99 27.25213726018105 34.0 26.0 35.0 2.0 35.0 100-101 27.150734448263442 34.0 26.0 35.0 2.0 35.0 102-103 25.886800211191026 31.5 24.0 34.0 2.0 35.0 104-105 26.81186131323056 33.5 25.0 35.0 2.0 35.0 106-107 26.969207019654053 34.0 25.0 35.0 2.0 35.0 108-109 26.970027657632773 34.0 25.0 35.0 2.0 35.0 110-111 26.892122644643123 34.0 25.0 35.0 2.0 35.0 112-113 26.808732609083314 34.0 25.0 35.0 2.0 35.0 114-115 26.71078367954909 34.0 25.0 35.0 2.0 35.0 116-117 26.58592083133739 34.0 25.0 35.0 2.0 35.0 118-119 26.45017954733791 34.0 25.0 35.0 2.0 35.0 120-121 26.25304496201009 34.0 25.0 35.0 2.0 35.0 122-123 25.95759431042983 34.0 24.0 35.0 2.0 35.0 124-125 24.187858963555563 31.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5928804905308667 1101 2 0.7371288598585544 1101 3 0.6149572732620499 1101 4 0.6056538560773248 1101 5 0.6168659530359619 1101 6 0.7312335399588434 1101 7 0.7132082775326793 1101 8 0.7713315639611338 1101 9 0.971713818246208 1101 10-11 0.8041723760154582 1101 12-13 0.8099180569656568 1101 14-15 1.0184187661856896 1101 16-17 0.9594955449077105 1101 18-19 0.9728119403354327 1101 20-21 1.068817452755475 1101 22-23 0.9855487461637864 1101 24-25 0.9748418572110467 1101 26-27 0.9200408531359088 1101 28-29 1.0583570882945494 1101 30-31 0.9908003848899583 1101 32-33 1.1583982543307414 1101 34-35 1.1989275204502263 1101 36-37 1.250076203342097 1101 38-39 1.1440095857824772 1101 40-41 0.8978311491300701 1101 42-43 1.0112779361448858 1101 44-45 0.9868456058096484 1101 46-47 1.0404820898795961 1101 48-49 1.135659241510318 1101 50-51 1.1618419250172138 1101 52-53 1.018773413744853 1101 54-55 1.218434411769195 1101 56-57 1.2237589125975035 1101 58-59 1.2363215068606728 1101 60-61 1.184197543050093 1101 62-63 1.0896539484007874 1101 64-65 1.1463164665396874 1101 66-67 1.14692345520481 1101 68-69 1.180974474191622 1101 70-71 1.2248615210116824 1101 72-73 1.1043232346229068 1101 74-75 1.0356072155868432 1101 76-77 1.0320220870468226 1101 78-79 1.0145887271619998 1101 80-81 0.8899150500545545 1101 82-83 1.0599123752043766 1101 84-85 0.9949992226569435 1101 86-87 1.075432948693745 1101 88-89 1.0669473450749116 1101 90-91 1.0855694522651724 1101 92-93 1.043696546549679 1101 94-95 1.023460048009298 1101 96-97 1.0665189019374886 1101 98-99 1.0463131942947044 1101 100-101 1.0628898503391433 1101 102-103 0.9735440323517466 1101 104-105 0.9875462635648979 1101 106-107 0.9434325863629525 1101 108-109 0.9102386734206576 1101 110-111 0.9011775406410614 1101 112-113 0.9710923504379672 1101 114-115 0.9615140902109047 1101 116-117 0.9308346539909174 1101 118-119 0.9162605423431494 1101 120-121 0.9412202939394732 1101 122-123 0.9519989005611968 1101 124-125 1.2704724388515594 1102 1 0.039531771233527024 1102 2 -0.8653490617180211 1102 3 -0.09306920127428597 1102 4 -0.0470418633507208 1102 5 0.18248977408965317 1102 6 0.16273559263301252 1102 7 0.23112511189045648 1102 8 0.2133994501217984 1102 9 0.5938258286341025 1102 10-11 0.5575713970218246 1102 12-13 0.7075828743621315 1102 14-15 0.8132855397697369 1102 16-17 0.8138866074008391 1102 18-19 0.9034366376197056 1102 20-21 0.7038397477057146 1102 22-23 0.8576839839732173 1102 24-25 0.8337083320443313 1102 26-27 0.8968745090285779 1102 28-29 0.5564018230711341 1102 30-31 0.8868684154768189 1102 32-33 1.0245736619691783 1102 34-35 0.9160102147951363 1102 36-37 0.5785567115423333 1102 38-39 0.041198381639205195 1102 40-41 -0.32934675627167564 1102 42-43 0.008340257847713417 1102 44-45 -0.3281562490096377 1102 46-47 -0.15252417286314568 1102 48-49 -0.5246629458851402 1102 50-51 -0.5680934633823931 1102 52-53 -0.665527233900896 1102 54-55 -0.6486424222916334 1102 56-57 -0.8120135662036816 1102 58-59 -0.6971222007802176 1102 60-61 -0.8923864714897114 1102 62-63 -0.7376683382033207 1102 64-65 -0.6718975213397655 1102 66-67 -0.6624884084847587 1102 68-69 -0.4658251920533871 1102 70-71 -1.1156753125047345 1102 72-73 -0.9371839989473223 1102 74-75 -0.7091815746008159 1102 76-77 -1.0276266063572876 1102 78-79 -0.84293989318315 1102 80-81 -0.8968472093560393 1102 82-83 -1.0755779551019309 1102 84-85 -1.0117126585572365 1102 86-87 -0.8422815765096381 1102 88-89 -0.8620962003227213 1102 90-91 -0.7195210986847691 1102 92-93 -0.5674815910137987 1102 94-95 -0.3576984525408484 1102 96-97 -0.8408626264021031 1102 98-99 -0.8732177940101451 1102 100-101 -0.6926865867746095 1102 102-103 -0.7393469278957276 1102 104-105 -0.47606272208404476 1102 106-107 -0.46944084927302043 1102 108-109 -0.4471790402046949 1102 110-111 -0.33693041321544825 1102 112-113 -0.31177861285550534 1102 114-115 -0.22359462424523358 1102 116-117 -0.30160648389852796 1102 118-119 -0.1415410930023029 1102 120-121 -0.186390822558252 1102 122-123 -0.288182649014896 1102 124-125 -0.12927261067065388 1103 1 0.14236452895703522 1103 2 -0.09832629184203157 1103 3 0.11081370693512937 1103 4 0.09672949388773588 1103 5 0.022402564581746276 1103 6 0.25143889529275754 1103 7 0.4325195788728635 1103 8 0.39182397116872636 1103 9 0.5200413105802397 1103 10-11 0.6235432635831586 1103 12-13 0.4825929291885913 1103 14-15 0.5898651284834244 1103 16-17 0.5508370623717553 1103 18-19 0.5619893311839519 1103 20-21 0.6093014518773145 1103 22-23 0.6105824317087638 1103 24-25 0.4618994419580176 1103 26-27 0.47015006921871816 1103 28-29 0.5275316388083127 1103 30-31 0.5060620811300609 1103 32-33 0.7072092333103157 1103 34-35 0.6715395708961367 1103 36-37 0.5745651222364287 1103 38-39 0.5936338168780182 1103 40-41 0.45732313311040684 1103 42-43 0.6300634281145463 1103 44-45 0.5243651683980914 1103 46-47 0.6626691432950409 1103 48-49 0.5848232211146396 1103 50-51 0.4914979513584967 1103 52-53 0.5535386333680989 1103 54-55 0.6319667393605499 1103 56-57 0.5197127819130003 1103 58-59 0.7626913440691006 1103 60-61 0.6536548241331381 1103 62-63 0.5717765600929354 1103 64-65 0.5464717512018105 1103 66-67 0.43915351102780775 1103 68-69 0.48031025278969963 1103 70-71 0.5074684228079747 1103 72-73 0.40485290928080175 1103 74-75 0.456929291524105 1103 76-77 0.429113914331019 1103 78-79 0.4019876949565564 1103 80-81 0.47040227603988427 1103 82-83 0.38663642908300844 1103 84-85 0.5511069086939813 1103 86-87 0.5496452932489362 1103 88-89 0.5377750659603393 1103 90-91 0.4226352127463393 1103 92-93 0.34784319642044537 1103 94-95 0.34064911115702046 1103 96-97 0.4703852967600852 1103 98-99 0.44922868499836355 1103 100-101 0.3734490770732073 1103 102-103 0.15313874611527467 1103 104-105 0.3242728342233292 1103 106-107 0.3437077383632072 1103 108-109 0.2885630105510195 1103 110-111 0.26768278451137917 1103 112-113 0.16529980475320372 1103 114-115 0.29844033119072577 1103 116-117 0.3016792257919967 1103 118-119 0.1560904392984952 1103 120-121 0.08384527138291276 1103 122-123 0.2689113133669352 1103 124-125 0.5970468757085072 1104 1 0.4547381517294298 1104 2 0.5634631539383328 1104 3 0.34192114248435246 1104 4 0.2278503982361002 1104 5 0.5898647339849248 1104 6 0.5890937543121808 1104 7 0.6410112277884537 1104 8 0.6203067066560948 1104 9 0.6296492398424505 1104 10-11 0.6920967703179883 1104 12-13 0.8076222903128354 1104 14-15 0.8823461070217036 1104 16-17 0.8368795045309838 1104 18-19 0.8007217584760298 1104 20-21 0.6195970405453437 1104 22-23 0.7890441275592508 1104 24-25 0.8913968198316127 1104 26-27 0.7713413716344419 1104 28-29 0.975642339468969 1104 30-31 1.0136886814078352 1104 32-33 1.044817552052443 1104 34-35 1.131709672392546 1104 36-37 1.0611194859986668 1104 38-39 1.135607249028304 1104 40-41 1.166706982733409 1104 42-43 1.0669823716802753 1104 44-45 0.9763068630818594 1104 46-47 1.137331930032481 1104 48-49 1.0787343802291929 1104 50-51 0.9950927052183403 1104 52-53 0.9902650889206299 1104 54-55 0.5664629076158718 1104 56-57 0.6444353632555391 1104 58-59 0.7709994269785589 1104 60-61 0.8815366109709437 1104 62-63 0.8279935475944029 1104 64-65 0.8999234955828825 1104 66-67 0.944195839088291 1104 68-69 0.8059991132349253 1104 70-71 0.3256102518365829 1104 72-73 0.3216285988704506 1104 74-75 0.7320406664826038 1104 76-77 0.9273683322258179 1104 78-79 0.9589477398067991 1104 80-81 0.8716488477157682 1104 82-83 0.8272688363863558 1104 84-85 0.8749311554492465 1104 86-87 0.8914330146798548 1104 88-89 0.8914672326954935 1104 90-91 0.9638562794923935 1104 92-93 0.9067573967383744 1104 94-95 0.9771680442565511 1104 96-97 1.1018363797115924 1104 98-99 0.9985519421829423 1104 100-101 0.968981467819372 1104 102-103 0.8627374960048222 1104 104-105 0.8639366246865592 1104 106-107 0.8380680004300061 1104 108-109 0.9447961477431797 1104 110-111 0.8820183051662553 1104 112-113 0.8665354598387047 1104 114-115 0.9881179121600852 1104 116-117 0.9081916904364 1104 118-119 0.9293685133803251 1104 120-121 0.817755739899809 1104 122-123 0.9573205109267668 1104 124-125 1.2979691881897928 1105 1 0.1349415002077592 1105 2 0.06418651800973763 1105 3 0.31333271899795534 1105 4 0.21859453066969792 1105 5 0.01640673446885188 1105 6 0.3384123974458504 1105 7 0.5627366821383859 1105 8 0.5465454151193683 1105 9 0.7891573186681313 1105 10-11 0.7760061624569232 1105 12-13 0.7434037755531833 1105 14-15 0.9078369192058062 1105 16-17 0.767710440653147 1105 18-19 0.7244118898073566 1105 20-21 0.8030152004281206 1105 22-23 0.8164433270699547 1105 24-25 0.8398649010769077 1105 26-27 0.901593532485073 1105 28-29 0.9587911491528089 1105 30-31 0.9884973805959234 1105 32-33 0.9945618121879463 1105 34-35 1.0186342576974141 1105 36-37 1.0509755861169552 1105 38-39 1.0746968016683738 1105 40-41 0.850547735099255 1105 42-43 0.8504020438224345 1105 44-45 1.0500454707278521 1105 46-47 0.9037907730532311 1105 48-49 0.8431113461663955 1105 50-51 0.6950457411630566 1105 52-53 0.7967870435662903 1105 54-55 0.7836372173534798 1105 56-57 0.7331390377097833 1105 58-59 0.5006381146062715 1105 60-61 0.6054030885491848 1105 62-63 0.7854482302215686 1105 64-65 0.7273755401575634 1105 66-67 0.814721651675665 1105 68-69 0.9669614815003342 1105 70-71 1.0059425624344982 1105 72-73 0.8690454918159247 1105 74-75 0.787827495039437 1105 76-77 0.8961758820967702 1105 78-79 0.9162322932534082 1105 80-81 0.8337297838379314 1105 82-83 0.8206548019471711 1105 84-85 0.6842075225317288 1105 86-87 0.8310518581038764 1105 88-89 0.9043016940623083 1105 90-91 0.8004621141251498 1105 92-93 0.9519831938670968 1105 94-95 0.8349421496711109 1105 96-97 0.9079391024505945 1105 98-99 0.8351199703311281 1105 100-101 0.8449896407411899 1105 102-103 0.5726441391922634 1105 104-105 0.7469524395591272 1105 106-107 0.7305450981017998 1105 108-109 0.6755858327899595 1105 110-111 0.602229315758791 1105 112-113 0.599342330873224 1105 114-115 0.6044392235914202 1105 116-117 0.47971567823078587 1105 118-119 0.6289035776345457 1105 120-121 0.7806196903245848 1105 122-123 0.8001513932797089 1105 124-125 1.2943855535247764 1106 1 -0.1347863080842302 1106 2 -0.5327207661040951 1106 3 -0.08325078697162525 1106 4 -0.16781777579598867 1106 5 0.025633261618168035 1106 6 0.2032241614702741 1106 7 0.25717734070023823 1106 8 0.24622819601671608 1106 9 0.35032762205361934 1106 10-11 0.366896164773884 1106 12-13 0.5191150056545837 1106 14-15 0.37640079575272267 1106 16-17 0.3146229208908409 1106 18-19 0.40631689955221617 1106 20-21 0.34911269845188997 1106 22-23 0.3743321930542578 1106 24-25 0.3250930023962084 1106 26-27 0.5673662812857074 1106 28-29 0.6850165399694745 1106 30-31 0.49293303895333196 1106 32-33 0.5511237499843773 1106 34-35 0.44803022107581825 1106 36-37 0.4115908412551619 1106 38-39 0.2757356857113322 1106 40-41 0.2586178691422134 1106 42-43 0.1551672727244764 1106 44-45 0.265064057518444 1106 46-47 -0.05646505512373068 1106 48-49 0.20846593476967712 1106 50-51 0.20454336116796412 1106 52-53 0.06706278737863869 1106 54-55 0.09875125097666171 1106 56-57 -0.3276230292231048 1106 58-59 0.07231289347286562 1106 60-61 0.17200632275296712 1106 62-63 0.11578477720134117 1106 64-65 0.23858851990460295 1106 66-67 0.2494138281871443 1106 68-69 0.3352555003358546 1106 70-71 0.21544239718072333 1106 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-1.114518598871232 2216 84-85 -1.1176848473940488 2216 86-87 -1.409039577668949 2216 88-89 -1.3633556212528468 2216 90-91 -1.3555471510945019 2216 92-93 -1.3975574739721708 2216 94-95 -1.1750456520926278 2216 96-97 -1.2541536033894474 2216 98-99 -1.3000727723793943 2216 100-101 -1.467430271736749 2216 102-103 -1.40594415956744 2216 104-105 -1.255288311087618 2216 106-107 -1.338459274223581 2216 108-109 -1.3999804275458665 2216 110-111 -1.3990439625105964 2216 112-113 -1.3507031716835307 2216 114-115 -1.4769874978369764 2216 116-117 -1.4419887580135047 2216 118-119 -1.4900428256801703 2216 120-121 -1.5499154907638797 2216 122-123 -1.554187914884988 2216 124-125 -1.7498453304903556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47680.0 3 15546.0 4 7375.0 5 5755.0 6 6350.0 7 7618.0 8 8607.0 9 9115.0 10 10059.0 11 11154.0 12 12404.0 13 14327.0 14 17097.0 15 14992.0 16 9953.0 17 8822.0 18 8156.0 19 7756.0 20 7516.0 21 7627.0 22 8622.0 23 10558.0 24 13605.0 25 18069.0 26 22795.0 27 25195.0 28 28705.0 29 34254.0 30 39809.0 31 46299.0 32 56134.0 33 69646.0 34 89764.0 35 123036.0 36 195638.0 37 300742.0 38 108681.0 39 524.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.530757400865056 19.348053280768426 13.676279222138302 23.44491009622822 2 15.506596223037306 20.64692986290066 39.2716706818603 24.574803232201738 3 19.445238936072755 25.535792333486018 29.184012419710697 25.834956310730533 4 12.665727717934574 16.9486079571705 36.15888300568129 34.226781319213636 5 12.80859589436253 39.0111085081312 33.70671720332731 14.47357839417896 6 31.710752210687527 37.02570306681538 17.066053140417555 14.197491582079532 7 28.207850188083107 33.07703120312346 20.320458084986008 18.394660523807428 8 26.535491992447025 36.13280648996433 18.7746695573117 18.557031960276944 9 26.645971029371346 15.992519918793285 19.357507460150174 38.004001591685196 10-11 25.19073746692252 27.341333001393032 26.49918600032448 20.96874353135997 12-13 26.081427427560428 24.468788134141267 27.569904579418665 21.87987985887964 14-15 22.809311153131784 25.36661249356636 25.98487115939759 25.83920519390427 16-17 22.07477654415567 29.125327233483254 26.36952042353025 22.430375798830823 18-19 21.84941678173451 26.862446925721713 29.621487691732636 21.666648600811143 20-21 22.709441681162968 25.95375752860652 29.84032798062886 21.49647280960165 22-23 22.353276432969576 26.018244946625313 29.633073074193085 21.995405546212023 24-25 22.074567215740025 26.212652580271822 29.792270548292464 21.92050965569569 26-27 21.95124424382074 26.489333804200744 29.59275796599265 21.966663985985868 28-29 21.751941453931334 26.46964828302395 29.89370517872565 21.884705084319066 30-31 21.887292524047457 26.183771158438724 29.911222844994878 22.017713472518942 32-33 21.796102756322618 26.30066049643877 29.733563638779426 22.16967310845918 34-35 22.15086172232576 26.290415633730426 29.595520232729715 21.9632024112141 36-37 22.08736455277503 26.20926093630353 29.457686619090413 22.245687891831032 38-39 21.94641901838131 26.43720039021388 29.642758490473675 21.973622100931127 40-41 22.126665664325152 26.17653332027958 29.650590740462313 22.046210274932953 42-43 22.05488169456323 26.233142305688524 29.40688189036948 22.30509410937877 44-45 22.199113976720035 26.44615153305804 29.076423878572154 22.278310611649772 46-47 22.299429714297702 26.76891715647367 28.492291877187526 22.439361252041103 48-49 22.48499110130526 26.917415217642144 28.1343510596265 22.4632426214261 50-51 22.39206089296772 27.073754995246453 27.99638737342957 22.537796738356256 52-53 22.617789697094725 27.11801172739574 27.779417266614686 22.48478130889485 54-55 22.37271719633423 26.92909366182163 28.042916534089517 22.655272607754625 56-57 22.397380482228773 27.339678148154235 27.813423699796104 22.44951766982089 58-59 22.473621861127352 26.924408310092783 27.91953269015058 22.68243713862929 60-61 22.495760445039632 26.93388392185932 28.20438675930167 22.365968873799375 62-63 22.41013017619066 26.95234565397539 28.175295545058166 22.462228624775786 64-65 22.253135619681526 26.79393180536378 28.15377552351238 22.799157051442307 66-67 22.22827512176701 27.278153267341963 27.8686839372441 22.624887673646928 68-69 22.28503176083377 27.019593569021204 28.039115109289646 22.65625956085538 70-71 22.071140606369998 27.04825575093445 28.06746224610748 22.813141396588076 72-73 22.36285695304496 26.983115207502177 27.861865683905773 22.792162155547086 74-75 22.264697994462104 27.154221552897607 27.693884922398475 22.887195530241815 76-77 22.407717563470943 26.982101210851862 27.873229439469643 22.73695178620755 78-79 22.375829117088642 27.139270691650612 27.70039545869362 22.78450473256712 80-81 22.157279657114444 27.349165006680153 27.717386553602267 22.776168782603133 82-83 22.34439522092889 27.31518162777931 27.527316720105453 22.81310643118634 84-85 22.296457655150228 27.15371140256716 27.494589104081513 23.0552418382011 86-87 22.27859033486365 26.98825512155722 27.811690332416074 22.92146421116305 88-89 22.34401623257081 26.966753370450803 27.664707760227614 23.02452263675077 90-91 22.167120634132527 27.291579981608198 27.754906519998464 22.78639286426081 92-93 22.2342661132693 27.127217290054585 27.700626942805894 22.93788965387023 94-95 22.166840910918644 26.947870082553315 27.67182872547614 23.213460281051898 96-97 22.323358067516626 26.950387098127806 27.56030268485946 23.165952149496107 98-99 22.081158102697692 26.89613091595713 27.562319702082277 23.4603912792629 100-101 21.922510468912158 26.999443336401047 27.822305426491035 23.255740768195768 102-103 22.300059091528933 26.887484833756996 27.538086063839827 23.27437001087424 104-105 22.245862718839707 26.808952541460222 27.667842669678354 23.277342070021714 106-107 21.967172531278827 26.963567537855926 27.713972618833658 23.355287312031585 108-109 22.053238320681686 27.012171456343946 27.286614894561833 23.647975328412535 110-111 21.812644560335528 26.972637777035736 27.661702153697686 23.553015508931054 112-113 21.97855222257576 27.041472462997863 27.486931681101552 23.493043633324824 114-115 21.807431546484754 27.115144564453473 27.397979699087756 23.67944418997402 116-117 21.717570464025844 26.951646345940695 27.507246579509577 23.823536610523888 118-119 21.86550907876656 27.137592352367335 27.16906121392882 23.82783735493729 120-121 21.615997806948535 27.090654285996006 27.45457775017815 23.838770156877306 122-123 21.786155121108596 27.09577142697293 27.302671837137172 23.815401614781297 124-125 21.635596456004702 27.04362941402711 27.22326491459352 24.09750921537467 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 381.0 1 279.0 2 380.0 3 650.5 4 494.5 5 314.0 6 636.0 7 1548.0 8 2492.0 9 2776.0 10 2150.5 11 1324.5 12 1090.5 13 1157.5 14 1283.5 15 1449.0 16 1691.0 17 1911.5 18 2140.5 19 2454.0 20 2822.5 21 3152.5 22 3620.0 23 4415.0 24 5362.0 25 6627.0 26 8398.5 27 10405.0 28 12176.5 29 14198.0 30 16826.5 31 19344.0 32 21907.5 33 24406.5 34 27179.0 35 29706.5 36 32706.5 37 36359.0 38 38575.5 39 39473.0 40 39609.0 41 40950.5 42 43021.0 43 45080.0 44 47772.0 45 49940.5 46 50921.5 47 51514.5 48 54696.5 49 56670.0 50 56220.5 51 55261.0 52 52852.0 53 51677.0 54 50290.5 55 46278.5 56 41537.0 57 37261.0 58 33628.0 59 30338.0 60 26232.0 61 21047.0 62 16065.0 63 12132.0 64 9039.0 65 6732.0 66 5008.5 67 3990.5 68 2970.0 69 1942.0 70 1365.5 71 1033.5 72 782.0 73 610.5 74 493.5 75 373.5 76 236.0 77 131.0 78 76.5 79 44.5 80 29.0 81 17.5 82 12.5 83 6.5 84 5.0 85 5.5 86 3.5 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09804298646489298 2 0.0 3 0.0 4 0.10049056458634183 5 0.0 6 0.0 7 0.002237785711038927 8 0.00594411829494715 9 0.0037762633873781896 10-11 6.293772312296981E-4 12-13 0.0 14-15 6.993080346996647E-5 16-17 3.1468861561484906E-4 18-19 0.002202820309303944 20-21 0.0015734430780742457 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.496540173498323E-5 52-53 0.0 54-55 0.0 56-57 0.007237838159141529 58-59 9.09100445109564E-4 60-61 0.0 62-63 0.0 64-65 3.1468861561484906E-4 66-67 0.0 68-69 3.496540173498323E-5 70-71 0.0 72-73 0.0 74-75 0.0013636506676643462 76-77 0.0 78-79 0.0 80-81 2.4475781214488266E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0014335814711343125 90-91 0.0 92-93 0.002097924104098994 94-95 0.0 96-97 0.008042042399046144 98-99 0.004195848208197988 100-101 0.0025874397283887594 102-103 0.0 104-105 0.0 106-107 0.0016783392832791953 108-109 0.0 110-111 1.7482700867491616E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.790312485795307E-4 122-123 0.004895156242897653 124-125 0.0880079161669528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1429985.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.525154925424985 #Duplication Level Percentage of deduplicated Percentage of total 1 80.9425266327724 49.800014911366596 2 11.149663732312675 13.719695769938589 3 3.4261220561683734 6.323780708975245 4 1.4309953655416354 3.521688462500571 5 0.8002030784016255 2.4616309185231002 6 0.4686291651267386 1.7299489192197097 7 0.3215671352521566 1.3849127470719815 8 0.2326117990200641 1.1449181577753011 9 0.18150387292033784 1.005034851088959 >10 0.9433484982618519 10.711399990173435 >50 0.06750384143682814 2.892773517792052 >100 0.03384349572466288 3.767108833265097 >500 9.115858834458898E-4 0.3759829206209704 >1k 3.4184470629220864E-4 0.4077413138787179 >5k 2.2789647086147245E-4 0.7533679778096117 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5636 0.394130008356731 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5110 0.35734640573152865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3285 0.22972268939883983 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1461 0.10216890386962102 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.496540173498323E-5 0.0 18-19 0.0 0.0 0.0 1.048962052049497E-4 0.0 20-21 0.0 0.0 0.0 2.097924104098994E-4 0.0 22-23 0.0 0.0 0.0 4.895156242897653E-4 0.0 24-25 0.0 0.0 0.0 5.594464277597317E-4 0.0 26-27 0.0 0.0 0.0 5.594464277597317E-4 0.0 28-29 0.0 0.0 0.0 0.0010839274537844802 0.0 30-31 0.0 3.496540173498323E-5 0.0 0.002832197540533642 0.0 32-33 0.0 6.993080346996647E-5 0.0 0.00681825333832173 0.0 34-35 0.0 6.993080346996647E-5 0.0 0.01255257922285898 0.0 36-37 0.0 6.993080346996647E-5 0.0 0.022517718717329202 0.0 38-39 0.0 1.048962052049497E-4 0.0 0.039196215344916206 0.0 40-41 0.0 1.3986160693993293E-4 0.0 0.059266355940796586 0.0 42-43 0.0 1.3986160693993293E-4 0.0 0.07909173872453207 0.0 44-45 0.0 1.3986160693993293E-4 0.0 0.10286821190432067 0.0 46-47 0.0 1.3986160693993293E-4 0.0 0.13255383797732143 0.0 48-49 0.0 1.3986160693993293E-4 0.0 0.1653863502064707 0.0 50-51 0.0 2.7972321387986586E-4 0.0 0.20535180438955652 0.0 52-53 0.0 2.7972321387986586E-4 0.0 0.24363891928936318 0.0 54-55 0.0 3.496540173498323E-4 0.0 0.2821008611978447 0.0 56-57 0.0 3.496540173498323E-4 0.0 0.3301433231817117 0.0 58-59 0.0 3.496540173498323E-4 0.0 0.39010898715720793 0.0 60-61 0.0 3.496540173498323E-4 0.0 0.4547600149651919 0.0 62-63 6.993080346996647E-5 3.496540173498323E-4 0.0 0.5196208351835858 0.0 64-65 6.993080346996647E-5 3.496540173498323E-4 0.0 0.5868243373182236 0.0 66-67 6.993080346996647E-5 3.496540173498323E-4 0.0 0.6628391206900772 0.0 68-69 6.993080346996647E-5 3.496540173498323E-4 0.0 0.7575603939901467 0.0 70-71 6.993080346996647E-5 3.496540173498323E-4 0.0 0.8671419630275843 0.0 72-73 6.993080346996647E-5 3.496540173498323E-4 0.0 0.9794857988020853 0.0 74-75 6.993080346996647E-5 3.496540173498323E-4 0.0 1.0880184057874733 0.0 76-77 6.993080346996647E-5 4.195848208197988E-4 0.0 1.2085791109696955 0.0 78-79 6.993080346996647E-5 4.195848208197988E-4 0.0 1.3523918083056814 0.0 80-81 6.993080346996647E-5 4.195848208197988E-4 0.0 1.5222187645324952 0.0 82-83 6.993080346996647E-5 4.545502225547821E-4 0.0 1.7005772787826445 0.0 84-85 6.993080346996647E-5 4.895156242897653E-4 0.0 1.8768378689286949 0.0 86-87 6.993080346996647E-5 4.895156242897653E-4 0.0 2.0532383206816855 0.0 88-89 6.993080346996647E-5 4.895156242897653E-4 0.0 2.2678559565310126 0.0 90-91 6.993080346996647E-5 4.895156242897653E-4 0.0 2.5126137686758954 0.0 92-93 6.993080346996647E-5 4.895156242897653E-4 0.0 2.780868330786687 0.0 94-95 6.993080346996647E-5 4.895156242897653E-4 0.0 3.047059934195114 0.0 96-97 6.993080346996647E-5 4.895156242897653E-4 0.0 3.3113634059098525 0.0 98-99 6.993080346996647E-5 5.594464277597317E-4 0.0 3.6043385070472764 0.0 100-101 6.993080346996647E-5 5.94411829494715E-4 0.0 3.950810672839226 0.0 102-103 6.993080346996647E-5 6.293772312296982E-4 0.0 4.326618810686825 0.0 104-105 6.993080346996647E-5 6.293772312296982E-4 0.0 4.698091238719288 0.0 106-107 6.993080346996647E-5 6.293772312296982E-4 0.0 5.069388839743074 0.0 108-109 6.993080346996647E-5 6.293772312296982E-4 0.0 5.45365860481054 0.0 110-111 6.993080346996647E-5 6.293772312296982E-4 0.0 5.897928999255237 0.0 112-113 6.993080346996647E-5 6.293772312296982E-4 0.0 6.390136959478596 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3690 0.0 61.55592 1 GTATCAA 6300 0.0 49.775585 1 ATCAACG 8355 0.0 37.639862 3 TCAACGC 8410 0.0 37.322884 4 TATCAAC 8470 0.0 36.706894 2 CAACGCA 8510 0.0 36.70578 5 AACGCAG 8765 0.0 35.70578 6 GTACATG 7735 0.0 31.908857 1 TACATGG 7865 0.0 31.048862 2 ACATGGG 7960 0.0 30.379004 3 ACGCAGA 10265 0.0 30.314293 7 CGCAGAG 10315 0.0 30.051987 8 TACCTGG 1470 0.0 27.551937 2 GAGTACT 6445 0.0 27.00273 12-13 GCAGAGT 11075 0.0 26.968992 9 AGTACTT 6700 0.0 25.39779 12-13 CATGGGG 3155 0.0 25.296824 4 ATTCGCG 155 1.2732926E-11 24.949165 80-81 GTACTTT 6990 0.0 24.939924 14-15 CATGGGA 5275 0.0 24.275967 4 >>END_MODULE