FastQCFastQC Report
Thu 26 May 2016
SRR1364563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785761
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.2419696700734309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31640.17717936498781192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.11160508041109644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19120.10706919906975233No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75150.047.347891
GGTATCA43350.043.510951
GTACATG114400.040.1531561
TCAACGC89150.039.3773884
ATCAACG89100.039.2659263
CAACGCA89950.038.828735
AACGCAG91450.038.1875656
TACATGG117900.037.9002762
ACATGGG120000.036.0470243
TATCAAC97450.035.901432
TACCTGG15450.033.1196862
CATGGGA77900.032.6141934
ACGCAGA107800.032.2301067
CGCAGAG107600.031.4650488
GCAGAGT111700.028.8185779
GAGTACT45000.027.57292212-13
ATGGGTA9600.026.6509975
ATGGGAT30750.026.50895
ATGGGAG33500.026.4641365
CATGGGT18700.025.1363224
Produced by FastQC (version 0.11.5)