##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364563_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1785761 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.02567532833341 31.0 27.0 31.0 10.0 34.0 2 27.040535099601794 31.0 27.0 33.0 10.0 34.0 3 27.190459977566988 31.0 27.0 33.0 10.0 34.0 4 30.37515378597696 35.0 30.0 37.0 10.0 37.0 5 30.473183141529017 35.0 30.0 37.0 10.0 37.0 6 30.498253685683583 35.0 31.0 37.0 8.0 37.0 7 30.254310067248642 35.0 30.0 37.0 8.0 37.0 8 30.149554167662973 35.0 30.0 37.0 7.0 37.0 9 31.03097446970787 37.0 30.0 39.0 2.0 39.0 10-11 30.966144685655024 37.0 30.0 39.0 2.0 39.0 12-13 30.85791631690915 37.0 30.0 39.0 2.0 39.0 14-15 31.43904223465514 38.0 29.5 40.0 2.0 41.0 16-17 31.559750716921243 38.0 30.0 40.0 2.0 41.0 18-19 31.379911421517214 38.0 29.0 40.0 2.0 41.0 20-21 31.096734389428374 37.0 27.5 40.0 2.0 41.0 22-23 31.00034747091016 37.0 27.5 40.0 2.0 41.0 24-25 30.833701990355934 37.0 27.0 40.0 2.0 41.0 26-27 30.722971047077408 37.0 27.0 40.0 2.0 41.0 28-29 30.77877274730493 37.0 27.5 40.0 2.0 41.0 30-31 30.705065795478788 37.0 28.0 40.0 2.0 41.0 32-33 30.655126861881293 37.0 28.5 40.0 2.0 41.0 34-35 30.179029556586798 36.0 27.0 40.0 2.0 41.0 36-37 30.033001336685032 36.0 26.5 40.0 2.0 41.0 38-39 29.82109924004388 36.0 26.0 40.0 2.0 41.0 40-41 30.014590418314658 36.0 27.0 40.0 2.0 41.0 42-43 29.884821653065558 36.0 27.0 40.0 2.0 41.0 44-45 29.706982065349173 36.0 26.5 40.0 2.0 41.0 46-47 29.619074165019843 36.0 26.0 40.0 2.0 41.0 48-49 29.42672731681339 36.0 26.0 40.0 2.0 41.0 50-51 29.259686766594186 35.5 26.0 40.0 2.0 41.0 52-53 29.19565888156366 35.5 26.0 40.0 2.0 41.0 54-55 29.21001018613353 35.5 26.0 40.0 2.0 41.0 56-57 29.087265877124654 35.0 26.0 40.0 2.0 41.0 58-59 28.889573408759627 35.0 26.0 40.0 2.0 41.0 60-61 28.691875340541092 35.0 25.5 39.0 2.0 41.0 62-63 28.41377653560583 35.0 25.0 39.0 2.0 41.0 64-65 28.148862865747432 34.0 23.5 39.0 2.0 40.5 66-67 27.905337276376848 34.0 23.0 38.5 2.0 40.0 68-69 27.85756884599899 34.0 24.0 38.0 2.0 40.0 70-71 27.634270207491372 34.0 24.0 37.5 2.0 40.0 72-73 27.35962119230961 34.0 23.0 37.0 2.0 39.5 74-75 27.064399995296125 34.0 22.5 36.5 2.0 39.0 76-77 26.764387003635985 34.0 22.0 36.0 2.0 39.0 78-79 26.455204531849446 33.5 21.5 36.0 2.0 38.5 80-81 26.154461599284563 33.0 20.0 35.0 2.0 37.0 82-83 25.900202490702842 33.0 20.0 35.0 2.0 37.0 84-85 25.647297706691994 33.0 18.5 35.0 2.0 36.5 86-87 25.45149154898108 33.0 18.0 35.0 2.0 36.0 88-89 25.244027056252207 33.0 17.5 35.0 2.0 36.0 90-91 25.092528059465963 33.0 14.5 35.0 2.0 35.5 92-93 24.95636734143035 33.0 11.5 35.0 2.0 35.0 94-95 24.82889087621468 33.0 8.5 35.0 2.0 35.0 96-97 24.70678887040315 32.5 7.0 35.0 2.0 35.0 98-99 24.605309445104915 32.0 7.0 35.0 2.0 35.0 100-101 24.50407249346357 32.0 2.0 35.0 2.0 35.0 102-103 23.429176412745043 30.5 2.0 34.0 2.0 35.0 104-105 24.220331556126492 32.0 2.0 35.0 2.0 35.0 106-107 24.352365742112184 32.0 2.0 35.0 2.0 35.0 108-109 24.306329066431623 32.0 2.0 35.0 2.0 35.0 110-111 24.2112939525502 32.0 2.0 35.0 2.0 35.0 112-113 24.107335472104047 32.0 2.0 35.0 2.0 35.0 114-115 23.974589264744836 32.0 2.0 35.0 2.0 35.0 116-117 23.856990661124307 32.0 2.0 35.0 2.0 35.0 118-119 23.699642057363782 32.0 2.0 35.0 2.0 35.0 120-121 23.50661426697078 32.0 2.0 35.0 2.0 35.0 122-123 23.257561342195288 32.0 2.0 35.0 2.0 35.0 124-125 21.910229868386644 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8239481897593173 1101 2 0.93345623140819 1101 3 0.882369252533703 1101 4 0.8936514529401123 1101 5 0.838998041703352 1101 6 0.9849004097998808 1101 7 0.946832118370839 1101 8 1.0041182728088849 1101 9 1.2881235437623673 1101 10-11 1.2910434052842774 1101 12-13 1.0508487421224224 1101 14-15 1.3800674670105586 1101 16-17 1.1921702879210727 1101 18-19 1.470210297817328 1101 20-21 1.8595402047265672 1101 22-23 1.7194317505039294 1101 24-25 1.9983280148271625 1101 26-27 2.0793159566608495 1101 28-29 1.7722942282329655 1101 30-31 1.8807143545408849 1101 32-33 1.7756461903145428 1101 34-35 2.139762236493553 1101 36-37 2.032383675212447 1101 38-39 1.8804503945978261 1101 40-41 1.7400829406696907 1101 42-43 1.6539477756462553 1101 44-45 1.5365510262093842 1101 46-47 1.44093249076737 1101 48-49 1.406617484401977 1101 50-51 1.4323313800772297 1101 52-53 1.1553398704546431 1101 54-55 1.1430194885479388 1101 56-57 1.0502898051736835 1101 58-59 1.162420544039069 1101 60-61 1.2036998589339483 1101 62-63 1.058919986064634 1101 64-65 1.1231672629955973 1101 66-67 1.0491281098995877 1101 68-69 0.8369337877446696 1101 70-71 0.7912993117228204 1101 72-73 0.5953589114968167 1101 74-75 0.5525259170868111 1101 76-77 0.6891835360976586 1101 78-79 0.7916632392913172 1101 80-81 0.7934244298398738 1101 82-83 0.7586556180365278 1101 84-85 0.8942502246705821 1101 86-87 0.9278562604473812 1101 88-89 0.8836593317988246 1101 90-91 0.9089866616499194 1101 92-93 0.8506236187947991 1101 94-95 0.9595522135589754 1101 96-97 0.8523888714996417 1101 98-99 0.8456746313372179 1101 100-101 0.9065203281705365 1101 102-103 0.9413597047589448 1101 104-105 0.9283595917262808 1101 106-107 0.9148303423101254 1101 108-109 0.9475588406680089 1101 110-111 0.7563041343271983 1101 112-113 0.7268611559772218 1101 114-115 1.0141441139059957 1101 116-117 1.0260915581484618 1101 118-119 0.9065863328080042 1101 120-121 0.9427223010694483 1101 122-123 1.0408136927834555 1101 124-125 1.3377949912536273 1102 1 -0.37953840840869546 1102 2 -0.21163542180210015 1102 3 -0.06849455694557705 1102 4 0.030367761349477718 1102 5 0.15675362594986098 1102 6 -0.6539686072073039 1102 7 -1.2057270147627754 1102 8 -0.295976599142751 1102 9 0.015197869714775436 1102 10-11 0.09484446908728472 1102 12-13 0.018031606190838545 1102 14-15 0.257921519567752 1102 16-17 0.225084885182806 1102 18-19 0.41750428938027184 1102 20-21 0.75172704277173 1102 22-23 0.5623660917768234 1102 24-25 0.6411681314930533 1102 26-27 0.14056871237096757 1102 28-29 0.4986366231923114 1102 30-31 0.3276762583095092 1102 32-33 0.3782205360469213 1102 34-35 0.6620365739543033 1102 36-37 0.7025368441442161 1102 38-39 0.8032201664807737 1102 40-41 0.5500402518878147 1102 42-43 0.24207173494985668 1102 44-45 0.3430674849465589 1102 46-47 0.1359362849261636 1102 48-49 -0.18151539387206128 1102 50-51 0.2719332631308262 1102 52-53 0.013924271606914118 1102 54-55 -0.12986734482196027 1102 56-57 -0.11627750608040088 1102 58-59 0.10599949670738695 1102 60-61 0.014698747659000588 1102 62-63 -0.08486539761293344 1102 64-65 -0.22561144943593447 1102 66-67 -0.1821414408072748 1102 68-69 -0.27225404649194473 1102 70-71 -0.21164157492353652 1102 72-73 0.03481924185835794 1102 74-75 -0.32226274287384626 1102 76-77 -0.1799374777878846 1102 78-79 -0.18547679214086088 1102 80-81 -0.48339279240432376 1102 82-83 -0.3853842440071418 1102 84-85 -0.7627245180998372 1102 86-87 -0.3390960050789964 1102 88-89 -0.14323100442478065 1102 90-91 -0.52380537169552 1102 92-93 -0.0640071610037296 1102 94-95 -0.22011087952558483 1102 96-97 -0.14056967326963132 1102 98-99 0.0647076423169004 1102 100-101 0.05440390683549623 1102 102-103 -0.036353010887737014 1102 104-105 0.243350942482774 1102 106-107 0.07510014116576613 1102 108-109 -0.4342410651073827 1102 110-111 -0.0825077005711492 1102 112-113 -0.29554078030248476 1102 114-115 -0.2729979674508378 1102 116-117 -0.38862531848563364 1102 118-119 0.027505346398644548 1102 120-121 0.32615437843432105 1102 122-123 0.1819799200949248 1102 124-125 0.3541820170287373 1103 1 0.7423738425729773 1103 2 0.7178828199609804 1103 3 0.6550170552419239 1103 4 0.9426326500081288 1103 5 0.8105285176419734 1103 6 0.8683578980861881 1103 7 1.055816434569298 1103 8 1.1295158602292972 1103 9 1.5093084064867242 1103 10-11 1.476111992250388 1103 12-13 1.3126315963980026 1103 14-15 1.6192617820562951 1103 16-17 1.432176187146041 1103 18-19 1.4316474032642752 1103 20-21 1.9030214933342755 1103 22-23 1.8646861969599797 1103 24-25 1.9306916376293621 1103 26-27 1.8814114698595148 1103 28-29 1.8061952433065045 1103 30-31 1.9267153580872005 1103 32-33 1.7810379463171664 1103 34-35 2.183602903173778 1103 36-37 2.148697473296213 1103 38-39 2.3432145544329153 1103 40-41 2.0355477250089393 1103 42-43 1.9177650488481781 1103 44-45 1.5327536883194064 1103 46-47 1.6808965529601174 1103 48-49 1.6984398024583527 1103 50-51 1.6602437325118444 1103 52-53 1.7891394459816716 1103 54-55 1.7254362721819945 1103 56-57 1.5610686384832846 1103 58-59 1.4997484663591578 1103 60-61 1.4170949646114401 1103 62-63 1.4132822934074731 1103 64-65 1.6877269570495876 1103 66-67 1.5730895411736192 1103 68-69 1.604662111483286 1103 70-71 1.673170925601596 1103 72-73 1.5925293177296567 1103 74-75 1.5962949115740876 1103 76-77 1.5537386192631715 1103 78-79 1.453647887744978 1103 80-81 1.4777019103961635 1103 82-83 1.6051080140033562 1103 84-85 1.5514054420943175 1103 86-87 1.5439883352793906 1103 88-89 1.5472658602744502 1103 90-91 1.5940245997690852 1103 92-93 1.6133428224672386 1103 94-95 1.5552344555356896 1103 96-97 1.7422925174919008 1103 98-99 1.691710284354297 1103 100-101 1.6567853186436245 1103 102-103 1.4637041381282145 1103 104-105 1.4474950501367445 1103 106-107 1.5848221376409235 1103 108-109 1.691202256251458 1103 110-111 1.7128292874019166 1103 112-113 1.8771969371498791 1103 114-115 1.8987205563343075 1103 116-117 1.8854931597541373 1103 118-119 1.9067972809558498 1103 120-121 1.9205813570578378 1103 122-123 1.8151098203152074 1103 124-125 1.92640663917161 1104 1 0.10584687928730219 1104 2 0.25206036539143994 1104 3 0.10991825666231136 1104 4 0.05558830438075901 1104 5 0.06862727319096251 1104 6 0.07313075187976992 1104 7 0.25395946023909133 1104 8 0.21940561252717217 1104 9 0.47704301742359334 1104 10-11 0.3427928400781326 1104 12-13 0.28202863724434835 1104 14-15 0.3150236446381385 1104 16-17 0.321345338898805 1104 18-19 0.6167077665672274 1104 20-21 0.7132382485926065 1104 22-23 0.43323715834257115 1104 24-25 1.0925415132600413 1104 26-27 1.2094721987874344 1104 28-29 0.8603395387856096 1104 30-31 0.8893988099441827 1104 32-33 1.0205335354077434 1104 34-35 1.1704277520320971 1104 36-37 1.2565839715047602 1104 38-39 1.3025834305190713 1104 40-41 0.8419124953242019 1104 42-43 0.8807523537320456 1104 44-45 0.8299205954433582 1104 46-47 0.6836128948267728 1104 48-49 0.5736754898373597 1104 50-51 0.30454157965462514 1104 52-53 0.33992870607959347 1104 54-55 0.27781455303014724 1104 56-57 0.2241608541895701 1104 58-59 0.25759763076671405 1104 60-61 0.2651544459219437 1104 62-63 0.2307882737167688 1104 64-65 0.24969328599352636 1104 66-67 0.22545569767030926 1104 68-69 0.25329684996941637 1104 70-71 0.1394161086752419 1104 72-73 0.035691954808573456 1104 74-75 0.10381552770764912 1104 76-77 0.13242938145457117 1104 78-79 0.11034383147283222 1104 80-81 0.1903885421890834 1104 82-83 0.17201873736976836 1104 84-85 0.09403106241862602 1104 86-87 0.21147089231594052 1104 88-89 0.2328505747148526 1104 90-91 0.21544321384205034 1104 92-93 0.26767715633020117 1104 94-95 0.20859349628298318 1104 96-97 0.056160854071475086 1104 98-99 -0.005406230933889589 1104 100-101 0.21131898712867425 1104 102-103 0.24771010233485669 1104 104-105 0.23299673442025792 1104 106-107 0.2102400285829198 1104 108-109 0.2860160378502101 1104 110-111 0.3280892451344819 1104 112-113 0.2119764647494229 1104 114-115 0.2579918828114245 1104 116-117 0.36410646682525893 1104 118-119 0.3856584877131155 1104 120-121 0.3337103788310607 1104 122-123 0.3795854569992727 1104 124-125 0.628893322005883 1105 1 0.11562048744866615 1105 2 0.3742785043961909 1105 3 0.39738707397447115 1105 4 0.6019083336740572 1105 5 0.4132018791983363 1105 6 0.4481094627602289 1105 7 0.4977368507508224 1105 8 0.3578038793844165 1105 9 0.2727905397803134 1105 10-11 0.5358189545991081 1105 12-13 0.4516315592694653 1105 14-15 0.6702941557371354 1105 16-17 0.501689424003942 1105 18-19 0.5387293112223652 1105 20-21 1.079506053872631 1105 22-23 1.290562210081582 1105 24-25 1.1716491024821316 1105 26-27 1.0987221296763678 1105 28-29 0.8354175393406145 1105 30-31 0.856688598828903 1105 32-33 1.0190547107868149 1105 34-35 1.4235470794825105 1105 36-37 1.6008827949161635 1105 38-39 1.6814685542315182 1105 40-41 1.2804427514142773 1105 42-43 0.858437518785415 1105 44-45 1.0162568646791605 1105 46-47 1.0738639271257604 1105 48-49 0.9874443329412799 1105 50-51 0.9791482883971057 1105 52-53 1.0782834088146735 1105 54-55 0.8977637052831824 1105 56-57 0.8046782562169454 1105 58-59 0.7842132317775814 1105 60-61 0.8579562158004244 1105 62-63 0.8668730236670434 1105 64-65 0.8301777370293486 1105 66-67 0.7780238436953404 1105 68-69 0.5014125230357678 1105 70-71 0.6875583675014418 1105 72-73 0.7508872053135249 1105 74-75 0.7921368626012786 1105 76-77 0.8041205891110579 1105 78-79 0.7738522095601823 1105 80-81 0.8168298999446151 1105 82-83 0.6777964753887389 1105 84-85 0.7582582749883819 1105 86-87 0.5602599169096862 1105 88-89 0.7269874817177566 1105 90-91 0.5983722446845761 1105 92-93 0.541918334033042 1105 94-95 0.6778110505068824 1105 96-97 0.7530065046133743 1105 98-99 0.5219981427081244 1105 100-101 0.6097498053309103 1105 102-103 0.5266508324997154 1105 104-105 0.6649870788533967 1105 106-107 0.5812007696599863 1105 108-109 0.7093175101772644 1105 110-111 0.6176398637463372 1105 112-113 0.6570922213180843 1105 114-115 0.5631639180856638 1105 116-117 0.6808913446227329 1105 118-119 0.6824888934589239 1105 120-121 0.4996500504618595 1105 122-123 0.6319954117981865 1105 124-125 0.7439846511708055 1106 1 0.1877441082660276 1106 2 0.34444123224470147 1106 3 0.07647788977109826 1106 4 0.14154361465656606 1106 5 0.03770400961545306 1106 6 0.14093759871149203 1106 7 -0.025693702520097617 1106 8 -0.011269988032474032 1106 9 0.2937255076865313 1106 10-11 0.3913750735613739 1106 12-13 0.4579082163834727 1106 14-15 0.6433795745757003 1106 16-17 0.6474780387977006 1106 18-19 0.6325838053271546 1106 20-21 1.0282490748214492 1106 22-23 0.9706848447429195 1106 24-25 1.1095866402851122 1106 26-27 1.2975671354592109 1106 28-29 1.1677063211164729 1106 30-31 0.836597283901753 1106 32-33 0.77013081386729 1106 34-35 1.0873862365208637 1106 36-37 1.0731318237905434 1106 38-39 1.0858763838153642 1106 40-41 0.9909409283302892 1106 42-43 0.9184729273279828 1106 44-45 0.9142766709207528 1106 46-47 0.5219468207216522 1106 48-49 0.6503251082654273 1106 50-51 0.7039955599715881 1106 52-53 0.5504360009914073 1106 54-55 0.5144521766862056 1106 56-57 0.5115235401463529 1106 58-59 0.3589664002342019 1106 60-61 0.4451938343439217 1106 62-63 0.37332594339230596 1106 64-65 0.5265528470001364 1106 66-67 0.3476689606830057 1106 68-69 0.34063519752263716 1106 70-71 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78-79 -2.9730508617865326 2216 80-81 -3.0922375523078998 2216 82-83 -2.787161398178185 2216 84-85 -2.7724687253837494 2216 86-87 -2.8198740129658795 2216 88-89 -2.868004968898255 2216 90-91 -2.8362871602334323 2216 92-93 -2.830790514670099 2216 94-95 -2.750042342995769 2216 96-97 -2.7010372239846347 2216 98-99 -2.7841153976900515 2216 100-101 -2.8652836908500454 2216 102-103 -2.6411906994737677 2216 104-105 -2.842618345733424 2216 106-107 -2.8774967729120924 2216 108-109 -3.0030657450350766 2216 110-111 -2.9841109041589 2216 112-113 -2.959432040624254 2216 114-115 -3.088013226602932 2216 116-117 -3.128624873586002 2216 118-119 -3.277792153718078 2216 120-121 -3.3562844759956825 2216 122-123 -3.3235973623986226 2216 124-125 -3.3161256271876134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 167941.0 3 26594.0 4 16143.0 5 12639.0 6 11461.0 7 11915.0 8 12480.0 9 13003.0 10 13827.0 11 14698.0 12 16016.0 13 18501.0 14 21162.0 15 18192.0 16 13548.0 17 11768.0 18 10639.0 19 10173.0 20 9629.0 21 10454.0 22 12202.0 23 16243.0 24 22027.0 25 27616.0 26 33446.0 27 36099.0 28 40715.0 29 47024.0 30 52510.0 31 58968.0 32 70992.0 33 87415.0 34 109839.0 35 146112.0 36 215677.0 37 277306.0 38 90482.0 39 305.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.06951382883872 19.2994963999767 13.102449449579511 22.528540321605067 2 14.91609615411539 20.660366528063616 41.070463550198106 23.353073767622888 3 19.26335868030004 27.08944466285622 28.402715474418383 25.244481182425353 4 11.579800382836643 16.960906532080212 37.91774052456908 33.54155256051406 5 12.605557839367263 39.932254801698555 33.59267975496132 13.869507603972862 6 30.27220541319683 37.80294745000272 18.14142764904547 13.783419487754983 7 28.302451055612742 33.63584636631538 20.6779465478792 17.383756030192686 8 25.927881726614032 36.60433843050666 19.41138819808474 18.056391644794573 9 27.083228333316534 16.992866718858675 19.582643133222902 36.341261814601886 10-11 24.34505513335771 28.39797710891883 26.919531785048502 20.337435972674957 12-13 25.194805922088833 26.092555388330823 27.312365685485286 21.400273004095062 14-15 22.487548785460476 26.87014665884008 25.52851849030564 25.1137860653938 16-17 21.433991054676042 30.51907694384623 26.054425369787953 21.992506631689775 18-19 21.391551878192658 28.829083362011197 28.676812984927807 21.10255177486833 20-21 22.37206645403913 27.857800901100195 28.895046189085434 20.875086455775246 22-23 22.069542505913557 27.894169772776145 28.545948767113465 21.490338954196833 24-25 21.701622537085484 27.880155941881913 28.946053764217712 21.472167756814898 26-27 21.530394318754016 28.192326240364114 28.672587338403023 21.60469210247885 28-29 21.352953067977488 27.92553057449714 29.02274278693638 21.69877357058899 30-31 21.52062090204844 27.940312181414324 28.8045272381042 21.73453967843304 32-33 21.473507059370398 28.0359773069664 28.636065794116167 21.854449839547037 34-35 21.698966487862084 27.698338114274385 29.066198765430716 21.536496632432815 36-37 21.77247971244159 27.52406797924745 28.736462943054818 21.96698936525614 38-39 21.486105923469044 27.90541399436991 28.91291163823154 21.695568443929506 40-41 21.687947235019813 27.77045553206089 28.82908795563709 21.71250927728221 42-43 21.535378686624558 27.899546332676117 28.462507130118038 22.102567850581288 44-45 21.66910868552174 27.9910810302183 28.406140221170734 21.933670063089224 46-47 21.879804745503016 28.322962339895486 27.836114129742374 21.961118784859124 48-49 21.83856860183252 28.31164866496102 27.68784160179471 22.16194113141175 50-51 21.822486771153415 28.26476285517962 27.694644228168414 22.218106145498552 52-53 22.015824066042434 28.46850726384998 27.454093800906172 22.061574869201422 54-55 21.98743549510439 28.29710436133887 27.51345406825764 22.202006075299092 56-57 21.81325215347004 28.630210210260664 27.350882542605287 22.205655093664014 58-59 22.009376999332215 28.142393752773692 27.505071391472224 22.343157856421872 60-61 22.03043854567224 28.335629865313372 27.65868261293351 21.97524897608088 62-63 21.780638909272195 28.306128622082312 27.912005735955592 22.001226732689904 64-65 21.72689242481185 28.13784535972188 27.75269810313646 22.38256411232981 66-67 21.852621121741098 28.56964458113348 27.27274763625286 22.304986660872565 68-69 21.744626912738408 28.273268908377318 27.70049060875478 22.281613570129494 70-71 21.75463895010066 28.189024441463967 27.792400157921705 22.263936450513665 72-73 21.88712531923473 28.376299341368338 27.46958869124961 22.26698664814732 74-75 21.726619631630438 28.64235471600063 27.323729211243837 22.3072964411251 76-77 21.88317637676744 28.325153223060518 27.590595374634862 22.201075025537175 78-79 21.803786445716 28.32699981744242 27.55219410686226 22.317019629979313 80-81 21.757872460291196 28.645529703693875 27.35893804457332 22.237659791441615 82-83 21.946797593697806 28.600360357333354 27.320489828375273 22.132352220593567 84-85 21.829320944964078 28.523525824564427 27.263558785302177 22.383594445169315 86-87 21.77693201791291 28.383556703354735 27.565166429170052 22.2743448495623 88-89 22.01974885461124 28.257776881630093 27.39641092852102 22.32606333523765 90-91 21.885191656708102 28.469717800421158 27.483925101151645 22.161165441719092 92-93 21.95665577806289 28.448348760877636 27.430237240144955 22.164758220914518 94-95 21.815832803302797 28.10819848712341 27.542459156841147 22.533509552732646 96-97 22.100595419629272 28.117663499552897 27.36527444165232 22.41646663916551 98-99 21.714478671074914 28.23187422161295 27.35610479441622 22.697542312895912 100-101 21.689838026830035 28.14731513432519 27.58116649916679 22.58168033967798 102-103 21.945573903786677 27.981572002076426 27.525099943385484 22.547754150751416 104-105 21.74545228511333 28.075713488922187 27.751504705073327 22.427329520891153 106-107 21.627682331332316 28.129507884598155 27.70848266284383 22.534327121225697 108-109 21.71449594878598 28.278028246781062 27.135070146564967 22.87240565786799 110-111 21.536669569283408 28.36536011580601 27.43726007912664 22.660710235783945 112-113 21.475326205466466 28.339962626577687 27.680131887749816 22.50457928020603 114-115 21.517353106042748 28.471223192801276 27.35046851174373 22.660955189412245 116-117 21.341517705896816 28.413656698740763 27.44997790857791 22.794847686784514 118-119 21.46617044498116 28.266604545625086 27.412206896667584 22.85501811272617 120-121 21.285021143652802 28.397873063045125 27.576878310389326 22.740227482912744 122-123 21.416387747296532 28.477719022870364 27.43564228359786 22.670250946235246 124-125 21.45565080870267 28.365379633625608 27.336119140150767 22.842850417520953 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1787.0 1 1914.5 2 2102.0 3 1801.5 4 1216.0 5 882.0 6 803.5 7 1006.5 8 1389.5 9 1567.0 10 1440.5 11 1329.5 12 1407.5 13 1578.0 14 1765.0 15 1942.0 16 2099.0 17 2288.0 18 2488.0 19 2783.0 20 3244.0 21 3859.5 22 4586.0 23 5538.0 24 6713.5 25 8313.5 26 10845.0 27 13848.0 28 16206.0 29 19127.5 30 23408.5 31 27251.0 32 31708.5 33 37469.5 34 43392.0 35 47930.5 36 53229.5 37 58356.0 38 59186.0 39 59410.5 40 58730.0 41 57530.0 42 56842.0 43 57743.5 44 60295.0 45 61538.5 46 60448.5 47 58788.0 48 59722.5 49 60231.0 50 59405.5 51 57709.0 52 55869.0 53 54996.0 54 53292.0 55 49500.5 56 44789.5 57 40849.5 58 38688.0 59 35764.0 60 30899.0 61 25602.5 62 20120.5 63 15031.5 64 11116.5 65 8509.0 66 6373.0 67 4826.0 68 3664.5 69 2690.0 70 1936.5 71 1403.0 72 1090.5 73 903.5 74 710.5 75 537.0 76 369.5 77 238.0 78 168.5 79 126.5 80 91.5 81 57.5 82 46.5 83 38.0 84 28.5 85 23.0 86 19.0 87 15.0 88 14.0 89 12.0 90 7.5 91 7.5 92 7.5 93 5.0 94 5.0 95 4.5 96 2.5 97 2.5 98 2.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01215168211199595 2 0.0048158740167357226 3 0.008007790516200096 4 0.008007790516200096 5 0.01383163816434562 6 7.83979491096513E-4 7 0.009127761217766543 8 0.0 9 0.0026319311486811504 10-11 0.0 12-13 0.004115892328256693 14-15 0.022007424285780685 16-17 0.03261914668312277 18-19 0.006803822012016165 20-21 0.009295756823001511 22-23 0.0018479516575846376 24-25 0.012543671857544209 26-27 0.02116744625960585 28-29 4.4798828062657875E-4 30-31 0.0020439465303587656 32-33 0.010695720199959569 34-35 0.011143708480586148 36-37 0.013747640361728137 38-39 0.0 40-41 0.02721528804806466 42-43 0.011283704818281955 44-45 0.019767482882647788 46-47 0.005123865959666496 48-49 0.028755247762718525 50-51 0.004479882806265788 52-53 0.0 54-55 0.018731509983698828 56-57 0.005403858635058107 58-59 4.7598754816573994E-4 60-61 0.005459857170136429 62-63 0.02973522212658917 64-65 0.0190395019266296 66-67 0.002071945797897927 68-69 0.023967373013521965 70-71 0.003695903315169275 72-73 0.013495646953875687 74-75 0.0 76-77 0.007223811025103583 78-79 0.0012879663068014141 80-81 0.004143891595795854 82-83 0.0018759509251237989 84-85 0.0 86-87 0.006803822012016165 88-89 0.01909550046170792 90-91 0.025003345912470932 92-93 0.00571185057798888 94-95 0.027243287315603822 96-97 0.021363441132379975 98-99 9.519750963314799E-4 100-101 0.010499725327185442 102-103 0.0 104-105 0.0016239575172713482 106-107 5.599853507832234E-5 108-109 0.0 110-111 8.959765612531575E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.799926753916117E-5 122-123 0.0 124-125 0.022483411833946424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1785761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.497631395805215 #Duplication Level Percentage of deduplicated Percentage of total 1 81.38310331250048 47.60718779421388 2 10.038849289460801 11.74497810745838 3 3.4601450951073955 6.072308770487874 4 1.609776963015409 3.7667255764773664 5 0.8843542834859053 2.5866315449329957 6 0.5569287903244068 1.9547409054065306 7 0.3596166073800399 1.4725703819629308 8 0.2595975440450376 1.2148673154242335 9 0.19212187695191515 1.0114807266903039 >10 1.1019032091916456 11.958594477925766 >50 0.09814918083386182 3.9641020397110758 >100 0.05388366700089756 5.550479024966413 >500 0.0011776355265775498 0.44146296336956914 >1k 3.925451755258499E-4 0.6538703709726128 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4321 0.2419696700734309 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3164 0.17717936498781192 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1993 0.11160508041109644 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1912 0.10706919906975233 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.599853507832235E-5 0.0 8 0.0 0.0 0.0 5.599853507832235E-5 0.0 9 0.0 0.0 0.0 5.599853507832235E-5 0.0 10-11 5.599853507832235E-5 0.0 0.0 8.399780261748353E-5 0.0 12-13 2.7999267539161174E-4 0.0 0.0 1.6799560523496706E-4 0.0 14-15 3.9198974554825646E-4 0.0 0.0 2.239941403132894E-4 0.0 16-17 4.479882806265788E-4 0.0 0.0 2.239941403132894E-4 0.0 18-19 5.879846183223848E-4 0.0 0.0 3.639904780090953E-4 0.0 20-21 6.719824209398683E-4 0.0 0.0 5.039868157049011E-4 0.0 22-23 6.719824209398683E-4 0.0 0.0 9.79974363870641E-4 0.0 24-25 7.839794910965129E-4 0.0 0.0 0.0016239575172713482 0.0 26-27 7.839794910965129E-4 0.0 0.0 0.0021559436005154104 0.0 28-29 8.679772937139965E-4 0.0 0.0 0.0031359179643860517 0.0 30-31 8.959765612531576E-4 0.0 0.0 0.005599853507832235 0.0 32-33 9.239758287923188E-4 0.0 0.0 0.01072371946749873 0.0 34-35 0.0010639721664881248 0.0 0.0 0.01621157590517432 0.0 36-37 0.0012319677717230917 0.0 0.0 0.027243287315603822 0.0 38-39 0.0012319677717230917 0.0 0.0 0.04899871819353206 0.0 40-41 0.0012319677717230917 0.0 0.0 0.0745060509217079 0.0 42-43 0.0013999633769580587 0.0 0.0 0.0964294774048711 0.0 44-45 0.0018199523900454765 0.0 0.0 0.12336477277754414 0.0 46-47 0.002071945797897927 0.0 0.0 0.15895184181981797 0.0 48-49 0.0021839428680545717 0.0 0.0 0.19770282809401707 0.0 50-51 0.0021839428680545717 0.0 0.0 0.24633755580954003 0.0 52-53 0.0021839428680545717 0.0 0.0 0.290632397056493 0.0 54-55 0.0021839428680545717 0.0 0.0 0.3371111811715005 0.0 56-57 0.0023519384732895387 0.0 0.0 0.3933897089252145 0.0 58-59 0.0023519384732895387 0.0 0.0 0.4609799407647496 0.0 60-61 0.0023519384732895387 0.0 0.0 0.5405258598435065 0.0 62-63 0.0023519384732895387 0.0 0.0 0.6204357694002725 0.0 64-65 0.0024079370083678613 0.0 0.0 0.6975457522031223 0.0 66-67 0.0025759326136028282 0.0 0.0 0.785911440556715 0.0 68-69 0.0026319311486811504 0.0 0.0 0.8950525854243653 0.0 70-71 0.002715928951298634 0.0 0.0 1.0252771787490038 0.0 72-73 0.0027999267539161174 0.0 0.0 1.1558657625516515 0.0 74-75 0.00285592528899444 0.0 0.0 1.2838224152056181 0.0 76-77 0.002883924556533601 0.0 0.0 1.4259746965019395 0.0 78-79 0.002911923824072762 0.0 0.0 1.596994222631136 0.0 80-81 0.0033879113722385026 0.0 0.0 1.7920371203089327 0.0 82-83 0.0034719091748559856 0.0 0.0 1.9954517989809386 0.0 84-85 0.0035559069774734695 0.0 0.0 2.1968785296576643 0.0 86-87 0.003667904047630114 0.0 0.0 2.4024211526626464 0.0 88-89 0.003695903315169275 0.0 0.0 2.6423468762057185 0.0 90-91 0.0040318945256392095 0.0 0.0 2.929927353100443 0.0 92-93 0.004115892328256692 0.0 0.0 3.2308914798788866 0.0 94-95 0.004143891595795854 0.0 0.0 3.5307356359557636 0.0 96-97 0.004171890863335016 0.0 0.0 3.8323717451551467 0.0 98-99 0.0045078820738049495 0.0 0.0 4.158927202464383 0.0 100-101 0.004815874016735722 0.0 0.0 4.550384961929396 0.0 102-103 0.005179864494744817 0.0 0.0 4.983225638817288 0.0 104-105 0.00643983153400707 0.0 0.0 5.40475461161936 0.0 106-107 0.0067198242093986825 0.0 0.0 5.818107798299996 0.0 108-109 0.0067198242093986825 0.0 0.0 6.241092733014105 0.0 110-111 0.006775822744477004 0.0 0.0 6.737463747948354 0.0 112-113 0.006803822012016165 0.0 0.0 7.276477647344746 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7515 0.0 47.34789 1 GGTATCA 4335 0.0 43.51095 1 GTACATG 11440 0.0 40.153156 1 TCAACGC 8915 0.0 39.377388 4 ATCAACG 8910 0.0 39.265926 3 CAACGCA 8995 0.0 38.82873 5 AACGCAG 9145 0.0 38.187565 6 TACATGG 11790 0.0 37.900276 2 ACATGGG 12000 0.0 36.047024 3 TATCAAC 9745 0.0 35.90143 2 TACCTGG 1545 0.0 33.119686 2 CATGGGA 7790 0.0 32.614193 4 ACGCAGA 10780 0.0 32.230106 7 CGCAGAG 10760 0.0 31.465048 8 GCAGAGT 11170 0.0 28.818577 9 GAGTACT 4500 0.0 27.572922 12-13 ATGGGTA 960 0.0 26.650997 5 ATGGGAT 3075 0.0 26.5089 5 ATGGGAG 3350 0.0 26.464136 5 CATGGGT 1870 0.0 25.136322 4 >>END_MODULE