FastQCFastQC Report
Thu 26 May 2016
SRR1364563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785761
Sequences flagged as poor quality0
Sequence length125
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83300.46646779720242515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73390.41097324893980774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49470.27702475303246066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22170.12414875226864067No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.060.3486671
GTATCAA99050.047.4935951
ATCAACG124950.037.9012573
TCAACGC126300.037.2131464
CAACGCA127600.036.791725
AACGCAG131050.036.0955736
TATCAAC132050.035.4573972
GTACATG111200.031.3127041
ACGCAGA155750.030.2184547
CGCAGAG156200.030.055218
TACATGG113600.029.9944172
TATAACG1454.0318337E-628.7576082
ACATGGG114850.028.7343563
GCAGAGT162550.028.1474389
GAGTACT95450.027.73931512-13
GTACTTT101250.026.23844314-15
TACCTGG16050.025.9803962
CATGGGA72950.025.9671654
AGTACTT101450.024.57387412-13
GAGTACA87400.024.55861
Produced by FastQC (version 0.11.5)