##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364563_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1785761 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.911969742871527 31.0 30.0 34.0 26.0 34.0 2 29.76917627834856 31.0 30.0 34.0 26.0 34.0 3 30.075362268523055 31.0 30.0 34.0 26.0 34.0 4 33.6172735321244 35.0 35.0 37.0 28.0 37.0 5 33.01188848899713 35.0 33.0 37.0 26.0 37.0 6 33.035286356908905 35.0 33.0 37.0 26.0 37.0 7 32.813918547890786 35.0 33.0 37.0 25.0 37.0 8 32.74030679357428 35.0 33.0 37.0 25.0 37.0 9 33.91258908666949 38.0 33.0 39.0 23.0 39.0 10-11 33.86178077581491 38.0 33.0 39.0 23.0 39.0 12-13 33.73681724486087 38.0 33.0 39.0 22.5 39.0 14-15 34.66930625094848 39.0 33.0 41.0 22.5 41.0 16-17 34.688467549688895 39.0 33.0 41.0 22.5 41.0 18-19 34.756190778049245 39.0 33.5 41.0 23.0 41.0 20-21 34.784640553803115 39.0 34.0 41.0 23.0 41.0 22-23 34.74619867944254 39.0 34.0 41.0 23.0 41.0 24-25 34.71314414414919 39.0 34.0 41.0 23.5 41.0 26-27 34.58806161630812 39.0 33.5 41.0 23.0 41.0 28-29 34.43890671820026 39.0 33.0 41.0 22.5 41.0 30-31 34.25721471126315 38.0 33.0 40.5 22.0 41.0 32-33 34.00850561749304 38.0 33.0 40.0 20.0 41.0 34-35 33.806963529834064 38.0 33.0 40.0 18.0 41.0 36-37 33.586630013758835 38.0 33.0 40.0 16.5 41.0 38-39 33.40372451856659 38.0 32.0 40.0 15.5 41.0 40-41 33.414565834957756 38.0 33.0 40.0 15.0 41.0 42-43 33.24907280425544 38.0 32.0 40.0 11.5 41.0 44-45 33.0505350380034 38.0 32.0 40.0 8.5 41.0 46-47 32.72742517055754 38.0 31.5 40.0 4.5 41.0 48-49 32.38308233856603 38.0 31.0 40.0 2.0 41.0 50-51 32.19855204587848 38.0 31.0 40.0 2.0 41.0 52-53 32.01881606777167 37.5 30.5 40.0 2.0 41.0 54-55 31.837305776080896 37.0 30.0 40.0 2.0 41.0 56-57 31.623875479417457 37.0 30.0 40.0 2.0 41.0 58-59 31.422454628586916 37.0 30.0 40.0 2.0 41.0 60-61 31.198771560135988 36.0 30.0 40.0 2.0 41.0 62-63 31.025836324121762 36.0 29.5 40.0 2.0 41.0 64-65 30.88391559676799 36.0 29.5 39.0 2.0 41.0 66-67 30.587463831946156 35.0 29.0 39.0 2.0 41.0 68-69 30.298587268957043 35.0 29.0 39.0 2.0 41.0 70-71 29.989508674453077 35.0 29.0 38.5 2.0 40.0 72-73 29.70122485595777 35.0 29.0 37.5 2.0 40.0 74-75 29.351431966539757 35.0 29.0 37.0 2.0 39.0 76-77 29.039627083355498 35.0 28.5 36.5 2.0 39.0 78-79 28.749223160322124 34.0 28.0 36.0 2.0 39.0 80-81 28.46467192418246 34.0 28.0 36.0 2.0 37.5 82-83 28.176275268638975 34.0 28.0 35.0 2.0 37.0 84-85 27.93651362080368 34.0 27.0 35.0 2.0 36.5 86-87 27.721582843392817 34.0 27.0 35.0 2.0 36.0 88-89 27.53729810428159 34.0 26.5 35.0 2.0 36.0 90-91 27.383833278921422 34.0 26.0 35.0 2.0 36.0 92-93 27.263688701903558 34.0 26.0 35.0 2.0 35.0 94-95 27.14618585577801 34.0 26.0 35.0 2.0 35.0 96-97 27.026949015013766 34.0 26.0 35.0 2.0 35.0 98-99 26.941119780306547 34.0 25.5 35.0 2.0 35.0 100-101 26.838214632305217 34.0 25.0 35.0 2.0 35.0 102-103 25.577812204432732 31.5 23.5 34.0 2.0 35.0 104-105 26.500202434704306 33.0 25.0 35.0 2.0 35.0 106-107 26.656491266188475 34.0 25.0 35.0 2.0 35.0 108-109 26.658492933824853 34.0 25.0 35.0 2.0 35.0 110-111 26.57114977872179 34.0 25.0 35.0 2.0 35.0 112-113 26.485363942879253 34.0 25.0 35.0 2.0 35.0 114-115 26.384986008766013 34.0 25.0 35.0 2.0 35.0 116-117 26.25618209827631 34.0 24.5 35.0 2.0 35.0 118-119 26.123580927122948 34.0 24.0 35.0 2.0 35.0 120-121 25.92231463224922 34.0 23.5 35.0 2.0 35.0 122-123 25.631278205762136 33.5 21.0 35.0 2.0 35.0 124-125 23.880083897005257 31.0 9.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6361165114422569 1101 2 0.8303396473244256 1101 3 0.5825271362927431 1101 4 0.5099057206610098 1101 5 0.6582023100021388 1101 6 0.627031804504206 1101 7 0.8229977671622635 1101 8 0.8255484155062689 1101 9 0.9894769538235053 1101 10-11 1.0540266637202436 1101 12-13 0.8694744251363176 1101 14-15 1.0874843109302148 1101 16-17 1.0618552795650302 1101 18-19 1.0194315812972974 1101 20-21 1.0421054767079596 1101 22-23 1.1431394369051517 1101 24-25 1.045767126516644 1101 26-27 1.1155630184794632 1101 28-29 1.1393728805066345 1101 30-31 1.128221282106665 1101 32-33 1.1449092732694837 1101 34-35 1.3077425726550516 1101 36-37 1.4157576483705796 1101 38-39 1.397705191308468 1101 40-41 1.065579686937049 1101 42-43 1.0387185507455214 1101 44-45 1.025160245662299 1101 46-47 1.0944719234709268 1101 48-49 1.3158416828660577 1101 50-51 1.2485978137181277 1101 52-53 1.2700517020641087 1101 54-55 1.2553695811008119 1101 56-57 1.221385882009141 1101 58-59 1.3480829500753764 1101 60-61 1.2407892515217043 1101 62-63 1.2710328253902787 1101 64-65 1.27124297906823 1101 66-67 1.3579488460214968 1101 68-69 1.3494335607616748 1101 70-71 1.289909791573269 1101 72-73 1.182261886629977 1101 74-75 1.2447641751958827 1101 76-77 1.2150943898087654 1101 78-79 1.2489472734275182 1101 80-81 1.0281923737837282 1101 82-83 1.1645495645559691 1101 84-85 1.1488818084592403 1101 86-87 1.2075877319904968 1101 88-89 1.228682410950455 1101 90-91 1.2043166274910355 1101 92-93 1.2063371590275125 1101 94-95 1.1698580175722597 1101 96-97 1.2535530704885431 1101 98-99 1.2259324543005619 1101 100-101 1.2918813756824754 1101 102-103 1.2353836949442716 1101 104-105 1.2362879674190346 1101 106-107 1.171544881927442 1101 108-109 1.2111506004729797 1101 110-111 1.1456711692844728 1101 112-113 1.131214477920068 1101 114-115 1.2797206610660048 1101 116-117 1.2884069714008497 1101 118-119 1.3572074691067897 1101 120-121 1.3759281841613245 1101 122-123 1.346119106637687 1101 124-125 1.654263008620262 1102 1 0.07076210824005713 1102 2 -0.6271964870930908 1102 3 -0.08063370367287703 1102 4 0.07174978972548729 1102 5 0.20101876262550178 1102 6 0.033597892996219514 1102 7 0.18951206808400656 1102 8 0.3132149898663883 1102 9 0.6396832769419802 1102 10-11 0.7041375394696345 1102 12-13 0.6236668806943086 1102 14-15 0.7179453281963362 1102 16-17 0.6790089771615797 1102 18-19 0.6643553564673184 1102 20-21 0.4837103159686933 1102 22-23 0.6247931147217329 1102 24-25 0.6357850004211016 1102 26-27 0.7639129729855227 1102 28-29 0.39251002046613337 1102 30-31 0.653232880364591 1102 32-33 0.8150060512914123 1102 34-35 0.8825066335198315 1102 36-37 0.5160324102017029 1102 38-39 -0.026429542686834395 1102 40-41 -0.41602054898410756 1102 42-43 0.07803121460462847 1102 44-45 -0.40465162879600314 1102 46-47 -0.07541079926058813 1102 48-49 -0.10481299833470104 1102 50-51 -0.0937921107223687 1102 52-53 -0.42008643408684776 1102 54-55 -0.3141451621731015 1102 56-57 -0.24364549622353238 1102 58-59 -0.29038226773811004 1102 60-61 -0.33710292244137463 1102 62-63 -0.37353282434592217 1102 64-65 -0.4290115712250575 1102 66-67 -0.33247304285820434 1102 68-69 -0.1347481416827705 1102 70-71 -0.7208374958729173 1102 72-73 -0.558434224626982 1102 74-75 -0.3461478254946222 1102 76-77 -0.7527540164647206 1102 78-79 -0.6051390895386106 1102 80-81 -0.6404141449766811 1102 82-83 -0.8675518959254376 1102 84-85 -0.7338852301908325 1102 86-87 -0.3793377046973987 1102 88-89 -0.6449265713158994 1102 90-91 -0.6299589039609224 1102 92-93 -0.4912739954397054 1102 94-95 -0.2103095727592894 1102 96-97 -0.4748893823721829 1102 98-99 -0.6442514275374656 1102 100-101 -0.46217880578980086 1102 102-103 -0.4776991399936392 1102 104-105 -0.44662932711198167 1102 106-107 -0.3661663951756182 1102 108-109 -0.3441829798103413 1102 110-111 -0.3703200412140184 1102 112-113 -0.20615485736488637 1102 114-115 -0.13731586715282518 1102 116-117 -0.3970338163456333 1102 118-119 -0.09167747977096496 1102 120-121 0.17315812456965674 1102 122-123 0.1405550357031018 1102 124-125 0.2566819197146728 1103 1 0.24172864079123713 1103 2 -0.12777666911814123 1103 3 0.23356072119072735 1103 4 0.17902971965745706 1103 5 0.28313704558802755 1103 6 0.4219359590503373 1103 7 0.39522462287535376 1103 8 0.4689859684806308 1103 9 0.721056522162975 1103 10-11 0.7705861272298122 1103 12-13 0.6913941143081885 1103 14-15 0.8164578457864948 1103 16-17 0.727802647629197 1103 18-19 0.6965800858098632 1103 20-21 0.7390521192352679 1103 22-23 0.7792923285424891 1103 24-25 0.7444105760036805 1103 26-27 0.8221502829342597 1103 28-29 0.9530942065439092 1103 30-31 0.8774201402336956 1103 32-33 0.8598975087231437 1103 34-35 0.7924365249323557 1103 36-37 0.8904089499294585 1103 38-39 0.8537089071421278 1103 40-41 0.6701564005940881 1103 42-43 0.6782773972097118 1103 44-45 0.7720890549003485 1103 46-47 0.8536309109740614 1103 48-49 0.6845960649052856 1103 50-51 0.6762929797440194 1103 52-53 0.7570743564441216 1103 54-55 0.7950869607600133 1103 56-57 0.7447320309001562 1103 58-59 0.7929930038464725 1103 60-61 0.9252901567476073 1103 62-63 0.7815321574895258 1103 64-65 0.7369393927462937 1103 66-67 0.6858927966036177 1103 68-69 0.8219344253032688 1103 70-71 0.600480999287015 1103 72-73 0.5778529957520782 1103 74-75 0.6565100745564614 1103 76-77 0.7018168617874494 1103 78-79 0.6784298368102881 1103 80-81 0.5993429260732377 1103 82-83 0.6939894094131347 1103 84-85 0.7881682056601598 1103 86-87 0.8350350627607206 1103 88-89 0.895986329527588 1103 90-91 0.7707696337225123 1103 92-93 0.7675336167802449 1103 94-95 0.8530166796009127 1103 96-97 0.8902578447923304 1103 98-99 0.8346044506118524 1103 100-101 0.7289942136165273 1103 102-103 0.5609120824216411 1103 104-105 0.5560757987418903 1103 106-107 0.6220362805862152 1103 108-109 0.739202432284042 1103 110-111 0.7384076637887027 1103 112-113 0.7325506862779818 1103 114-115 0.7947312728902212 1103 116-117 0.8360063258764647 1103 118-119 0.763623384808902 1103 120-121 0.7745590215122355 1103 122-123 0.922949256423756 1103 124-125 1.3262491488319554 1104 1 0.43054850026242875 1104 2 0.6843930404089207 1104 3 0.32584110872613437 1104 4 0.15368576560874914 1104 5 0.3091988532584651 1104 6 0.24251747867000262 1104 7 0.5817197462068009 1104 8 0.34165294421001136 1104 9 0.43489070487748904 1104 10-11 0.5585351443319055 1104 12-13 0.43471584290512055 1104 14-15 0.6104542214714286 1104 16-17 0.5451017967866036 1104 18-19 0.4312398042488681 1104 20-21 0.29780272105506356 1104 22-23 0.4821500490074513 1104 24-25 0.6289798489458391 1104 26-27 0.5797047281734606 1104 28-29 0.6444013171783496 1104 30-31 0.5739806403415457 1104 32-33 0.6766973402812653 1104 34-35 0.7619816019449885 1104 36-37 0.8508414130970081 1104 38-39 0.8570477045526061 1104 40-41 0.8097565351527436 1104 42-43 0.7390331177791367 1104 44-45 0.7013992144844252 1104 46-47 0.8882681939502035 1104 48-49 0.7544973488686892 1104 50-51 0.6667777995417836 1104 52-53 0.6528727273967689 1104 54-55 0.2737578449689373 1104 56-57 0.4490439969584692 1104 58-59 0.48892089967880636 1104 60-61 0.5571846976230432 1104 62-63 0.6146708381489923 1104 64-65 0.6256331779065754 1104 66-67 0.5904740829377708 1104 68-69 0.4282937423666944 1104 70-71 0.07062566336805531 1104 72-73 0.039450939267215546 1104 74-75 0.5285987755539843 1104 76-77 0.6273026409786517 1104 78-79 0.5978250914798515 1104 80-81 0.6139966797643304 1104 82-83 0.5910371568953714 1104 84-85 0.4992074276665086 1104 86-87 0.620329645259492 1104 88-89 0.629521771469669 1104 90-91 0.6696285238626274 1104 92-93 0.6122211481567419 1104 94-95 0.6337566466324205 1104 96-97 0.5577015660156022 1104 98-99 0.6482589872437678 1104 100-101 0.47115898680049284 1104 102-103 0.3796478183792438 1104 104-105 0.4737356879502137 1104 106-107 0.410811810397373 1104 108-109 0.5105502893996423 1104 110-111 0.4179510412270666 1104 112-113 0.29366134182380677 1104 114-115 0.2516566726758249 1104 116-117 0.3797977648394202 1104 118-119 0.3474738298510829 1104 120-121 0.1849884806551998 1104 122-123 0.2756433928654687 1104 124-125 0.724405679080725 1105 1 0.23718493582795475 1105 2 5.379543704435719E-4 1105 3 0.29844083741640404 1105 4 0.19517609264006808 1105 5 0.08577763252877446 1105 6 0.3445018561946753 1105 7 0.5553750976264809 1105 8 0.5488481174323141 1105 9 0.7773246874335769 1105 10-11 0.65854008036397 1105 12-13 0.7623982355758656 1105 14-15 0.7714798945285182 1105 16-17 0.6891497030289884 1105 18-19 0.7579707204968429 1105 20-21 0.7176256141071207 1105 22-23 0.8051798675374684 1105 24-25 0.6766950682148405 1105 26-27 0.8000578013562958 1105 28-29 0.7621154261709648 1105 30-31 0.8680411777973589 1105 32-33 1.0721562834119638 1105 34-35 1.034951652140336 1105 36-37 1.0310436369051743 1105 38-39 1.078135558380417 1105 40-41 0.9434000003954637 1105 42-43 0.9241228073344772 1105 44-45 1.0006428502783038 1105 46-47 0.8125793693517664 1105 48-49 0.8761164811936553 1105 50-51 0.6809602239063537 1105 52-53 0.8762640408007982 1105 54-55 0.7880867622959578 1105 56-57 0.8206414597056515 1105 58-59 0.4873670420771141 1105 60-61 0.5738328883555575 1105 62-63 0.7422391462823157 1105 64-65 0.5342995136517708 1105 66-67 0.713996895941424 1105 68-69 0.7392312086630568 1105 70-71 0.8520512158959725 1105 72-73 0.8471997685476005 1105 74-75 0.7807475705865095 1105 76-77 0.7892487582997987 1105 78-79 0.6332975617204077 1105 80-81 0.6206466265597257 1105 82-83 0.7294802847411148 1105 84-85 0.5802785783852684 1105 86-87 0.7122451790318074 1105 88-89 0.6980882855604555 1105 90-91 0.5998235024226481 1105 92-93 0.6874662592022425 1105 94-95 0.6611301866156687 1105 96-97 0.6606390079626401 1105 98-99 0.5703908317039712 1105 100-101 0.6284372454848324 1105 102-103 0.3235287067644457 1105 104-105 0.5916504803913902 1105 106-107 0.5602567979229711 1105 108-109 0.6582499330393006 1105 110-111 0.5389398038802824 1105 112-113 0.35618617912993855 1105 114-115 0.43150986740679187 1105 116-117 0.19285865125814539 1105 118-119 0.3235688779787189 1105 120-121 0.46946113973537607 1105 122-123 0.4936492041499818 1105 124-125 0.9209480217003723 1106 1 -0.0875258986669003 1106 2 -0.4589515713533423 1106 3 0.10742246992425919 1106 4 -0.19299748642168169 1106 5 0.2168724957076975 1106 6 0.3713642512491546 1106 7 0.4880114415646659 1106 8 0.4542361850251595 1106 9 0.5318322287967874 1106 10-11 0.5302156538630598 1106 12-13 0.5070737395383134 1106 14-15 0.4254838534096308 1106 16-17 0.32817725796596164 1106 18-19 0.5135617288306165 1106 20-21 0.45106536854189727 1106 22-23 0.4026703075941285 1106 24-25 0.4801022022977648 1106 26-27 0.5837618555577464 1106 28-29 0.5799608285951194 1106 30-31 0.5138425028671776 1106 32-33 0.4940035532379383 1106 34-35 0.5210524609039382 1106 36-37 0.6453121120984164 1106 38-39 0.6291499661844426 1106 40-41 0.6172987693009944 1106 42-43 0.3903346568356625 1106 44-45 0.4028598048719516 1106 46-47 0.25731838434438004 1106 48-49 0.4247730991653924 1106 50-51 0.4072082486016768 1106 52-53 0.3388148600487 1106 54-55 0.2994523718397737 1106 56-57 -0.0032701069011018546 1106 58-59 0.43381172231548604 1106 60-61 0.467070940796944 1106 62-63 0.3593552613117943 1106 64-65 0.43158871439598556 1106 66-67 0.2544254595497648 1106 68-69 0.24882867758307015 1106 70-71 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2216 74-75 -1.719350977401593 2216 76-77 -1.7851440030672947 2216 78-79 -1.7487263075204211 2216 80-81 -1.6254171474025476 2216 82-83 -1.5439234316090378 2216 84-85 -1.5567590464377083 2216 86-87 -1.8657852729523086 2216 88-89 -1.8271384493342495 2216 90-91 -1.7737083680443995 2216 92-93 -1.80061503829738 2216 94-95 -1.6951156036324804 2216 96-97 -1.814046201862304 2216 98-99 -1.9235027700932399 2216 100-101 -1.8287174517248346 2216 102-103 -1.7465525056011657 2216 104-105 -1.7675206887001487 2216 106-107 -1.7970011212991572 2216 108-109 -1.7876326151672401 2216 110-111 -1.8174060844150652 2216 112-113 -1.7024174417842701 2216 114-115 -1.8078017298849929 2216 116-117 -1.8679766970526508 2216 118-119 -1.9129050380440873 2216 120-121 -2.0636207581140624 2216 122-123 -2.121029652817363 2216 124-125 -2.402470318861205 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62091.0 3 20173.0 4 9447.0 5 7387.0 6 8120.0 7 10015.0 8 11272.0 9 11943.0 10 13492.0 11 14783.0 12 16709.0 13 20245.0 14 24153.0 15 20930.0 16 13103.0 17 11133.0 18 10269.0 19 9803.0 20 9606.0 21 9654.0 22 10836.0 23 13205.0 24 17296.0 25 23020.0 26 28534.0 27 31569.0 28 35198.0 29 41948.0 30 48705.0 31 56265.0 32 68517.0 33 85360.0 34 110439.0 35 151105.0 36 237213.0 37 372033.0 38 139608.0 39 582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87053988333937 20.207760409601462 14.082012222123815 22.839687484935354 2 15.286208673057969 21.192601469402383 39.47260550129917 24.04858435624048 3 19.052325591162536 26.52118620576886 29.667967885960106 24.758520317108506 4 12.242086080706198 17.419394980989107 37.194762040093565 33.14375689821113 5 12.209640595802014 39.884620618324625 33.93068837319216 13.975050412681203 6 30.437275760866093 38.279422610304515 17.246596829026952 14.036704799802438 7 27.575207759335168 33.841363707636134 20.771901529915105 17.81152700311359 8 26.34439223105015 36.165961309818826 19.107358168390718 18.38228829074031 9 26.81592778121561 16.033342946824327 19.822757093216328 37.32797217874373 10-11 24.662424562466505 27.863210339191006 26.747432029921335 20.72693306842115 12-13 25.710383416369826 25.361400545761725 27.7186588798837 21.209557157984747 14-15 22.793656482394052 26.082676283487142 26.310506451034854 24.813160783083955 16-17 21.267167108589923 29.578861601955474 27.050099257736925 22.10387203171768 18-19 21.120833637833126 27.35550539825633 30.459219287730882 21.06444167617966 20-21 22.081187858220844 26.387341906833882 30.77942695864728 20.752043276297996 22-23 21.75151098047275 26.426128692473405 30.556608639118 21.265751687935843 24-25 21.37892472732913 26.605268006188957 30.83175744122534 21.184049825256572 26-27 21.21610898658891 26.818062439486585 30.737819898631454 21.228008675293054 28-29 20.990042900477725 26.57357283533463 31.113934059485004 21.322450204702644 30-31 21.23520448705062 26.577072743777023 30.904919527305164 21.282803241867192 32-33 21.11153172233014 26.61489975422243 30.828873516668803 21.444695006778623 34-35 21.378644734653744 26.6205276069978 30.737511906688518 21.263315751659935 36-37 21.54490438530128 26.34143090816744 30.503634024933906 21.61003068159737 38-39 21.271351541443675 26.751592178348616 30.57206423479962 21.404992045408093 40-41 21.410199909170377 26.529613985298145 30.722784291962924 21.337401813568558 42-43 21.358149270815076 26.635283220990942 30.212217648386318 21.794349859807667 44-45 21.47588619081725 26.815570504675595 30.014458821757223 21.694084482749933 46-47 21.77057848166692 27.074227738202367 29.379575430306744 21.77561834982397 48-49 21.807145525073064 27.295673945169597 28.912071660205367 21.985108869551972 50-51 21.816749273838994 27.337028863324935 28.70874097933598 22.13748088350009 52-53 22.041247400967993 27.496624688298155 28.496702526261913 21.965425384471942 54-55 21.862892066743534 27.414978824153962 28.593104004399244 22.129025104703263 56-57 21.82406118286423 27.634775576585547 28.427395191974902 22.113768048575324 58-59 21.90039904891289 27.25297998707543 28.618683732176915 22.227937231834762 60-61 21.9480938378652 27.474029279394053 28.72640851715319 21.851468365587557 62-63 21.715027934869223 27.430602415440813 29.00620519767203 21.848164452017933 64-65 21.650868048993214 27.229257051979346 28.858845615936097 22.26102928309135 66-67 21.697640389727404 27.66862418879122 28.37056582599799 22.263169595483383 68-69 21.67779497866595 27.403422799417953 28.735908314692814 22.18287390722328 70-71 21.690052588224294 27.311241537921365 28.80553444721886 22.19317142663548 72-73 21.853484312850377 27.478957150480944 28.36451798420953 22.303040552459148 74-75 21.66853004035583 27.73892832633203 28.277444722480517 22.31509691083162 76-77 21.818317232821187 27.44012216640413 28.50812622741789 22.233434373356793 78-79 21.67644494420026 27.538987580084907 28.401393019558608 22.383174456156226 80-81 21.645349879043096 27.78878460711406 28.21986157154377 22.346003942299077 82-83 21.882799545963877 27.690211624063917 28.15799538684068 22.268993443131528 84-85 21.697584391192326 27.606157822911353 28.134280007235013 22.56197777866131 86-87 21.602218885953945 27.491752815746338 28.377481645080167 22.528546653219554 88-89 21.840716993885145 27.39087327420357 28.193148641720615 22.575261090190672 90-91 21.646105859689992 27.64397231430875 28.29445166203745 22.415470163963803 92-93 21.72266281686134 27.611834567384104 28.218705305092968 22.446797310661594 94-95 21.5523521904667 27.2620748241226 28.307763468907655 22.87780951650305 96-97 21.839800468139337 27.25448516854052 28.152428945336794 22.75328541798335 98-99 21.52167113788209 27.352498191866804 28.084151625023836 23.04167904522727 100-101 21.448743443095278 27.34801169056842 28.281700362824264 22.92154450351204 102-103 21.662893298711307 27.179868974627624 28.189410565019617 22.967827161641452 104-105 21.62965816814232 27.190144705814497 28.308463450596133 22.87173367544705 106-107 21.38945378375964 27.208024951133442 28.401878110946754 23.000643154160162 108-109 21.55319216849287 27.398431257038315 27.76292572186312 23.285450852605695 110-111 21.362845000747583 27.396588673598927 27.971358925094513 23.269207400558976 112-113 21.25124806735056 27.334704924119187 28.252016927237182 23.16203008129307 114-115 21.269811581729023 27.5091683601557 27.91095784934266 23.31006220877262 116-117 21.125279362691874 27.365056130131638 27.97068028700369 23.5389842201728 118-119 21.166578282312134 27.231023636421668 27.96732037489899 23.635077706367202 120-121 21.020253765661558 27.3899869074032 28.016141189488742 23.573618137446505 122-123 21.071302267482835 27.464545992416806 27.9009396253539 23.563212114746467 124-125 20.941575274400993 27.422668046962183 27.811254490210796 23.82450218842603 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 515.0 1 399.5 2 476.5 3 733.5 4 566.0 5 406.5 6 841.5 7 2071.0 8 3436.5 9 3851.5 10 2971.0 11 1897.5 12 1616.5 13 1695.5 14 1877.0 15 2039.0 16 2261.0 17 2556.0 18 2881.5 19 3287.5 20 3739.0 21 4315.5 22 5095.0 23 6077.5 24 7268.5 25 8906.5 26 11491.5 27 14291.5 28 16472.5 29 19306.5 30 23561.0 31 27559.5 32 31981.0 33 37436.0 34 43228.0 35 47866.0 36 53154.5 37 58483.5 38 59530.5 39 59607.0 40 58588.5 41 57189.0 42 56274.0 43 57006.5 44 59709.0 45 61101.5 46 59859.0 47 58219.5 48 59781.5 49 60498.5 50 59824.0 51 57864.5 52 55781.5 53 55228.0 54 53712.0 55 50014.5 56 44726.5 57 40278.0 58 38227.0 59 35710.0 60 31072.5 61 25412.5 62 19427.0 63 14360.5 64 10597.0 65 7985.5 66 5790.5 67 4260.5 68 3178.5 69 2240.5 70 1562.5 71 1133.0 72 879.5 73 801.5 74 634.0 75 450.0 76 325.0 77 184.0 78 120.0 79 79.5 80 50.5 81 41.0 82 32.0 83 22.5 84 14.0 85 5.5 86 2.5 87 4.5 88 4.0 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09984538804464875 2 5.599853507832234E-5 3 0.0 4 0.09990138657972707 5 0.0 6 0.0 7 0.0022959399382112165 8 0.007839794910965129 9 0.004255888665952498 10-11 6.719824209398681E-4 12-13 0.0 14-15 5.599853507832234E-5 16-17 3.3599121046993407E-4 18-19 0.002547933346063667 20-21 0.001455961912036381 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.009127761217766543 58-59 8.399780261748353E-4 60-61 0.0 62-63 0.0 64-65 1.9599487277412826E-4 66-67 0.0 68-69 2.799926753916117E-5 70-71 0.0 72-73 0.0 74-75 0.0012039685041839306 76-77 0.0 78-79 0.0 80-81 5.599853507832234E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0015399597146538648 90-91 5.599853507832234E-5 92-93 0.0024079370083678613 94-95 0.0 96-97 0.010163734116715506 98-99 0.004451883538726627 100-101 0.0026879296837594726 102-103 0.0 104-105 0.0 106-107 0.0017079553198888317 108-109 0.0 110-111 2.2399414031328937E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010639721664881246 122-123 0.005739849845528041 124-125 0.0897096531954724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1785761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.110297257396944 #Duplication Level Percentage of deduplicated Percentage of total 1 77.24566809204418 39.480490580306 2 11.781056773145286 12.042666273634502 3 4.309378901089382 6.607609098882987 4 2.073873179170796 4.239850986462488 5 1.1300636230385277 2.8878943846635057 6 0.7103947877863874 2.17850932643206 7 0.47657872698064313 1.705065628177274 8 0.3585960379267195 1.466236007500754 9 0.2548414923425755 1.1722521984432914 >10 1.4408450082505793 13.836372555279489 >50 0.13613751668207788 4.811975243019797 >100 0.07893812071322034 7.18254034399156 >500 0.0029681515879174305 0.9568533195103602 >1k 4.397261611729527E-4 0.5513003388746714 >5k 2.1986308058647634E-4 0.8803837148213645 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8330 0.46646779720242515 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7339 0.41097324893980774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4947 0.27702475303246066 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2217 0.12414875226864067 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.599853507832235E-5 0.0 0.0 0.0 0.0 3 5.599853507832235E-5 0.0 0.0 0.0 0.0 4 5.599853507832235E-5 0.0 0.0 5.599853507832235E-5 0.0 5 5.599853507832235E-5 0.0 0.0 5.599853507832235E-5 0.0 6 5.599853507832235E-5 0.0 0.0 5.599853507832235E-5 0.0 7 2.239941403132894E-4 0.0 0.0 5.599853507832235E-5 0.0 8 2.239941403132894E-4 0.0 0.0 5.599853507832235E-5 0.0 9 2.239941403132894E-4 0.0 0.0 5.599853507832235E-5 0.0 10-11 2.239941403132894E-4 0.0 0.0 1.119970701566447E-4 0.0 12-13 3.0799194293077293E-4 0.0 0.0 2.239941403132894E-4 0.0 14-15 4.479882806265788E-4 0.0 0.0 2.7999267539161174E-4 0.0 16-17 6.159838858615459E-4 0.0 0.0 3.0799194293077293E-4 0.0 18-19 7.839794910965129E-4 0.0 0.0 4.7598754816574E-4 0.0 20-21 8.399780261748353E-4 0.0 0.0 6.159838858615459E-4 0.0 22-23 9.5197509633148E-4 0.0 0.0 0.0012039685041839306 5.599853507832235E-5 24-25 0.0012879663068014141 0.0 0.0 0.0020159472628196048 5.599853507832235E-5 26-27 0.0013439648418797365 0.0 0.0 0.0024919348109853448 5.599853507832235E-5 28-29 0.0016519567848105093 0.0 0.0 0.003499908442395147 5.599853507832235E-5 30-31 0.0017359545874279928 0.0 0.0 0.0061038403235371365 5.599853507832235E-5 32-33 0.001763953854967154 0.0 0.0 0.011115709213046988 5.599853507832235E-5 34-35 0.0019319494602021213 0.0 0.0 0.01691155759365335 5.599853507832235E-5 36-37 0.002239941403132894 0.0 0.0 0.028223261679474466 5.599853507832235E-5 38-39 0.0025759326136028282 0.0 0.0 0.0499786925574027 5.599853507832235E-5 40-41 0.0025759326136028282 0.0 0.0 0.0762980040442142 5.599853507832235E-5 42-43 0.0026599304162203117 0.0 0.0 0.09886541368077811 5.599853507832235E-5 44-45 0.002743928218837795 0.0 0.0 0.12652869000946937 5.599853507832235E-5 46-47 0.0030519201617685682 0.0 0.0 0.16250774879729146 5.599853507832235E-5 48-49 0.003191916499464374 0.0 0.0 0.20089474459348144 5.599853507832235E-5 50-51 0.003191916499464374 0.0 0.0 0.24978146571685686 5.599853507832235E-5 52-53 0.003191916499464374 0.0 0.0 0.2937683150208791 5.599853507832235E-5 54-55 0.003191916499464374 0.0 0.0 0.3406950874165132 5.599853507832235E-5 56-57 0.0032479150345426965 0.0 0.0 0.3976735968587062 5.599853507832235E-5 58-59 0.0032479150345426965 0.0 0.0 0.46531982723331955 5.599853507832235E-5 60-61 0.0032479150345426965 0.0 0.0 0.5453697331277814 5.599853507832235E-5 62-63 0.0032759143020818574 0.0 0.0 0.6259236258379481 5.599853507832235E-5 64-65 0.0033039135696210187 0.0 0.0 0.7027536159654064 5.599853507832235E-5 66-67 0.0033599121046993413 0.0 0.0 0.7910073072488424 5.599853507832235E-5 68-69 0.0033599121046993413 0.0 0.0 0.9003164477217276 5.599853507832235E-5 70-71 0.0034439099073168243 0.0 0.0 1.0307090366516012 5.599853507832235E-5 72-73 0.0034719091748559856 0.0 0.0 1.161857605805032 5.599853507832235E-5 74-75 0.003499908442395147 0.0 0.0 1.2887782855600496 5.599853507832235E-5 76-77 0.0035279077099343082 0.0 0.0 1.4312665580668409 5.599853507832235E-5 78-79 0.0035839062450126304 0.0 0.0 1.6030420644195948 5.599853507832235E-5 80-81 0.00405989379317837 0.0 0.0 1.7986449474481747 5.599853507832235E-5 82-83 0.0041998901308741765 0.0 0.0 2.0040195748479217 5.599853507832235E-5 84-85 0.004311887201030821 0.0 0.0 2.206958265971762 5.599853507832235E-5 86-87 0.004479882806265788 0.0 0.0 2.4150768215903473 5.599853507832235E-5 88-89 0.004563880608883271 0.0 0.0 2.6562345129051423 5.599853507832235E-5 90-91 0.0051518652272056565 0.0 0.0 2.944234978812954 5.599853507832235E-5 92-93 0.005319860832440623 0.0 0.0 3.2484190213584014 5.599853507832235E-5 94-95 0.005319860832440623 0.0 0.0 3.5511191027242726 5.599853507832235E-5 96-97 0.005319860832440623 0.0 0.0 3.8553871430723374 5.599853507832235E-5 98-99 0.005683851310449718 0.0 0.0 4.1852185146836565 5.599853507832235E-5 100-101 0.005851846915684686 0.0 0.0 4.58196813571357 5.599853507832235E-5 102-103 0.006019842520919653 0.0 0.0 5.017776734960613 5.599853507832235E-5 104-105 0.006859820547094489 0.0 0.0 5.443197605950628 5.599853507832235E-5 106-107 0.007027816152329455 0.0 0.0 5.862262643209254 5.599853507832235E-5 108-109 0.007139813222486099 0.0 0.0 6.292527387483544 5.599853507832235E-5 110-111 0.0071958117575644225 0.0 0.0 6.795982217105201 5.599853507832235E-5 112-113 0.007279809560181906 0.0 0.0 7.342555918737165 5.599853507832235E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5750 0.0 60.348667 1 GTATCAA 9905 0.0 47.493595 1 ATCAACG 12495 0.0 37.901257 3 TCAACGC 12630 0.0 37.213146 4 CAACGCA 12760 0.0 36.79172 5 AACGCAG 13105 0.0 36.095573 6 TATCAAC 13205 0.0 35.457397 2 GTACATG 11120 0.0 31.312704 1 ACGCAGA 15575 0.0 30.218454 7 CGCAGAG 15620 0.0 30.05521 8 TACATGG 11360 0.0 29.994417 2 TATAACG 145 4.0318337E-6 28.757608 2 ACATGGG 11485 0.0 28.734356 3 GCAGAGT 16255 0.0 28.147438 9 GAGTACT 9545 0.0 27.739315 12-13 GTACTTT 10125 0.0 26.238443 14-15 TACCTGG 1605 0.0 25.980396 2 CATGGGA 7295 0.0 25.967165 4 AGTACTT 10145 0.0 24.573874 12-13 GAGTACA 8740 0.0 24.5586 1 >>END_MODULE