FastQCFastQC Report
Thu 26 May 2016
SRR1364562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455399
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.17019387810490455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.12951774736687327No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14700.10100323004207094No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55100.051.073891
GGTATCA31150.044.884791
ATCAACG65500.041.7836043
TCAACGC65550.041.751734
CAACGCA67150.040.7569055
TATCAAC67400.040.2526362
AACGCAG69550.039.2595446
GTACATG90950.038.857341
TACATGG94400.036.4918562
ACATGGG95350.034.5683333
ACGCAGA80350.033.4643367
CGCAGAG80050.032.9254538
CATGGGG35300.031.5178284
GCAGAGT84400.029.8890979
CATGGGA57300.029.8000264
ATGGGAT23400.026.4427645
GAGTACT33500.025.49182512-13
AGTACAT63800.024.246112
AGAGTAC79550.023.26398810-11
CGTAACG902.1807642E-523.13583100-101
Produced by FastQC (version 0.11.5)