##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364562_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1455399 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.952791639955777 31.0 30.0 33.0 23.0 34.0 2 28.970243898752162 31.0 30.0 34.0 22.0 34.0 3 29.129799457056105 31.0 30.0 34.0 23.0 34.0 4 32.49588394660159 35.0 33.0 37.0 24.0 37.0 5 32.62668656499008 35.0 33.0 37.0 25.0 37.0 6 32.74000806651647 35.0 33.0 37.0 25.0 37.0 7 32.607950809365676 35.0 33.0 37.0 25.0 37.0 8 32.532021802955754 35.0 33.0 37.0 24.0 37.0 9 33.68724521591673 38.0 33.0 39.0 21.0 39.0 10-11 33.629937907061915 38.0 33.0 39.0 20.0 39.0 12-13 33.544105774430236 38.0 33.0 39.0 18.5 39.0 14-15 34.33715874478408 39.0 33.0 41.0 17.5 41.0 16-17 34.461002790300114 39.0 33.0 41.0 18.0 41.0 18-19 34.30969239363226 39.0 33.0 41.0 17.5 41.0 20-21 34.07765705486949 39.0 33.0 41.0 18.0 41.0 22-23 33.97683693612542 39.0 32.5 41.0 17.5 41.0 24-25 33.83871124001047 38.5 32.0 41.0 17.0 41.0 26-27 33.76992460486781 38.0 32.0 41.0 16.5 41.0 28-29 33.82853945893875 38.0 32.5 40.5 16.5 41.0 30-31 33.78566255714068 38.0 32.5 40.0 16.5 41.0 32-33 33.76462021754859 38.0 33.0 40.0 16.5 41.0 34-35 33.37203680914993 38.0 32.0 40.0 15.0 41.0 36-37 33.20184636652904 38.0 32.0 40.0 12.5 41.0 38-39 33.01998352341866 38.0 31.0 40.0 9.0 41.0 40-41 33.198347326059725 38.0 32.0 40.0 13.5 41.0 42-43 33.061355339669745 38.0 32.0 40.0 9.0 41.0 44-45 32.895349660127565 38.0 32.0 40.0 9.0 41.0 46-47 32.821632418326516 38.0 32.0 40.0 8.0 41.0 48-49 32.642066883377 38.0 31.5 40.0 6.0 41.0 50-51 32.47615190061282 38.0 31.0 40.0 2.0 41.0 52-53 32.42167405639278 37.5 31.0 40.0 2.0 41.0 54-55 32.4508773882626 38.0 31.0 40.0 2.0 41.0 56-57 32.32445192005766 37.0 31.0 40.0 2.0 41.0 58-59 32.11521273547666 37.0 31.0 40.0 2.0 41.0 60-61 31.90783455258661 36.5 31.0 40.0 2.0 41.0 62-63 31.616724004894877 36.0 30.5 40.0 2.0 41.0 64-65 31.33029361707683 36.0 30.0 39.0 2.0 41.0 66-67 31.063452015564117 35.0 30.0 39.0 2.0 41.0 68-69 31.011253271439656 35.0 30.0 39.0 2.0 41.0 70-71 30.773838995354538 35.0 30.0 38.5 2.0 40.0 72-73 30.48666619944084 35.0 30.0 37.5 2.0 40.0 74-75 30.173544849213172 35.0 30.0 37.0 2.0 39.5 76-77 29.865796252436617 35.0 30.0 36.5 2.0 39.0 78-79 29.550130582747414 35.0 29.5 36.0 2.0 39.0 80-81 29.25053026695772 34.0 29.0 36.0 2.0 37.5 82-83 28.99610690951416 34.0 29.0 35.0 2.0 37.0 84-85 28.746879034546538 34.0 29.0 35.0 2.0 37.0 86-87 28.546497214853108 34.0 29.0 35.0 2.0 36.0 88-89 28.3439506966818 34.0 29.0 35.0 2.0 36.0 90-91 28.193249067781416 34.0 29.0 35.0 2.0 36.0 92-93 28.069540380335564 34.0 29.0 35.0 2.0 35.5 94-95 27.946139855805868 34.0 28.5 35.0 2.0 35.0 96-97 27.82796745085025 34.0 28.0 35.0 2.0 35.0 98-99 27.73678455186516 34.0 28.0 35.0 2.0 35.0 100-101 27.646930154548684 34.0 27.5 35.0 2.0 35.0 102-103 26.46174382420216 32.0 25.0 34.0 2.0 35.0 104-105 27.34894279850405 34.0 27.0 35.0 2.0 35.0 106-107 27.493637483604154 34.0 27.0 35.0 2.0 35.0 108-109 27.451076302787072 34.0 27.0 35.0 2.0 35.0 110-111 27.3633251087846 34.0 27.0 35.0 2.0 35.0 112-113 27.27159321945391 34.0 27.0 35.0 2.0 35.0 114-115 27.154584756482585 34.0 27.0 35.0 2.0 35.0 116-117 27.04213792918643 34.0 27.0 35.0 2.0 35.0 118-119 26.909531681690037 34.0 26.0 35.0 2.0 35.0 120-121 26.737933034171384 34.0 25.5 35.0 2.0 35.0 122-123 26.50788512291131 34.0 25.0 35.0 2.0 35.0 124-125 25.041261537214194 32.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8777871242989725 1101 2 0.9619592070614047 1101 3 0.8836362252288765 1101 4 0.9724505665118528 1101 5 0.9913143566929534 1101 6 0.966564147352706 1101 7 0.9930971913877045 1101 8 0.9520132325286212 1101 9 1.2715935949595334 1101 10-11 1.4190417832527586 1101 12-13 1.2392405707055616 1101 14-15 1.410405033281286 1101 16-17 1.2797809272996261 1101 18-19 1.685515419350466 1101 20-21 1.9810377062998938 1101 22-23 1.8775995071714675 1101 24-25 2.0265152723439215 1101 26-27 2.0897717354250247 1101 28-29 1.9483044964797145 1101 30-31 1.978086541725574 1101 32-33 1.9792729904211441 1101 34-35 2.2180826392578865 1101 36-37 2.2234448181597273 1101 38-39 2.09540520147214 1101 40-41 1.830344510822293 1101 42-43 1.9026645277753857 1101 44-45 1.8696688668009713 1101 46-47 1.6835885188482251 1101 48-49 1.5425388326254534 1101 50-51 1.5366248470694686 1101 52-53 1.3812698536650885 1101 54-55 1.2951928328282136 1101 56-57 1.424952880872631 1101 58-59 1.470791350739347 1101 60-61 1.4213010052306778 1101 62-63 1.3461970029616843 1101 64-65 1.519896988766856 1101 66-67 1.51463947306312 1101 68-69 1.394205143728243 1101 70-71 1.2357115414540054 1101 72-73 1.1850792126932888 1101 74-75 1.2747191991994988 1101 76-77 1.4034822743554507 1101 78-79 1.491931835536402 1101 80-81 1.4469236595422892 1101 82-83 1.2727086806243584 1101 84-85 1.358845702861121 1101 86-87 1.325340573126276 1101 88-89 1.2891481449136322 1101 90-91 1.2304214437221326 1101 92-93 1.148290559065888 1101 94-95 1.1546902967807746 1101 96-97 1.1686908267016456 1101 98-99 1.2423229035763619 1101 100-101 1.24460180214243 1101 102-103 1.1565697729662858 1101 104-105 1.2791375458893661 1101 106-107 1.2256441337354183 1101 108-109 1.2349755899891512 1101 110-111 1.108337900671227 1101 112-113 1.122108857023914 1101 114-115 1.2119626959238836 1101 116-117 1.2544724100514202 1101 118-119 1.2016087158764748 1101 120-121 1.2225298782479896 1101 122-123 1.372193012105413 1101 124-125 1.5837834655069507 1102 1 0.12401446859785636 1102 2 0.5132390861556964 1102 3 0.5586529988627582 1102 4 0.7686616454687893 1102 5 0.6770702093922125 1102 6 0.15111817200825328 1102 7 -0.3731632935453959 1102 8 0.3365021958163936 1102 9 0.7995812784811562 1102 10-11 0.8738153787291907 1102 12-13 0.870565416693104 1102 14-15 1.0859498657595665 1102 16-17 1.0473054213232444 1102 18-19 1.1053939262824315 1102 20-21 1.3885229262999346 1102 22-23 1.2815972797232575 1102 24-25 1.4998948941295396 1102 26-27 0.9577019593671494 1102 28-29 1.3154753887466697 1102 30-31 1.1840568823263595 1102 32-33 1.1721127383987024 1102 34-35 1.6043204091891425 1102 36-37 1.6734661664210293 1102 38-39 1.6132259167405536 1102 40-41 1.4512982246290775 1102 42-43 1.242579959292641 1102 44-45 1.389565107473807 1102 46-47 1.168367434446914 1102 48-49 0.8082091906211133 1102 50-51 1.1142446966602364 1102 52-53 0.9181229956999175 1102 54-55 0.8776013572228365 1102 56-57 0.829780016432963 1102 58-59 0.9156456290890489 1102 60-61 0.725800066584906 1102 62-63 0.6303434904385021 1102 64-65 0.7351498696525915 1102 66-67 0.7122597066966847 1102 68-69 0.5034545885604942 1102 70-71 0.6013344023921086 1102 72-73 0.7312355090749278 1102 74-75 0.45108944130459605 1102 76-77 0.7725276343277976 1102 78-79 0.6306511996518971 1102 80-81 0.34595287842183 1102 82-83 0.482307378493374 1102 84-85 0.11536285263103707 1102 86-87 0.5059245553789786 1102 88-89 0.8304759502897809 1102 90-91 0.39155309359257373 1102 92-93 0.6077785622466614 1102 94-95 0.5884581632423185 1102 96-97 0.7519685303064527 1102 98-99 0.8149309946803491 1102 100-101 0.791519971658925 1102 102-103 0.6454669827824198 1102 104-105 0.8171100597205552 1102 106-107 0.6577185408975872 1102 108-109 0.2945979516716193 1102 110-111 0.5319334335757873 1102 112-113 0.16600212475370313 1102 114-115 0.3003975859859871 1102 116-117 0.19683436360934792 1102 118-119 0.5544302444119822 1102 120-121 0.8421866004653182 1102 122-123 0.47888264227220034 1102 124-125 0.8250323726792885 1103 1 0.8512392176228367 1103 2 0.881413145731706 1103 3 0.7797921253354687 1103 4 1.0760512231788155 1103 5 0.9728774457338005 1103 6 1.1208574926620827 1103 7 1.1923510639882693 1103 8 1.355668731394715 1103 9 1.5748871497056456 1103 10-11 1.563017220137091 1103 12-13 1.5198108684008176 1103 14-15 1.8896549618496792 1103 16-17 1.6611849415758684 1103 18-19 1.7185967088762482 1103 20-21 2.0609147935601655 1103 22-23 2.0120001402318195 1103 24-25 2.08371003628136 1103 26-27 1.9143730499104876 1103 28-29 1.9958980970716098 1103 30-31 1.9552506494494821 1103 32-33 1.8117230548715142 1103 34-35 2.25125416291381 1103 36-37 2.207645298784662 1103 38-39 2.2994102653791373 1103 40-41 1.9867216077986996 1103 42-43 1.8627897687895754 1103 44-45 1.4873354319879724 1103 46-47 1.6808142016947798 1103 48-49 1.5907198097234385 1103 50-51 1.637050755762246 1103 52-53 1.604759102218999 1103 54-55 1.3846191740690372 1103 56-57 1.3968866477616189 1103 58-59 1.3996201027338557 1103 60-61 1.4016029755914197 1103 62-63 1.4615369130406286 1103 64-65 1.5408891539613485 1103 66-67 1.6347090729957543 1103 68-69 1.667090606299162 1103 70-71 1.5262051644289976 1103 72-73 1.4643321640600604 1103 74-75 1.3604890339747797 1103 76-77 1.3527825685025192 1103 78-79 1.3814446863879333 1103 80-81 1.2802862312527807 1103 82-83 1.3941684979177893 1103 84-85 1.307538648441394 1103 86-87 1.4443808022543934 1103 88-89 1.4398104436366452 1103 90-91 1.477553293869061 1103 92-93 1.5036698087065687 1103 94-95 1.3192149149545571 1103 96-97 1.3515739969837917 1103 98-99 1.3444851220512746 1103 100-101 1.2633609650000146 1103 102-103 1.3148264027907963 1103 104-105 1.2462118045300699 1103 106-107 1.3259027089465967 1103 108-109 1.436236114905288 1103 110-111 1.5026296518143134 1103 112-113 1.4698629178296656 1103 114-115 1.4444979385142496 1103 116-117 1.4758197000766877 1103 118-119 1.4942165883949912 1103 120-121 1.4807116758198156 1103 122-123 1.5318287037252816 1103 124-125 1.6661874682677755 1104 1 0.4480076243391906 1104 2 0.6848720342326828 1104 3 0.47098897190511124 1104 4 0.6272401568256782 1104 5 0.5539332787228091 1104 6 0.5573324877724275 1104 7 0.6302676354415837 1104 8 0.5064961615003583 1104 9 0.8412010214601082 1104 10-11 0.790313869026825 1104 12-13 0.8189121020974213 1104 14-15 0.807873867078392 1104 16-17 0.9746985387579983 1104 18-19 1.0909533193609278 1104 20-21 1.392147915745035 1104 22-23 1.138863332024279 1104 24-25 1.4644770350629273 1104 26-27 1.6189523452881502 1104 28-29 1.508930262154287 1104 30-31 1.551366803711744 1104 32-33 1.4382169948288563 1104 34-35 1.8131662521669938 1104 36-37 1.9403756848196991 1104 38-39 1.8204300625384704 1104 40-41 1.4506042689242946 1104 42-43 1.443575574630927 1104 44-45 1.296621288023239 1104 46-47 1.302655586424052 1104 48-49 1.1280358973468054 1104 50-51 0.8470576147023223 1104 52-53 1.0628334955034262 1104 54-55 0.9837745205618305 1104 56-57 0.9018076196374594 1104 58-59 0.9040364513118817 1104 60-61 0.9845362040871741 1104 62-63 0.9576130195390498 1104 64-65 0.9459160967297287 1104 66-67 0.9295390820485565 1104 68-69 0.8805098460823757 1104 70-71 0.871407583686139 1104 72-73 0.8262135464487415 1104 74-75 0.7948517690115793 1104 76-77 0.7405436998751149 1104 78-79 0.790945418896964 1104 80-81 0.8046285791504637 1104 82-83 0.7882526307897564 1104 84-85 0.7274291630802097 1104 86-87 0.7599793704842064 1104 88-89 0.850903692116912 1104 90-91 0.8700903626554783 1104 92-93 0.8422339263754175 1104 94-95 1.0508630450657677 1104 96-97 0.9549935892875361 1104 98-99 0.9559605242602025 1104 100-101 0.957852625921074 1104 102-103 0.8803554958449453 1104 104-105 0.8912151302123377 1104 106-107 0.8572352526578015 1104 108-109 0.9854326812479499 1104 110-111 0.9385945658216706 1104 112-113 1.0017597628066994 1104 114-115 1.107759769827986 1104 116-117 1.019559713858989 1104 118-119 1.0438800207603478 1104 120-121 1.0416357233925524 1104 122-123 1.1057632641044428 1104 124-125 1.3903750471263479 1105 1 0.057154864501104186 1105 2 0.2400080363475432 1105 3 0.35958314295879035 1105 4 0.44976798545363295 1105 5 0.4126170045184452 1105 6 0.3408503148653992 1105 7 0.4950857757842826 1105 8 0.3249674843692958 1105 9 0.27129562884621095 1105 10-11 0.6141449209717038 1105 12-13 0.5705495922905968 1105 14-15 0.7217074524517315 1105 16-17 0.5332332612960613 1105 18-19 0.7367077125319668 1105 20-21 0.9728300617898995 1105 22-23 1.1416521464223948 1105 24-25 1.1750710298780973 1105 26-27 1.0827534544536874 1105 28-29 0.7426921582531563 1105 30-31 0.6778899698364285 1105 32-33 0.7956594117409352 1105 34-35 1.3158462499498 1105 36-37 1.3124059239355503 1105 38-39 1.509809969501596 1105 40-41 1.0518858888119595 1105 42-43 0.7793044132192009 1105 44-45 0.9045884230958379 1105 46-47 0.7835297116272955 1105 48-49 0.721151296612291 1105 50-51 0.7058067192970299 1105 52-53 0.7256307913794444 1105 54-55 0.44179609059104763 1105 56-57 0.4879055464188866 1105 58-59 0.46669163712979866 1105 60-61 0.47339755768414804 1105 62-63 0.48545257357036675 1105 64-65 0.5448804112756598 1105 66-67 0.4518265014620795 1105 68-69 0.3251363128415221 1105 70-71 0.43820952643136835 1105 72-73 0.5306777458711345 1105 74-75 0.6692559869390138 1105 76-77 0.7550376288851091 1105 78-79 0.7285976733285082 1105 80-81 0.6250538042809346 1105 82-83 0.49296244485813645 1105 84-85 0.6539175434199542 1105 86-87 0.6687347287031749 1105 88-89 0.7442277883260786 1105 90-91 0.6043485513500642 1105 92-93 0.47820264119878075 1105 94-95 0.5428890523256591 1105 96-97 0.5904250609358748 1105 98-99 0.44154218948663626 1105 100-101 0.4591641992476596 1105 102-103 0.43578152755081945 1105 104-105 0.4466477353300746 1105 106-107 0.42784879715518187 1105 108-109 0.45097565572747556 1105 110-111 0.600869097794746 1105 112-113 0.6565657441140509 1105 114-115 0.5646699002729036 1105 116-117 0.585113330159345 1105 118-119 0.6679163941130852 1105 120-121 0.5110907662298771 1105 122-123 0.7163745985141574 1105 124-125 0.7295271231874736 1106 1 0.45342106457489706 1106 2 0.6607227997457557 1106 3 0.5217712482996255 1106 4 0.5046868121301387 1106 5 0.5330279444686354 1106 6 0.286296133978027 1106 7 0.20830843743911487 1106 8 0.3795821993402413 1106 9 0.5875184749858633 1106 10-11 0.6084525551844067 1106 12-13 0.6881745231087919 1106 14-15 0.8516078306456265 1106 16-17 0.8188698204234441 1106 18-19 0.8499280681264736 1106 20-21 1.1494159106507098 1106 22-23 1.1261561416719132 1106 24-25 1.2718650295091294 1106 26-27 1.413405031798952 1106 28-29 1.3691449762588732 1106 30-31 1.1802313417117176 1106 32-33 1.1140787803416217 1106 34-35 1.5516913634079685 1106 36-37 1.395885307756231 1106 38-39 1.4897940865110897 1106 40-41 1.2891542738779052 1106 42-43 1.1512070859431205 1106 44-45 1.071269742854163 1106 46-47 0.8310698659027338 1106 48-49 0.892678946275602 1106 50-51 0.8339314310394883 1106 52-53 0.7058289396130064 1106 54-55 0.5124357628998766 1106 56-57 0.6178455600246409 1106 58-59 0.6361243751152301 1106 60-61 0.6865214911312485 1106 62-63 0.5487200592727923 1106 64-65 0.5511240696097595 1106 66-67 0.4601196709113893 1106 68-69 0.4709009447942911 1106 70-71 0.6580340064147627 1106 72-73 0.644598567289826 1106 74-75 0.6261204078872673 1106 76-77 0.4856429408950689 1106 78-79 0.44644398593804624 1106 80-81 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-1.4149915460567613 2216 112-113 -1.4675230343395782 2216 114-115 -1.6010915869974305 2216 116-117 -1.5432258558944625 2216 118-119 -1.568781730926105 2216 120-121 -1.6031367334565836 2216 122-123 -1.5831547016676915 2216 124-125 -1.7595210716140564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68615.0 3 12234.0 4 7838.0 5 6407.0 6 6323.0 7 6604.0 8 6837.0 9 7410.0 10 7861.0 11 8870.0 12 9838.0 13 11027.0 14 12944.0 15 11660.0 16 8345.0 17 7542.0 18 6954.0 19 6564.0 20 6335.0 21 6663.0 22 8145.0 23 10623.0 24 14536.0 25 18246.0 26 22205.0 27 24913.0 28 29321.0 29 34961.0 30 39691.0 31 45583.0 32 57393.0 33 74001.0 34 97580.0 35 135748.0 36 213070.0 37 295395.0 38 106744.0 39 373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82092779870964 19.261419507700044 13.172366828815099 22.745285864775216 2 15.090000329822557 20.203280635018363 41.28395798060643 23.42276105455265 3 19.345859190274332 26.738029647841692 28.247083213368125 25.669027948515854 4 11.972612981073162 16.785131190527235 37.91215209635481 33.33010373204479 5 13.173541179041523 39.49141756838663 33.561987435248646 13.773053817323195 6 31.047253378480516 36.9392217058269 18.11242088020514 13.901104035487444 7 28.504531940024886 33.11727799128312 20.78453306691165 17.593657001780343 8 26.21720916394748 35.657919237267585 19.56439436882944 18.560477229955495 9 27.30145467862878 16.716058357548203 19.776442190421285 36.206044773401736 10-11 24.85043620340539 27.539698735535755 27.057116295943583 20.55274876511527 12-13 25.651741033729543 25.084394288466655 27.411292378369502 21.852572299434303 14-15 22.78837610267666 26.148202766750344 25.536881643805113 25.52653948676788 16-17 22.057238798071836 29.518982696269987 25.98208873573772 22.441689769920455 18-19 22.069864932926833 28.20230308702162 28.196943555197336 21.53088842485421 20-21 23.15758516303898 27.127010265499862 28.224163692790167 21.491240878670993 22-23 22.662883873565477 27.27812736624977 27.949712854946057 22.109275905238697 24-25 22.188310849440377 27.436808716494056 28.243672625722894 22.13120780834268 26-27 22.076764125614773 27.49395104902313 28.23246938072898 22.196815444633113 28-29 22.097223744336162 27.389393467274015 28.336867882304155 22.176514906085664 30-31 22.21669484939703 27.36558005693347 28.21666186838791 22.201063225281594 32-33 22.05106040580204 27.36135222912145 28.117236434298952 22.470350930777553 34-35 22.438108156207463 27.319058819325768 28.19814322750788 22.044689796958885 36-37 22.469632497822612 27.04271806967304 28.105559172609034 22.382090259895314 38-39 22.234700667892675 27.469108975995233 28.090313409007912 22.205876947104176 40-41 22.414367175272183 27.307597813929757 28.100074048748475 22.177960962049585 42-43 22.238133341670636 27.40491435996472 27.79881763738429 22.55813466098035 44-45 22.323386417907884 27.442560428799673 27.80965142847326 22.424401724819187 46-47 22.46079511216071 27.623376096888276 27.342622170215485 22.573206620735526 48-49 22.410304251689283 27.643316479140147 27.25991365017842 22.68646561899215 50-51 22.478171021010446 27.82689357696358 27.105210267650854 22.589725134375126 52-53 22.568347236737143 27.872425362392033 27.086524039112298 22.472703361758526 54-55 22.478098318680672 27.725637053140247 27.197186084490994 22.599078543688087 56-57 22.43841201849443 27.949465320192914 27.05315602975765 22.55896663155501 58-59 22.483900391131602 27.67284426709633 27.17445637517027 22.668798966601795 60-61 22.49891092687338 27.790775653684364 27.26740215176114 22.44291126768112 62-63 22.411080911130735 27.64107991464592 27.432333526903374 22.515505647319973 64-65 22.333973329081967 27.620707000600596 27.282956917795136 22.762362752522304 66-67 22.391972111683813 27.899584746627422 26.953025557304244 22.75541758438452 68-69 22.501700808829085 27.677725932696074 27.23225145857242 22.58832179990242 70-71 22.32117734725828 27.599994090863962 27.278701996429096 22.800126565448664 72-73 22.430957829131366 27.81607536745759 26.981075676678557 22.771891126732495 74-75 22.346930291968047 27.945429397711557 27.016543229726008 22.691097080594393 76-77 22.517753522360678 27.723769965197253 27.18132126746579 22.577155244976275 78-79 22.373907935013758 27.752381672203576 27.063113883955104 22.81059650882756 80-81 22.278572859232163 27.852957131564004 27.074702232385338 22.7937677768185 82-83 22.497056613678268 28.032718568980027 26.88892912270769 22.58129569463402 84-85 22.32951238801181 27.935775687629306 26.861121932885755 22.87358999147313 86-87 22.243519093662563 27.909708441669096 27.116395208856687 22.73037725581165 88-89 22.508119013816412 27.776922622328076 26.881597772442216 22.833360591413292 90-91 22.350881991350857 27.946677130485515 27.06403293629005 22.638407941873577 92-93 22.399009454024746 27.891262568204823 27.11980342994409 22.589924547826342 94-95 22.226689193948694 27.77487424615557 27.136990683615043 22.86144587628069 96-97 22.525132547366077 27.620977924863656 26.98489487433125 22.868994653439014 98-99 22.238227881499643 27.6536222643034 26.930481768435634 23.177668085761322 100-101 22.26041163483745 27.658231631690374 27.07698449618262 23.004372237289555 102-103 22.310101903326853 27.489746797957125 27.18175565600911 23.018395642706917 104-105 22.250591939183145 27.521770856666205 27.16379022472389 23.063846979426764 106-107 22.107347231703205 27.74772682533341 27.107730288302484 23.037195654660906 108-109 22.20676254415456 27.798356327027847 26.814742898682763 23.18013823013483 110-111 22.060184596695454 27.805109149512603 26.916274617988726 23.21843163580321 112-113 22.10984754009038 27.788015520142583 27.093463716822676 23.008673222944363 114-115 22.117165121042408 27.990537302828983 26.949585646272944 22.942711929855662 116-117 21.88049462724655 27.937699558677725 27.038667746782842 23.143138067292888 118-119 21.890423176050003 27.976726657088534 26.92409435488138 23.208755811980083 120-121 21.836760915735134 28.066014886639334 26.929110161543328 23.1681140360822 122-123 21.834459141445063 28.078485693613914 26.933438871402277 23.153616293538747 124-125 21.91703577454238 27.918472116749193 26.863619651085674 23.300872457622752 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1236.0 1 1181.5 2 1322.5 3 1297.0 4 907.5 5 647.5 6 535.5 7 588.0 8 774.0 9 876.0 10 783.5 11 685.5 12 678.0 13 739.0 14 832.5 15 938.5 16 1089.0 17 1198.5 18 1328.5 19 1581.0 20 1905.5 21 2331.5 22 2901.5 23 3538.5 24 4399.0 25 5790.0 26 7760.5 27 9993.0 28 11736.5 29 13875.0 30 17210.0 31 20630.0 32 24732.5 33 29087.5 34 33098.5 35 36463.5 36 40337.0 37 44146.5 38 44976.0 39 45267.0 40 45379.0 41 45398.0 42 45546.0 43 46298.0 44 47542.5 45 48291.0 46 48876.5 47 48438.5 48 49371.0 49 50160.5 50 49316.0 51 48395.0 52 47176.5 53 46783.5 54 46502.0 55 44732.0 56 41863.0 57 38756.0 58 36339.5 59 32865.5 60 28033.5 61 22997.0 62 18330.5 63 14601.0 64 10912.0 65 7837.5 66 5927.0 67 4679.5 68 3615.0 69 2794.5 70 2134.0 71 1593.5 72 1251.0 73 1038.5 74 795.0 75 529.0 76 370.0 77 290.0 78 227.5 79 169.5 80 110.0 81 77.0 82 58.5 83 39.0 84 28.5 85 25.5 86 23.0 87 17.5 88 14.5 89 13.5 90 11.0 91 7.0 92 7.5 93 6.0 94 3.5 95 2.5 96 2.0 97 4.0 98 4.0 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010169032684507822 2 0.004878387301351725 3 0.007970322914884509 4 0.007626774513380867 5 0.014910000625258091 6 7.558064833080138E-4 7 0.004260000178645169 8 0.0 9 0.0017864516878189417 10-11 0.0 12-13 0.001923871048420399 14-15 0.01305483925713842 16-17 0.023155162261345515 18-19 0.0032980646544349696 20-21 0.006012097026313746 22-23 0.0025422581711269554 24-25 0.00927580684059835 26-27 0.012917419896536964 28-29 2.4048388105254987E-4 30-31 0.0010993548848116565 32-33 0.005737258305110832 34-35 0.005359355063456825 36-37 0.006974032550523946 38-39 3.435484015036427E-5 40-41 0.01899822660315144 42-43 0.006355645427817389 44-45 0.011955484372326764 46-47 0.002198709769623313 48-49 0.018654678201647797 50-51 0.0035385485354875196 52-53 0.0 54-55 0.013913710260897529 56-57 0.002885806572630598 58-59 4.466129219547354E-4 60-61 0.0022674194499240413 62-63 0.0195135492054069 64-65 0.012436452134431864 66-67 0.0015459678067663918 68-69 0.014360323182852262 70-71 0.0017520968476685776 72-73 0.00886354875879398 74-75 0.0 76-77 0.0037103227362393406 78-79 9.619355242101995E-4 80-81 0.0018895162082700344 82-83 0.0012024194052627493 84-85 0.0 86-87 0.003229354974134241 88-89 0.011886774692026035 90-91 0.015528387747964646 92-93 0.002748387212029141 94-95 0.01886080724254998 96-97 0.011302742409469843 98-99 7.558064833080138E-4 100-101 0.005496774424058282 102-103 0.0 104-105 8.932258439094708E-4 106-107 3.435484015036427E-5 108-109 0.0 110-111 6.870968030072852E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.021574839614428756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1455399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.28633533244038 #Duplication Level Percentage of deduplicated Percentage of total 1 79.53383108463095 48.74337042126363 2 11.671798629683556 14.30643529503009 3 3.851990060749002 7.0822306368087204 4 1.6960056514102917 4.157678843121804 5 0.8919103077373111 2.7330957103224467 6 0.5413818854426969 1.9907587064489969 7 0.3463409047092961 1.485817537774829 8 0.2482834858018021 1.2173107974685153 9 0.166018006096773 0.9157171673582964 >10 0.955323421686731 10.602982016605898 >50 0.06563808702170192 2.8093690488519694 >100 0.03090822734126021 3.434223204950483 >500 1.1404953771585233E-4 0.04074982398157739 >1k 4.5619815086340933E-4 0.4802607900127243 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2477 0.17019387810490455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1885 0.12951774736687327 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1470 0.10100323004207094 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.7483872120291413E-4 0.0 0.0 0.0 0.0 8 2.7483872120291413E-4 0.0 0.0 0.0 0.0 9 2.7483872120291413E-4 0.0 0.0 0.0 0.0 10-11 3.4354840150364267E-4 0.0 0.0 6.870968030072853E-5 0.0 12-13 4.1225808180437115E-4 0.0 0.0 6.870968030072853E-5 0.0 14-15 6.183871227065568E-4 0.0 0.0 2.0612904090218557E-4 0.0 16-17 6.52741962856921E-4 0.0 0.0 2.0612904090218557E-4 6.870968030072853E-5 18-19 8.588710037591066E-4 0.0 0.0 2.4048388105254987E-4 6.870968030072853E-5 20-21 0.001030645204510928 0.0 0.0 5.496774424058283E-4 6.870968030072853E-5 22-23 0.001030645204510928 0.0 0.0 8.245161636087424E-4 6.870968030072853E-5 24-25 0.001305483925713842 0.0 0.0 9.962903643605636E-4 6.870968030072853E-5 26-27 0.001305483925713842 0.0 0.0 0.0014429032863152993 6.870968030072853E-5 28-29 0.0016146774870671205 0.0 0.0 0.002061290409021856 1.030645204510928E-4 30-31 0.0017177420075182132 0.0 0.0 0.004122580818043712 1.3741936060145707E-4 32-33 0.0017520968476685776 0.0 0.0 0.007180161591426131 2.0612904090218557E-4 34-35 0.002198709769623313 0.0 0.0 0.011955484372326766 2.0612904090218557E-4 36-37 0.0025766130112773195 0.0 0.0 0.02023500084856455 2.0612904090218557E-4 38-39 0.002954516252931327 0.0 0.0 0.03617564667833357 2.0612904090218557E-4 40-41 0.003023225933232055 0.0 0.0 0.05702903464960468 2.0612904090218557E-4 42-43 0.003366774334735698 0.0 0.0 0.0784321000632816 2.0612904090218557E-4 44-45 0.0038477420968407974 0.0 0.0 0.10158726232462713 2.0612904090218557E-4 46-47 0.004156935658194076 0.0 0.0 0.12999871512897837 2.0612904090218557E-4 48-49 0.004363064699096262 0.0 0.0 0.16387258751723754 3.4354840150364267E-4 50-51 0.004397419539246626 0.0 0.0 0.20434258921436665 3.4354840150364267E-4 52-53 0.004500484059697718 0.0 0.0 0.24367888118653375 3.779032416540069E-4 54-55 0.004534838899848083 0.0 0.0 0.28596968941163214 4.1225808180437115E-4 56-57 0.004603548580148812 0.0 0.0 0.3356811431092092 4.1225808180437115E-4 58-59 0.004637903420299176 0.0 0.0 0.3932255003610694 4.1225808180437115E-4 60-61 0.004740967940750268 0.0 0.0 0.464580503353376 4.1225808180437115E-4 62-63 0.004740967940750268 0.0 0.0 0.5336680868957584 4.1225808180437115E-4 64-65 0.004740967940750268 0.0 0.0 0.6061224447728768 4.1225808180437115E-4 66-67 0.004878387301351725 0.0 0.0 0.6858943836020226 4.1225808180437115E-4 68-69 0.005015806661953183 0.0 0.0 0.7795800326920659 4.1225808180437115E-4 70-71 0.005221935702855368 0.0 0.0 0.8887940695300739 4.1225808180437115E-4 72-73 0.005428064743757554 0.0 0.0 1.0056005260413123 4.809677621050997E-4 74-75 0.005531129264208647 0.0 0.0 1.12326585355631 4.809677621050997E-4 76-77 0.005565484104359011 0.0 0.0 1.2465653748559675 4.809677621050997E-4 78-79 0.005565484104359011 0.0 0.0 1.4017461878151627 4.809677621050997E-4 80-81 0.006424355108118117 0.0 0.0 1.5769215177418703 5.496774424058283E-4 82-83 0.0066648389891706674 0.0 0.0 1.762781202955341 6.870968030072853E-4 84-85 0.006836613189922489 0.0 0.0 1.9502212108157282 6.870968030072853E-4 86-87 0.007042742230824675 0.0 0.0 2.1424708962971666 6.870968030072853E-4 88-89 0.007077097070975038 0.0 0.0 2.3656055830737825 6.870968030072853E-4 90-91 0.007867258394433417 0.0 0.0 2.62567172301204 6.870968030072853E-4 92-93 0.008039032595185238 0.0 0.0 2.906625605761719 6.870968030072853E-4 94-95 0.008039032595185238 0.0 0.0 3.191186746727186 6.870968030072853E-4 96-97 0.008039032595185238 0.0 0.0 3.4703885326291966 6.870968030072853E-4 98-99 0.008485645517139973 0.0 0.0 3.787964674979164 6.870968030072853E-4 100-101 0.008657419717891794 0.0 0.0 4.149240174000394 6.870968030072853E-4 102-103 0.008897903598944344 0.0 0.0 4.544458255090185 6.870968030072853E-4 104-105 0.010409516565560372 0.0 0.0 4.948849078500123 6.870968030072853E-4 106-107 0.010615645606462557 0.0 0.0 5.348052321047355 6.870968030072853E-4 108-109 0.010718710126913649 0.0 0.0 5.764295564309169 6.870968030072853E-4 110-111 0.010993548848116565 0.0 0.0 6.238461068064496 7.214516431576497E-4 112-113 0.010993548848116565 0.0 0.0 6.756325928491087 7.558064833080139E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5510 0.0 51.07389 1 GGTATCA 3115 0.0 44.88479 1 ATCAACG 6550 0.0 41.783604 3 TCAACGC 6555 0.0 41.75173 4 CAACGCA 6715 0.0 40.756905 5 TATCAAC 6740 0.0 40.252636 2 AACGCAG 6955 0.0 39.259544 6 GTACATG 9095 0.0 38.85734 1 TACATGG 9440 0.0 36.491856 2 ACATGGG 9535 0.0 34.568333 3 ACGCAGA 8035 0.0 33.464336 7 CGCAGAG 8005 0.0 32.925453 8 CATGGGG 3530 0.0 31.517828 4 GCAGAGT 8440 0.0 29.889097 9 CATGGGA 5730 0.0 29.800026 4 ATGGGAT 2340 0.0 26.442764 5 GAGTACT 3350 0.0 25.491825 12-13 AGTACAT 6380 0.0 24.24611 2 AGAGTAC 7955 0.0 23.263988 10-11 CGTAACG 90 2.1807642E-5 23.13583 100-101 >>END_MODULE