FastQCFastQC Report
Thu 26 May 2016
SRR1364562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455399
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57350.3940500165246781No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51100.35110646633672277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34060.2340251711042814No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.10265226236928843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48550.055.3677521
GTATCAA80200.045.928651
TCAACGC102600.036.039654
ATCAACG103400.035.7608153
CAACGCA104000.035.3547825
TATCAAC104250.035.1836552
AACGCAG106800.034.4835856
GTACATG87850.033.855431
TACATGG90150.032.430382
ACATGGG90050.030.945573
ACGCAGA123000.029.699997
CGCAGAG124450.029.4017528
GCAGAGT129950.027.1958839
CATGGGA56400.026.8158114
GAGTACT76800.026.61083612-13
AGTACTT80250.025.4668212-13
GTACTTT79850.025.40811714-15
TATAGCG1200.001288441424.7902985
ATGGGAT23150.024.1584955
AGAGTAC124200.024.0717410-11
Produced by FastQC (version 0.11.5)