##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364562_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1455399 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.805433424098823 31.0 30.0 34.0 26.0 34.0 2 29.711873513723727 31.0 30.0 34.0 25.0 34.0 3 29.984347247730692 31.0 30.0 34.0 26.0 34.0 4 33.52132439282973 35.0 33.0 37.0 28.0 37.0 5 32.96159747258312 35.0 33.0 37.0 26.0 37.0 6 32.99199463514816 35.0 33.0 37.0 26.0 37.0 7 32.772666464660205 35.0 33.0 37.0 25.0 37.0 8 32.69047800637488 35.0 33.0 37.0 25.0 37.0 9 33.87500884637134 38.0 33.0 39.0 23.0 39.0 10-11 33.81407572768705 38.0 33.0 39.0 23.0 39.0 12-13 33.701810637495285 38.0 33.0 39.0 22.5 39.0 14-15 34.607586648060085 38.5 33.0 41.0 22.0 41.0 16-17 34.623559930987994 38.5 33.0 41.0 22.0 41.0 18-19 34.6585857211665 39.0 33.5 41.0 22.0 41.0 20-21 34.682551657655395 39.0 34.0 41.0 22.5 41.0 22-23 34.64598264805734 39.0 34.0 41.0 23.0 41.0 24-25 34.6018775607239 39.0 33.5 41.0 23.0 41.0 26-27 34.468941850310465 39.0 33.0 41.0 22.0 41.0 28-29 34.29970887708457 38.5 33.0 41.0 21.5 41.0 30-31 34.11295424828518 38.0 33.0 40.0 20.0 41.0 32-33 33.86392494429363 38.0 33.0 40.0 18.5 41.0 34-35 33.66585932792313 38.0 33.0 40.0 17.5 41.0 36-37 33.43654523604867 38.0 32.0 40.0 16.0 41.0 38-39 33.24835045235018 38.0 32.0 40.0 15.0 41.0 40-41 33.25421035743463 38.0 32.0 40.0 14.5 41.0 42-43 33.093306715203184 38.0 32.0 40.0 9.5 41.0 44-45 32.88169223697419 38.0 32.0 40.0 8.5 41.0 46-47 32.57837885006104 38.0 31.0 40.0 4.0 41.0 48-49 32.23783580997376 38.0 31.0 40.0 2.0 41.0 50-51 32.04364576312063 38.0 30.0 40.0 2.0 41.0 52-53 31.85573234556297 37.0 30.0 40.0 2.0 41.0 54-55 31.65759458402816 37.0 30.0 40.0 2.0 41.0 56-57 31.44257657178547 37.0 30.0 40.0 2.0 41.0 58-59 31.227766062777285 36.5 30.0 40.0 2.0 41.0 60-61 30.990347664111354 36.0 29.0 40.0 2.0 41.0 62-63 30.813040272804912 36.0 29.0 39.5 2.0 41.0 64-65 30.673839613741663 35.5 29.0 39.0 2.0 41.0 66-67 30.373463222112974 35.0 29.0 39.0 2.0 41.0 68-69 30.08462352935518 35.0 29.0 39.0 2.0 40.5 70-71 29.77425331472675 35.0 29.0 38.0 2.0 40.0 72-73 29.486685094602926 35.0 29.0 37.0 2.0 40.0 74-75 29.140635660736336 35.0 28.0 37.0 2.0 39.0 76-77 28.831488478417256 34.0 28.0 36.0 2.0 39.0 78-79 28.55667346205405 34.0 28.0 36.0 2.0 38.5 80-81 28.28581818456657 34.0 27.5 35.5 2.0 37.0 82-83 28.00239144042287 34.0 27.0 35.0 2.0 37.0 84-85 27.779705427858616 34.0 27.0 35.0 2.0 36.5 86-87 27.567473249603715 34.0 26.0 35.0 2.0 36.0 88-89 27.38611301780474 34.0 26.0 35.0 2.0 36.0 90-91 27.244979211886225 34.0 26.0 35.0 2.0 35.5 92-93 27.134963333079106 34.0 26.0 35.0 2.0 35.0 94-95 27.01896112337579 34.0 26.0 35.0 2.0 35.0 96-97 26.898453963483554 34.0 25.0 35.0 2.0 35.0 98-99 26.806756772541412 34.0 25.0 35.0 2.0 35.0 100-101 26.704636323097652 34.0 25.0 35.0 2.0 35.0 102-103 25.447488283281764 31.5 23.5 34.0 2.0 35.0 104-105 26.36871263481698 33.0 25.0 35.0 2.0 35.0 106-107 26.529635859307312 34.0 25.0 35.0 2.0 35.0 108-109 26.53557134504009 34.0 25.0 35.0 2.0 35.0 110-111 26.447711246194345 34.0 25.0 35.0 2.0 35.0 112-113 26.358998460216064 34.0 25.0 35.0 2.0 35.0 114-115 26.254083588074472 34.0 24.0 35.0 2.0 35.0 116-117 26.123074840645074 33.5 24.0 35.0 2.0 35.0 118-119 25.99140235770397 33.0 24.0 35.0 2.0 35.0 120-121 25.787559287865392 33.0 23.0 35.0 2.0 35.0 122-123 25.490563068959098 33.0 19.5 35.0 2.0 35.0 124-125 23.728474803129586 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.996534138247199 1101 2 1.1203825458985044 1101 3 0.9550525525387705 1101 4 0.8226820957083518 1101 5 0.9070716356245256 1101 6 1.037543855917022 1101 7 1.116930031995615 1101 8 1.1955318507079298 1101 9 1.3313557200085953 1101 10-11 1.2988087815589253 1101 12-13 1.117474812578692 1101 14-15 1.3958247815441496 1101 16-17 1.336505581445806 1101 18-19 1.4841150097387086 1101 20-21 1.2771119790281418 1101 22-23 1.3808561023070922 1101 24-25 1.4224052430235332 1101 26-27 1.3766099492750001 1101 28-29 1.4630767138826926 1101 30-31 1.572486258414358 1101 32-33 1.5828858907625118 1101 34-35 1.6282666490523212 1101 36-37 1.7738908746006246 1101 38-39 1.697606102913042 1101 40-41 1.6302638274794674 1101 42-43 1.5684670216266383 1101 44-45 1.601684267290402 1101 46-47 1.6341201813924826 1101 48-49 1.7727662153130765 1101 50-51 1.8555954787996995 1101 52-53 1.8055163734836945 1101 54-55 1.9295517595484064 1101 56-57 1.879804706961039 1101 58-59 1.7821412132385959 1101 60-61 1.7443336691894586 1101 62-63 1.7006894341821024 1101 64-65 1.6048693259645965 1101 66-67 1.7243653732615378 1101 68-69 1.751079022291215 1101 70-71 1.687464435761985 1101 72-73 1.5842462626362561 1101 74-75 1.6262641997963136 1101 76-77 1.5899686876923411 1101 78-79 1.5061180971617816 1101 80-81 1.4469692063672888 1101 82-83 1.5516416301929254 1101 84-85 1.5784839568423088 1101 86-87 1.5294693531143864 1101 88-89 1.534327541399552 1101 90-91 1.543066841193987 1101 92-93 1.5596188371212634 1101 94-95 1.5134025140035732 1101 96-97 1.5178577582736708 1101 98-99 1.4917701263092802 1101 100-101 1.4562576802729659 1101 102-103 1.3793927944471456 1101 104-105 1.454313627225396 1101 106-107 1.3199756913084286 1101 108-109 1.3739586895097915 1101 110-111 1.3483203757189344 1101 112-113 1.463702499246338 1101 114-115 1.5129604800270045 1101 116-117 1.5366576189523045 1101 118-119 1.573924566595256 1101 120-121 1.4194258794390748 1101 122-123 1.5464303776291537 1101 124-125 1.8262597963087472 1102 1 0.48918536639346755 1102 2 -0.07311346344503278 1102 3 0.38234308543203355 1102 4 0.5009157925268966 1102 5 0.6287004740757922 1102 6 0.7147903368425261 1102 7 0.7179095670327413 1102 8 0.6175446190013574 1102 9 1.115321481463802 1102 10-11 0.998885759548763 1102 12-13 0.9215271417982507 1102 14-15 1.1763361345269132 1102 16-17 1.2987428792303248 1102 18-19 1.3954259737087185 1102 20-21 1.1761714130394267 1102 22-23 1.3198754716184595 1102 24-25 1.260379281730529 1102 26-27 1.1779917691408528 1102 28-29 0.8573822063723924 1102 30-31 1.234238871599679 1102 32-33 1.5082759765847698 1102 34-35 1.4727411768491194 1102 36-37 1.330166698654807 1102 38-39 0.6885090891846417 1102 40-41 0.39371125540931473 1102 42-43 0.7500701292105774 1102 44-45 0.544564396238421 1102 46-47 1.0113411347674273 1102 48-49 0.5193824029428313 1102 50-51 0.5399252902346845 1102 52-53 0.2148489522858803 1102 54-55 0.41084040385474907 1102 56-57 0.34521657026891717 1102 58-59 0.4686261824812874 1102 60-61 0.24350543571854644 1102 62-63 0.38493368314123444 1102 64-65 0.38574071906854357 1102 66-67 0.2555795810377255 1102 68-69 0.4498621149200801 1102 70-71 0.11265995872661705 1102 72-73 0.23774929770387843 1102 74-75 0.3795237630267003 1102 76-77 -0.05064575819037742 1102 78-79 0.16262400803222476 1102 80-81 0.12253013475686103 1102 82-83 -0.1142846245539566 1102 84-85 0.031191627984203052 1102 86-87 0.23855524089707814 1102 88-89 -0.01544186349225285 1102 90-91 0.13764782791288255 1102 92-93 0.3406565546419742 1102 94-95 0.4497098634531511 1102 96-97 0.1355064449602601 1102 98-99 0.0037084919586867215 1102 100-101 0.20466700889838663 1102 102-103 0.18128149432673624 1102 104-105 0.3763450328898017 1102 106-107 0.4664645800467113 1102 108-109 0.47778720084696147 1102 110-111 0.5688603398900014 1102 112-113 0.741828268743852 1102 114-115 0.5743844542374035 1102 116-117 0.5330809380308743 1102 118-119 0.7670294735326486 1102 120-121 0.9456749221559271 1102 122-123 0.897673659704072 1102 124-125 1.153212936310421 1103 1 0.5081834274217663 1103 2 0.0834344135200844 1103 3 0.5542359651485889 1103 4 0.586990008027044 1103 5 0.6777366008875347 1103 6 0.721402868282432 1103 7 0.7370160953836944 1103 8 0.7857895514667206 1103 9 1.1737193036368296 1103 10-11 1.1786742274468835 1103 12-13 1.1460539318072165 1103 14-15 1.2174283077104278 1103 16-17 1.0489280592624297 1103 18-19 1.212916736476302 1103 20-21 1.2749940409508937 1103 22-23 1.2766203880882045 1103 24-25 1.3012477055678247 1103 26-27 1.3651115301265762 1103 28-29 1.4564367234117057 1103 30-31 1.4180818972360072 1103 32-33 1.493696965144217 1103 34-35 1.4287077976869256 1103 36-37 1.4291889405134697 1103 38-39 1.583402171259955 1103 40-41 1.5394025566518508 1103 42-43 1.6477742754071159 1103 44-45 1.643635760732728 1103 46-47 1.6470752115089127 1103 48-49 1.769015876147904 1103 50-51 1.758789820984795 1103 52-53 1.6857219741533065 1103 54-55 1.7068548260334175 1103 56-57 1.7451471360386392 1103 58-59 1.8834234915073367 1103 60-61 1.697764311440558 1103 62-63 1.6788782345701705 1103 64-65 1.7246111861803222 1103 66-67 1.7037896525220475 1103 68-69 1.745486395472227 1103 70-71 1.5741043686558598 1103 72-73 1.684956188426554 1103 74-75 1.7046031684894807 1103 76-77 1.6981987240882752 1103 78-79 1.525810369089843 1103 80-81 1.5314243037769621 1103 82-83 1.6830119078801395 1103 84-85 1.6693783345093003 1103 86-87 1.7827098649461206 1103 88-89 1.7441989033357537 1103 90-91 1.6660888874701527 1103 92-93 1.5640610012721687 1103 94-95 1.5731115084840859 1103 96-97 1.6157998443356192 1103 98-99 1.6577432586697043 1103 100-101 1.493933173510893 1103 102-103 1.3338019777086174 1103 104-105 1.5088438303790355 1103 106-107 1.5185748941533141 1103 108-109 1.523058489357357 1103 110-111 1.5000313875575095 1103 112-113 1.4364776116836389 1103 114-115 1.5541022953325836 1103 116-117 1.5723617258557283 1103 118-119 1.457657575049737 1103 120-121 1.5291891763332899 1103 122-123 1.459670306492633 1103 124-125 1.7213476409887285 1104 1 0.4866950642676393 1104 2 0.5851974635348753 1104 3 0.39692036783296913 1104 4 0.12100439193836365 1104 5 0.4124004815086906 1104 6 0.5189268857567129 1104 7 0.6462433113286465 1104 8 0.5805988029654259 1104 9 0.6639611953514688 1104 10-11 0.7501913380239813 1104 12-13 0.8125991250287328 1104 14-15 0.8558206208302579 1104 16-17 0.7921617676692492 1104 18-19 0.7081735458935015 1104 20-21 0.6376417528689657 1104 22-23 0.7658195705984596 1104 24-25 0.8241054409866706 1104 26-27 0.7316931564344173 1104 28-29 0.8411207263342249 1104 30-31 0.7939859021376634 1104 32-33 0.8684067843799284 1104 34-35 1.198591789801199 1104 36-37 1.24671134033008 1104 38-39 1.3013054900886516 1104 40-41 1.1265258584915543 1104 42-43 1.2007971210637791 1104 44-45 1.0995269424763947 1104 46-47 0.961231129729164 1104 48-49 0.8885916454255423 1104 50-51 0.9757427659359408 1104 52-53 0.9717497066034717 1104 54-55 0.6580164169710976 1104 56-57 0.8032712400862323 1104 58-59 0.8788536086067822 1104 60-61 1.0518576742663157 1104 62-63 1.0758590304326887 1104 64-65 1.0430211503789124 1104 66-67 1.0856920537236938 1104 68-69 0.8077253267301892 1104 70-71 0.3117848050369112 1104 72-73 0.3119412413771059 1104 74-75 0.9499180006387071 1104 76-77 1.0746347307858066 1104 78-79 1.1978317987592249 1104 80-81 1.1183810978228372 1104 82-83 1.028355335349577 1104 84-85 1.046640169759815 1104 86-87 1.0989399280759287 1104 88-89 1.0955780370192336 1104 90-91 1.1683403663116714 1104 92-93 1.0802568144377176 1104 94-95 1.0906528943049238 1104 96-97 1.0732797842174442 1104 98-99 1.1648021691450587 1104 100-101 1.0647662689972535 1104 102-103 1.0513254869807085 1104 104-105 1.1281880455618634 1104 106-107 1.1925433287134268 1104 108-109 1.1688540915116334 1104 110-111 1.0539692655664368 1104 112-113 1.0617276194229888 1104 114-115 1.0160213097352866 1104 116-117 0.9735779574756762 1104 118-119 0.939445684442763 1104 120-121 1.0069086261793743 1104 122-123 1.062826224176046 1104 124-125 1.428341612529767 1105 1 0.4061446529497168 1105 2 0.1672075276060987 1105 3 0.410038233016202 1105 4 0.46364650554966147 1105 5 0.12272478877767412 1105 6 0.5962753348578929 1105 7 0.5800508304590508 1105 8 0.6932974621315324 1105 9 0.9464613872240335 1105 10-11 0.7417254053527742 1105 12-13 0.8657934834068826 1105 14-15 0.9616102688080659 1105 16-17 1.0185117865722546 1105 18-19 1.0585318679039801 1105 20-21 0.9170845975857915 1105 22-23 0.860658358560805 1105 24-25 0.8051159200711382 1105 26-27 0.9849009814238983 1105 28-29 1.0189317837368108 1105 30-31 1.0649667226984363 1105 32-33 1.229917876745958 1105 34-35 1.2185457368330006 1105 36-37 1.2295521592990326 1105 38-39 1.294782355942722 1105 40-41 1.1386272432429436 1105 42-43 1.1340106347194236 1105 44-45 1.1043963305592612 1105 46-47 1.0577061642662215 1105 48-49 1.0803697817602327 1105 50-51 0.9030181791015224 1105 52-53 1.0095849328425395 1105 54-55 1.034500497944169 1105 56-57 1.0424320392425628 1105 58-59 0.7469944309867991 1105 60-61 0.8103724633770497 1105 62-63 1.0922601371820555 1105 64-65 0.8822032509428759 1105 66-67 1.1331327224635324 1105 68-69 1.084469519387298 1105 70-71 1.1103801735175054 1105 72-73 1.0124857330711592 1105 74-75 0.8134815964442303 1105 76-77 0.8541710245469645 1105 78-79 0.7692357516182682 1105 80-81 0.826981039266176 1105 82-83 0.7670325445844774 1105 84-85 0.7589585304006405 1105 86-87 1.0188640460306537 1105 88-89 0.9184676697893615 1105 90-91 0.7859560263817151 1105 92-93 0.8041525523085298 1105 94-95 0.7039396961730517 1105 96-97 0.7901080383761077 1105 98-99 0.7327618318473021 1105 100-101 0.8598342025439685 1105 102-103 0.5848318550178675 1105 104-105 0.6734923554460011 1105 106-107 0.631610732123356 1105 108-109 0.6629193665573965 1105 110-111 0.5914274977521359 1105 112-113 0.542916468926677 1105 114-115 0.5428657025083723 1105 116-117 0.2721773233013245 1105 118-119 0.4810638203794966 1105 120-121 0.5309216550370053 1105 122-123 0.6467874367822155 1105 124-125 1.0161017242138115 1106 1 0.22017385920375432 1106 2 0.04341676798982874 1106 3 0.36587573647529226 1106 4 0.18444889924028018 1106 5 0.4436188483662775 1106 6 0.6414769998334506 1106 7 0.7409273639891722 1106 8 0.865947874916337 1106 9 0.9598322132548418 1106 10-11 0.8621053576173239 1106 12-13 0.9088173626615657 1106 14-15 0.9858844363202834 1106 16-17 0.9647250913873009 1106 18-19 0.9224274667878518 1106 20-21 0.92631172146735 1106 22-23 0.8578788352234739 1106 24-25 0.887510014250644 1106 26-27 0.9836211179426684 1106 28-29 0.9936552006263923 1106 30-31 0.9946412511091012 1106 32-33 1.0193796134399804 1106 34-35 1.0374371271567782 1106 36-37 1.0379567402878678 1106 38-39 0.9757345859030551 1106 40-41 0.9301687580939699 1106 42-43 0.6891852899634046 1106 44-45 0.830945858969919 1106 46-47 0.8023121450336816 1106 48-49 0.9566287465697556 1106 50-51 0.95500470594407 1106 52-53 0.7817656299645783 1106 54-55 0.9141536952968075 1106 56-57 0.4625659636960293 1106 58-59 0.8069440723944155 1106 60-61 0.8806820808016411 1106 62-63 0.7127640401891568 1106 64-65 1.019914687849063 1106 66-67 0.8217180121047072 1106 68-69 0.9616833373007729 1106 70-71 0.9142893781572994 1106 72-73 0.9908779478114944 1106 74-75 0.7861814453972791 1106 76-77 0.715328897436919 1106 78-79 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50643.0 3 16583.0 4 7834.0 5 6477.0 6 7152.0 7 8473.0 8 9565.0 9 10225.0 10 11317.0 11 12619.0 12 14000.0 13 16588.0 14 19676.0 15 16882.0 16 10701.0 17 8911.0 18 8273.0 19 7880.0 20 7826.0 21 7867.0 22 9012.0 23 11186.0 24 14788.0 25 19483.0 26 24070.0 27 26097.0 28 29311.0 29 35201.0 30 40409.0 31 47132.0 32 57775.0 33 71119.0 34 91091.0 35 124972.0 36 198615.0 37 292104.0 38 103218.0 39 324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48473536018223 19.93907899098633 14.588649077656749 22.987536571174694 2 15.698169367987747 21.2297108902782 39.066881315707924 24.005238426026125 3 19.466964042163006 26.286468521690615 29.34727865004717 24.89928878609921 4 12.857771664374141 17.576409903713895 36.626478679504814 32.93933975240716 5 12.78611569748227 39.431867137465396 33.79691754632235 13.985099618729985 6 30.911248393052354 37.72642416272101 17.06988942551149 14.292438018715142 7 27.543054166071173 33.55009289695584 20.815102076714194 18.09175086025879 8 26.336808096466076 35.608660728580254 19.19880496182708 18.85572621312659 9 26.77910084720724 16.03021911953661 19.793317163333036 37.397362869923114 10-11 24.995422197104904 27.42957655752658 26.669334904501707 20.90566634086681 12-13 25.94374463635058 24.848134429115316 27.612496641814378 21.595624292719727 14-15 23.025316786198687 25.75192490301622 26.11732323391952 25.10543507686557 16-17 21.72440412245331 28.92574561347757 26.755957401269963 22.59389286279915 18-19 21.637809428858247 26.90735016143517 30.04453123335919 21.410309176347393 20-21 22.62714315848637 25.85879219753921 30.258274946801087 21.25578969717333 22-23 22.141453993028716 25.948691733332236 30.093568842633534 21.816285431005518 24-25 21.81137268886402 26.215250938058908 30.273210301779784 21.700166071297286 26-27 21.6777323606791 26.255102552633332 30.261014333526408 21.80615075316116 28-29 21.657531714670686 26.173234968555015 30.448419986546643 21.720813330227656 30-31 21.81817494721379 26.076251254810533 30.358856918274647 21.74671687970103 32-33 21.67666736063444 26.085561416491288 30.232328042000855 22.005443180873424 34-35 22.010287213334625 26.113629320894134 30.226487719175292 21.64959574659595 36-37 22.041481408191153 25.948176410729978 30.0951835201206 21.915158660958266 38-39 21.9277325324533 26.30718449030128 29.987240612368154 21.777842364877262 40-41 22.061029312236712 26.098066578306018 30.034306743374156 21.806597366083118 42-43 21.948826404305624 26.22648497078808 29.703126084324644 22.121562540581653 44-45 22.106618185116247 26.31903691015316 29.48146865567449 22.0928762490561 46-47 22.278323676187767 26.555879178149773 28.83023830578419 22.335558839878274 48-49 22.36166851839255 26.770012896806993 28.43402393433004 22.43429465047042 50-51 22.420438402566997 26.93466905089503 28.21359800329463 22.43129454324334 52-53 22.51554384742603 27.091539845774253 28.088860855339327 22.30405545146039 54-55 22.387915616267428 27.005584035718037 28.135171179862017 22.47132916815251 56-57 22.357479869678997 27.106026504650664 27.998848370764147 22.53764525490619 58-59 22.3742546249583 26.90963221923448 28.149195594450017 22.5669175613572 60-61 22.38915239051284 26.979371292683314 28.272762314664224 22.35871400213962 62-63 22.374139325367132 26.856518384305613 28.423648772604626 22.34569351772263 64-65 22.224737768604243 26.812739368171094 28.30133970594904 22.661183157275623 66-67 22.239502706817856 27.032552585236076 28.111225856277212 22.616718851668853 68-69 22.408192670254916 26.798639685282073 28.162939566036382 22.630228078426633 70-71 22.265440611131382 26.828759673464113 28.235659087301833 22.670140628102672 72-73 22.363008357158414 27.00451903567338 27.916640041665552 22.715832565502655 74-75 22.28012633165805 27.092801823309514 27.8833194594797 22.74375238555273 76-77 22.440547231377785 26.976691615151584 28.01266181988582 22.57009933358481 78-79 22.309243032323096 26.88802177272349 27.94556681707216 22.857168377881255 80-81 22.188275776300213 27.04056006711571 27.874009634484608 22.89715452209947 82-83 22.31267851633813 27.170109365198137 27.786847455577472 22.73036466288626 84-85 22.260356094789127 27.0726790385317 27.707900032911937 22.959064833767233 86-87 22.184500607737125 27.100609523573947 27.86297778135068 22.85191208733825 88-89 22.338968726488183 26.91439035031967 27.74867131377608 22.997969609416067 90-91 22.204014156942527 27.00816064872932 27.93471068758464 22.85311450674351 92-93 22.26709450893762 27.043731297646982 27.87929488478835 22.809879308627046 94-95 22.026880601127253 26.999331454810676 27.844563587030084 23.129224357031987 96-97 22.276139196495077 26.768609601710686 27.778728310671713 23.176522891122527 98-99 22.043382199604018 26.755803435079756 27.730506370732044 23.470307994584182 100-101 22.048541207737898 26.811036552602687 27.812010867249974 23.328411372409445 102-103 22.105003507629178 26.638949181633354 27.92388891293728 23.33215839780019 104-105 22.002763503341697 26.6335898265699 27.87191003978978 23.491736630298632 106-107 21.924333409029927 26.843421078398194 27.76449174660903 23.467753765962847 108-109 21.921865386009397 26.85614970745126 27.49398188880915 23.728003017730195 110-111 21.859816208160456 26.764218548003925 27.582792730365917 23.793172513469695 112-113 21.9466964042163 26.693848216193633 27.69487954849495 23.664575831095117 114-115 21.85610269073979 26.876650320633722 27.57456889828837 23.692678090338113 116-117 21.57322493694169 26.8228162861181 27.68165293503706 23.92230584190315 118-119 21.566010420510114 26.709342249101446 27.598514221873177 24.126133108515262 120-121 21.440740474807278 26.85330844196111 27.67583682867244 24.03011425455917 122-123 21.457278317930395 26.860102380870583 27.606211564228538 24.076407736970488 124-125 21.415747195515575 26.689773207048447 27.554532214610962 24.339947382825017 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 479.0 1 348.0 2 449.0 3 679.0 4 461.5 5 282.5 6 571.0 7 1408.0 8 2255.5 9 2427.0 10 1844.5 11 1198.0 12 985.5 13 997.5 14 1123.0 15 1287.0 16 1457.0 17 1642.0 18 1922.0 19 2216.0 20 2593.0 21 3099.0 22 3736.0 23 4527.0 24 5439.5 25 6693.0 26 8631.5 27 10869.5 28 12700.0 29 14631.5 30 17550.5 31 21093.5 32 25076.5 33 28930.5 34 32588.5 35 35746.0 36 39405.5 37 43124.0 38 44144.5 39 44694.5 40 44916.5 41 44838.0 42 45013.0 43 45798.5 44 46805.0 45 47762.0 46 48420.5 47 47836.5 48 49134.5 49 49863.5 50 49193.0 51 48302.5 52 47108.0 53 46956.0 54 46261.5 55 44311.5 56 41318.5 57 38228.5 58 36228.0 59 32787.5 60 27768.0 61 22837.5 62 18234.5 63 14277.0 64 10468.5 65 7538.0 66 5729.0 67 4483.0 68 3408.5 69 2602.0 70 1960.5 71 1474.5 72 1159.5 73 964.0 74 722.0 75 490.5 76 374.0 77 254.0 78 170.5 79 110.5 80 73.0 81 54.5 82 33.5 83 19.0 84 12.0 85 8.0 86 7.0 87 5.0 88 3.0 89 1.5 90 1.0 91 1.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09516290721650901 2 0.0 3 0.0 4 0.09612484274071921 5 0.0 6 0.0 7 0.0015803226469167562 8 0.0032980646544349696 9 0.001992580728721127 10-11 4.466129219547354E-4 12-13 0.0 14-15 6.870968030072853E-5 16-17 2.4048388105254987E-4 18-19 0.0010993548848116565 20-21 8.245161636087424E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.030645204510928E-4 52-53 0.0 54-55 0.0 56-57 0.004775322780900633 58-59 5.15322602255464E-4 60-61 0.0 62-63 0.0 64-65 3.435484015036426E-4 66-67 0.0 68-69 3.435484015036427E-5 70-71 0.0 72-73 0.0 74-75 0.0016146774870671205 76-77 0.0 78-79 0.0 80-81 1.3741936060145707E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.619355242101995E-4 90-91 0.0 92-93 0.00233612913022477 94-95 0.0 96-97 0.005153226022554639 98-99 0.002233064609773677 100-101 0.001168064565112385 102-103 0.0 104-105 0.0 106-107 0.0018895162082700344 108-109 3.435484015036427E-5 110-111 1.717742007518213E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 5.840322825561925E-4 122-123 0.003366774334735698 124-125 0.07702355161711667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1455399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.798836273119214 #Duplication Level Percentage of deduplicated Percentage of total 1 79.28607492458481 47.4121501315351 2 11.678495505235944 13.967208812679255 3 3.9673077296114725 7.117211561043503 4 1.732191729497911 4.143321985035872 5 0.9074203627597366 2.713134085178196 6 0.5492731655648749 1.9707537658099117 7 0.35319619885067166 1.478450516715156 8 0.2505727257777155 1.1987165922632648 9 0.18673511296348086 1.0049888201891053 >10 0.9781545193101374 10.749562855817995 >50 0.07367644187915853 3.023347126419417 >100 0.03562915208894765 3.801038676087961 >500 8.09729375036112E-4 0.33099126233869186 >1k 2.313512500103177E-4 0.3389461202632372 >5k 2.313512500103177E-4 0.7501776886234302 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5735 0.3940500165246781 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5110 0.35110646633672277 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3406 0.2340251711042814 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1494 0.10265226236928843 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.870968030072853E-5 0.0 0.0 0.0 0.0 3 6.870968030072853E-5 0.0 0.0 0.0 0.0 4 6.870968030072853E-5 0.0 0.0 0.0 0.0 5 6.870968030072853E-5 0.0 0.0 0.0 0.0 6 1.3741936060145707E-4 0.0 0.0 0.0 0.0 7 4.809677621050997E-4 0.0 0.0 0.0 0.0 8 4.809677621050997E-4 0.0 0.0 0.0 0.0 9 4.809677621050997E-4 0.0 0.0 0.0 0.0 10-11 5.15322602255464E-4 0.0 0.0 3.435484015036427E-5 0.0 12-13 6.52741962856921E-4 0.0 0.0 6.870968030072853E-5 0.0 14-15 6.870968030072853E-4 0.0 0.0 1.7177420075182133E-4 1.030645204510928E-4 16-17 7.901613234583781E-4 0.0 0.0 2.0612904090218557E-4 2.0612904090218557E-4 18-19 9.962903643605636E-4 0.0 0.0 2.4048388105254987E-4 2.0612904090218557E-4 20-21 0.001030645204510928 0.0 0.0 5.15322602255464E-4 2.0612904090218557E-4 22-23 0.001168064565112385 0.0 0.0 7.558064833080138E-4 2.4048388105254987E-4 24-25 0.001545967806766392 0.0 0.0 9.275806840598351E-4 3.091935613532784E-4 26-27 0.0015803226469167562 0.0 0.0 0.001305483925713842 3.4354840150364267E-4 28-29 0.001717742007518213 0.0 0.0 0.0020269355688714917 3.4354840150364267E-4 30-31 0.0017864516878189417 0.0 0.0 0.0038133872566904333 3.4354840150364267E-4 32-33 0.001820806527969306 0.0 0.0 0.006802258349772125 3.4354840150364267E-4 34-35 0.0019925807287211276 0.0 0.0 0.011577581130672757 3.4354840150364267E-4 36-37 0.00233612913022477 0.0 0.0 0.02006322664781273 3.4354840150364267E-4 38-39 0.002610967851427684 0.0 0.0 0.03607258215788248 3.4354840150364267E-4 40-41 0.0026453226915780486 0.0 0.0 0.05726951853065723 3.4354840150364267E-4 42-43 0.002954516252931327 0.0 0.0 0.07832903554283052 3.4354840150364267E-4 44-45 0.0031606452938335123 0.0 0.0 0.10096887520192058 3.779032416540069E-4 46-47 0.0034011291748860624 0.0 0.0 0.12893371508431709 4.1225808180437115E-4 48-49 0.0037103227362393406 0.0 0.0 0.16181129710821568 4.809677621050997E-4 50-51 0.0037103227362393406 0.0 0.0 0.20042613743722512 4.809677621050997E-4 52-53 0.003744677576389705 0.0 0.0 0.23866307452458052 5.496774424058283E-4 54-55 0.0038133872566904333 0.0 0.0 0.27968275366411544 6.183871227065568E-4 56-57 0.004260000178645169 0.0 0.0 0.32798565891552767 6.183871227065568E-4 58-59 0.004260000178645169 0.0 0.0 0.38364049995911775 6.183871227065568E-4 60-61 0.004397419539246626 0.0 0.0 0.45207534153864337 6.183871227065568E-4 62-63 0.004466129219547354 0.0 0.0 0.5189298604712522 6.870968030072853E-4 64-65 0.004534838899848083 3.435484015036427E-5 0.0 0.5905253473446114 6.870968030072853E-4 66-67 0.004878387301351725 6.870968030072853E-5 0.0 0.6665526085973674 6.870968030072853E-4 68-69 0.005015806661953183 6.870968030072853E-5 0.0 0.757627289835983 6.870968030072853E-4 70-71 0.005153226022554639 6.870968030072853E-5 0.0 0.8642990685028642 6.870968030072853E-4 72-73 0.0053593550634568245 1.030645204510928E-4 0.0 0.9777387506793669 8.245161636087423E-4 74-75 0.005462419583907918 1.3741936060145707E-4 0.0 1.0924152071012827 8.245161636087423E-4 76-77 0.005496774424058283 1.3741936060145707E-4 0.0 1.2112829540215433 8.588710037591065E-4 78-79 0.0055998389445093754 1.3741936060145707E-4 0.0 1.3625129603634467 9.275806840598351E-4 80-81 0.006905322870223217 1.3741936060145707E-4 0.0 1.5320540965054943 9.619355242101994E-4 82-83 0.00700838739067431 1.3741936060145707E-4 0.0 1.712348297614606 9.619355242101994E-4 84-85 0.007214516431576496 1.3741936060145707E-4 0.0 1.8928829826047702 9.619355242101994E-4 86-87 0.007420645472478681 1.3741936060145707E-4 0.0 2.0803229904651577 9.619355242101994E-4 88-89 0.007420645472478681 1.3741936060145707E-4 0.0 2.296758483412453 9.619355242101994E-4 90-91 0.007832903554283052 1.3741936060145707E-4 0.0 2.5511217198857494 9.619355242101994E-4 92-93 0.007901613234583781 1.3741936060145707E-4 0.0 2.8239678603599425 9.619355242101994E-4 94-95 0.007901613234583781 1.3741936060145707E-4 0.0 3.100593033250676 9.619355242101994E-4 96-97 0.007935968074734144 1.3741936060145707E-4 0.0 3.3736453027657705 9.619355242101994E-4 98-99 0.00821080679593706 1.3741936060145707E-4 0.0 3.6851406384091234 9.619355242101994E-4 100-101 0.008485645517139973 1.3741936060145707E-4 0.0 4.037449524151109 9.619355242101994E-4 102-103 0.008691774558042159 1.3741936060145707E-4 0.0 4.425487443649473 9.619355242101994E-4 104-105 0.009791129442853815 1.3741936060145707E-4 0.0 4.820430686018061 9.619355242101994E-4 106-107 0.010340806885259644 1.3741936060145707E-4 0.0 5.211629250810259 9.619355242101994E-4 108-109 0.0104782262458611 1.3741936060145707E-4 0.0 5.618356203350421 9.619355242101994E-4 110-111 0.010684355286763286 1.3741936060145707E-4 0.0 6.081493803417482 9.962903643605636E-4 112-113 0.010821774647364743 1.3741936060145707E-4 0.0 6.5894644698807685 0.001030645204510928 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4855 0.0 55.367752 1 GTATCAA 8020 0.0 45.92865 1 TCAACGC 10260 0.0 36.03965 4 ATCAACG 10340 0.0 35.760815 3 CAACGCA 10400 0.0 35.354782 5 TATCAAC 10425 0.0 35.183655 2 AACGCAG 10680 0.0 34.483585 6 GTACATG 8785 0.0 33.85543 1 TACATGG 9015 0.0 32.43038 2 ACATGGG 9005 0.0 30.94557 3 ACGCAGA 12300 0.0 29.69999 7 CGCAGAG 12445 0.0 29.401752 8 GCAGAGT 12995 0.0 27.195883 9 CATGGGA 5640 0.0 26.815811 4 GAGTACT 7680 0.0 26.610836 12-13 AGTACTT 8025 0.0 25.46682 12-13 GTACTTT 7985 0.0 25.408117 14-15 TATAGCG 120 0.0012884414 24.790298 5 ATGGGAT 2315 0.0 24.158495 5 AGAGTAC 12420 0.0 24.07174 10-11 >>END_MODULE