FastQCFastQC Report
Thu 26 May 2016
SRR1364561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179894
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57380.2632238081301201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42290.1940002587281767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32810.1505119056247689No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG30080.13798836090195213No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG29950.13739200162943702No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG29590.1357405451824722No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC28370.13014394277886907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23540.10798690211542396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61800.049.1031271
GTATCAA100750.045.475041
GTACATG137450.038.051481
TACATGG139250.036.703332
ATCAACG123800.036.2855573
TCAACGC123350.036.2732284
CAACGCA125550.035.5902255
AACGCAG127600.035.0594376
TATCAAC130600.034.6696132
ACATGGG142400.034.2618943
ACGCAGA145250.030.4305957
CGCAGAG144400.029.912118
CATGGGA96650.028.933674
CATGGGG49650.027.9218484
GCAGAGT150500.027.040659
GAGTACT64000.026.68696212-13
ATGGGAG39900.025.7976845
AGTACAT99500.024.1582682
ATGGGTA8800.023.6642575
GTACTTT71600.023.6049214-15
Produced by FastQC (version 0.11.5)