##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364561_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2179894 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.440595735388968 31.0 28.0 33.0 18.0 34.0 2 28.428571756241357 31.0 28.0 34.0 16.0 34.0 3 28.627117190101902 31.0 28.0 34.0 18.0 34.0 4 31.915133488142082 35.0 32.0 37.0 19.0 37.0 5 32.09615788657614 35.0 32.0 37.0 22.0 37.0 6 32.06402604897303 35.0 32.0 37.0 19.0 37.0 7 31.865816869994596 35.0 32.0 37.0 18.0 37.0 8 31.709422109515415 35.0 32.0 37.0 17.0 37.0 9 32.77744238940058 37.0 32.0 39.0 16.0 39.0 10-11 32.71590774597297 37.0 32.0 39.0 15.0 39.0 12-13 32.619545262292576 37.0 32.0 39.0 13.0 39.0 14-15 33.33067295932738 38.0 32.0 41.0 11.5 41.0 16-17 33.470046708693175 38.0 32.0 41.0 12.0 41.0 18-19 33.29634537275666 38.0 32.0 41.0 10.5 41.0 20-21 33.025943463306014 38.0 31.5 40.5 10.0 41.0 22-23 32.94229994669466 38.0 31.5 40.0 10.0 41.0 24-25 32.7776719877205 38.0 31.0 40.0 10.0 41.0 26-27 32.68515120459986 38.0 31.0 40.0 10.0 41.0 28-29 32.7534985646091 38.0 31.0 40.0 9.5 41.0 30-31 32.67257536375622 38.0 31.0 40.0 9.0 41.0 32-33 32.63784110603543 38.0 31.0 40.0 8.0 41.0 34-35 32.131734387084876 38.0 30.0 40.0 7.5 41.0 36-37 31.969698985363507 37.0 30.0 40.0 3.5 41.0 38-39 31.757560229992833 37.0 30.0 40.0 2.0 41.0 40-41 31.9423221954829 37.0 30.0 40.0 2.0 41.0 42-43 31.800649022383656 37.0 30.0 40.0 2.0 41.0 44-45 31.61674512613916 37.0 30.0 40.0 2.0 41.0 46-47 31.5073868270659 37.0 30.0 40.0 2.0 41.0 48-49 31.28902139278332 37.0 30.0 40.0 2.0 41.0 50-51 31.104092217327995 37.0 29.5 40.0 2.0 41.0 52-53 31.0139896710574 36.5 29.5 40.0 2.0 41.0 54-55 31.022024465409785 37.0 29.0 40.0 2.0 41.0 56-57 30.88996987926936 36.0 29.0 40.0 2.0 41.0 58-59 30.69020489069652 36.0 29.0 40.0 2.0 41.0 60-61 30.48344483722603 36.0 29.0 40.0 2.0 41.0 62-63 30.200942798136055 35.0 28.0 39.0 2.0 41.0 64-65 29.920972533526857 35.0 28.0 39.0 2.0 41.0 66-67 29.673800193954385 35.0 28.0 39.0 2.0 41.0 68-69 29.63694909018512 35.0 28.0 39.0 2.0 40.0 70-71 29.400648380150592 35.0 28.0 38.0 2.0 40.0 72-73 29.11918974041857 35.0 28.0 37.0 2.0 40.0 74-75 28.81618853944274 34.5 28.0 37.0 2.0 39.0 76-77 28.506345721397462 34.0 28.0 36.0 2.0 39.0 78-79 28.190437929550704 34.0 27.5 36.0 2.0 39.0 80-81 27.891259162142745 34.0 27.0 35.5 2.0 37.0 82-83 27.642905801841742 34.0 26.5 35.0 2.0 37.0 84-85 27.39795214813197 34.0 26.0 35.0 2.0 36.5 86-87 27.184300016422817 34.0 26.0 35.0 2.0 36.0 88-89 26.975778180039946 34.0 26.0 35.0 2.0 36.0 90-91 26.836340436736833 34.0 26.0 35.0 2.0 36.0 92-93 26.713917052847524 34.0 25.0 35.0 2.0 35.0 94-95 26.578107696979764 34.0 25.0 35.0 2.0 35.0 96-97 26.456676792541288 33.5 25.0 35.0 2.0 35.0 98-99 26.352531820354567 33.0 25.0 35.0 2.0 35.0 100-101 26.24674892448899 33.0 24.5 35.0 2.0 35.0 102-103 25.105881295145544 31.5 21.5 34.0 2.0 35.0 104-105 25.944936542786024 33.0 24.0 35.0 2.0 35.0 106-107 26.08183035505396 33.0 24.0 35.0 2.0 35.0 108-109 26.016435432181567 33.0 24.0 35.0 2.0 35.0 110-111 25.923063231514927 33.0 23.5 35.0 2.0 35.0 112-113 25.82570758027684 33.0 23.0 35.0 2.0 35.0 114-115 25.691275584959634 33.0 21.5 35.0 2.0 35.0 116-117 25.57000065140782 33.0 19.5 35.0 2.0 35.0 118-119 25.406073414578877 33.0 18.5 35.0 2.0 35.0 120-121 25.22110731072245 33.0 16.5 35.0 2.0 35.0 122-123 24.962030493225818 33.0 2.0 35.0 2.0 35.0 124-125 23.51570213964532 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1779177819630142 1101 2 1.1898736749944057 1101 3 1.1517255824784307 1101 4 1.2649984139989847 1101 5 1.1966719466195883 1101 6 1.2723957334690397 1101 7 1.411882050674965 1101 8 1.27720380286652 1101 9 1.6956679977395623 1101 10-11 1.6288424928224288 1101 12-13 1.3904048239145368 1101 14-15 1.774712352627219 1101 16-17 1.440560698730522 1101 18-19 1.8367993120606414 1101 20-21 2.2229893559124108 1101 22-23 2.128855664126 1101 24-25 2.313322166071437 1101 26-27 2.4292028375687593 1101 28-29 2.262112987925512 1101 30-31 2.355580624329015 1101 32-33 2.274949140411266 1101 34-35 2.6012130414118317 1101 36-37 2.4713238305365195 1101 38-39 2.339446130411446 1101 40-41 2.238597188640963 1101 42-43 2.249788938318943 1101 44-45 2.057937905570043 1101 46-47 1.9077241918860572 1101 48-49 1.9655772329144021 1101 50-51 1.8901108198355026 1101 52-53 1.7665344128560392 1101 54-55 1.689454612940704 1101 56-57 1.6268452443047678 1101 58-59 1.7331621377909308 1101 60-61 1.634845432297137 1101 62-63 1.6055308486350164 1101 64-65 1.6155333700252932 1101 66-67 1.6302687821521786 1101 68-69 1.4806004920287243 1101 70-71 1.3441485276167668 1101 72-73 1.1263549368111647 1101 74-75 1.2387957673506236 1101 76-77 1.4373280166729394 1101 78-79 1.417749085542571 1101 80-81 1.5163570815576541 1101 82-83 1.35378589182411 1101 84-85 1.4883444416812672 1101 86-87 1.470345162828231 1101 88-89 1.3739829822856784 1101 90-91 1.4128709208171202 1101 92-93 1.3629514930442639 1101 94-95 1.4513503464232294 1101 96-97 1.4049552087014945 1101 98-99 1.4214626259765701 1101 100-101 1.4214699278875038 1101 102-103 1.3403225231745672 1101 104-105 1.4337266123619479 1101 106-107 1.4347146467624157 1101 108-109 1.3821964502995847 1101 110-111 1.2621137388787709 1101 112-113 1.3095364133946816 1101 114-115 1.4206878963649459 1101 116-117 1.454841230758415 1101 118-119 1.5316809396742102 1101 120-121 1.5880131880820834 1101 122-123 1.5041095149488548 1101 124-125 1.74752139667671 1102 1 0.4786315249941211 1102 2 0.6286215596765246 1102 3 0.7560128560228527 1102 4 0.8966946275990857 1102 5 0.8475715013136451 1102 6 0.34674332892394943 1102 7 -0.04201280942742258 1102 8 0.5609116895053496 1102 9 0.9421634873544349 1102 10-11 0.9527847956641011 1102 12-13 1.0353795776815957 1102 14-15 1.3239077292722783 1102 16-17 1.0558755237648754 1102 18-19 1.328171604950576 1102 20-21 1.6019222802715447 1102 22-23 1.4994853447684164 1102 24-25 1.813164902601045 1102 26-27 1.4120548134705686 1102 28-29 1.7787500490591555 1102 30-31 1.6876081421878766 1102 32-33 1.5505739139747945 1102 34-35 2.055063760734562 1102 36-37 2.0473273740283773 1102 38-39 2.0769695766177847 1102 40-41 1.8866193759751972 1102 42-43 1.7462819438206836 1102 44-45 1.8416010781723386 1102 46-47 1.4302768818366296 1102 48-49 1.0409903218212015 1102 50-51 1.5397350690600753 1102 52-53 1.3492255710948058 1102 54-55 1.2150874121005337 1102 56-57 1.200934109821759 1102 58-59 1.2446805882546563 1102 60-61 1.1046275335358295 1102 62-63 1.1770933710875795 1102 64-65 1.2716123006184077 1102 66-67 1.214170276747513 1102 68-69 1.0449514429472373 1102 70-71 1.0704850569208944 1102 72-73 1.2002217608304733 1102 74-75 0.8347073757259835 1102 76-77 1.001580367408316 1102 78-79 0.9499514085323106 1102 80-81 0.775558427717673 1102 82-83 0.9216411620421141 1102 84-85 0.5645211087504904 1102 86-87 1.037522652329983 1102 88-89 1.1660925217387437 1102 90-91 0.6664853054373268 1102 92-93 1.0626273736050464 1102 94-95 0.9808140302177897 1102 96-97 1.0857174339776918 1102 98-99 1.2367909798732626 1102 100-101 1.24044801724526 1102 102-103 1.0685597812028398 1102 104-105 1.3524045826351383 1102 106-107 1.2514448048131577 1102 108-109 0.8098216228499062 1102 110-111 1.0585979926588251 1102 112-113 0.8204275297474872 1102 114-115 0.8853859734320721 1102 116-117 0.8904711712772801 1102 118-119 1.09142653591487 1102 120-121 1.4491659770914502 1102 122-123 1.3365920076837803 1102 124-125 1.5119795084144094 1103 1 0.9528059915363478 1103 2 0.9878474104368813 1103 3 0.8201258188117393 1103 4 1.1037206544681304 1103 5 1.0449580713954987 1103 6 1.0634808483237208 1103 7 1.1283556567579787 1103 8 1.217406704555163 1103 9 1.5112679228762147 1103 10-11 1.467581522498044 1103 12-13 1.3592467172966565 1103 14-15 1.7204708761311949 1103 16-17 1.7220459655137574 1103 18-19 1.6972225652033401 1103 20-21 2.052707719267538 1103 22-23 1.9344717913056115 1103 24-25 2.078361854654595 1103 26-27 2.1021809767375217 1103 28-29 1.9685438503402608 1103 30-31 1.8903290981626313 1103 32-33 1.954232773412059 1103 34-35 2.348452730728944 1103 36-37 2.2770209679309517 1103 38-39 2.3521343667741093 1103 40-41 2.005616765039189 1103 42-43 1.9490117723736944 1103 44-45 1.63666121027304 1103 46-47 1.6623642973552926 1103 48-49 1.6924738169072278 1103 50-51 1.6582008228263625 1103 52-53 1.6407614671484723 1103 54-55 1.5676830489022642 1103 56-57 1.6371057889356138 1103 58-59 1.4829625021992818 1103 60-61 1.4419986893114434 1103 62-63 1.3803580482504252 1103 64-65 1.5210179817542517 1103 66-67 1.5032076547615532 1103 68-69 1.6036517972748463 1103 70-71 1.5793916867032145 1103 72-73 1.4561102272460893 1103 74-75 1.4980017112801782 1103 76-77 1.5096373399119543 1103 78-79 1.4808414096641656 1103 80-81 1.4205152542314288 1103 82-83 1.4156620994084292 1103 84-85 1.4250568398221617 1103 86-87 1.4228734980545497 1103 88-89 1.388257661043017 1103 90-91 1.3764018744256958 1103 92-93 1.4696336889623751 1103 94-95 1.366350768446999 1103 96-97 1.4412657209466104 1103 98-99 1.362431867783343 1103 100-101 1.4175818962981097 1103 102-103 1.2866929811437515 1103 104-105 1.2957852076099563 1103 106-107 1.3513407318680954 1103 108-109 1.4480012863250025 1103 110-111 1.4924458715850584 1103 112-113 1.5154080484432058 1103 114-115 1.6034352435785806 1103 116-117 1.6610708520563016 1103 118-119 1.6789863179084286 1103 120-121 1.6849054417440534 1103 122-123 1.6560751823977924 1103 124-125 1.7785452355150078 1104 1 0.9346786786459838 1104 2 1.0178450525728593 1104 3 0.8797522625508876 1104 4 0.9548389653866671 1104 5 0.9881416946948178 1104 6 1.0200810346900155 1104 7 1.0318980193377278 1104 8 0.9825747623314278 1104 9 1.3431001732967616 1104 10-11 1.1628182751264262 1104 12-13 1.1116525758420153 1104 14-15 1.198767846615354 1104 16-17 1.351083355082224 1104 18-19 1.6164740497299235 1104 20-21 1.7393698572663894 1104 22-23 1.5634783611927503 1104 24-25 1.9760866290169616 1104 26-27 2.0294727775708665 1104 28-29 2.02832611223333 1104 30-31 1.849482068324555 1104 32-33 1.7821463533963708 1104 34-35 2.186762952318375 1104 36-37 2.187932538073781 1104 38-39 2.240949990406513 1104 40-41 1.906418710344898 1104 42-43 2.010674351623493 1104 44-45 1.8987418238788578 1104 46-47 1.7162019812720892 1104 48-49 1.696172314436442 1104 50-51 1.550264868024943 1104 52-53 1.5625720261251743 1104 54-55 1.4776850990220431 1104 56-57 1.4979851590736502 1104 58-59 1.509076955000591 1104 60-61 1.423561640886529 1104 62-63 1.4677322521318956 1104 64-65 1.3560704136805448 1104 66-67 1.3164393449970504 1104 68-69 1.4011095129888567 1104 70-71 1.4705065671785178 1104 72-73 1.3462965347088378 1104 74-75 1.3464545497004181 1104 76-77 1.3289367963360483 1104 78-79 1.3914118362565269 1104 80-81 1.3919896252451807 1104 82-83 1.358082969537186 1104 84-85 1.3821128044713973 1104 86-87 1.4511972377626563 1104 88-89 1.4890446264448691 1104 90-91 1.5252948776339323 1104 92-93 1.4108121322018903 1104 94-95 1.4304806872070408 1104 96-97 1.2584975885876162 1104 98-99 1.2515868699624448 1104 100-101 1.3993771495241099 1104 102-103 1.3379233019173498 1104 104-105 1.3744516069615251 1104 106-107 1.3512134705701477 1104 108-109 1.5173174197719597 1104 110-111 1.5042964689935836 1104 112-113 1.4393027148280773 1104 114-115 1.4276308657356225 1104 116-117 1.4401311640456136 1104 118-119 1.4458403615999629 1104 120-121 1.346514211006923 1104 122-123 1.3910601368720101 1104 124-125 1.6466000701092618 1105 1 0.30483810825969115 1105 2 0.491840517640842 1105 3 0.6499757678742668 1105 4 0.7299567908688225 1105 5 0.6871194916312362 1105 6 0.6490070667012731 1105 7 0.7373544436149793 1105 8 0.47148090748478566 1105 9 0.48068727203021666 1105 10-11 0.7100183638933331 1105 12-13 0.6487562323619329 1105 14-15 0.8405596794707648 1105 16-17 0.7425821919804463 1105 18-19 0.9209606080689454 1105 20-21 1.212227349156727 1105 22-23 1.4735666076741012 1105 24-25 1.5837250731216201 1105 26-27 1.3808691290403345 1105 28-29 1.1357284456496473 1105 30-31 1.0947219384842128 1105 32-33 1.1727133787935742 1105 34-35 1.712473888742295 1105 36-37 1.7423739943616567 1105 38-39 1.8876086938794288 1105 40-41 1.5716763303152312 1105 42-43 1.1248610065402715 1105 44-45 1.256807922909843 1105 46-47 1.2065685759212705 1105 48-49 1.1145054658142861 1105 50-51 1.0099444377018365 1105 52-53 0.979469767344824 1105 54-55 0.7798428204262429 1105 56-57 0.8662097754116402 1105 58-59 0.8058363112548399 1105 60-61 0.8979447024039438 1105 62-63 0.9050534339922045 1105 64-65 0.8972000921870524 1105 66-67 0.7009867083101611 1105 68-69 0.6252604585072739 1105 70-71 0.7154637740131733 1105 72-73 0.7967451584066936 1105 74-75 0.8446079766573682 1105 76-77 0.8771306031303752 1105 78-79 0.8459063884375695 1105 80-81 0.803485787710045 1105 82-83 0.8181077339330898 1105 84-85 0.7648755905346718 1105 86-87 0.7861840749265312 1105 88-89 0.9577866063807292 1105 90-91 0.8807055571390698 1105 92-93 0.7684936252212715 1105 94-95 0.8390838465555106 1105 96-97 0.9307599297897866 1105 98-99 0.8433816620123089 1105 100-101 0.8885287337464867 1105 102-103 0.8095834135740496 1105 104-105 0.9532868233928973 1105 106-107 0.9406355510525621 1105 108-109 0.9552218262758352 1105 110-111 1.0595681065296638 1105 112-113 1.014807699352339 1105 114-115 1.042599051494122 1105 116-117 1.0409421707849909 1105 118-119 1.1312308141406895 1105 120-121 0.8870811962340674 1105 122-123 1.0372413075936073 1105 124-125 1.370253487112965 1106 1 0.5294331971145212 1106 2 0.7174886489788364 1106 3 0.5106136050382268 1106 4 0.6720381821751893 1106 5 0.5331681153158883 1106 6 0.593883668368079 1106 7 0.29381020556285975 1106 8 0.6111340222606891 1106 9 0.7083863328606128 1106 10-11 0.8647266514076435 1106 12-13 0.7378121505624549 1106 14-15 0.9899251098370243 1106 16-17 0.8436926252335084 1106 18-19 0.9469255244625074 1106 20-21 1.2238162229800835 1106 22-23 1.25670050899069 1106 24-25 1.44172067231451 1106 26-27 1.6877377392514177 1106 28-29 1.5899037422852373 1106 30-31 1.3539330389276856 1106 32-33 1.2607357452014725 1106 34-35 1.7120011145564362 1106 36-37 1.4465073564812485 1106 38-39 1.7019072098428012 1106 40-41 1.3532138123215596 1106 42-43 1.2675519885021629 1106 44-45 1.0899893288092457 1106 46-47 0.7981204383317184 1106 48-49 0.9052750382385106 1106 50-51 0.8290438878371944 1106 52-53 0.8841280332967045 1106 54-55 0.7503153329189125 1106 56-57 0.8154706920610835 1106 58-59 0.7910013515165204 1106 60-61 0.879438859649202 1106 62-63 0.785211356744373 1106 64-65 0.7849674258624049 1106 66-67 0.5834043244778613 1106 68-69 0.6146520709134933 1106 70-71 0.8377328284121006 1106 72-73 0.8737070349794642 1106 74-75 0.830213034023604 1106 76-77 0.7250336138196367 1106 78-79 0.7169282203734788 1106 80-81 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100-101 -3.4703665478465915 2216 102-103 -3.4800099045104034 2216 104-105 -3.6073369107428697 2216 106-107 -3.550150907582289 2216 108-109 -3.6702289373792816 2216 110-111 -3.7787273530080476 2216 112-113 -3.7627217196095657 2216 114-115 -3.812601025366135 2216 116-117 -3.8943086042842054 2216 118-119 -3.9687854858179676 2216 120-121 -4.024862444503345 2216 122-123 -4.040038561875338 2216 124-125 -4.102417107145758 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 130665.0 3 23439.0 4 15628.0 5 12609.0 6 12333.0 7 12469.0 8 12758.0 9 13766.0 10 15060.0 11 16291.0 12 17847.0 13 20479.0 14 23940.0 15 21096.0 16 15063.0 17 13668.0 18 12376.0 19 11340.0 20 11390.0 21 11744.0 22 14159.0 23 18969.0 24 24462.0 25 31069.0 26 37687.0 27 41141.0 28 47343.0 29 56012.0 30 62731.0 31 71222.0 32 87669.0 33 109556.0 34 142345.0 35 193865.0 36 289310.0 37 387876.0 38 140056.0 39 461.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.05388780893559 18.93673257873731 13.384941693991752 22.624437918335342 2 15.29143472664031 21.048792505007334 40.03707676201784 23.62269600633452 3 18.62678980231315 27.002124135779532 28.79919805845732 25.57188800345 4 11.92552590687481 17.431576938410643 37.5520424496642 33.09085470505035 5 13.372846058660706 39.17770219646157 32.83777255040143 14.611679194476293 6 30.538617792938684 36.961139073965676 18.228789160484357 14.271453972611287 7 28.263003844495216 34.01754337673301 20.60420050097718 17.1152522777946 8 26.32251843438259 34.8210509318343 20.00982616585944 18.84660446792367 9 27.470103145752784 16.91720631169065 19.606868064819793 36.00582247773678 10-11 24.746960173292827 27.72894003102903 26.83316252992118 20.690937265756958 12-13 25.675365087316806 25.36783983639024 27.130828632259252 21.825966444033707 14-15 22.654685146715885 26.45983479909657 25.79921471643876 25.086265337748788 16-17 22.122663945155555 30.04460619732684 25.853055378819985 21.97967447869762 18-19 21.6854419957367 27.96992246646602 28.782532440974755 21.562103096822526 20-21 23.18227247567505 27.201506257837927 28.373636542351 21.242584724136023 22-23 22.433865273726468 27.238508897059177 28.13964909045326 22.18797673876109 24-25 22.297925658058634 27.40106008769374 28.46201948861986 21.83899476562776 26-27 22.24160010204358 27.725914801870736 28.205161734942724 21.827323361142962 28-29 21.818189324839913 27.49610873611107 28.79785291228933 21.887849026759692 30-31 21.86060327337911 27.292839096133577 28.74382843832682 22.102729192160496 32-33 22.172001543322448 27.354656324020638 28.2513110670992 22.22203106555771 34-35 22.610476912507355 27.248551691232386 28.169849256605563 21.9711221396547 36-37 22.905695357979415 26.983151143753553 28.09179574549691 22.019357752770123 38-39 22.181978156272315 27.11591185532688 28.317140263962436 22.38496972443837 40-41 22.33853810452782 27.11549893326548 28.422125397688124 22.123837564518567 42-43 22.650681674446517 27.100645304253696 27.870532665617425 22.378140355682362 44-45 22.261252258526884 27.464489855096403 27.6888055065386 22.58545237983811 46-47 22.3018288941559 27.625057086279593 27.22990878352263 22.843205236041875 48-49 22.735858558396718 27.392059166794574 27.223612939845516 22.648469334963192 50-51 22.71609146415033 27.651284554471705 26.746286212893665 22.886337768484292 52-53 22.875699460615976 27.714053986111253 26.751530120271905 22.65871643300087 54-55 22.4632429608799 27.591666913285067 26.79301328917602 23.15207683665901 56-57 22.524240800515276 27.955505928077674 26.858981299167446 22.661271972239604 58-59 22.756848851616894 27.210534838668536 27.07945030279037 22.9531660069242 60-61 22.81795002128628 27.46813022064329 27.15133626447834 22.562583493592093 62-63 22.45111288031919 27.48668696412306 27.184748916487518 22.877451239070233 64-65 22.349132937368232 27.13746596099537 27.142558907264792 23.370842194371605 66-67 22.513932164077495 27.810379439300352 26.65667514432199 23.019013252300162 68-69 22.321737265144918 27.533463873699326 27.110113895895775 23.034684965259974 70-71 22.297656239248013 27.60563897184643 27.06729423854154 23.02941055036402 72-73 22.486370794674716 27.64451316237708 26.831520885115516 23.037595157832676 74-75 22.328435386507504 27.814186292629962 26.59323186963764 23.2641464512249 76-77 22.464546685702334 27.71964722851239 26.78016651313351 23.035639572651768 78-79 22.617368291397327 27.723271444293214 26.703370707138824 22.955989557170636 80-81 22.28247692871959 28.485992309917265 26.581798822425974 22.649731938937173 82-83 22.598597659983582 28.04939224210401 26.38923708968916 22.962773008223248 84-85 22.63160502299653 27.573680188119237 26.35084549982706 23.443869289057172 86-87 22.655345019238208 27.42202528056769 26.656419441071264 23.26621025912284 88-89 22.677982992476274 27.34888089319323 26.782582842166985 23.19055327216351 90-91 22.394404100429412 27.853429975431105 26.834792348196036 22.91737357594345 92-93 22.37874553398575 27.35480751409765 26.867238952635937 23.399207999280666 94-95 22.401098879668595 27.348672894477406 26.764153157615677 23.48607506823833 96-97 22.611978219750196 27.491703936343125 26.668670186591175 23.227647657315504 98-99 22.35983230200319 27.518096254319634 26.51372489473001 23.60834654894716 100-101 22.090066002156718 27.48523134933843 26.8193832962908 23.605319352214053 102-103 22.59102965557041 26.903693482343638 26.91041399260698 23.594862869478973 104-105 22.124188538454025 27.03883225688954 27.003600684997174 23.833378519659263 106-107 22.350100509474313 27.376698133028487 26.79795439594769 23.475246961549505 108-109 22.522952950923305 27.391859420687425 26.366832515709483 23.718355112679788 110-111 21.825509599524924 27.637773476304933 26.89300548862124 23.643711435548898 112-113 22.06492609273662 27.32541123559219 26.866856828818282 23.74280584285291 114-115 21.54386864682411 27.66652873947082 26.708041767168496 24.081560846536576 116-117 21.607197414186192 27.726049982246842 26.9397961552259 23.726956448341067 118-119 21.8136294700568 27.871401086474844 26.378094531201974 23.936874912266376 120-121 21.65775529859601 27.88611003243966 26.660866689129538 23.795267979834794 122-123 21.69227035809998 27.71561369497783 26.61592719646001 23.97618875046218 124-125 21.70971850813164 27.832400655596896 26.436825384006884 24.02105545226458 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2700.0 1 2507.0 2 2949.0 3 3236.0 4 2384.0 5 1645.0 6 1338.5 7 1410.5 8 1814.5 9 2070.0 10 1825.0 11 1627.5 12 1735.0 13 1864.0 14 2085.5 15 2366.0 16 2653.0 17 2939.0 18 3157.5 19 3365.5 20 3730.0 21 4428.5 22 5416.0 23 6570.5 24 7765.0 25 9351.0 26 11893.0 27 14918.0 28 17511.0 29 20384.5 30 24074.5 31 27825.0 32 32461.0 33 37377.0 34 42259.5 35 45823.5 36 50257.0 37 56423.5 38 58874.5 39 59221.0 40 59753.5 41 64571.5 42 71339.5 43 75495.0 44 76471.5 45 75982.0 46 74561.5 47 73009.0 48 79451.5 49 82784.0 50 78851.5 51 75846.5 52 73895.0 53 73783.5 54 73081.5 55 68769.5 56 61262.0 57 55158.5 58 50200.0 59 44088.0 60 38895.5 61 33897.5 62 28699.0 63 23162.5 64 18589.5 65 15211.0 66 11742.0 67 10028.0 68 7478.0 69 4575.5 70 3600.5 71 3031.5 72 2733.5 73 2423.5 74 1844.5 75 1462.0 76 1077.5 77 686.0 78 435.0 79 290.5 80 194.0 81 140.5 82 98.0 83 69.0 84 64.5 85 51.0 86 31.0 87 30.5 88 24.0 89 16.5 90 15.5 91 9.0 92 5.5 93 7.0 94 5.5 95 4.0 96 4.0 97 3.0 98 2.5 99 2.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012523544722816799 2 0.004220388697799067 3 0.008440777395598134 4 0.008394903605404666 5 0.015734710036359567 6 8.257282234824262E-4 7 0.007064563689794091 8 0.0 9 0.003027670152768896 10-11 0.0 12-13 0.003348786684123173 14-15 0.019565171517514156 16-17 0.03268507551284604 18-19 0.005848908249667185 20-21 0.008601335661275274 22-23 0.0020413836636093315 24-25 0.012064806820882117 26-27 0.019863351153771698 28-29 5.046116921281494E-4 30-31 0.0016743933420615865 32-33 0.00830315602501773 34-35 0.009014199773016486 36-37 0.011491384443463764 38-39 2.293689509673406E-5 40-41 0.026583861417114777 42-43 0.010298665898433593 44-45 0.017248545112744016 46-47 0.0036469663203807156 48-49 0.02584988077401929 50-51 0.005091990711474962 52-53 0.0 54-55 0.018968812244999068 56-57 0.004495631438959876 58-59 1.3762137058040435E-4 60-61 0.003945145956638259 62-63 0.02931335193362613 64-65 0.01876238018912846 66-67 0.0021101943488995335 68-69 0.022546967880089582 70-71 0.0029359225723819596 72-73 0.01337220984139596 74-75 4.587379019346812E-5 76-77 0.005780097564376984 78-79 0.001146844754836703 80-81 0.003371723579219907 82-83 0.0017202671322550547 84-85 0.0 86-87 0.00536723345263577 88-89 0.017799030595065633 90-91 0.023510317474152415 92-93 0.004495631438959876 94-95 0.02676735657788865 96-97 0.01818895781171011 98-99 0.0010550971744497668 100-101 0.008830704612242612 102-103 0.0 104-105 0.0014220874959975118 106-107 0.0 108-109 0.0 110-111 9.404126989660965E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.293689509673406E-5 122-123 0.0 124-125 0.023212137837894872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2179894.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.53895377908969 #Duplication Level Percentage of deduplicated Percentage of total 1 74.35166235143996 33.858969152207074 2 11.907689718921905 10.845274634514524 3 4.966883941926721 6.785600947725112 4 2.6035114077755015 4.742447426480852 5 1.583903770625189 3.606466030051318 6 0.9982661543276362 2.72759977566935 7 0.6787153434342227 2.1635590657667034 8 0.4852204029249537 1.7677143601176597 9 0.36644899268750525 1.501893336635125 >10 1.8157300909975143 14.591248839381215 >50 0.13473522663574405 4.257961128605281 >100 0.0976097917154611 8.654156697487272 >500 0.007530892112150024 2.3592029701464736 >1k 0.0019873187518173667 1.864594376526425 >5k 1.0459572377986139E-4 0.2733112586856544 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5738 0.2632238081301201 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4229 0.1940002587281767 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3281 0.1505119056247689 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 3008 0.13798836090195213 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2995 0.13739200162943702 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2959 0.1357405451824722 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2837 0.13014394277886907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2354 0.10798690211542396 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.5873790193468126E-5 0.0 6 0.0 0.0 0.0 4.5873790193468126E-5 0.0 7 0.0 0.0 0.0 4.5873790193468126E-5 0.0 8 0.0 0.0 0.0 4.5873790193468126E-5 0.0 9 0.0 0.0 0.0 4.5873790193468126E-5 0.0 10-11 0.0 0.0 0.0 4.5873790193468126E-5 0.0 12-13 0.0 0.0 0.0 6.881068529020219E-5 0.0 14-15 0.0 0.0 0.0 1.1468447548367032E-4 0.0 16-17 0.0 0.0 0.0 3.4405342645101093E-4 0.0 18-19 0.0 0.0 0.0 4.128641117412131E-4 0.0 20-21 2.2936895096734063E-5 0.0 0.0 7.3398064309549E-4 0.0 22-23 4.5873790193468126E-5 0.0 0.0 0.001123907859739969 0.0 24-25 4.5873790193468126E-5 0.0 0.0 0.0017432040273517887 0.0 26-27 4.5873790193468126E-5 0.0 0.0 0.0024771846704472788 0.0 28-29 4.5873790193468126E-5 0.0 0.0 0.0037845876909611198 0.0 30-31 4.5873790193468126E-5 0.0 0.0 0.006353519941795335 0.0 32-33 4.5873790193468126E-5 0.0 0.0 0.01220242819146252 0.0 34-35 6.881068529020219E-5 0.0 0.0 0.020115656999835772 0.0 36-37 9.174758038693625E-5 0.0 0.0 0.034795269861745574 0.0 38-39 9.174758038693625E-5 0.0 0.0 0.05956711656621835 0.0 40-41 9.174758038693625E-5 0.0 0.0 0.0890869005557151 0.0 42-43 9.174758038693625E-5 0.0 0.0 0.11438629584741276 0.0 44-45 9.174758038693625E-5 0.0 0.0 0.14496117701135924 0.0 46-47 9.174758038693625E-5 0.0 0.0 0.18590353475902957 0.0 48-49 9.174758038693625E-5 0.0 0.0 0.23159382979172383 0.0 50-51 9.174758038693625E-5 0.0 0.0 0.28285779033292446 0.0 52-53 9.174758038693625E-5 0.0 0.0 0.3322867992663864 0.0 54-55 9.174758038693625E-5 0.0 0.0 0.38607381826822773 0.0 56-57 9.174758038693625E-5 0.0 0.0 0.4508476100214047 0.0 58-59 9.174758038693625E-5 0.0 0.0 0.5215849944997326 0.0 60-61 9.174758038693625E-5 0.0 0.0 0.6073460452664212 0.0 62-63 9.174758038693625E-5 0.0 0.0 0.6940245718369793 0.0 64-65 9.174758038693625E-5 0.0 0.0 0.7784552826880573 0.0 66-67 9.174758038693625E-5 0.0 0.0 0.8798592959107185 0.0 68-69 9.174758038693625E-5 0.0 0.0 0.997043892959933 0.0 70-71 9.174758038693625E-5 0.0 0.0 1.1348487587011111 0.0 72-73 9.174758038693625E-5 0.0 0.0 1.278204353055699 0.0 74-75 9.174758038693625E-5 0.0 0.0 1.4225462338994466 0.0 76-77 9.174758038693625E-5 0.0 0.0 1.5799850818434291 0.0 78-79 9.174758038693625E-5 0.0 0.0 1.7636637377780753 0.0 80-81 9.174758038693625E-5 0.0 0.0 1.97502722609448 0.0 82-83 9.174758038693625E-5 0.0 0.0 2.201391443804148 0.0 84-85 9.174758038693625E-5 0.0 0.0 2.4261959526472388 0.0 86-87 9.174758038693625E-5 0.0 0.0 2.660863326381925 0.0 88-89 9.174758038693625E-5 0.0 0.0 2.9233990276591433 4.5873790193468126E-5 90-91 9.174758038693625E-5 0.0 0.0 3.2209364308539774 4.5873790193468126E-5 92-93 9.174758038693625E-5 0.0 0.0 3.5494386424294024 4.5873790193468126E-5 94-95 9.174758038693625E-5 0.0 0.0 3.8826199806045616 4.5873790193468126E-5 96-97 9.174758038693625E-5 0.0 0.0 4.22353105242732 6.881068529020219E-5 98-99 9.174758038693625E-5 0.0 0.0 4.58662210180862 9.174758038693625E-5 100-101 9.174758038693625E-5 0.0 0.0 5.017147622774319 9.174758038693625E-5 102-103 9.174758038693625E-5 0.0 0.0 5.4798536075607345 9.174758038693625E-5 104-105 9.174758038693625E-5 0.0 0.0 5.938041024013094 9.174758038693625E-5 106-107 9.174758038693625E-5 0.0 0.0 6.40074700879951 9.174758038693625E-5 108-109 9.174758038693625E-5 0.0 0.0 6.886573383843435 9.174758038693625E-5 110-111 9.174758038693625E-5 0.0 0.0 7.423663719428559 9.174758038693625E-5 112-113 9.174758038693625E-5 0.0 0.0 8.010412432898114 9.174758038693625E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6180 0.0 49.103127 1 GTATCAA 10075 0.0 45.47504 1 GTACATG 13745 0.0 38.05148 1 TACATGG 13925 0.0 36.70333 2 ATCAACG 12380 0.0 36.285557 3 TCAACGC 12335 0.0 36.273228 4 CAACGCA 12555 0.0 35.590225 5 AACGCAG 12760 0.0 35.059437 6 TATCAAC 13060 0.0 34.669613 2 ACATGGG 14240 0.0 34.261894 3 ACGCAGA 14525 0.0 30.430595 7 CGCAGAG 14440 0.0 29.91211 8 CATGGGA 9665 0.0 28.93367 4 CATGGGG 4965 0.0 27.921848 4 GCAGAGT 15050 0.0 27.04065 9 GAGTACT 6400 0.0 26.686962 12-13 ATGGGAG 3990 0.0 25.797684 5 AGTACAT 9950 0.0 24.158268 2 ATGGGTA 880 0.0 23.664257 5 GTACTTT 7160 0.0 23.60492 14-15 >>END_MODULE