FastQCFastQC Report
Thu 26 May 2016
SRR1364561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179894
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124460.5709451927479042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105510.48401436033128215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66600.3055194426884977No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC35300.16193447938294248No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG33960.15578739149701776No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.13358447704337917No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG28650.13142840890428617No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG26630.12216190328520561No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG24580.11275777629554465No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82400.057.2413641
GTATCAA140150.048.7161561
ATCAACG179050.038.0901643
TCAACGC180800.037.721484
TATCAAC181250.037.364932
CAACGCA183700.037.183135
AACGCAG186950.036.6958586
GTACATG133250.032.801781
ACGCAGA214050.031.7163817
CGCAGAG213800.031.5586598
TACATGG135650.031.3516312
ACATGGG138650.029.642233
GAGTACT130400.029.04050312-13
GCAGAGT223400.028.8422169
AGTACTT132450.026.79426412-13
GTACTTT142450.026.77188114-15
CATGGGG46750.026.2462524
CATGGGA93450.025.1767374
CAGAGTA221650.024.7880610-11
AGAGTAC215600.023.8969510-11
Produced by FastQC (version 0.11.5)