##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364561_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2179894 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.92927821261034 31.0 30.0 34.0 26.0 34.0 2 29.746296838286632 31.0 30.0 34.0 26.0 34.0 3 30.010811993610698 31.0 30.0 34.0 26.0 34.0 4 33.53754035746692 35.0 33.0 37.0 28.0 37.0 5 32.94676621890789 35.0 33.0 37.0 26.0 37.0 6 32.96972283973441 35.0 33.0 37.0 26.0 37.0 7 32.745355508111864 35.0 33.0 37.0 25.0 37.0 8 32.64877558266595 35.0 33.0 37.0 25.0 37.0 9 33.80239268514891 38.0 33.0 39.0 23.0 39.0 10-11 33.74452014639244 38.0 33.0 39.0 23.0 39.0 12-13 33.62208575279348 37.5 33.0 39.0 21.5 39.0 14-15 34.530295051043765 38.0 33.0 41.0 20.5 41.0 16-17 34.55304936845553 38.0 33.0 41.0 22.0 41.0 18-19 34.61087672152866 38.5 33.5 41.0 22.0 41.0 20-21 34.65257462060082 39.0 34.0 41.0 22.0 41.0 22-23 34.62166210834105 39.0 33.5 41.0 23.0 41.0 24-25 34.58381554332458 39.0 33.0 41.0 23.0 41.0 26-27 34.44897733559522 39.0 33.0 41.0 22.5 41.0 28-29 34.2873458525965 38.0 33.0 41.0 21.5 41.0 30-31 34.07066123398661 38.0 33.0 40.0 20.5 41.0 32-33 33.80617360293665 38.0 33.0 40.0 18.5 41.0 34-35 33.58133308316826 38.0 33.0 40.0 17.5 41.0 36-37 33.335598428180454 38.0 32.0 40.0 16.0 41.0 38-39 33.14013112564189 38.0 32.0 40.0 15.0 41.0 40-41 33.128251648933386 38.0 32.0 40.0 14.0 41.0 42-43 32.95228529460607 38.0 32.0 40.0 9.0 41.0 44-45 32.72295854752571 38.0 31.0 40.0 7.5 41.0 46-47 32.37891704826015 38.0 31.0 40.0 2.0 41.0 48-49 32.00219712518132 37.5 30.0 40.0 2.0 41.0 50-51 31.79728234492136 37.0 30.0 40.0 2.0 41.0 52-53 31.601174644271694 37.0 30.0 40.0 2.0 41.0 54-55 31.40195326011265 37.0 30.0 40.0 2.0 41.0 56-57 31.192448577774883 36.0 29.5 40.0 2.0 41.0 58-59 30.98232299368685 36.0 29.0 40.0 2.0 41.0 60-61 30.744256601467775 36.0 29.0 40.0 2.0 41.0 62-63 30.575752307222277 35.0 29.0 39.0 2.0 41.0 64-65 30.448004811243116 35.0 29.0 39.0 2.0 41.0 66-67 30.155715140277465 35.0 29.0 39.0 2.0 41.0 68-69 29.876707307786525 35.0 29.0 39.0 2.0 40.5 70-71 29.567986333280427 35.0 28.0 38.0 2.0 40.0 72-73 29.28740732347536 35.0 28.0 37.0 2.0 40.0 74-75 28.94064894898559 35.0 28.0 37.0 2.0 39.0 76-77 28.634503329060955 34.0 28.0 36.0 2.0 39.0 78-79 28.347765304184517 34.0 27.5 36.0 2.0 38.5 80-81 28.06884073262278 34.0 27.0 35.5 2.0 37.0 82-83 27.788558755609216 34.0 26.5 35.0 2.0 37.0 84-85 27.561749791503622 34.0 26.0 35.0 2.0 36.5 86-87 27.332910912181966 34.0 26.0 35.0 2.0 36.0 88-89 27.150129088845603 34.0 26.0 35.0 2.0 36.0 90-91 27.004912853560768 34.0 26.0 35.0 2.0 35.5 92-93 26.896323169842205 34.0 25.5 35.0 2.0 35.0 94-95 26.777746073891663 34.0 25.0 35.0 2.0 35.0 96-97 26.656242230126786 34.0 25.0 35.0 2.0 35.0 98-99 26.561311696807273 34.0 25.0 35.0 2.0 35.0 100-101 26.455063640709135 34.0 25.0 35.0 2.0 35.0 102-103 25.192207511007418 31.5 21.5 34.0 2.0 35.0 104-105 26.113826406238104 33.0 24.0 35.0 2.0 35.0 106-107 26.269345206693536 33.0 24.0 35.0 2.0 35.0 108-109 26.256820285757016 34.0 24.0 35.0 2.0 35.0 110-111 26.174911945259723 33.0 24.0 35.0 2.0 35.0 112-113 26.09371877715155 33.0 24.0 35.0 2.0 35.0 114-115 25.981386709629 33.0 23.5 35.0 2.0 35.0 116-117 25.851234738936846 33.0 23.0 35.0 2.0 35.0 118-119 25.702414658694416 33.0 21.5 35.0 2.0 35.0 120-121 25.499446991459216 33.0 19.5 35.0 2.0 35.0 122-123 25.191475824053832 33.0 10.0 35.0 2.0 35.0 124-125 23.41618697973388 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6527197977685191 1101 2 0.7778519729948243 1101 3 0.6399033214229455 1101 4 0.48910516847437435 1101 5 0.6566807603089515 1101 6 0.6196429643487633 1101 7 0.8366057255885551 1101 8 0.8846118194760351 1101 9 1.1274125901281806 1101 10-11 1.054196600718818 1101 12-13 0.9268931178992403 1101 14-15 1.132815737637138 1101 16-17 1.1123099324481913 1101 18-19 1.1843987895383563 1101 20-21 1.1887956408558225 1101 22-23 1.1544175904090466 1101 24-25 1.1495567050499318 1101 26-27 1.2164831360958672 1101 28-29 1.3450846900268019 1101 30-31 1.3646586352412626 1101 32-33 1.4121597440502285 1101 34-35 1.5140079917819378 1101 36-37 1.4436477973976594 1101 38-39 1.432173517513668 1101 40-41 1.207998028128351 1101 42-43 1.2334007998725056 1101 44-45 1.3156410927121271 1101 46-47 1.1805855062051336 1101 48-49 1.2480994632531086 1101 50-51 1.3402963159022754 1101 52-53 1.3967969927333783 1101 54-55 1.386756447663938 1101 56-57 1.3763772193058301 1101 58-59 1.3727779822271948 1101 60-61 1.260035402621181 1101 62-63 1.24018063868742 1101 64-65 1.1448201987879223 1101 66-67 1.2565229114118068 1101 68-69 1.287805380481327 1101 70-71 1.3420740392503028 1101 72-73 1.2506537316752997 1101 74-75 1.358871437774262 1101 76-77 1.2922445197540284 1101 78-79 1.1646306857506694 1101 80-81 0.9997664092394416 1101 82-83 1.1971196240701438 1101 84-85 1.110920821771412 1101 86-87 1.2025531149835231 1101 88-89 1.2498839692412034 1101 90-91 1.1716076758438412 1101 92-93 1.092754105050151 1101 94-95 1.098426625074211 1101 96-97 1.1617563790841814 1101 98-99 1.1706478842597967 1101 100-101 1.1434055477450116 1101 102-103 1.0305629447999465 1101 104-105 1.062817541819438 1101 106-107 0.9797004188466474 1101 108-109 0.9579831075091967 1101 110-111 0.9167334233265549 1101 112-113 0.9460609291971238 1101 114-115 1.0459055342639338 1101 116-117 1.0758635589522 1101 118-119 1.024318345903172 1101 120-121 1.018471228510581 1101 122-123 0.9787034897826494 1101 124-125 1.4189764288235054 1102 1 0.3720524656143098 1102 2 -0.30121696611306703 1102 3 0.17012886360930324 1102 4 0.28314811229367365 1102 5 0.440737951472542 1102 6 0.37664166649519615 1102 7 0.46571120026818136 1102 8 0.651157541680341 1102 9 0.8866424080465123 1102 10-11 0.7848436395795133 1102 12-13 0.8521759628431909 1102 14-15 1.0223766534417607 1102 16-17 0.9773008673460808 1102 18-19 0.9308484186029347 1102 20-21 0.8538915199796477 1102 22-23 0.9897606349686896 1102 24-25 0.9357775587593622 1102 26-27 1.08915552208272 1102 28-29 0.7825265044536138 1102 30-31 0.9206299081925593 1102 32-33 1.0193757869879292 1102 34-35 1.0995809123499356 1102 36-37 0.9122075238853284 1102 38-39 0.41786445256442306 1102 40-41 0.05506513128554502 1102 42-43 0.24947396732313365 1102 44-45 -0.10473925940517859 1102 46-47 0.25542495606197235 1102 48-49 -0.05224319149185774 1102 50-51 -0.04286609770791827 1102 52-53 -0.20737887789094955 1102 54-55 -0.22913201642036896 1102 56-57 -0.29161761985357515 1102 58-59 -0.06000745765956239 1102 60-61 -0.24837542230310916 1102 62-63 -0.1080136785789243 1102 64-65 -0.21846854535148097 1102 66-67 -0.36360478729049106 1102 68-69 -0.08688334103354123 1102 70-71 -0.5394094960477993 1102 72-73 -0.40734503620458185 1102 74-75 -0.2548583319904907 1102 76-77 -0.6550951479404254 1102 78-79 -0.38359512242687543 1102 80-81 -0.3910675190071444 1102 82-83 -0.5988683803385868 1102 84-85 -0.5464110572729162 1102 86-87 -0.15974160172821428 1102 88-89 -0.3075483444082714 1102 90-91 -0.3978808032215788 1102 92-93 -0.18801431140033387 1102 94-95 -0.14803580672732863 1102 96-97 -0.35806302206875174 1102 98-99 -0.4834584694137085 1102 100-101 -0.36070546841777684 1102 102-103 -0.32220596235842436 1102 104-105 -0.26977425189369697 1102 106-107 -0.12155451689507757 1102 108-109 -0.0931924894805185 1102 110-111 0.002677814351947916 1102 112-113 0.14059450447033583 1102 114-115 0.11623612768417146 1102 116-117 -0.06912094018179715 1102 118-119 0.23448005020351914 1102 120-121 0.42878786503667143 1102 122-123 0.3649884336111171 1102 124-125 0.5905388366246918 1103 1 0.25010976424515263 1103 2 -0.14703898890743972 1103 3 0.31315453749360955 1103 4 0.3074064127105416 1103 5 0.3825782489005505 1103 6 0.48929578557766007 1103 7 0.5787473677747883 1103 8 0.6286822937757037 1103 9 0.7301242763333278 1103 10-11 0.7551755123745565 1103 12-13 0.8404273743025357 1103 14-15 0.7818408168583417 1103 16-17 0.6168671469445286 1103 18-19 0.7820255939193288 1103 20-21 0.6939280462261692 1103 22-23 0.8035900109722434 1103 24-25 0.7602723013970447 1103 26-27 0.8879545586646032 1103 28-29 0.9052291781211466 1103 30-31 0.8837283755865499 1103 32-33 0.9633537043396316 1103 34-35 0.8223587871132594 1103 36-37 1.087757767617461 1103 38-39 1.057409437975032 1103 40-41 1.001812444093325 1103 42-43 0.8953731096085917 1103 44-45 0.8729288927389192 1103 46-47 0.9540918631675197 1103 48-49 0.826895842289165 1103 50-51 0.8590036662719136 1103 52-53 0.8724325054830828 1103 54-55 0.8788536397379652 1103 56-57 0.8527303559516213 1103 58-59 0.9045984368018409 1103 60-61 1.011708910354308 1103 62-63 0.9243719035293552 1103 64-65 0.916388457462805 1103 66-67 0.7975223370925022 1103 68-69 0.8198600582478264 1103 70-71 0.815810169806106 1103 72-73 0.8741345837229417 1103 74-75 0.8592541032396888 1103 76-77 0.8529478682374041 1103 78-79 0.7297406504658142 1103 80-81 0.7823941677611082 1103 82-83 0.8875742721114932 1103 84-85 0.8901604633814877 1103 86-87 0.9084070892547516 1103 88-89 0.9180147525723648 1103 90-91 0.8841188457120772 1103 92-93 0.8453315234757071 1103 94-95 0.8344408032379675 1103 96-97 0.8905600976640535 1103 98-99 0.9209772921604475 1103 100-101 0.8145990768362097 1103 102-103 0.6389403459775806 1103 104-105 0.675730276478415 1103 106-107 0.8384777792898106 1103 108-109 0.8656494903801679 1103 110-111 0.8500026121051221 1103 112-113 0.767097759027962 1103 114-115 0.9091280894160185 1103 116-117 0.8828396118556299 1103 118-119 0.8420374725708548 1103 120-121 0.8453915284043383 1103 122-123 0.8827598721582 1103 124-125 0.9428545795520478 1104 1 0.4270665990412503 1104 2 0.5773015445853176 1104 3 0.30201247596014724 1104 4 0.013397604889128445 1104 5 0.4329672059507672 1104 6 0.5063168484086304 1104 7 0.6611015388633916 1104 8 0.4055678043166253 1104 9 0.5344172180832203 1104 10-11 0.5686922733482405 1104 12-13 0.5658549690956818 1104 14-15 0.7276402662676773 1104 16-17 0.6102286509449755 1104 18-19 0.5656276679345353 1104 20-21 0.4452019037595889 1104 22-23 0.5447559634158097 1104 24-25 0.7505946951766234 1104 26-27 0.6901135203355082 1104 28-29 0.9231336479705803 1104 30-31 0.8241079057063345 1104 32-33 0.7446109482092922 1104 34-35 1.0808089237200278 1104 36-37 1.1088521876258994 1104 38-39 1.1617416171165758 1104 40-41 1.0465735282039006 1104 42-43 0.9563023524174454 1104 44-45 0.9391390268475206 1104 46-47 0.8772944217894008 1104 48-49 0.8835457203002512 1104 50-51 0.8697686053317533 1104 52-53 0.8944029104428353 1104 54-55 0.5384183083449336 1104 56-57 0.6936989900242523 1104 58-59 0.8111757912603075 1104 60-61 0.8759960954765056 1104 62-63 0.9484909969329038 1104 64-65 0.8953929681788679 1104 66-67 0.7955536337774198 1104 68-69 0.5981725064574128 1104 70-71 0.247411974092536 1104 72-73 0.2890436158808569 1104 74-75 0.71049134825887 1104 76-77 0.860511995576541 1104 78-79 0.9928056836139447 1104 80-81 0.9338178695895181 1104 82-83 0.936029373357087 1104 84-85 0.9107267852086558 1104 86-87 0.9484523548131456 1104 88-89 0.8922113477443112 1104 90-91 1.0061738803400964 1104 92-93 0.9324173981504842 1104 94-95 0.8922965861370713 1104 96-97 0.9517866304680069 1104 98-99 0.9070586081472634 1104 100-101 0.8635821857500687 1104 102-103 0.7469326903868385 1104 104-105 0.8107783637800274 1104 106-107 0.716509863437949 1104 108-109 0.83598597703606 1104 110-111 0.7968754553497064 1104 112-113 0.7905002156416785 1104 114-115 0.8277123120020029 1104 116-117 0.7730033363213451 1104 118-119 0.7667922841295471 1104 120-121 0.7647579644589335 1104 122-123 0.8541316919268773 1104 124-125 1.2793418237792409 1105 1 0.31097048262020266 1105 2 0.19824703533089405 1105 3 0.3483054354964885 1105 4 0.4490668153323085 1105 5 -0.09665111688136818 1105 6 0.5082829496980636 1105 7 0.5706150856806573 1105 8 0.747515532713102 1105 9 0.9118194881201163 1105 10-11 0.7442723044720054 1105 12-13 0.7755877194691934 1105 14-15 0.8496153602946279 1105 16-17 0.8391529154735693 1105 18-19 0.8697198455472659 1105 20-21 0.822678354508021 1105 22-23 0.7795467974649881 1105 24-25 0.7746307186155903 1105 26-27 0.8089486546414264 1105 28-29 0.8257028625468124 1105 30-31 0.8267830978285744 1105 32-33 0.8945561874902879 1105 34-35 0.9732780461104369 1105 36-37 1.1006413826473533 1105 38-39 1.0823812440956075 1105 40-41 0.7523097146351176 1105 42-43 0.7347233173326444 1105 44-45 0.7338786324650144 1105 46-47 0.6237804564893707 1105 48-49 0.6554757836895 1105 50-51 0.5729617145060324 1105 52-53 0.7338856700331533 1105 54-55 0.6125021207657504 1105 56-57 0.637981107750555 1105 58-59 0.4254453750350997 1105 60-61 0.5363294909097753 1105 62-63 0.6710618308089025 1105 64-65 0.4967881814966155 1105 66-67 0.6446017327828741 1105 68-69 0.7550660949854873 1105 70-71 0.730621960048186 1105 72-73 0.8364251394205553 1105 74-75 0.7304527439381623 1105 76-77 0.779821279422027 1105 78-79 0.6883975907928956 1105 80-81 0.7492649628080699 1105 82-83 0.7074899380070967 1105 84-85 0.6440494928171248 1105 86-87 0.8284934853843211 1105 88-89 0.7016441348868199 1105 90-91 0.6365444277232264 1105 92-93 0.6822941876485693 1105 94-95 0.562106277351603 1105 96-97 0.6123936286459788 1105 98-99 0.4375746871958732 1105 100-101 0.47436982868964606 1105 102-103 0.15414601027897135 1105 104-105 0.3686548958326519 1105 106-107 0.38525250394813426 1105 108-109 0.361303068334486 1105 110-111 0.36395039563245035 1105 112-113 0.3735368186899777 1105 114-115 0.3921168359363527 1105 116-117 0.2288338554443925 1105 118-119 0.29928923812313357 1105 120-121 0.30176552526770095 1105 122-123 0.21680422568378432 1105 124-125 0.6654965697424373 1106 1 -0.12871032801806237 1106 2 -0.5393966392267302 1106 3 -0.12087201374729872 1106 4 -0.17594465646106983 1106 5 0.1686524372210627 1106 6 0.18190265897510471 1106 7 0.2546150188035128 1106 8 0.3133393655841985 1106 9 0.37144813458297676 1106 10-11 0.3131559485932556 1106 12-13 0.20198151283371146 1106 14-15 0.24444123164662557 1106 16-17 0.31955677530270776 1106 18-19 0.32737493465288736 1106 20-21 0.33094822270006574 1106 22-23 0.254689034073067 1106 24-25 0.2927029957297762 1106 26-27 0.4952178220772723 1106 28-29 0.545238004579879 1106 30-31 0.4646116295462477 1106 32-33 0.45068147092309374 1106 34-35 0.45544515465694957 1106 36-37 0.5561552217645556 1106 38-39 0.4162969356371278 1106 40-41 0.37191281511785235 1106 42-43 0.29498789641460377 1106 44-45 0.23527787322765192 1106 46-47 0.21663819516582805 1106 48-49 0.3260822049497456 1106 50-51 0.49717759357616487 1106 52-53 0.25250355018384596 1106 54-55 0.33551978123955095 1106 56-57 -0.04070581760345959 1106 58-59 0.3912002317608092 1106 60-61 0.4871896437058858 1106 62-63 0.35636491422705774 1106 64-65 0.34196054189829184 1106 66-67 0.19033738777093134 1106 68-69 0.2916861493704168 1106 70-71 0.3373322640538916 1106 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-2.1235726107645725 2216 50-51 -2.01924620727387 2216 52-53 -1.8962663794300418 2216 54-55 -1.9266043951529888 2216 56-57 -1.852738919314767 2216 58-59 -2.09326422662593 2216 60-61 -1.9762849116023098 2216 62-63 -2.0315244019257648 2216 64-65 -1.9465899089775967 2216 66-67 -1.875172293130369 2216 68-69 -1.7219233313914586 2216 70-71 -1.752865401735761 2216 72-73 -1.6474467289447539 2216 74-75 -1.6398922526930448 2216 76-77 -1.7131138997071282 2216 78-79 -1.7353604816897779 2216 80-81 -1.754736122760967 2216 82-83 -1.5674302968929616 2216 84-85 -1.5406015299950155 2216 86-87 -1.6786506592147887 2216 88-89 -1.6440475232706113 2216 90-91 -1.7075859653767615 2216 92-93 -1.718309608804777 2216 94-95 -1.6693307590285045 2216 96-97 -1.7294134250071629 2216 98-99 -1.6702306527034274 2216 100-101 -1.702017320373681 2216 102-103 -1.6531110433536398 2216 104-105 -1.5730059098287263 2216 106-107 -1.603073933722591 2216 108-109 -1.5783863456019418 2216 110-111 -1.6603818295500403 2216 112-113 -1.5976155801753436 2216 114-115 -1.7737794626591814 2216 116-117 -1.749298703045568 2216 118-119 -1.731587194641179 2216 120-121 -1.8696622484658825 2216 122-123 -1.8629503634525584 2216 124-125 -2.2767111672772273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74827.0 3 24776.0 4 11882.0 5 9673.0 6 11292.0 7 13577.0 8 15076.0 9 16192.0 10 17901.0 11 19822.0 12 22059.0 13 25637.0 14 30407.0 15 26473.0 16 17033.0 17 14781.0 18 13922.0 19 12703.0 20 12316.0 21 12594.0 22 14231.0 23 17468.0 24 22779.0 25 30042.0 26 37203.0 27 39950.0 28 44833.0 29 53312.0 30 60875.0 31 70783.0 32 85628.0 33 106479.0 34 136063.0 35 189158.0 36 290392.0 37 420719.0 38 156528.0 39 508.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42910068751182 19.479562916607737 14.428380664441697 22.662955731438743 2 15.8011811583499 21.373562200730863 38.75738912075541 24.067867520163823 3 18.503514391066723 26.72675827356743 29.877416057845014 24.892311277520836 4 12.515009681962347 18.05101661752598 36.70453277471679 32.72944092579489 5 13.001962480744478 39.11153478104899 33.014036462323396 14.87246627588314 6 30.55515543416331 37.43452663294637 17.470482509700012 14.539835423190302 7 27.431637435270716 34.35909292758027 20.63923723121979 17.57003240592922 8 26.69773723729441 34.62197141073206 19.417057495541957 19.263233856431576 9 27.331268332918928 16.04727358472389 19.691653722184654 36.92980436017253 10-11 25.08384072040745 27.248366020567673 26.519104857956467 21.14868840106841 12-13 26.22265119313141 24.778200224414583 27.515259916307855 21.483888666146154 14-15 23.112367645483598 25.554964038380774 26.60148305410333 24.731185262032295 16-17 21.941863460259725 29.08255961150253 26.82861095227249 22.14696597596525 18-19 21.468631478506 26.200717709111053 30.822750621888407 21.507900190494542 20-21 22.79190555749757 25.428145583260626 30.665554671709177 21.11439418753263 22-23 22.03845691579499 25.51738754269703 30.573963688142637 21.87019185336535 24-25 21.93948421345258 25.712052971383017 30.840077545054946 21.508385270109464 26-27 21.890720374476924 26.150812837688438 30.646146097012057 21.31232069082258 28-29 21.49113672499672 25.815498368269285 31.227091776022135 21.466273130711862 30-31 21.542010758321275 25.633677600837473 31.161859246367023 21.662452394474226 32-33 21.775668908671705 25.72765006004879 30.757390038231218 21.739290993048286 34-35 22.241861301512824 25.74083877472941 30.397028479366426 21.620271444391335 36-37 22.535912296652956 25.45249906646837 30.386936245523867 21.62465239135481 38-39 21.970150842196915 25.58064749937382 30.48987703071801 21.959324627711254 40-41 21.94269537876612 25.485161205086122 30.77922596236331 21.79291745378445 42-43 22.43159529775301 25.540576743639825 29.978567765221616 22.04926019338555 44-45 22.122979374226453 25.893116821276628 29.652473927631345 22.33142987686557 46-47 22.192501103264654 26.159849974356554 28.985354333742837 22.662294588635962 48-49 22.725760977368626 26.02436173502014 28.76261414545845 22.487263142152784 50-51 22.627894436127267 26.425832271163706 28.109171388418748 22.83710190429028 52-53 22.84666135142351 26.52218410619966 28.0943706437102 22.53678389866663 54-55 22.384070051112577 26.38444805114377 28.10989892169069 23.121582976052967 56-57 22.447034481493038 26.73903070119098 28.133682307819353 22.680252509496633 58-59 22.66133486491892 26.034829504063534 28.46485304668706 22.838982584330488 60-61 22.7800067342724 26.259671341817537 28.478380141419716 22.481941782490342 62-63 22.406639955887762 26.380181788655776 28.480582083349006 22.73259617210745 64-65 22.270422943553548 25.968730517930037 28.59473578700567 23.166110751510743 66-67 22.419553427827225 26.644712999806412 28.055721975472203 22.88001159689416 68-69 22.26837635224465 26.469452184372265 28.373145666715903 22.889025796667177 70-71 22.351499660075213 26.555534351670314 28.25616750172256 22.836798486531915 72-73 22.50877794975352 26.599091515459012 28.00243956816249 22.889690966624983 74-75 22.352742567255902 26.777742378023955 27.69603340024116 23.173481654478984 76-77 22.500405983043212 26.750543833782743 27.877571111255868 22.871479071918177 78-79 22.61307201175837 26.742584731184177 27.72923821066529 22.915105046392164 80-81 22.143523132448045 27.506012337764364 27.641730039440016 22.708734490347574 82-83 22.512447852968997 27.066499563740255 27.399245100908576 23.02180748238217 84-85 22.507837537054552 26.596017971516044 27.331328954527145 23.564815536902252 86-87 22.616145555701333 26.42646844296099 27.565078852457965 23.392307148879716 88-89 22.48272309168408 26.44272420184686 27.710121817428224 23.364430889040836 90-91 22.23731979628367 26.9313324409352 27.743665517681137 23.08768224509999 92-93 22.25349877077965 26.466038515723884 27.750607040801672 23.529855672694794 94-95 22.25321506458571 26.438670871152453 27.653340024790197 23.654774039471643 96-97 22.47558572356935 26.534412585845374 27.57564795637602 23.414353734209257 98-99 22.21861590025571 26.58424067374556 27.342287002469018 23.854856423529714 100-101 21.9853173248777 26.6030475519394 27.660850664816994 23.750784458365903 102-103 22.453752338416454 26.02424705054466 27.675909929565385 23.846090681473502 104-105 22.08914745395877 26.154161624372563 27.72529306470865 24.03139785696002 106-107 22.291164053421852 26.503481650401838 27.55669293903224 23.648661357144068 108-109 22.41053922805421 26.489476093791716 27.084963764293125 24.015020913860948 110-111 21.797287524304547 26.750449162469398 27.457480089022816 23.994783224203236 112-113 21.95613639929281 26.452387134420295 27.46493636846562 24.12654009782127 114-115 21.434230288261723 26.743295774932175 27.345251649850866 24.47722228695524 116-117 21.581622776153335 26.691412518223363 27.538105063824204 24.188859641799095 118-119 21.67837059967136 26.92181362947006 26.996542033695214 24.403273737163367 120-121 21.573886350640393 27.006224908211333 27.15043065611895 24.269458085029324 122-123 21.492289447909332 26.924816669380974 26.99427242345072 24.58862145925897 124-125 21.494083723236713 26.988530182883437 26.9014964024813 24.615889691398554 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 970.0 1 703.0 2 805.0 3 1243.0 4 909.0 5 647.5 6 1290.0 7 3035.5 8 5254.0 9 6033.5 10 4563.0 11 2710.0 12 2116.0 13 2151.0 14 2348.5 15 2689.0 16 3020.0 17 3462.0 18 3957.0 19 4359.5 20 4920.0 21 5677.0 22 6581.5 23 7532.0 24 8494.0 25 10071.0 26 12785.5 27 15814.5 28 18292.5 29 21134.0 30 24565.0 31 28342.5 32 32873.0 33 37370.0 34 41727.0 35 45074.5 36 49604.0 37 55502.5 38 57923.5 39 58392.5 40 58981.5 41 63985.5 42 70727.0 43 74275.5 44 75124.0 45 74898.5 46 73743.5 47 72141.5 48 79139.0 49 82955.5 50 79097.0 51 76169.0 52 73549.5 53 73345.5 54 72672.0 55 68366.5 56 61160.0 57 54735.0 58 49551.5 59 43463.5 60 38435.0 61 33429.5 62 28204.5 63 22714.5 64 18300.5 65 15232.0 66 11653.0 67 10027.0 68 7307.0 69 4250.0 70 3468.0 71 2997.5 72 2663.5 73 2390.5 74 1952.0 75 1728.0 76 1182.5 77 575.0 78 317.5 79 191.0 80 116.5 81 67.0 82 42.5 83 33.0 84 23.0 85 14.5 86 11.5 87 8.5 88 6.5 89 5.0 90 2.0 91 1.5 92 1.0 93 1.0 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10028010536292133 2 0.0 3 0.0 4 0.0978487944826675 5 0.0 6 0.0 7 0.0021101943488995335 8 0.005734223774183515 9 0.003302912893929705 10-11 7.56917538192224E-4 12-13 0.0 14-15 2.293689509673406E-5 16-17 3.899272166444791E-4 18-19 0.0019037622930289271 20-21 0.0012844661254171074 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.293689509673406E-5 52-53 0.0 54-55 0.0 56-57 0.007982039493663454 58-59 8.257282234824262E-4 60-61 0.0 62-63 0.0 64-65 2.293689509673406E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0013532768107073095 76-77 0.0 78-79 0.0 80-81 6.881068529020218E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0015367719714811819 90-91 0.0 92-93 0.002614806041027683 94-95 0.0 96-97 0.008050850178953656 98-99 0.0036699032154774497 100-101 0.0021101943488995335 102-103 0.0 104-105 0.0 106-107 0.0016973302371583205 108-109 0.0 110-111 1.6055826567713843E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010321602793530327 122-123 0.004082767327218663 124-125 0.09193107554771011 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2179894.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.51798135001846 #Duplication Level Percentage of deduplicated Percentage of total 1 73.16905300843716 31.110004312112434 2 12.309092612361058 10.46715540256035 3 5.152524938794709 6.572248777595354 4 2.7302491679015106 4.643387328069594 5 1.6365060216839429 3.479046625457539 6 1.0918844267584775 2.7854833015975524 7 0.7415653165332649 2.2070902208727325 8 0.5135574900115203 1.7468342225977687 9 0.38914332014650616 1.4891029585626072 >10 1.9840256167780803 15.011453486500667 >50 0.15329698758142302 4.5132084418888905 >100 0.1159653945177505 9.581690528171682 >500 0.010096032727248527 2.9459899552950475 >1k 0.00271398729227111 2.0784172612139553 >5k 1.0855949169084439E-4 0.3074076475090484 >10k+ 2.1711898338168877E-4 1.0614795299948327 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12446 0.5709451927479042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10551 0.48401436033128215 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6660 0.3055194426884977 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 3530 0.16193447938294248 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3396 0.15578739149701776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2912 0.13358447704337917 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2865 0.13142840890428617 No Hit GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG 2663 0.12216190328520561 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2458 0.11275777629554465 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2936895096734063E-5 0.0 14-15 0.0 0.0 0.0 9.174758038693625E-5 0.0 16-17 0.0 0.0 0.0 2.9817963625754283E-4 0.0 18-19 0.0 0.0 0.0 3.8992721664447903E-4 0.0 20-21 0.0 4.5873790193468126E-5 0.0 8.027913283856921E-4 0.0 22-23 0.0 4.5873790193468126E-5 0.0 0.0012615292303203734 0.0 24-25 0.0 4.5873790193468126E-5 0.0 0.0018578885028354588 0.0 26-27 0.0 4.5873790193468126E-5 0.0 0.0027524274116080875 0.0 28-29 0.0 4.5873790193468126E-5 0.0 0.004197451802702333 0.0 30-31 0.0 4.5873790193468126E-5 0.0 0.007110437479987559 0.0 32-33 0.0 4.5873790193468126E-5 0.0 0.01309696710023515 0.0 34-35 0.0 4.5873790193468126E-5 0.0 0.021514807600736548 0.0 36-37 0.0 4.5873790193468126E-5 0.0 0.03708895937141898 0.0 38-39 0.0 4.5873790193468126E-5 0.0 0.06254891292879379 0.0 40-41 0.0 4.5873790193468126E-5 0.0 0.09204576002319378 2.2936895096734063E-5 42-43 0.0 4.5873790193468126E-5 0.0 0.11780389321682613 4.5873790193468126E-5 44-45 0.0 4.5873790193468126E-5 0.0 0.1484705219611596 4.5873790193468126E-5 46-47 0.0 4.5873790193468126E-5 0.0 0.18895414180689518 4.5873790193468126E-5 48-49 0.0 4.5873790193468126E-5 0.0 0.23443800478371885 4.5873790193468126E-5 50-51 0.0 4.5873790193468126E-5 0.0 0.2852661643180815 4.5873790193468126E-5 52-53 0.0 4.5873790193468126E-5 0.0 0.33366301297219036 4.5873790193468126E-5 54-55 0.0 4.5873790193468126E-5 0.0 0.3862802503240983 4.5873790193468126E-5 56-57 0.0 4.5873790193468126E-5 0.0 0.4495172701057941 4.5873790193468126E-5 58-59 0.0 4.5873790193468126E-5 0.0 0.5197271059968971 4.5873790193468126E-5 60-61 0.0 4.5873790193468126E-5 0.0 0.6041807537430719 4.5873790193468126E-5 62-63 0.0 4.5873790193468126E-5 0.0 0.690423479306792 4.5873790193468126E-5 64-65 0.0 4.5873790193468126E-5 0.0 0.7744183891510321 4.5873790193468126E-5 66-67 0.0 4.5873790193468126E-5 0.0 0.8750654848355012 4.5873790193468126E-5 68-69 0.0 4.5873790193468126E-5 0.0 0.9906903730181376 4.5873790193468126E-5 70-71 0.0 4.5873790193468126E-5 0.0 1.126752034731964 4.5873790193468126E-5 72-73 0.0 4.5873790193468126E-5 0.0 1.2685708571150707 4.5873790193468126E-5 74-75 0.0 4.5873790193468126E-5 0.0 1.4106649222393384 4.5873790193468126E-5 76-77 0.0 4.5873790193468126E-5 0.0 1.5664752506314525 4.5873790193468126E-5 78-79 0.0 4.5873790193468126E-5 0.0 1.7480666491122963 4.5873790193468126E-5 80-81 0.0 4.5873790193468126E-5 0.0 1.955691423527933 4.5873790193468126E-5 82-83 0.0 4.5873790193468126E-5 0.0 2.178890349714252 4.5873790193468126E-5 84-85 0.0 4.5873790193468126E-5 0.0 2.4018828438447004 4.5873790193468126E-5 86-87 0.0 4.5873790193468126E-5 0.0 2.6337060425873915 4.5873790193468126E-5 88-89 0.0 4.5873790193468126E-5 0.0 2.893145263026551 4.5873790193468126E-5 90-91 0.0 4.5873790193468126E-5 0.0 3.187150384376488 4.5873790193468126E-5 92-93 0.0 4.5873790193468126E-5 0.0 3.512854294750112 4.5873790193468126E-5 94-95 0.0 9.174758038693625E-5 0.0 3.8437190065205007 4.5873790193468126E-5 96-97 0.0 9.174758038693625E-5 0.0 4.182382262623779 4.5873790193468126E-5 98-99 0.0 9.174758038693625E-5 0.0 4.542835569068955 4.5873790193468126E-5 100-101 0.0 9.174758038693625E-5 0.0 4.972420677335688 4.5873790193468126E-5 102-103 0.0 9.174758038693625E-5 0.0 5.432557729871269 4.5873790193468126E-5 104-105 0.0 9.174758038693625E-5 0.0 5.887189927583635 4.5873790193468126E-5 106-107 0.0 9.174758038693625E-5 0.0 6.34849676176915 4.5873790193468126E-5 108-109 0.0 9.174758038693625E-5 0.0 6.833612093065076 4.5873790193468126E-5 110-111 0.0 9.174758038693625E-5 0.0 7.36950971010517 4.5873790193468126E-5 112-113 0.0 9.174758038693625E-5 0.0 7.953047258261181 4.5873790193468126E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8240 0.0 57.241364 1 GTATCAA 14015 0.0 48.716156 1 ATCAACG 17905 0.0 38.090164 3 TCAACGC 18080 0.0 37.72148 4 TATCAAC 18125 0.0 37.36493 2 CAACGCA 18370 0.0 37.18313 5 AACGCAG 18695 0.0 36.695858 6 GTACATG 13325 0.0 32.80178 1 ACGCAGA 21405 0.0 31.716381 7 CGCAGAG 21380 0.0 31.558659 8 TACATGG 13565 0.0 31.351631 2 ACATGGG 13865 0.0 29.64223 3 GAGTACT 13040 0.0 29.040503 12-13 GCAGAGT 22340 0.0 28.842216 9 AGTACTT 13245 0.0 26.794264 12-13 GTACTTT 14245 0.0 26.771881 14-15 CATGGGG 4675 0.0 26.246252 4 CATGGGA 9345 0.0 25.176737 4 CAGAGTA 22165 0.0 24.78806 10-11 AGAGTAC 21560 0.0 23.89695 10-11 >>END_MODULE