FastQCFastQC Report
Thu 26 May 2016
SRR1364560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985453
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34130.17190031695537492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24220.12198727444064403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66250.049.308671
GGTATCA38200.048.1317831
ATCAACG80450.040.2345123
TCAACGC81900.039.4495284
CAACGCA82150.039.3294755
AACGCAG82250.038.7684256
TATCAAC85200.037.9215552
GTACATG104750.037.6045841
TACATGG108950.035.007142
ACGCAGA96750.032.2817967
ACATGGG112300.032.2143753
CGCAGAG97000.031.4044558
GCAGAGT104900.027.7930059
CATGGGA71800.026.7672624
GAGTACT40300.025.10546312-13
CATGGGG49450.024.6668364
GTATTAC3603.6379788E-1223.1399461
CAGAGTA103200.022.7485310-11
AGTACTT44150.022.10739112-13
GTACTTT46500.021.9500514-15
Produced by FastQC (version 0.11.5)