##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364560_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985453 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.6934578657868 31.0 28.0 31.0 12.0 34.0 2 27.600108892026153 31.0 27.0 33.0 12.0 34.0 3 27.78566604195617 31.0 27.0 34.0 15.0 34.0 4 31.06889057560164 35.0 32.0 37.0 16.0 37.0 5 31.162405254619475 35.0 32.0 37.0 16.0 37.0 6 31.24414579443583 35.0 32.0 37.0 13.0 37.0 7 30.950377571264593 35.0 32.0 37.0 11.0 37.0 8 30.904865539501564 35.0 32.0 37.0 10.0 37.0 9 31.926791518106953 37.0 31.0 39.0 10.0 39.0 10-11 31.821254645665245 37.0 31.0 39.0 9.0 39.0 12-13 31.740995379895672 37.0 31.0 39.0 8.0 39.0 14-15 32.38180581459244 38.0 31.0 40.0 8.0 41.0 16-17 32.490691796783906 38.0 31.0 40.0 8.0 41.0 18-19 32.29215750763176 38.0 31.0 40.0 7.0 41.0 20-21 32.01622199064899 38.0 30.0 40.0 2.0 41.0 22-23 31.9128541446209 38.0 30.0 40.0 2.0 41.0 24-25 31.732130148636106 38.0 30.0 40.0 2.0 41.0 26-27 31.637892964477125 37.0 29.5 40.0 2.0 41.0 28-29 31.697429251661962 37.5 30.0 40.0 2.0 41.0 30-31 31.636569588904898 37.0 30.0 40.0 2.0 41.0 32-33 31.620948468686997 37.0 30.0 40.0 2.0 41.0 34-35 31.11900180966258 37.0 29.0 40.0 2.0 41.0 36-37 30.971009386774703 37.0 29.0 40.0 2.0 41.0 38-39 30.75777215577503 37.0 28.0 40.0 2.0 41.0 40-41 30.95775346986305 37.0 29.0 40.0 2.0 41.0 42-43 30.822157965965452 37.0 29.0 40.0 2.0 41.0 44-45 30.65243171205765 36.5 28.5 40.0 2.0 41.0 46-47 30.59786985640053 36.0 28.5 40.0 2.0 41.0 48-49 30.42603828949867 36.0 28.0 40.0 2.0 41.0 50-51 30.259318654231553 36.0 28.0 40.0 2.0 41.0 52-53 30.18266587020695 36.0 28.0 40.0 2.0 41.0 54-55 30.198832709714104 36.0 28.0 40.0 2.0 41.0 56-57 30.065251103904245 36.0 28.0 40.0 2.0 41.0 58-59 29.85849173966848 35.5 28.0 40.0 2.0 41.0 60-61 29.65280618579236 35.0 28.0 39.5 2.0 41.0 62-63 29.3696277373476 35.0 27.0 39.0 2.0 41.0 64-65 29.090579580579345 35.0 26.5 39.0 2.0 40.5 66-67 28.849065427386094 34.5 26.0 38.5 2.0 40.0 68-69 28.819330903325337 35.0 27.0 38.0 2.0 40.0 70-71 28.593742083041 34.5 26.0 37.5 2.0 40.0 72-73 28.32326955107978 34.0 26.0 37.0 2.0 40.0 74-75 28.035514565189906 34.0 26.0 36.5 2.0 39.0 76-77 27.732671838618188 34.0 26.0 36.0 2.0 39.0 78-79 27.427452072650425 34.0 26.0 36.0 2.0 38.5 80-81 27.135315467049587 34.0 26.0 35.0 2.0 37.0 82-83 26.89316795713623 34.0 25.0 35.0 2.0 37.0 84-85 26.65629682495632 33.5 25.0 35.0 2.0 36.5 86-87 26.447412504854057 33.0 24.5 35.0 2.0 36.0 88-89 26.25356656642086 33.0 24.0 35.0 2.0 36.0 90-91 26.117890224548255 33.0 24.0 35.0 2.0 35.5 92-93 25.98826791669206 33.0 23.5 35.0 2.0 35.0 94-95 25.856880772297302 33.0 23.0 35.0 2.0 35.0 96-97 25.742662002072073 33.0 22.5 35.0 2.0 35.0 98-99 25.642465472615065 33.0 20.0 35.0 2.0 35.0 100-101 25.537628692293396 33.0 20.0 35.0 2.0 35.0 102-103 24.401261324241872 31.0 18.5 34.0 2.0 35.0 104-105 25.243543161182863 32.5 19.0 35.0 2.0 35.0 106-107 25.387683818252057 33.0 19.0 35.0 2.0 35.0 108-109 25.33428189939525 33.0 19.0 35.0 2.0 35.0 110-111 25.244024663389162 33.0 18.0 35.0 2.0 35.0 112-113 25.142315129091447 33.0 17.5 35.0 2.0 35.0 114-115 25.00490794795948 33.0 15.5 35.0 2.0 35.0 116-117 24.889959873137265 33.0 8.5 35.0 2.0 35.0 118-119 24.730068402525774 33.0 2.0 35.0 2.0 35.0 120-121 24.539967956934767 33.0 2.0 35.0 2.0 35.0 122-123 24.285832502708452 33.0 2.0 35.0 2.0 35.0 124-125 22.86247370247495 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9410092267794958 1101 2 0.8763952755332802 1101 3 0.7520767480420716 1101 4 0.8889284387196845 1101 5 0.934902624109931 1101 6 0.9671058121942018 1101 7 0.8573308653099083 1101 8 0.7778366143022666 1101 9 1.1298213589109096 1101 10-11 1.2817804921728424 1101 12-13 0.997167772490485 1101 14-15 1.3208439514037238 1101 16-17 1.110834177361248 1101 18-19 1.5354042865301878 1101 20-21 1.8057811359804887 1101 22-23 1.647520641701604 1101 24-25 1.8233837290015913 1101 26-27 1.84565257491575 1101 28-29 1.6204335510791736 1101 30-31 1.7661983449942404 1101 32-33 1.7132413094733039 1101 34-35 1.998432211609554 1101 36-37 1.9981983425968899 1101 38-39 1.7808555933869243 1101 40-41 1.5931169266042247 1101 42-43 1.661300110313114 1101 44-45 1.5788267931900322 1101 46-47 1.4040260949570644 1101 48-49 1.3944539397838227 1101 50-51 1.3187114649910754 1101 52-53 1.0005515586098888 1101 54-55 0.995419978749883 1101 56-57 0.927118660186327 1101 58-59 0.9050262751200044 1101 60-61 0.8877873237040461 1101 62-63 0.8862080671019044 1101 64-65 0.951853461898672 1101 66-67 1.0125362385384662 1101 68-69 0.8310585639560237 1101 70-71 0.7875430858695651 1101 72-73 0.6505499466370104 1101 74-75 0.7740109897050651 1101 76-77 0.8708400980536943 1101 78-79 0.9851374020391148 1101 80-81 0.9518580873956424 1101 82-83 0.8029772188258448 1101 84-85 0.9379486495174909 1101 86-87 0.97200094627091 1101 88-89 0.8884368628707762 1101 90-91 0.8975766850840721 1101 92-93 0.8383811653284852 1101 94-95 0.8366077574153294 1101 96-97 0.8118024006321996 1101 98-99 0.9541209967716107 1101 100-101 1.0782822896894366 1101 102-103 0.9632815549103562 1101 104-105 1.0718796048043195 1101 106-107 1.0124803504184712 1101 108-109 1.0512718631297453 1101 110-111 0.9509274319485996 1101 112-113 0.948929424084799 1101 114-115 1.140411004428909 1101 116-117 1.222963092860109 1101 118-119 1.1503677456552488 1101 120-121 1.2874771560662168 1101 122-123 1.3185489217863307 1101 124-125 1.6063156113713681 1102 1 -0.15423191878202758 1102 2 0.026818285863377866 1102 3 0.14054021400436412 1102 4 0.3030070853880744 1102 5 0.2578814439200592 1102 6 -0.40637566503078304 1102 7 -0.9906647223075389 1102 8 0.008596261914551206 1102 9 0.2768467687330798 1102 10-11 0.23989983186036312 1102 12-13 0.344360177427383 1102 14-15 0.5562000270962386 1102 16-17 0.5319966338638622 1102 18-19 0.6988588504824236 1102 20-21 1.01997939457776 1102 22-23 0.8424432224743938 1102 24-25 0.9160797945957455 1102 26-27 0.5601479204361972 1102 28-29 1.0774336631785602 1102 30-31 0.9715465219190982 1102 32-33 0.7643691093674221 1102 34-35 1.1101961237085618 1102 36-37 1.0785250887567805 1102 38-39 1.2285068286468004 1102 40-41 0.9056834561767957 1102 42-43 0.9667678756165685 1102 44-45 1.0741817337585147 1102 46-47 0.6100204918717864 1102 48-49 0.31127584211982295 1102 50-51 0.7182909038912584 1102 52-53 0.5176262262210791 1102 54-55 0.4276347046527498 1102 56-57 0.471291705841864 1102 58-59 0.6526896152295372 1102 60-61 0.5040626473321481 1102 62-63 0.4261881011650459 1102 64-65 0.5524816188152002 1102 66-67 0.5140142548126612 1102 68-69 0.3679906015837382 1102 70-71 0.36524315030316146 1102 72-73 0.4748615951762325 1102 74-75 0.15261371324914208 1102 76-77 0.4460925807253915 1102 78-79 0.29912801771908804 1102 80-81 -0.0956182398516674 1102 82-83 0.219398530708947 1102 84-85 -0.28712101224380504 1102 86-87 0.25272818868253566 1102 88-89 0.4908181034624377 1102 90-91 0.05330380974424287 1102 92-93 0.3584288216977072 1102 94-95 0.25485598313638747 1102 96-97 0.42883333535267454 1102 98-99 0.48010450403169713 1102 100-101 0.5033778803213202 1102 102-103 0.2107513116264279 1102 104-105 0.4914210947653004 1102 106-107 0.3735921549548209 1102 108-109 0.12221664429101864 1102 110-111 0.4064615384222918 1102 112-113 0.12719535491477174 1102 114-115 0.1857759303208546 1102 116-117 0.13350319052787896 1102 118-119 0.4930274260495331 1102 120-121 0.7738156321551379 1102 122-123 0.5801201218526515 1102 124-125 0.8153411662633268 1103 1 0.7352043386435803 1103 2 0.8113124884904295 1103 3 0.7017832567289055 1103 4 0.6751532117083272 1103 5 0.8259312766982063 1103 6 0.8793844693172623 1103 7 0.8817688240034549 1103 8 0.8377216302828501 1103 9 1.0766800223463946 1103 10-11 1.118927277735537 1103 12-13 1.0130383878064535 1103 14-15 1.406349371379271 1103 16-17 1.347595551510068 1103 18-19 1.2707800569902403 1103 20-21 1.737163710432597 1103 22-23 1.672956479929482 1103 24-25 1.6826843018356783 1103 26-27 1.6555276143683848 1103 28-29 1.665006183830485 1103 30-31 1.6457476417397139 1103 32-33 1.6938022715008216 1103 34-35 2.082832504375304 1103 36-37 1.9730715499962166 1103 38-39 2.063953178341592 1103 40-41 1.7742194780558975 1103 42-43 1.5792540514044404 1103 44-45 1.4107109602587826 1103 46-47 1.496837281002982 1103 48-49 1.4052369793235222 1103 50-51 1.33422814030407 1103 52-53 1.3443907805132689 1103 54-55 1.3510668749613401 1103 56-57 1.143402077020177 1103 58-59 1.1556606916019518 1103 60-61 1.0757782142270678 1103 62-63 1.0497244939086556 1103 64-65 1.159090662013309 1103 66-67 1.1842155058622517 1103 68-69 1.2698199145239606 1103 70-71 1.2168314964091316 1103 72-73 1.1192864556503501 1103 74-75 1.1918447589824375 1103 76-77 1.2281696263997333 1103 78-79 1.327012154893307 1103 80-81 1.298477359508162 1103 82-83 1.2542965239516057 1103 84-85 1.1954319524375343 1103 86-87 1.2628502934432007 1103 88-89 1.1591134437876747 1103 90-91 1.197623710809001 1103 92-93 1.232067202179966 1103 94-95 1.2117900557896988 1103 96-97 1.282078922778279 1103 98-99 1.2341031841344332 1103 100-101 1.1867083291529532 1103 102-103 1.2391620288498508 1103 104-105 1.236468508365757 1103 106-107 1.2599438181870255 1103 108-109 1.2864639747797888 1103 110-111 1.3083571790526882 1103 112-113 1.388611562834722 1103 114-115 1.4441926526964401 1103 116-117 1.5617045357200006 1103 118-119 1.5467780548550571 1103 120-121 1.4797815344922505 1103 122-123 1.4694017335346885 1103 124-125 1.4702053870627374 1104 1 0.6657996928755274 1104 2 0.6442880458342408 1104 3 0.5715992512262709 1104 4 0.5981822219088748 1104 5 0.47321659986545583 1104 6 0.6317938939235574 1104 7 0.5830284951942062 1104 8 0.6330985650829533 1104 9 0.8312241895113246 1104 10-11 0.8763372867717223 1104 12-13 0.8996742744989845 1104 14-15 0.9232335508621858 1104 16-17 0.9866940234506885 1104 18-19 1.2232419678961506 1104 20-21 1.4024785832741387 1104 22-23 1.0646315009464828 1104 24-25 1.530213287310353 1104 26-27 1.6833631108415723 1104 28-29 1.464920092812271 1104 30-31 1.475457472299997 1104 32-33 1.5345513083154074 1104 34-35 1.7940697424495582 1104 36-37 1.8763680717392468 1104 38-39 1.9324603870594927 1104 40-41 1.5099914200265019 1104 42-43 1.5591493260640092 1104 44-45 1.3424208897225895 1104 46-47 1.3956006498366875 1104 48-49 1.273597968178894 1104 50-51 1.1192982044564914 1104 52-53 1.2013689857740353 1104 54-55 1.1092358561202182 1104 56-57 1.009051760753696 1104 58-59 0.9902925457282521 1104 60-61 0.9957224461288483 1104 62-63 1.0184788490352261 1104 64-65 1.0586590830186182 1104 66-67 1.0675957809039502 1104 68-69 1.1127215639649322 1104 70-71 1.0113875319964158 1104 72-73 1.012626202727077 1104 74-75 1.0565568823061362 1104 76-77 1.0666526487114645 1104 78-79 1.0446755367642915 1104 80-81 1.0364241673970689 1104 82-83 0.9485800129081277 1104 84-85 0.9884572312780229 1104 86-87 1.0462710290156494 1104 88-89 1.0363115745111529 1104 90-91 1.0175290556451166 1104 92-93 1.0738921519414717 1104 94-95 1.1127941512945299 1104 96-97 0.9905331506789601 1104 98-99 1.073410140198792 1104 100-101 1.1532681500476691 1104 102-103 1.0964083975172123 1104 104-105 1.1538012377882048 1104 106-107 1.117558040513149 1104 108-109 1.1763558102942433 1104 110-111 1.110163067408596 1104 112-113 1.1339648956585542 1104 114-115 1.0576221771952916 1104 116-117 1.101661332864385 1104 118-119 0.9503498205975767 1104 120-121 0.9966757167174869 1104 122-123 0.9900612177078791 1104 124-125 1.2512478301594996 1105 1 0.4270359288337069 1105 2 0.5750850546898967 1105 3 0.6924494828955119 1105 4 0.7552150778294973 1105 5 0.7457329611583603 1105 6 0.7389384177516014 1105 7 0.8841651744906436 1105 8 0.5647678058522949 1105 9 0.5798513964802865 1105 10-11 0.8561398401334301 1105 12-13 0.8677783645444279 1105 14-15 0.997653266636803 1105 16-17 0.9097926134350232 1105 18-19 0.9387532513548535 1105 20-21 1.2953535041065933 1105 22-23 1.6225788114721524 1105 24-25 1.6581835980915827 1105 26-27 1.3342820099404484 1105 28-29 1.1856564343271714 1105 30-31 1.2132040028170117 1105 32-33 1.2311196035186924 1105 34-35 1.787874118053729 1105 36-37 1.66273069799065 1105 38-39 1.8114573759149195 1105 40-41 1.466898163434763 1105 42-43 0.9552820608721717 1105 44-45 1.1964176007971545 1105 46-47 1.077782674081405 1105 48-49 1.0400075949525238 1105 50-51 0.9615542295234327 1105 52-53 0.8532112284656215 1105 54-55 0.7490540763226683 1105 56-57 0.6631195837295074 1105 58-59 0.6861030547339055 1105 60-61 0.6768316806758996 1105 62-63 0.5857168183459969 1105 64-65 0.6909055524238354 1105 66-67 0.5265364358001143 1105 68-69 0.38278012017110896 1105 70-71 0.5356216901537287 1105 72-73 0.6238596608766116 1105 74-75 0.6583791247754966 1105 76-77 0.7599753522970616 1105 78-79 0.8858482164607331 1105 80-81 0.6883291885639693 1105 82-83 0.6327120588197488 1105 84-85 0.6105391432992668 1105 86-87 0.6728668800806616 1105 88-89 0.8455711218394555 1105 90-91 0.6479155043834375 1105 92-93 0.569527659002663 1105 94-95 0.6666814167085917 1105 96-97 0.7688756878182623 1105 98-99 0.5755046079151036 1105 100-101 0.5237189328341465 1105 102-103 0.4687519791125645 1105 104-105 0.5923388882065446 1105 106-107 0.6070095899748083 1105 108-109 0.578465278177223 1105 110-111 0.6589258314393085 1105 112-113 0.7024506566114539 1105 114-115 0.7295907546239313 1105 116-117 0.8077108445256265 1105 118-119 0.8312357193297863 1105 120-121 0.6878389518645456 1105 122-123 0.9128570265501992 1105 124-125 0.9932010740844284 1106 1 0.5902707689239257 1106 2 0.8842607673990379 1106 3 0.5633348667586979 1106 4 0.8254292077310055 1106 5 0.6134908786207305 1106 6 0.6274002368846396 1106 7 0.29068809244138194 1106 8 0.6158347504960169 1106 9 0.8677675902154789 1106 10-11 0.9968038639972434 1106 12-13 1.039344769737525 1106 14-15 1.1975196108680564 1106 16-17 1.0705561970936586 1106 18-19 1.077416873297075 1106 20-21 1.4057527761569872 1106 22-23 1.3714145860861962 1106 24-25 1.55680892521422 1106 26-27 1.7699554699801219 1106 28-29 1.5657685569783766 1106 30-31 1.3847093338914966 1106 32-33 1.2606408565260807 1106 34-35 1.7266600005017665 1106 36-37 1.6093229530289896 1106 38-39 1.7527982671704336 1106 40-41 1.419343317416761 1106 42-43 1.2223927498533484 1106 44-45 1.2670341013624267 1106 46-47 0.9724964508981628 1106 48-49 1.1603134483005455 1106 50-51 1.3064031216007521 1106 52-53 1.1965478624958052 1106 54-55 1.0408865917792447 1106 56-57 1.070583256417141 1106 58-59 1.017922221453901 1106 60-61 1.1438176953763524 1106 62-63 1.0042758845406858 1106 64-65 1.0469173114370385 1106 66-67 0.9183042284085765 1106 68-69 0.8761107478311736 1106 70-71 0.9417461107074168 1106 72-73 0.9046072238059679 1106 74-75 1.0020906797966553 1106 76-77 0.8061890937492109 1106 78-79 0.7996653937811296 1106 80-81 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-2.618146770876688 2216 72-73 -2.7669047674125835 2216 74-75 -2.5845126775540415 2216 76-77 -2.682289874967605 2216 78-79 -2.988026148360955 2216 80-81 -2.923032558963616 2216 82-83 -2.668498770053798 2216 84-85 -2.5786026027137616 2216 86-87 -2.6292927980669027 2216 88-89 -2.795217750210398 2216 90-91 -2.674335027867471 2216 92-93 -2.5692022815366897 2216 94-95 -2.623088342237587 2216 96-97 -2.5266990808526977 2216 98-99 -2.504717082615386 2216 100-101 -2.602210720898416 2216 102-103 -2.4775053456280673 2216 104-105 -2.7672579239436352 2216 106-107 -2.7074764056674923 2216 108-109 -2.7854914381534144 2216 110-111 -2.7701395733541645 2216 112-113 -2.7690946012510516 2216 114-115 -2.8802986637200085 2216 116-117 -2.8927879519786295 2216 118-119 -2.92374150962965 2216 120-121 -2.939364653158794 2216 122-123 -2.9696892176899397 2216 124-125 -3.1309441371911397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 154923.0 3 25586.0 4 15982.0 5 12699.0 6 11800.0 7 12134.0 8 12792.0 9 13284.0 10 13930.0 11 14794.0 12 15630.0 13 17504.0 14 19547.0 15 17274.0 16 13322.0 17 12072.0 18 11213.0 19 10595.0 20 10175.0 21 11037.0 22 13280.0 23 17433.0 24 23360.0 25 29516.0 26 35862.0 27 39764.0 28 45445.0 29 52791.0 30 59345.0 31 67533.0 32 82169.0 33 101636.0 34 130221.0 35 175471.0 36 258903.0 37 322991.0 38 103179.0 39 261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.61042477363456 18.89677232485801 13.331016157265733 23.16178674424169 2 15.02621965071023 20.782454215719902 40.414024176175104 23.777301957394766 3 19.302662364973948 26.178570619035746 28.633960705497756 25.884806310492554 4 12.122651512365842 16.886746216000166 37.5880423137039 33.402559957930094 5 13.58131535823833 38.80315366802057 33.22010742943615 14.395423544304945 6 31.113175036730095 36.79668222837483 18.125553721639 13.964589013256075 7 28.413228185904632 33.25295827884889 20.547974185957017 17.78583934928947 8 26.39176046977692 35.358731735276535 19.732020853679234 18.517486941267308 9 27.12739273129484 16.865569853348564 19.498002405575132 36.50903500978146 10-11 24.797691005528712 27.393244765804077 27.091147461058007 20.717916767609207 12-13 25.680856829420872 24.79041647736488 27.397643557913256 22.131083135300997 14-15 22.70909008932082 25.969132541962097 25.523951586760035 25.797825781957044 16-17 22.350761355463383 29.708091440749808 25.745440621327525 22.195706582459287 18-19 22.09088605822434 27.987111404150767 28.185643530693525 21.736359006931373 20-21 23.162695086617855 27.1042708505434 28.071443107040245 21.661590955798502 22-23 22.78375751104306 27.14482147264293 27.795568673472985 22.275852342841027 24-25 22.533685539837567 27.20639089582455 28.11990132979112 22.140022234546763 26-27 22.480589108742553 27.609044184415115 27.731208431096217 22.179158275746115 28-29 22.14925496419814 27.42767792453215 28.179072513594583 22.243994597675126 30-31 22.23811033632071 27.20123762756487 28.088232590573792 22.472419445540634 32-33 22.27757770496857 27.413783506808592 27.716088078601707 22.592550709621133 34-35 22.591731883760485 27.226437661961832 27.858153356150183 22.323677098127494 36-37 22.833497800969475 26.885824199460707 27.735439325042954 22.54523867452686 38-39 22.542512968073282 26.96419910216963 27.973842745207268 22.519445184549824 40-41 22.588927813118023 27.051521970386073 27.86402711860308 22.49552309789283 42-43 22.70892483357818 27.043863429209996 27.537682443168755 22.70952929404307 44-45 22.490261083071335 27.37989508719309 27.385965380688006 22.743878449047568 46-47 22.64038088858052 27.51053651546384 26.91641177974244 22.932670816213204 48-49 22.889160772838945 27.39537275012463 26.93825001618457 22.77721646085186 50-51 22.73737277833505 27.545963245521538 26.78501946872701 22.931644507416397 52-53 23.0575339733552 27.46209555199745 26.72883719735496 22.751533277292385 54-55 22.719540049227184 27.36914646347103 26.842845786723167 23.068467700578616 56-57 22.785332307817843 27.77615757075729 26.672846958938585 22.76566316248628 58-59 22.940643179128156 27.212168526026847 26.786421214334283 23.060767080510715 60-61 22.860183700468152 27.18103066383963 27.153957372283948 22.804828263408268 62-63 22.709441344483285 27.32921335606181 27.058768999559923 22.90257629989498 64-65 22.614954290312873 27.198869568393253 26.9675693579486 23.218606783345276 66-67 22.68546185746309 27.59268087588329 26.675611960603433 23.046245306050185 68-69 22.556405181788023 27.413330189741465 26.99811409676772 23.032150531702797 70-71 22.495492046862566 27.322049179502162 27.060663449818172 23.121795323817103 72-73 22.634614047795832 27.312544768924347 26.87082471536787 23.182016467911954 74-75 22.58356153482354 27.542429863612988 26.727275840828263 23.14673276073521 76-77 22.707570071497596 27.440379003496428 26.80080682552839 23.05124409947759 78-79 22.75008782743263 27.450561654857708 26.72535260038455 23.07399791732511 80-81 22.51690553476039 27.812466237410092 26.79585498021654 22.87477324761298 82-83 22.611011019884547 27.59738411845205 26.79520764214563 22.99639721951777 84-85 22.682581758419868 27.474586404211028 26.649837593738052 23.192994243631052 86-87 22.767063706434865 27.148957282651264 26.91128748928068 23.17269152163319 88-89 22.78336612794837 27.213208320865334 26.79634896694444 23.20707658424186 90-91 22.599532235333236 27.517383742092143 26.88782842079676 22.99525560177786 92-93 22.667371163787774 27.284035156689747 26.847283879012192 23.201309800510288 94-95 22.596860131672546 27.242579564759158 26.748480042560825 23.412080261007475 96-97 22.667056915745995 27.295261546645765 26.67527934966099 23.362402187947247 98-99 22.4969880549685 27.18570001027486 26.717413071639967 23.599898863116668 100-101 22.247897567043502 27.26175091971604 26.970328060387068 23.52002345285339 102-103 22.59761374356381 26.893912875298483 26.985025583582186 23.52344779755552 104-105 22.4355582641938 26.956018066729037 27.103896283779104 23.504527385298054 106-107 22.416085207823006 27.124737118987795 26.9753504429597 23.483827230229497 108-109 22.506022555054187 27.23602623683361 26.54235582509382 23.715595383018385 110-111 22.17854570546431 27.183809048921393 27.055902247875775 23.581742997738527 112-113 22.309719746576725 27.156220771783566 26.988551227352147 23.54550825428756 114-115 22.032553779918235 27.318224103013268 26.779530918133045 23.86969119893546 116-117 21.924165417161724 27.26156197099604 27.034057215154427 23.780215396687808 118-119 22.012482793599244 27.42260834177389 26.686176907738435 23.878731956888426 120-121 21.90814892117819 27.412409158010792 26.975078231516935 23.70436368929408 122-123 21.961310592595243 27.362017635270135 26.853896818509426 23.822774953625192 124-125 21.947814679567866 27.447281047928108 26.55195874424145 24.05294552826258 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1150.0 1 1145.0 2 1338.5 3 1378.5 4 1028.0 5 779.0 6 729.5 7 847.0 8 1091.0 9 1250.5 10 1202.5 11 1089.5 12 1089.5 13 1232.0 14 1413.0 15 1533.5 16 1667.5 17 1809.5 18 2022.0 19 2221.0 20 2539.0 21 3129.0 22 3823.0 23 4694.5 24 5797.0 25 7480.5 26 9921.0 27 12904.0 28 15731.0 29 18664.5 30 22372.0 31 26695.0 32 30686.5 33 34414.0 34 38788.0 35 42139.0 36 45342.5 37 49762.0 38 52856.5 39 54122.5 40 55775.0 41 60096.0 42 64743.5 43 67835.5 44 70692.5 45 72505.5 46 71496.0 47 70909.0 48 74912.5 49 76569.0 50 73878.5 51 70783.5 52 68648.0 53 68212.0 54 66556.0 55 62262.0 56 57158.5 57 51804.5 58 47309.5 59 43167.5 60 37483.0 61 31265.0 62 25272.5 63 19618.5 64 15309.0 65 12232.0 66 9567.0 67 7947.5 68 6171.5 69 4277.5 70 3103.5 71 2369.0 72 1997.5 73 1657.5 74 1294.5 75 1014.5 76 701.0 77 457.5 78 307.0 79 191.5 80 135.5 81 114.0 82 79.5 83 41.5 84 39.0 85 38.0 86 28.0 87 22.0 88 18.5 89 15.5 90 12.5 91 9.0 92 7.0 93 6.5 94 5.0 95 4.0 96 3.5 97 3.5 98 2.5 99 2.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014001842400701502 2 0.005540297352795559 3 0.009468871839323318 4 0.00936813916018158 5 0.016923090095811888 6 0.0012087921497008491 7 0.008914842104043762 8 0.0 9 0.0032738120721064667 10-11 0.0 12-13 0.004381871542665578 14-15 0.021027946770837688 16-17 0.03377063068226747 18-19 0.00664835682335467 20-21 0.009116307462327238 22-23 0.0021657526015473544 24-25 0.012793050251000655 26-27 0.021179045789550294 28-29 5.792129050649902E-4 30-31 0.0020902030921910518 32-33 0.010249550102671784 34-35 0.011307243233660027 36-37 0.013095248288425867 38-39 0.0 40-41 0.027323739217196278 42-43 0.010652480819238733 44-45 0.018937743678646635 46-47 0.004809985429017962 48-49 0.027071907519341937 50-51 0.0045077873915927505 52-53 0.0 54-55 0.018685911980792294 56-57 0.005540297352795559 58-59 1.5109901871260614E-4 60-61 0.005112183466443174 62-63 0.028784363064751472 64-65 0.018660728811006857 66-67 0.0025938664878997386 68-69 0.02329443205152678 70-71 0.003676742788673416 72-73 0.013044881948854996 74-75 0.0 76-77 0.007126837049277923 78-79 0.0012843416590571522 80-81 0.004155223014596669 82-83 0.002115386261976486 84-85 0.0 86-87 0.00682463901185271 88-89 0.018685911980792294 90-91 0.024201026163802417 92-93 0.00566621320172273 94-95 0.026643793632989552 96-97 0.01994507047006401 98-99 0.001259158489271718 100-101 0.009745886706963096 102-103 0.0 104-105 0.0017880050547658393 106-107 7.554950935630307E-5 108-109 0.0 110-111 0.0011584258101299805 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.02364699642852286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1985453.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.52432721827311 #Duplication Level Percentage of deduplicated Percentage of total 1 78.75865510663542 43.73021337411941 2 10.971989527838307 12.1842467355832 3 4.089943330960649 6.8127405543735895 4 1.9256278025592328 4.276767528396122 5 1.124622725966537 3.122196011683614 6 0.6790060951971186 2.262081396775603 7 0.4377048815346082 1.7012288347154136 8 0.3191914580584283 1.4178312770011097 9 0.2511356427250269 1.254972384255215 >10 1.2842346463973882 12.846065759197867 >50 0.1020843237236669 3.914026103575871 >100 0.0535654378991369 5.246964579709996 >500 0.001492680336349675 0.5487696729410646 >1k 7.463401681748373E-4 0.6818957876719061 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.17190031695537492 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2422 0.12198727444064403 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.5183169785434358E-5 0.0 14-15 0.0 0.0 0.0 5.0366339570868716E-5 0.0 16-17 0.0 0.0 0.0 1.0073267914173743E-4 0.0 18-19 0.0 0.0 0.0 1.0073267914173743E-4 0.0 20-21 0.0 0.0 0.0 1.0073267914173743E-4 0.0 22-23 0.0 0.0 0.0 3.021980374252123E-4 0.0 24-25 7.554950935630307E-5 0.0 0.0 6.043960748504246E-4 0.0 26-27 1.0073267914173743E-4 0.0 0.0 0.001133242640344546 0.0 28-29 1.0073267914173743E-4 0.0 0.0 0.0018131882245512737 0.0 30-31 1.5109901871260614E-4 0.0 0.0 0.004004123995884063 0.0 32-33 1.5109901871260614E-4 0.0 0.0 0.0076808667845574795 0.0 34-35 2.266485280689092E-4 0.0 0.0 0.012616768062502613 0.0 36-37 3.021980374252123E-4 0.0 0.0 0.021833808203971586 0.0 38-39 3.273812072106466E-4 0.0 0.0 0.03744737347094089 0.0 40-41 3.52564376996081E-4 0.0 0.0 0.058903434128130955 0.0 42-43 3.7774754678151536E-4 0.0 0.0 0.07879813825862411 0.0 44-45 4.532970561378184E-4 0.0 0.0 0.10289843174328478 0.0 46-47 5.288465654941215E-4 0.0 0.0 0.13357153254194382 0.0 48-49 5.540297352795558E-4 0.0 0.0 0.16668740080979 0.0 50-51 5.540297352795558E-4 0.0 0.0 0.20937287359610124 0.0 52-53 5.540297352795558E-4 0.0 0.0 0.2492126481966584 0.0 54-55 5.540297352795558E-4 0.0 0.0 0.29051304664477073 0.0 56-57 5.540297352795558E-4 0.0 0.0 0.3415089654602753 0.0 58-59 5.540297352795558E-4 0.0 0.0 0.39958135498548697 0.0 60-61 5.792129050649901E-4 0.0 0.0 0.4676010965759451 0.0 62-63 6.043960748504246E-4 0.0 0.0 0.5368548134858897 0.0 64-65 6.043960748504246E-4 0.0 0.0 0.6044716243597809 0.0 66-67 6.043960748504246E-4 0.0 0.0 0.683043114090336 0.0 68-69 6.547624144212933E-4 0.0 0.0 0.7772533522576459 0.0 70-71 6.547624144212933E-4 0.0 0.0 0.8861201952400788 0.0 72-73 7.051287539921621E-4 0.0 0.0 1.0027182713466396 0.0 74-75 7.554950935630307E-4 0.0 0.0 1.1140782481378304 0.0 76-77 7.554950935630307E-4 0.0 0.0 1.2410517901959905 0.0 78-79 7.554950935630307E-4 0.0 0.0 1.393284051548941 0.0 80-81 8.562277727047681E-4 0.0 0.0 1.5666953586914423 0.0 82-83 8.562277727047681E-4 0.0 0.0 1.7505576812948984 0.0 84-85 8.814109424902024E-4 0.0 0.0 1.937970830838101 0.0 86-87 9.065941122756369E-4 0.0 0.0 2.130823545054957 0.0 88-89 9.065941122756369E-4 0.0 0.0 2.3485572310198224 0.0 90-91 9.065941122756369E-4 0.0 0.0 2.599280869403607 0.0 92-93 9.065941122756369E-4 0.0 0.0 2.8787385045125724 0.0 94-95 9.065941122756369E-4 0.0 0.0 3.1639379023326164 0.0 96-97 9.065941122756369E-4 0.0 0.0 3.448507720908024 0.0 98-99 9.569604518465056E-4 0.0 0.0 3.760527194549556 0.0 100-101 9.8214362163194E-4 0.0 0.0 4.119211081803497 0.0 102-103 0.0010073267914173744 0.0 0.0 4.4965808810382315 0.0 104-105 0.0011080594705591116 0.0 0.0 4.887474042447744 0.0 106-107 0.0011080594705591116 0.0 0.0 5.280155208912022 0.0 108-109 0.001133242640344546 0.0 0.0 5.691900034903873 0.0 110-111 0.0012591584892717178 0.0 0.0 6.160281809743167 0.0 112-113 0.0012591584892717178 0.0 0.0 6.668402626503876 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6625 0.0 49.30867 1 GGTATCA 3820 0.0 48.131783 1 ATCAACG 8045 0.0 40.234512 3 TCAACGC 8190 0.0 39.449528 4 CAACGCA 8215 0.0 39.329475 5 AACGCAG 8225 0.0 38.768425 6 TATCAAC 8520 0.0 37.921555 2 GTACATG 10475 0.0 37.604584 1 TACATGG 10895 0.0 35.00714 2 ACGCAGA 9675 0.0 32.281796 7 ACATGGG 11230 0.0 32.214375 3 CGCAGAG 9700 0.0 31.404455 8 GCAGAGT 10490 0.0 27.793005 9 CATGGGA 7180 0.0 26.767262 4 GAGTACT 4030 0.0 25.105463 12-13 CATGGGG 4945 0.0 24.666836 4 GTATTAC 360 3.6379788E-12 23.139946 1 CAGAGTA 10320 0.0 22.74853 10-11 AGTACTT 4415 0.0 22.107391 12-13 GTACTTT 4650 0.0 21.95005 14-15 >>END_MODULE