FastQCFastQC Report
Thu 26 May 2016
SRR1364560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985453
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75340.3794600023269249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66580.3353390888628439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.19426297172484064No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54650.057.613681
GTATCAA94150.049.5299951
ATCAACG119400.038.9174963
CAACGCA122600.037.9995655
TCAACGC122850.037.9215554
TATCAAC122200.037.879512
AACGCAG125600.037.2814186
GTACATG105200.032.5937271
ACGCAGA145850.031.9012457
CGCAGAG146800.031.5732088
TACATGG107050.030.8859442
ACATGGG109500.029.7596473
GCAGAGT157650.028.5321929
GTGGTAT31250.027.6694071
GAGTACT89550.027.47370512-13
CATGGGG45100.025.3617524
TGGTATC31050.025.1341532
CAGAGTA153550.024.91292610-11
GTACTTT100100.024.84560414-15
AGTACTT94750.024.30183412-13
Produced by FastQC (version 0.11.5)