##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364560_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985453 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.978108774168916 31.0 30.0 34.0 26.0 34.0 2 29.811805668530052 31.0 30.0 34.0 26.0 34.0 3 30.083655971710236 31.0 30.0 34.0 26.0 34.0 4 33.639964280191975 35.0 35.0 37.0 28.0 37.0 5 33.0497166137904 35.0 33.0 37.0 26.0 37.0 6 33.082428040351495 35.0 33.0 37.0 26.0 37.0 7 32.85464425498866 35.0 33.0 37.0 25.0 37.0 8 32.77279089457166 35.0 33.0 37.0 25.0 37.0 9 33.96441013713243 38.0 33.0 39.0 25.0 39.0 10-11 33.902105715924776 38.0 33.0 39.0 24.5 39.0 12-13 33.78143803958089 38.0 33.0 39.0 23.0 39.0 14-15 34.709680360099185 39.0 33.0 41.0 23.0 41.0 16-17 34.71848187793919 39.0 33.0 41.0 23.5 41.0 18-19 34.74774975786382 39.0 33.5 41.0 23.0 41.0 20-21 34.772846549376894 39.0 34.0 41.0 23.0 41.0 22-23 34.72913586974862 39.0 34.0 41.0 23.5 41.0 24-25 34.69327453231077 39.0 34.0 41.0 23.5 41.0 26-27 34.56378065862047 39.0 33.0 41.0 23.0 41.0 28-29 34.40483934900499 38.5 33.0 41.0 22.5 41.0 30-31 34.221168166660206 38.0 33.0 40.0 21.5 41.0 32-33 34.00145306889662 38.0 33.0 40.0 19.5 41.0 34-35 33.81070138653496 38.0 33.0 40.0 18.0 41.0 36-37 33.589930106630575 38.0 33.0 40.0 16.5 41.0 38-39 33.409568748290695 38.0 32.0 40.0 16.0 41.0 40-41 33.42207823604991 38.0 32.5 40.0 15.0 41.0 42-43 33.271774753670826 38.0 32.0 40.0 14.0 41.0 44-45 33.07818089876719 38.0 32.0 40.0 9.0 41.0 46-47 32.799534161725305 38.0 31.5 40.0 7.5 41.0 48-49 32.48337835244652 38.0 31.0 40.0 2.0 41.0 50-51 32.295307922171915 38.0 31.0 40.0 2.0 41.0 52-53 32.103330574936805 37.0 31.0 40.0 2.0 41.0 54-55 31.907299492861327 37.0 30.0 40.0 2.0 41.0 56-57 31.69902334630938 37.0 30.0 40.0 2.0 41.0 58-59 31.480807654474823 36.5 30.0 40.0 2.0 41.0 60-61 31.24237944690708 36.0 30.0 40.0 2.0 41.0 62-63 31.071662738931618 36.0 29.5 39.5 2.0 41.0 64-65 30.93878172890519 35.5 30.0 39.0 2.0 41.0 66-67 30.64372060179717 35.0 29.0 39.0 2.0 41.0 68-69 30.362843139575702 35.0 29.0 39.0 2.0 40.5 70-71 30.05486606834813 35.0 29.0 38.0 2.0 40.0 72-73 29.770334276359097 35.0 29.0 37.0 2.0 40.0 74-75 29.426369448181347 35.0 29.0 37.0 2.0 39.0 76-77 29.120983725124695 34.5 29.0 36.0 2.0 39.0 78-79 28.82936161168257 34.0 28.5 36.0 2.0 38.5 80-81 28.54874983190234 34.0 28.0 35.5 2.0 37.0 82-83 28.27022598872902 34.0 28.0 35.0 2.0 37.0 84-85 28.04897270295494 34.0 27.5 35.0 2.0 36.5 86-87 27.829472921293025 34.0 27.0 35.0 2.0 36.0 88-89 27.646944047529708 34.0 27.0 35.0 2.0 36.0 90-91 27.50770730911283 34.0 27.0 35.0 2.0 35.5 92-93 27.39828190342456 34.0 26.5 35.0 2.0 35.0 94-95 27.288129963287975 34.0 26.0 35.0 2.0 35.0 96-97 27.173720556467465 34.0 26.0 35.0 2.0 35.0 98-99 27.076265718705002 34.0 26.0 35.0 2.0 35.0 100-101 26.97152060512135 34.0 25.0 35.0 2.0 35.0 102-103 25.701391571596005 31.5 23.5 34.0 2.0 35.0 104-105 26.63439552585732 33.0 25.0 35.0 2.0 35.0 106-107 26.801803417154673 34.0 25.0 35.0 2.0 35.0 108-109 26.800519579159015 34.0 25.0 35.0 2.0 35.0 110-111 26.722514962580327 34.0 25.0 35.0 2.0 35.0 112-113 26.63377098324664 34.0 25.0 35.0 2.0 35.0 114-115 26.52331029744849 34.0 25.0 35.0 2.0 35.0 116-117 26.39663240580361 34.0 25.0 35.0 2.0 35.0 118-119 26.259288938091206 34.0 25.0 35.0 2.0 35.0 120-121 26.06249153215916 34.0 24.0 35.0 2.0 35.0 122-123 25.755022657297857 33.0 23.0 35.0 2.0 35.0 124-125 23.95490600885541 31.0 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5631801744862877 1101 2 0.7969881382756938 1101 3 0.5277813303049221 1101 4 0.4543652287278874 1101 5 0.5726491193330929 1101 6 0.6679886952953566 1101 7 0.7462726832966382 1101 8 0.8196290323801776 1101 9 0.9233119025479866 1101 10-11 0.9648500360590972 1101 12-13 0.8258677568973596 1101 14-15 0.8963326132933886 1101 16-17 1.0176128524564376 1101 18-19 1.1744797793186663 1101 20-21 1.143040641790165 1101 22-23 1.0730431125634965 1101 24-25 1.0207185637313074 1101 26-27 0.9561419632262798 1101 28-29 1.117821463597359 1101 30-31 1.1351308318843039 1101 32-33 1.1890396217827117 1101 34-35 1.204780050003059 1101 36-37 1.3691836261842028 1101 38-39 1.3296545998386478 1101 40-41 1.0920326075410998 1101 42-43 1.0455856643388373 1101 44-45 1.0900637771721762 1101 46-47 1.103551715094774 1101 48-49 1.1843257709564838 1101 50-51 1.1160641005469856 1101 52-53 1.1333799722778437 1101 54-55 1.0876857163247244 1101 56-57 1.0313261263647675 1101 58-59 1.1180947049172616 1101 60-61 1.09540360937687 1101 62-63 1.166148860416996 1101 64-65 1.0720317631786003 1101 66-67 1.3015134360433258 1101 68-69 1.2543579809458656 1101 70-71 1.1636011253279719 1101 72-73 1.0977526099246653 1101 74-75 1.1103194014572466 1101 76-77 1.234083053568476 1101 78-79 1.1021954114861288 1101 80-81 0.9140957015669926 1101 82-83 1.1727262244035899 1101 84-85 1.1792182648474423 1101 86-87 1.1983922862560306 1101 88-89 1.168016956582818 1101 90-91 1.1576967938607439 1101 92-93 1.1347479908111104 1101 94-95 1.1124175144068111 1101 96-97 1.1147235572564291 1101 98-99 1.1429774638584398 1101 100-101 1.0781672004805145 1101 102-103 1.0587885150055634 1101 104-105 1.161196907955187 1101 106-107 1.092966131409291 1101 108-109 1.0984550093857592 1101 110-111 0.9237254281975176 1101 112-113 0.9716297831648539 1101 114-115 1.099756592678073 1101 116-117 1.1161164449890109 1101 118-119 1.1623500944880618 1101 120-121 1.1670938944437488 1101 122-123 1.2135557230776222 1101 124-125 1.4737234825080314 1102 1 0.38120095149517397 1102 2 -0.4510647236498926 1102 3 0.15319864146455586 1102 4 0.21565924186706553 1102 5 0.4948992460336541 1102 6 0.16688889669744356 1102 7 0.15866206757898738 1102 8 0.36605489888959397 1102 9 0.7797797074159263 1102 10-11 0.8152611770424443 1102 12-13 0.8559255822823104 1102 14-15 1.01435489752091 1102 16-17 0.9328736059903946 1102 18-19 1.0025903253274535 1102 20-21 0.7195994591133825 1102 22-23 0.9337463195479501 1102 24-25 0.9999469714113332 1102 26-27 1.0329266278418743 1102 28-29 0.683381588432745 1102 30-31 0.8641536455223715 1102 32-33 0.9902237068727402 1102 34-35 1.098152456409494 1102 36-37 0.6723663067348653 1102 38-39 -0.08911997489019541 1102 40-41 -0.34160808849246393 1102 42-43 0.014912116591091262 1102 44-45 -0.20113521996221806 1102 46-47 0.027277503040416207 1102 48-49 -0.2972926380235208 1102 50-51 -0.40759993853519205 1102 52-53 -0.631462951990077 1102 54-55 -0.4738356513254658 1102 56-57 -0.40567613823122883 1102 58-59 -0.306448631386548 1102 60-61 -0.4788449243820061 1102 62-63 -0.3932074112139432 1102 64-65 -0.5256481327856406 1102 66-67 -0.6311080033881495 1102 68-69 -0.3529039397405249 1102 70-71 -0.9009628221906745 1102 72-73 -0.6668419765228002 1102 74-75 -0.5772127446902076 1102 76-77 -0.8324088385372796 1102 78-79 -0.6477663192907102 1102 80-81 -0.6167133781064678 1102 82-83 -0.9787004993364192 1102 84-85 -0.8962300384953821 1102 86-87 -0.704482191634014 1102 88-89 -0.7716902944576347 1102 90-91 -0.739241362132617 1102 92-93 -0.638813015747175 1102 94-95 -0.5064273814914806 1102 96-97 -0.7431602088684564 1102 98-99 -0.8404961193089413 1102 100-101 -0.5923406756788658 1102 102-103 -0.7982505021748487 1102 104-105 -0.6247300032451406 1102 106-107 -0.37148293778900054 1102 108-109 -0.4218334487107356 1102 110-111 -0.4196765573746326 1102 112-113 -0.2539861276002675 1102 114-115 -0.38618812254238577 1102 116-117 -0.33383153241778274 1102 118-119 -0.0789837516136096 1102 120-121 -0.051068584667138595 1102 122-123 -0.17583185631040976 1102 124-125 0.11114143329357518 1103 1 0.2506064842230167 1103 2 -0.18486365042507202 1103 3 0.1478864829095201 1103 4 0.17350193255560953 1103 5 0.25776087458527996 1103 6 0.38283141079742933 1103 7 0.5872654203842913 1103 8 0.5331117657311708 1103 9 0.7300969392071934 1103 10-11 0.6848093499641976 1103 12-13 0.7204723145381067 1103 14-15 0.7897769460813393 1103 16-17 0.6637079940687158 1103 18-19 0.7749300381642215 1103 20-21 0.8336179471843863 1103 22-23 0.8697393853326574 1103 24-25 0.8191505256245932 1103 26-27 0.8445433878744666 1103 28-29 0.7777427125135432 1103 30-31 0.78330494587793 1103 32-33 0.8037259325352366 1103 34-35 0.7687914939875782 1103 36-37 0.9200480469832684 1103 38-39 0.9529059865760274 1103 40-41 0.8183447023580186 1103 42-43 0.8001077523717228 1103 44-45 0.8988656596523086 1103 46-47 0.8826251314570044 1103 48-49 0.6193824285656788 1103 50-51 0.6735724354826118 1103 52-53 0.7183072071107901 1103 54-55 0.8173794006383552 1103 56-57 0.910773390438024 1103 58-59 0.7162639666904695 1103 60-61 0.764877837284299 1103 62-63 0.6595546021809184 1103 64-65 0.6580618578286348 1103 66-67 0.7009473134299853 1103 68-69 0.7351744781324854 1103 70-71 0.6534302818216062 1103 72-73 0.5988826784543413 1103 74-75 0.6420062146639935 1103 76-77 0.6300877262113822 1103 78-79 0.48490522693850124 1103 80-81 0.5548751676957693 1103 82-83 0.6318859468021643 1103 84-85 0.6808893332791648 1103 86-87 0.6171640759000319 1103 88-89 0.6878552824125954 1103 90-91 0.5731323418623546 1103 92-93 0.6212835326454105 1103 94-95 0.51945622696309 1103 96-97 0.5076212216605391 1103 98-99 0.6344488928963052 1103 100-101 0.6335337562565613 1103 102-103 0.5654766433000837 1103 104-105 0.46917051187614334 1103 106-107 0.6183357617698597 1103 108-109 0.6755574719556385 1103 110-111 0.5422953291940793 1103 112-113 0.42133198380552983 1103 114-115 0.4954760273173058 1103 116-117 0.48526135677836635 1103 118-119 0.431600031908026 1103 120-121 0.632558555767833 1103 122-123 0.5427988311107228 1103 124-125 0.846891202454195 1104 1 0.399738830619107 1104 2 0.574577140083786 1104 3 0.3278430103170358 1104 4 0.021285369812567012 1104 5 0.38296059226068735 1104 6 0.4515203547326223 1104 7 0.4270826286975833 1104 8 0.3382641895417393 1104 9 0.3724543945095462 1104 10-11 0.4715041292070481 1104 12-13 0.5091766334749863 1104 14-15 0.5566961913575739 1104 16-17 0.5583427029102381 1104 18-19 0.49206158883654183 1104 20-21 0.4352332214843102 1104 22-23 0.48702165148203136 1104 24-25 0.5025376564784523 1104 26-27 0.39864338832403234 1104 28-29 0.5483280190470197 1104 30-31 0.4531413954213974 1104 32-33 0.593152921507496 1104 34-35 0.64641906828885 1104 36-37 0.6863141918589406 1104 38-39 0.8029625189287231 1104 40-41 0.767632484626418 1104 42-43 0.7073019719105673 1104 44-45 0.6784444430491092 1104 46-47 0.7280834368800626 1104 48-49 0.7968825521950791 1104 50-51 0.5583652326090132 1104 52-53 0.668237974647063 1104 54-55 0.3562863594000838 1104 56-57 0.6186327361462887 1104 58-59 0.7307205143282545 1104 60-61 0.6442988246111625 1104 62-63 0.6149719300775551 1104 64-65 0.6887150930976311 1104 66-67 0.7455821346618734 1104 68-69 0.4265977447359113 1104 70-71 0.026554261879056185 1104 72-73 -0.03733107003400704 1104 74-75 0.4901803208234341 1104 76-77 0.6289651712637365 1104 78-79 0.6470631668681008 1104 80-81 0.7068727940113 1104 82-83 0.6755717438071898 1104 84-85 0.6456583261266466 1104 86-87 0.4957804606167393 1104 88-89 0.6163475881683311 1104 90-91 0.7420143724415276 1104 92-93 0.6837017686561815 1104 94-95 0.645825591148327 1104 96-97 0.5873678038695935 1104 98-99 0.5627860999508236 1104 100-101 0.6442534367082828 1104 102-103 0.4837763794075087 1104 104-105 0.5300433803929039 1104 106-107 0.5146580119148467 1104 108-109 0.5351329061196104 1104 110-111 0.640766145912373 1104 112-113 0.5308928072222869 1104 114-115 0.5344693018779694 1104 116-117 0.5587481329282298 1104 118-119 0.4947246413515103 1104 120-121 0.5267432535951038 1104 122-123 0.5765682015898186 1104 124-125 0.8821100714474923 1105 1 0.04671824150144843 1105 2 -0.2677499968844046 1105 3 0.035590874182190646 1105 4 -0.11171944135200107 1105 5 -0.20082367200891582 1105 6 0.2912274625893758 1105 7 0.25442071023181967 1105 8 0.40944831380978997 1105 9 0.47136327127484634 1105 10-11 0.5149551675519177 1105 12-13 0.4691345218959668 1105 14-15 0.4084906557405148 1105 16-17 0.4571238004779872 1105 18-19 0.45991234273380144 1105 20-21 0.46911470905639163 1105 22-23 0.4709264726467097 1105 24-25 0.4829857725686253 1105 26-27 0.4651280159310005 1105 28-29 0.5157626572096703 1105 30-31 0.542775169129996 1105 32-33 0.7189120966094364 1105 34-35 0.781651681984556 1105 36-37 0.6562221908367079 1105 38-39 0.735017875099345 1105 40-41 0.600693963087295 1105 42-43 0.4497996663293904 1105 44-45 0.5908890257666997 1105 46-47 0.5492558943518091 1105 48-49 0.5475464484605865 1105 50-51 0.3251065297345406 1105 52-53 0.42906476161136453 1105 54-55 0.47768993055087705 1105 56-57 0.4029592286628727 1105 58-59 0.15502578438680814 1105 60-61 0.2698446640093799 1105 62-63 0.4360838313209001 1105 64-65 0.1452540546416472 1105 66-67 0.3293997326351814 1105 68-69 0.47853665516825217 1105 70-71 0.5456172514678315 1105 72-73 0.5272790923011321 1105 74-75 0.47716128727861573 1105 76-77 0.5471081506301729 1105 78-79 0.3252634802049137 1105 80-81 0.39880825996092284 1105 82-83 0.3949591814453832 1105 84-85 0.43230428885956584 1105 86-87 0.5458560170118112 1105 88-89 0.4704076332799403 1105 90-91 0.4202485750437752 1105 92-93 0.42081829217311295 1105 94-95 0.40496124821117974 1105 96-97 0.46900648874386874 1105 98-99 0.39038862886780734 1105 100-101 0.39564234102943985 1105 102-103 0.08188191391344546 1105 104-105 0.24471076558607763 1105 106-107 0.27060641134150387 1105 108-109 0.2891085058484997 1105 110-111 0.14200115963474857 1105 112-113 0.1914945916865598 1105 114-115 0.21039347881326975 1105 116-117 -0.08891406331839136 1105 118-119 0.20196527735857828 1105 120-121 0.18302221225020077 1105 122-123 0.18036824381251293 1105 124-125 0.4409535030411753 1106 1 -0.0492117971975361 1106 2 -0.49170856528011697 1106 3 0.03232688916331128 1106 4 -0.07651953026873315 1106 5 0.24705989647100068 1106 6 0.46952055324290143 1106 7 0.5583006007923856 1106 8 0.46275322073350367 1106 9 0.6837814134315892 1106 10-11 0.6361563068536782 1106 12-13 0.5934162554578748 1106 14-15 0.6784875459002748 1106 16-17 0.6828143365717807 1106 18-19 0.6268565935149084 1106 20-21 0.637708466248597 1106 22-23 0.5703656909877992 1106 24-25 0.6372344654814412 1106 26-27 0.5845775966639195 1106 28-29 0.6660355821771944 1106 30-31 0.6919573485105133 1106 32-33 0.6811458375900585 1106 34-35 0.7821980371283104 1106 36-37 0.959078079493203 1106 38-39 0.7599063081777118 1106 40-41 0.7060257111879906 1106 42-43 0.5001286133285063 1106 44-45 0.47655603036184857 1106 46-47 0.3923915114530203 1106 48-49 0.7356209970566141 1106 50-51 0.8594520206346132 1106 52-53 0.5998683016648769 1106 54-55 0.6777851343240009 1106 56-57 0.19852905355753236 1106 58-59 0.6536704217087035 1106 60-61 0.6922821051385313 1106 62-63 0.6406913346805396 1106 64-65 0.624752292585967 1106 66-67 0.4311486528919204 1106 68-69 0.557845103043622 1106 70-71 0.6525436627683376 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2216 56-57 -1.2022220338046488 2216 58-59 -1.3883501481905896 2216 60-61 -1.1636118467060967 2216 62-63 -1.437567136699947 2216 64-65 -1.3710751922700481 2216 66-67 -1.0854210466702483 2216 68-69 -1.0886921221356687 2216 70-71 -1.236238863929728 2216 72-73 -1.1073199006159307 2216 74-75 -1.077991217823417 2216 76-77 -1.168886025044742 2216 78-79 -1.2738851100611015 2216 80-81 -1.12289065809113 2216 82-83 -0.9973486067201947 2216 84-85 -0.9955098350329159 2216 86-87 -1.183385670432049 2216 88-89 -1.1155503368718307 2216 90-91 -1.1336922384637482 2216 92-93 -1.095434891506894 2216 94-95 -1.03665046792295 2216 96-97 -1.0162828247315439 2216 98-99 -1.0626455128350685 2216 100-101 -1.167887615997472 2216 102-103 -1.0088823705889212 2216 104-105 -0.9776561860660848 2216 106-107 -1.0846663954061242 2216 108-109 -1.0301994769085852 2216 110-111 -1.0477592052387052 2216 112-113 -1.0076924855202556 2216 114-115 -1.167957832666385 2216 116-117 -1.0919573115203391 2216 118-119 -1.0679954975949073 2216 120-121 -1.2631648486607752 2216 122-123 -1.249276717753272 2216 124-125 -1.5878763310000643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66723.0 3 22276.0 4 10734.0 5 8715.0 6 9256.0 7 11282.0 8 12482.0 9 13269.0 10 14542.0 11 15911.0 12 17705.0 13 20347.0 14 23610.0 15 20844.0 16 14159.0 17 12287.0 18 11585.0 19 10514.0 20 10504.0 21 10828.0 22 12153.0 23 15168.0 24 19780.0 25 25824.0 26 32132.0 27 35877.0 28 40719.0 29 48321.0 30 56374.0 31 65342.0 32 79419.0 33 98243.0 34 125234.0 35 172477.0 36 273923.0 37 401711.0 38 144730.0 39 453.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8939819412865 19.539558262289958 14.37381839447853 23.192641401945018 2 15.574480987462309 20.955721439893065 39.25804337851362 24.211754194131014 3 19.016063336679338 25.90456686710791 29.673429690856445 25.40594010535631 4 12.752466242463385 17.55432237130341 36.78882047388357 32.90439091234964 5 13.25848559497505 38.62881669825476 33.563121363235496 14.549576343534701 6 31.141961053724266 37.32860964223278 17.289152651812962 14.240276652229994 7 27.778771139935966 33.66312397344415 20.619361025807883 17.938743860811996 8 26.508464912081877 35.19848003816023 19.362186214465932 18.930868835291967 9 26.953569266321676 16.059054098841223 19.559516985784988 37.42785964905212 10-11 25.0438883338752 27.05238873164667 26.82321998210469 21.080502952373443 12-13 26.16055378797685 24.426188884853985 27.64749404795782 21.765763279211345 14-15 22.97272080064449 25.35024167059315 26.142505189628245 25.534532339134113 16-17 22.108536455456928 29.04006082268269 26.529513431130127 22.321889290730258 18-19 21.830346035017524 26.755459588618148 29.768772734644706 21.64542164171963 20-21 22.896093243770395 25.88636809500461 29.69483275448841 21.522705906736586 22-23 22.443694209835236 25.929674487383984 29.615609133029086 22.01102216975169 24-25 22.196370299372486 26.10159998750915 29.806245728304827 21.89578398481354 26-27 22.21107727052718 26.516568259233537 29.479771115206454 21.792583355032832 28-29 21.857354467720967 26.280022745438952 29.94986534559116 21.912757441248925 30-31 21.927086664856837 26.002101283686898 29.949160216837168 22.1216518346191 32-33 21.939376051712127 26.21646042490051 29.64522454069676 22.1989389826906 34-35 22.279046645778067 26.16337430299282 29.48465665014483 22.072922401084284 36-37 22.595825738509046 25.839644655401063 29.381531569873477 22.18299803621642 38-39 22.301233018359035 25.941107646466577 29.558065590069372 22.19959374510502 40-41 22.238552108763088 25.951281647059893 29.640616020626027 22.169550223551 42-43 22.493506519670824 25.939596656279452 29.198147727495943 22.368749096553785 44-45 22.318533856001626 26.332403738592653 28.87852293657921 22.47053946882651 46-47 22.536645289513274 26.55741536062551 28.277199208442603 22.628740141418607 48-49 22.82328012801109 26.568067841444748 27.981397696142896 22.627254334401268 50-51 22.721923591725314 26.74896528625087 27.72040630536364 22.808704816660182 52-53 22.9416913923422 26.72989489048595 27.694133278400447 22.634280438771405 54-55 22.612471813737216 26.681115090611563 27.697155258774696 23.00925783687652 56-57 22.671791407822912 27.053258723871952 27.51072775454837 22.764222113756762 58-59 22.848517174842968 26.540407054783515 27.683155261346016 22.9279205090275 60-61 22.753573114045007 26.49211540137188 28.040074481742955 22.714237002840157 62-63 22.644580355213648 26.63291450364224 27.982933869499803 22.739571271644305 64-65 22.53555248319646 26.456789284014416 27.917217550725404 23.09044068206372 66-67 22.58459404478474 26.900435316272915 27.645152013167774 22.86981862577457 68-69 22.461731015977467 26.708848486679987 27.892533314194118 22.93688718314843 70-71 22.44049594727249 26.691415007053802 27.875099536478576 22.99298950919513 72-73 22.623199844065812 26.71604414710396 27.593123584391066 23.067632424439157 74-75 22.578132496031706 26.909571776931447 27.409514988097012 23.102780738939842 76-77 22.686409600227254 26.900082751895916 27.468189879085532 22.945317768791305 78-79 22.703005309115852 26.80589769689839 27.443988852921724 23.04710814106403 80-81 22.444766285921652 27.184272377615244 27.482592732779153 22.88836860368395 82-83 22.56553038525717 26.978427593098402 27.46763584935025 22.98840617229418 84-85 22.617558814033874 26.848003956779635 27.27254183302249 23.261895396163997 86-87 22.756066247853767 26.488967505148697 27.50475584161398 23.250210405383555 88-89 22.714779384322014 26.627375251898716 27.37565483592649 23.282190527852784 90-91 22.504315766808823 27.014849259803498 27.457065832599874 23.023769140787806 92-93 22.604690777430214 26.632682022548025 27.426423757552925 23.336203442468832 94-95 22.471068315392003 26.612793150983684 27.37622094302912 23.539917590595195 96-97 22.628307856750858 26.607898382801803 27.248748208353135 23.5150455520942 98-99 22.509575721676605 26.52635454687174 27.216380710898957 23.747689020552702 100-101 22.233230373262785 26.60970913885003 27.46203216232518 23.69502832556201 102-103 22.593030406662862 26.253580417164248 27.407095509186064 23.74629366698683 104-105 22.430447862528098 26.24675577815239 27.544091952818828 23.778704406500683 106-107 22.41838868106801 26.459076355244264 27.416658565933506 23.705876397754224 108-109 22.51333134386242 26.431480984813287 27.03247748309264 24.02271018823165 110-111 22.231906562263827 26.402068447437998 27.47671120483739 23.889313785460782 112-113 22.33268679742104 26.331925258366727 27.40200850888941 23.93337943532282 114-115 22.113064373722267 26.525004621111652 27.159167202648465 24.202763802517612 116-117 22.030035462939694 26.41966342189918 27.38672232482965 24.163578790331474 118-119 21.99749880757691 26.577511530114283 27.042669859221046 24.38231980308776 120-121 21.91932923279011 26.592049673924212 27.286912444067475 24.201708649218205 122-123 22.00153323246101 26.516887719432564 27.104165113622397 24.37741393448403 124-125 21.937847248653856 26.576164191993563 26.888067274676867 24.597921284675714 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 381.0 1 300.5 2 381.5 3 569.5 4 426.5 5 317.5 6 666.5 7 1554.0 8 2658.0 9 3213.0 10 2564.5 11 1656.0 12 1445.0 13 1510.0 14 1650.0 15 1803.5 16 2045.5 17 2342.5 18 2697.0 19 3018.0 20 3403.5 21 3986.0 22 4644.0 23 5484.5 24 6647.0 25 8202.0 26 10415.0 27 13234.0 28 16191.5 29 19297.5 30 22855.5 31 27103.5 32 30794.0 33 34198.0 34 38527.5 35 41742.5 36 44711.0 37 49176.0 38 52147.5 39 53445.0 40 55104.0 41 59499.5 42 64281.0 43 67252.5 44 69698.0 45 71337.0 46 70778.5 47 70355.0 48 75226.5 49 77482.5 50 74454.0 51 71073.0 52 68464.0 53 68064.0 54 66953.0 55 62512.5 56 57306.0 57 51847.0 58 47223.5 59 43202.0 60 37361.0 61 30766.0 62 24702.5 63 19111.5 64 14749.0 65 11654.5 66 8933.0 67 7506.5 68 5787.5 69 3870.0 70 2765.0 71 2103.5 72 1751.0 73 1448.0 74 1167.5 75 975.0 76 660.0 77 328.0 78 183.5 79 106.5 80 62.5 81 38.5 82 27.0 83 21.5 84 16.0 85 12.0 86 6.0 87 3.5 88 2.0 89 1.0 90 2.0 91 1.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09685447099478053 2 0.0 3 0.0 4 0.09922168895461136 5 0.0 6 0.0 7 0.002619049657685173 8 0.00705128753992162 9 0.004381871542665578 10-11 8.310446029193338E-4 12-13 0.0 14-15 1.259158489271718E-4 16-17 4.7848022592325275E-4 18-19 0.0023672179598308294 20-21 0.0016872723756241018 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.5183169785434355E-5 52-53 0.0 54-55 0.0 56-57 0.009166673801898107 58-59 0.0010073267914173744 60-61 0.0 62-63 0.0 64-65 4.029307165669497E-4 66-67 0.0 68-69 2.5183169785434355E-5 70-71 0.0 72-73 0.0 74-75 0.0013850743381988897 76-77 0.0 78-79 0.0 80-81 1.762821884980405E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0016117228662677987 90-91 2.5183169785434355E-5 92-93 0.002342034790045395 94-95 0.0 96-97 0.009997718404817441 98-99 0.004709252749876225 100-101 0.0027953318461832136 102-103 0.0 104-105 0.0 106-107 0.0018131882245512737 108-109 2.5183169785434355E-5 110-111 1.259158489271718E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.001259158489271718 122-123 0.005288465654941215 124-125 0.09186820337726453 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1985453.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.91663722954146 #Duplication Level Percentage of deduplicated Percentage of total 1 75.74738911883195 37.8105494372965 2 12.06361717274005 12.043504041754632 3 4.696295080667533 7.032697735936842 4 2.3218496279064644 4.635957023110112 5 1.3217170795060527 3.298783598889632 6 0.8020108910952619 2.402021202296608 7 0.5727933719356824 2.0014343268079484 8 0.40248023458527726 1.6072367889483223 9 0.2859362637868378 1.2845679075196248 >10 1.5749606045377254 14.29342546787126 >50 0.1323839238128023 4.573242132677542 >100 0.07481162891880394 6.710648614749315 >500 0.002638649826084066 0.8608933343455614 >1k 9.133787859521766E-4 0.7260906297031264 >5k 2.0297306354492812E-4 0.7189477580929862 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7534 0.3794600023269249 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6658 0.3353390888628439 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3857 0.19426297172484064 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 5.0366339570868716E-5 0.0 0.0 0.0 0.0 14-15 5.0366339570868716E-5 0.0 0.0 0.0 0.0 16-17 7.554950935630307E-5 0.0 0.0 5.0366339570868716E-5 0.0 18-19 2.0146535828347487E-4 0.0 0.0 5.0366339570868716E-5 0.0 20-21 2.518316978543436E-4 0.0 0.0 5.0366339570868716E-5 0.0 22-23 2.518316978543436E-4 0.0 0.0 3.021980374252123E-4 0.0 24-25 2.518316978543436E-4 0.0 0.0 7.05128753992162E-4 0.0 26-27 2.518316978543436E-4 0.0 0.0 0.0011080594705591119 0.0 28-29 3.021980374252123E-4 0.0 0.0 0.0017628218849804048 0.0 30-31 3.021980374252123E-4 0.0 0.0 0.004079673505240366 0.0 32-33 3.021980374252123E-4 0.0 0.0 0.008083797501124429 0.0 34-35 3.021980374252123E-4 0.0 0.0 0.013170797797782168 0.0 36-37 3.021980374252123E-4 0.0 0.0 0.022815951825603527 0.0 38-39 3.021980374252123E-4 0.0 0.0 0.03915982901635043 0.0 40-41 3.021980374252123E-4 0.0 0.0 0.0607669886922531 0.0 42-43 3.273812072106466E-4 0.0 0.0 0.08066169282274624 0.0 44-45 4.0293071656694973E-4 0.0 0.0 0.10423313974191281 0.0 46-47 4.532970561378184E-4 0.0 0.0 0.13447812665421946 0.0 48-49 4.532970561378184E-4 0.0 0.0 0.1674177127335676 0.0 50-51 4.532970561378184E-4 0.0 0.0 0.20949878944502842 0.0 52-53 4.532970561378184E-4 0.0 0.0 0.248784534310306 0.0 54-55 4.532970561378184E-4 0.0 0.0 0.2901604822677747 0.0 56-57 5.540297352795558E-4 0.0 0.0 0.3409801188947812 0.0 58-59 5.540297352795558E-4 0.0 0.0 0.3984481123451424 0.0 60-61 5.540297352795558E-4 0.0 0.0 0.46692115099173837 0.0 62-63 5.540297352795558E-4 0.0 0.0 0.535948219373614 0.0 64-65 5.540297352795558E-4 0.0 0.0 0.602910267833084 0.0 66-67 5.540297352795558E-4 0.0 0.0 0.6814313912240683 0.0 68-69 5.540297352795558E-4 0.0 0.0 0.7755408967122364 0.0 70-71 5.540297352795558E-4 0.0 0.0 0.882947115847114 0.0 72-73 5.540297352795558E-4 0.0 0.0 0.9983112166341888 5.0366339570868716E-5 74-75 5.540297352795558E-4 0.0 0.0 1.1083364854267514 5.0366339570868716E-5 76-77 5.540297352795558E-4 0.0 0.0 1.2345041660517775 5.0366339570868716E-5 78-79 5.540297352795558E-4 0.0 0.0 1.3864594125370884 5.0366339570868716E-5 80-81 6.547624144212933E-4 0.0 0.0 1.5576042343989003 5.0366339570868716E-5 82-83 6.547624144212933E-4 0.0 0.0 1.7398800173058744 5.0366339570868716E-5 84-85 6.799455842067276E-4 0.0 0.0 1.9245230181726791 5.0366339570868716E-5 86-87 7.05128753992162E-4 0.0 0.0 2.1153610788067008 5.0366339570868716E-5 88-89 7.05128753992162E-4 0.0 0.0 2.332968848922639 5.0366339570868716E-5 90-91 7.554950935630307E-4 0.0 0.0 2.582131130779726 5.0366339570868716E-5 92-93 7.554950935630307E-4 0.0 0.0 2.859876310343282 5.0366339570868716E-5 94-95 7.554950935630307E-4 0.0 0.0 3.1453275398611806 5.0366339570868716E-5 96-97 7.554950935630307E-4 0.0 0.0 3.4302247396437995 5.0366339570868716E-5 98-99 8.058614331338995E-4 0.0 0.0 3.7424204954738287 5.0366339570868716E-5 100-101 8.310446029193338E-4 0.0 0.0 4.1006510856716325 5.0366339570868716E-5 102-103 8.562277727047681E-4 0.0 0.0 4.477290572982589 5.0366339570868716E-5 104-105 0.0011080594705591116 0.0 0.0 4.867176407600684 5.0366339570868716E-5 106-107 0.0011584258101299805 0.0 0.0 5.2605375196491675 1.0073267914173743E-4 108-109 0.001183608979915415 0.0 0.0 5.6735918704698625 1.7628218849804052E-4 110-111 0.0012339753194862834 0.0 0.0 6.144315680099201 2.0146535828347487E-4 112-113 0.0012591584892717178 0.0 0.0 6.652612779048409 2.0146535828347487E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5465 0.0 57.61368 1 GTATCAA 9415 0.0 49.529995 1 ATCAACG 11940 0.0 38.917496 3 CAACGCA 12260 0.0 37.999565 5 TCAACGC 12285 0.0 37.921555 4 TATCAAC 12220 0.0 37.87951 2 AACGCAG 12560 0.0 37.281418 6 GTACATG 10520 0.0 32.593727 1 ACGCAGA 14585 0.0 31.901245 7 CGCAGAG 14680 0.0 31.573208 8 TACATGG 10705 0.0 30.885944 2 ACATGGG 10950 0.0 29.759647 3 GCAGAGT 15765 0.0 28.532192 9 GTGGTAT 3125 0.0 27.669407 1 GAGTACT 8955 0.0 27.473705 12-13 CATGGGG 4510 0.0 25.361752 4 TGGTATC 3105 0.0 25.134153 2 CAGAGTA 15355 0.0 24.912926 10-11 GTACTTT 10010 0.0 24.845604 14-15 AGTACTT 9475 0.0 24.301834 12-13 >>END_MODULE