FastQCFastQC Report
Thu 26 May 2016
SRR1364559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007215
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24600.12255787247504628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30400.055.388461
GTATCAA55600.055.3250961
TCAACGC67500.044.778174
CAACGCA67900.044.6896325
ATCAACG68050.044.416263
AACGCAG69850.043.009716
TATCAAC71250.042.588442
ACGCAGA82300.036.0696757
CGCAGAG83550.034.74948
GTACATG94150.034.1255651
TACATGG99500.031.8122942
ACATGGG102900.030.182943
GCAGAGT93050.030.0522569
CAGAGTA89650.024.42601610-11
AGAGTAC86650.023.69221310-11
CATGGGA60100.023.6608264
GAGTACT38250.023.57137912-13
CATGGGG49100.023.3874154
GAGTACA76250.021.6925891
GTGTAGC12700.021.550661
Produced by FastQC (version 0.11.5)