##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364559_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2007215 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.819397523434212 31.0 30.0 33.0 23.0 34.0 2 28.82997785488849 31.0 29.0 34.0 20.0 34.0 3 29.06815263935353 31.0 30.0 34.0 22.0 34.0 4 32.4418604882885 35.0 33.0 37.0 24.0 37.0 5 32.59607764987806 35.0 33.0 37.0 25.0 37.0 6 32.59355524943766 35.0 33.0 37.0 25.0 37.0 7 32.37564834858249 35.0 33.0 37.0 23.0 37.0 8 32.36690688341807 35.0 33.0 37.0 23.0 37.0 9 33.53094162807671 38.0 33.0 39.0 19.0 39.0 10-11 33.44357754400998 38.0 33.0 39.0 18.5 39.0 12-13 33.3761076915029 38.0 33.0 39.0 18.0 39.0 14-15 34.14735167881866 39.0 33.0 41.0 17.0 41.0 16-17 34.28678293057794 39.0 33.0 41.0 17.0 41.0 18-19 34.07551981227721 39.0 33.0 41.0 17.5 41.0 20-21 33.78048514982201 39.0 32.5 41.0 16.5 41.0 22-23 33.7077784392803 38.5 32.0 41.0 16.5 41.0 24-25 33.52853207055547 38.0 32.0 41.0 15.5 41.0 26-27 33.44858448148305 38.0 32.0 40.5 13.5 41.0 28-29 33.52222557125171 38.0 32.0 40.5 14.5 41.0 30-31 33.47554073679203 38.0 32.0 40.0 14.5 41.0 32-33 33.47535042334778 38.0 32.0 40.0 15.5 41.0 34-35 32.99436433067708 38.0 31.5 40.0 9.0 41.0 36-37 32.87703011386424 38.0 31.0 40.0 9.0 41.0 38-39 32.67953831552674 38.0 31.0 40.0 9.0 41.0 40-41 32.906822388234445 38.0 31.5 40.0 9.0 41.0 42-43 32.81645214887294 38.0 31.5 40.0 8.5 41.0 44-45 32.66839850240258 38.0 31.0 40.0 8.0 41.0 46-47 32.64515535206742 38.0 31.0 40.0 6.0 41.0 48-49 32.5063144705475 38.0 31.0 40.0 2.0 41.0 50-51 32.35861404981529 38.0 31.0 40.0 2.0 41.0 52-53 32.29303437847963 37.5 31.0 40.0 2.0 41.0 54-55 32.31790565534833 38.0 31.0 40.0 2.0 41.0 56-57 32.19094765632979 37.0 31.0 40.0 2.0 41.0 58-59 31.988059575082886 37.0 31.0 40.0 2.0 41.0 60-61 31.77519249308121 36.5 31.0 40.0 2.0 41.0 62-63 31.497250668214416 36.0 30.0 40.0 2.0 41.0 64-65 31.209246891837694 36.0 30.0 39.0 2.0 41.0 66-67 30.954112289914136 35.0 30.0 39.0 2.0 41.0 68-69 30.911284790119645 35.0 30.0 39.0 2.0 41.0 70-71 30.668625184646388 35.0 30.0 38.5 2.0 40.0 72-73 30.376004314435672 35.0 30.0 37.5 2.0 40.0 74-75 30.070622728506912 35.0 30.0 37.0 2.0 39.5 76-77 29.763489710868043 35.0 29.5 36.5 2.0 39.0 78-79 29.449926141444738 35.0 29.0 36.0 2.0 39.0 80-81 29.15161131219127 34.0 29.0 36.0 2.0 37.5 82-83 28.900348741913547 34.0 29.0 35.0 2.0 37.0 84-85 28.658977239608113 34.0 29.0 35.0 2.0 37.0 86-87 28.46040907426459 34.0 29.0 35.0 2.0 36.0 88-89 28.26580485897126 34.0 29.0 35.0 2.0 36.0 90-91 28.126314570188047 34.0 29.0 35.0 2.0 36.0 92-93 27.999816412292653 34.0 28.5 35.0 2.0 35.5 94-95 27.87939259122715 34.0 28.0 35.0 2.0 35.0 96-97 27.766631875509102 34.0 28.0 35.0 2.0 35.0 98-99 27.676085023278524 34.0 28.0 35.0 2.0 35.0 100-101 27.58993979219964 34.0 27.5 35.0 2.0 35.0 102-103 26.401251734368266 32.0 25.0 34.0 2.0 35.0 104-105 27.29564097518203 33.5 27.0 35.0 2.0 35.0 106-107 27.44473461985886 34.0 27.0 35.0 2.0 35.0 108-109 27.39987445291112 34.0 27.0 35.0 2.0 35.0 110-111 27.318979780442056 34.0 27.0 35.0 2.0 35.0 112-113 27.22512311835055 34.0 27.0 35.0 2.0 35.0 114-115 27.106695844740102 34.0 27.0 35.0 2.0 35.0 116-117 26.998700438169305 34.0 26.5 35.0 2.0 35.0 118-119 26.85626925864942 34.0 26.0 35.0 2.0 35.0 120-121 26.67927551358474 34.0 25.0 35.0 2.0 35.0 122-123 26.444683304977296 34.0 25.0 35.0 2.0 35.0 124-125 24.975385546640496 32.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.982480319013117 1101 2 0.9155114926484416 1101 3 0.9294346748954325 1101 4 1.1580444840861972 1101 5 1.0796591266712952 1101 6 1.1596788579736312 1101 7 1.2130876978917087 1101 8 1.1490540413424668 1101 9 1.4504368485980734 1101 10-11 1.4581818958280195 1101 12-13 1.3020049896661945 1101 14-15 1.6011314455109726 1101 16-17 1.3898841906631105 1101 18-19 1.752968213126266 1101 20-21 2.1542217735306366 1101 22-23 2.0592729727216863 1101 24-25 2.189708155300629 1101 26-27 2.213110572532109 1101 28-29 1.9936398784359568 1101 30-31 2.0728369957362176 1101 32-33 2.045667021221867 1101 34-35 2.425424432008427 1101 36-37 2.2993909068348515 1101 38-39 2.10940678832052 1101 40-41 1.8110367099159674 1101 42-43 1.9287639675644428 1101 44-45 1.8463195474636152 1101 46-47 1.8296676615102356 1101 48-49 1.8685033529416657 1101 50-51 1.754255669085488 1101 52-53 1.5716292332220831 1101 54-55 1.501727548717028 1101 56-57 1.4955727986937362 1101 58-59 1.518381302249736 1101 60-61 1.5151616072911054 1101 62-63 1.4548826512524826 1101 64-65 1.5010594838955704 1101 66-67 1.543462263325587 1101 68-69 1.2880762992898376 1101 70-71 1.2631714661255984 1101 72-73 1.1425390010877443 1101 74-75 1.2218992542810128 1101 76-77 1.4220088252087528 1101 78-79 1.4454558772023631 1101 80-81 1.4616184651452713 1101 82-83 1.3082602206771838 1101 84-85 1.348633872104923 1101 86-87 1.3940989761311258 1101 88-89 1.3349932600183472 1101 90-91 1.3186517565716258 1101 92-93 1.254286308553258 1101 94-95 1.259052184637202 1101 96-97 1.222213662881412 1101 98-99 1.2805371078699643 1101 100-101 1.4142418049937326 1101 102-103 1.3384670304457273 1101 104-105 1.3896780557129773 1101 106-107 1.3630603532805878 1101 108-109 1.410167160410058 1101 110-111 1.2930065131696793 1101 112-113 1.3833979866834127 1101 114-115 1.6138493641139178 1101 116-117 1.5501947740462256 1101 118-119 1.5660972926253756 1101 120-121 1.6328592551844139 1101 122-123 1.6794654066512713 1101 124-125 1.9314250465365 1102 1 0.12133353966074978 1102 2 0.3035534229358383 1102 3 0.43993392280508914 1102 4 0.5281379700833071 1102 5 0.5608168455195823 1102 6 0.003118995918001133 1102 7 -0.6472402868730391 1102 8 0.27945249914036197 1102 9 0.6760489434114803 1102 10-11 0.6778264622671131 1102 12-13 0.710218283154866 1102 14-15 0.9716168189380028 1102 16-17 0.77520875683539 1102 18-19 0.9658039439212871 1102 20-21 1.39636484746228 1102 22-23 1.2083152665472099 1102 24-25 1.3265577472374233 1102 26-27 0.7907793259298543 1102 28-29 1.3134942297205185 1102 30-31 1.1476624807594433 1102 32-33 1.0236529532969314 1102 34-35 1.4385993538810808 1102 36-37 1.4707374320440465 1102 38-39 1.5410049219357944 1102 40-41 1.2662856668283595 1102 42-43 1.0635048716070514 1102 44-45 1.1815533958648885 1102 46-47 0.8115349467475781 1102 48-49 0.6566139885997586 1102 50-51 1.0136484603579063 1102 52-53 0.8007733177230207 1102 54-55 0.6397844874591883 1102 56-57 0.5811451711302098 1102 58-59 0.715060431492546 1102 60-61 0.4725025760368311 1102 62-63 0.5886063155833909 1102 64-65 0.6059535742331228 1102 66-67 0.6754941412835045 1102 68-69 0.5221523287864542 1102 70-71 0.5206473442035815 1102 72-73 0.6635458349374801 1102 74-75 0.29057544186668594 1102 76-77 0.5140481740419283 1102 78-79 0.5501531900219199 1102 80-81 0.18994292125554324 1102 82-83 0.3694363498966453 1102 84-85 0.04933693483025792 1102 86-87 0.4994274778537644 1102 88-89 0.7213575785844704 1102 90-91 0.2420469358559565 1102 92-93 0.6088618861286257 1102 94-95 0.5011493994015233 1102 96-97 0.6703861333731211 1102 98-99 0.7842992271971845 1102 100-101 0.7850490263951215 1102 102-103 0.6124788904602028 1102 104-105 0.889292838707398 1102 106-107 0.878654971582236 1102 108-109 0.3717079950802571 1102 110-111 0.6792874514315699 1102 112-113 0.321833305246205 1102 114-115 0.3175632896252836 1102 116-117 0.12388545312877497 1102 118-119 0.5007062890266845 1102 120-121 0.8729678496926532 1102 122-123 0.6095927116997828 1102 124-125 1.0065098443059135 1103 1 0.7373145876638532 1103 2 0.776883072464976 1103 3 0.7736538316393329 1103 4 0.774487056998133 1103 5 0.89644975723089 1103 6 0.9125805502706328 1103 7 1.032616414263508 1103 8 1.1048326223854446 1103 9 1.2627571844397707 1103 10-11 1.2843877766126184 1103 12-13 1.1718146244318888 1103 14-15 1.5026989168252953 1103 16-17 1.451441837509016 1103 18-19 1.4572202042777889 1103 20-21 1.7958965060643237 1103 22-23 1.7856566796164302 1103 24-25 1.9309986347767065 1103 26-27 1.904024393523187 1103 28-29 1.8640494036459074 1103 30-31 1.7559251751878904 1103 32-33 1.6611057370604243 1103 34-35 2.128625705957589 1103 36-37 2.0823541798047955 1103 38-39 2.0941239228074764 1103 40-41 1.74570157112111 1103 42-43 1.7065895929451855 1103 44-45 1.5068890111413609 1103 46-47 1.4831559941460526 1103 48-49 1.4981366481944107 1103 50-51 1.559395094127865 1103 52-53 1.4639894971254037 1103 54-55 1.4059547363175184 1103 56-57 1.4558329855202246 1103 58-59 1.3234419537994313 1103 60-61 1.2587110681572469 1103 62-63 1.2808101990296237 1103 64-65 1.38712594771712 1103 66-67 1.4542874378795645 1103 68-69 1.4284025912155123 1103 70-71 1.340582379364875 1103 72-73 1.2832248233133399 1103 74-75 1.3292772200020195 1103 76-77 1.2899816237118138 1103 78-79 1.2650443806352705 1103 80-81 1.3618314363521478 1103 82-83 1.3010276021493326 1103 84-85 1.2762079070690433 1103 86-87 1.2685562469648985 1103 88-89 1.2781858567643134 1103 90-91 1.3250417007111217 1103 92-93 1.3409213958019706 1103 94-95 1.2442981114416831 1103 96-97 1.3194076298821855 1103 98-99 1.3889601352605183 1103 100-101 1.3162726260316688 1103 102-103 1.2520735152412286 1103 104-105 1.2750282867010938 1103 106-107 1.312886890010045 1103 108-109 1.4241161281137344 1103 110-111 1.3750775854425434 1103 112-113 1.4773525452852354 1103 114-115 1.487045855098188 1103 116-117 1.5476118114195998 1103 118-119 1.5266960034870714 1103 120-121 1.524931490881169 1103 122-123 1.4679129305782155 1103 124-125 1.6777315796464158 1104 1 0.7599872226338746 1104 2 0.8541461745425849 1104 3 0.7412249738055259 1104 4 0.7739068911999425 1104 5 0.7720054948465744 1104 6 0.9909120988991518 1104 7 0.9500847458902157 1104 8 0.9523885424133169 1104 9 1.0998513357189879 1104 10-11 1.113398986022375 1104 12-13 1.0873091815442777 1104 14-15 1.1933975588842785 1104 16-17 1.2916137417552918 1104 18-19 1.6022926593703772 1104 20-21 1.8086180019257085 1104 22-23 1.6397809093294953 1104 24-25 2.0792725255391886 1104 26-27 1.9948173988080455 1104 28-29 1.9331755870477778 1104 30-31 1.801570259694408 1104 32-33 1.725335703602731 1104 34-35 1.973322100001397 1104 36-37 1.9749721895679215 1104 38-39 2.032014441323163 1104 40-41 1.7162103189282334 1104 42-43 1.658737400225128 1104 44-45 1.6205350267195016 1104 46-47 1.5464869130451646 1104 48-49 1.600566747381606 1104 50-51 1.4954887055258652 1104 52-53 1.4596104165439172 1104 54-55 1.356637192124147 1104 56-57 1.2782819392471652 1104 58-59 1.2640495877945952 1104 60-61 1.3244878040179486 1104 62-63 1.3784948656221232 1104 64-65 1.4647542136392353 1104 66-67 1.3896041566917532 1104 68-69 1.3801211867005456 1104 70-71 1.39323126567173 1104 72-73 1.2890428893390862 1104 74-75 1.3135349466913127 1104 76-77 1.3308073828469347 1104 78-79 1.3751995000184998 1104 80-81 1.4041191521928909 1104 82-83 1.3561334515717007 1104 84-85 1.2733189031855403 1104 86-87 1.3029351725186586 1104 88-89 1.2631356068471753 1104 90-91 1.2963553479374994 1104 92-93 1.3173314946021577 1104 94-95 1.2854796988101604 1104 96-97 1.1564964626920258 1104 98-99 1.2312778721635311 1104 100-101 1.319434738975815 1104 102-103 1.3450834169864692 1104 104-105 1.2558579203977374 1104 106-107 1.2382577881829704 1104 108-109 1.3432140048110206 1104 110-111 1.3974088199418944 1104 112-113 1.4137576266187786 1104 114-115 1.483548395685748 1104 116-117 1.4984819413000174 1104 118-119 1.4886594420653658 1104 120-121 1.4790823808364912 1104 122-123 1.5228823669189069 1104 124-125 1.8891541468625341 1105 1 0.2385799873202359 1105 2 0.5882981825080691 1105 3 0.6403288569784458 1105 4 0.8170532569483697 1105 5 0.769018438756305 1105 6 0.6912108045081808 1105 7 0.8086974242838423 1105 8 0.5776789506443194 1105 9 0.6162801442155015 1105 10-11 0.8248161781896428 1105 12-13 0.8154870773292018 1105 14-15 1.0126806654226073 1105 16-17 0.9242058426514106 1105 18-19 1.0315989985671976 1105 20-21 1.4445290540332394 1105 22-23 1.5093461267207928 1105 24-25 1.669713946050308 1105 26-27 1.4924941329600543 1105 28-29 1.1985257364005832 1105 30-31 1.1172025199001823 1105 32-33 1.1571312562081033 1105 34-35 1.7843078285111886 1105 36-37 1.758908465704657 1105 38-39 1.8187718259896073 1105 40-41 1.4359248891240526 1105 42-43 1.083065312604809 1105 44-45 1.3721115285449272 1105 46-47 1.2688566442101177 1105 48-49 1.239796750700556 1105 50-51 1.2055783172085768 1105 52-53 1.1937624245094014 1105 54-55 1.0066537868802001 1105 56-57 0.9621518363596735 1105 58-59 0.9436048483322708 1105 60-61 0.977823349985421 1105 62-63 0.8987404680117734 1105 64-65 0.985331711150021 1105 66-67 0.7955118898480755 1105 68-69 0.6899452822863381 1105 70-71 0.7767360781509858 1105 72-73 0.744568943106092 1105 74-75 0.8057354113444788 1105 76-77 0.909964369239237 1105 78-79 0.9415785242468466 1105 80-81 0.9016299387843247 1105 82-83 0.7788018883456758 1105 84-85 0.8199501677059544 1105 86-87 0.7480065299697074 1105 88-89 0.7712670373151447 1105 90-91 0.6903148567327229 1105 92-93 0.6248323243399589 1105 94-95 0.7678892573896476 1105 96-97 0.8458426293033767 1105 98-99 0.6003452468731254 1105 100-101 0.6506660698752498 1105 102-103 0.6934073466247028 1105 104-105 0.7736143093788215 1105 106-107 0.689801100496279 1105 108-109 0.7320291392285441 1105 110-111 0.7752714153617397 1105 112-113 0.842068280452164 1105 114-115 0.7990147083309509 1105 116-117 0.7907155954653007 1105 118-119 0.8217161961310815 1105 120-121 0.7897362142251403 1105 122-123 0.9857101133510362 1105 124-125 1.1023967621960864 1106 1 0.31948984410963277 1106 2 0.5708508187534953 1106 3 0.33351378610092297 1106 4 0.5155948110962782 1106 5 0.32506331241872033 1106 6 0.37847569804825554 1106 7 0.15850879834992782 1106 8 0.34242986369388007 1106 9 0.5758899950790095 1106 10-11 0.7424348746326928 1106 12-13 0.7236455235643362 1106 14-15 1.0640574330795758 1106 16-17 0.8719323047417973 1106 18-19 0.916734558459396 1106 20-21 1.1965108486958371 1106 22-23 1.2368158443891346 1106 24-25 1.4696622465692784 1106 26-27 1.6841787181328414 1106 28-29 1.4626178162293542 1106 30-31 1.2268873795117017 1106 32-33 1.0912114887554978 1106 34-35 1.3955762875016404 1106 36-37 1.3663089270231055 1106 38-39 1.474710878509029 1106 40-41 1.3299804965405642 1106 42-43 1.2420685366966566 1106 44-45 1.095852439451697 1106 46-47 0.9765245819021615 1106 48-49 1.1044845969175228 1106 50-51 1.0239480925360809 1106 52-53 0.8894264276782877 1106 54-55 0.7903479012508612 1106 56-57 0.8919624961640196 1106 58-59 0.8051614905270128 1106 60-61 0.9397994463264894 1106 62-63 0.8282228378015191 1106 64-65 0.8767288904695967 1106 66-67 0.7984020023702101 1106 68-69 0.7911735758835086 1106 70-71 0.897257550052899 1106 72-73 0.9071763161348017 1106 74-75 0.9345041840918569 1106 76-77 0.702900005244242 1106 78-79 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-3.446190103778335 2216 108-109 -3.479742520965541 2216 110-111 -3.4861670146064156 2216 112-113 -3.6267401389847223 2216 114-115 -3.7646747908909717 2216 116-117 -3.802455082036605 2216 118-119 -3.779487453595781 2216 120-121 -3.8794231680149913 2216 122-123 -3.9292737171994823 2216 124-125 -4.088783911799677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104906.0 3 18652.0 4 11592.0 5 9124.0 6 8582.0 7 9080.0 8 9770.0 9 10137.0 10 10821.0 11 11558.0 12 12605.0 13 13834.0 14 16008.0 15 14501.0 16 10906.0 17 9811.0 18 9108.0 19 8810.0 20 8523.0 21 9382.0 22 10995.0 23 14692.0 24 19374.0 25 25271.0 26 30787.0 27 35778.0 28 41346.0 29 48440.0 30 55949.0 31 64411.0 32 79724.0 33 101456.0 34 133145.0 35 183654.0 36 288971.0 37 406198.0 38 148758.0 39 556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.70676690980061 18.837840005939253 13.258232365232061 23.197160719028076 2 15.04200755403587 19.928066765679905 41.31213632937231 23.717789350911918 3 19.503046977411362 25.42037625973371 28.549114605312916 26.527462157542008 4 12.25758877969184 16.391863781871628 37.93809399250153 33.412453445935 5 13.556939634769178 38.290938987020105 34.05311542390194 14.099005954308778 6 31.567598698878186 36.66581805119692 18.0019240819633 13.764659167961593 7 28.772631905163102 32.40365169672961 20.72028487339984 18.103431524707446 8 25.974845743978598 35.99818654204955 19.572542054538253 18.454425659433593 9 26.647907224461143 16.62512386861681 19.753712571001817 36.973256335920226 10-11 24.916737868140682 27.112367135558475 27.269500277748026 20.70139471855282 12-13 25.75195040522692 24.19561198058737 27.729483690033664 22.322953924152042 14-15 22.737258003152753 25.55796650270067 25.58920998585572 26.11556550829086 16-17 22.618349321557414 29.110337434671212 25.819391292950545 22.451921950820825 18-19 22.412752395944477 27.750307098911136 28.02047442328805 21.81646608185634 20-21 23.323913163707168 26.854857474261863 28.036223475255362 21.785005886775608 22-23 23.00321096460964 26.78656924996948 27.750157558969402 22.460062226451473 24-25 22.73067222326857 26.86219906307082 27.89021027592674 22.51691843773387 26-27 22.548101167423752 27.186278535308738 27.70339224601009 22.562228051257417 28-29 22.413020457993817 27.244725951762412 27.858812017972205 22.483441572271566 30-31 22.63268169819222 26.962409727271798 27.770258942701492 22.634649631834492 32-33 22.51067433071031 27.0048671310832 27.6926729460804 22.791785592126093 34-35 22.841857185920382 26.915294604175855 27.744884816506104 22.497963393397658 36-37 22.783377147264634 26.793597519048596 27.680181885945593 22.742843447741173 38-39 22.68212423681569 26.998652361605508 27.789150639069554 22.53007276250925 40-41 22.871144561366965 26.893505713002153 27.667340494529686 22.568009231101197 42-43 22.665953498939714 26.871193338541747 27.566083443613575 22.89676971890496 44-45 22.834419477698972 27.051709142024432 27.36971686499795 22.744154515278648 46-47 22.85841403712956 27.227803425182373 26.939158023330776 22.974624514357288 48-49 22.83371061158944 27.213194813867066 26.996943983834136 22.956150590709363 50-51 22.808559422037977 27.296404328288048 27.000732143021732 22.89430410665224 52-53 23.052089586815562 27.22625628046821 26.847049269759342 22.874604862956883 54-55 22.85508205053461 27.140694897581646 26.989836023504587 23.014387028379158 56-57 22.869777873326488 27.36148626851253 26.899356565786658 22.869379292374326 58-59 22.923779045595865 27.102563483002907 26.973703057053555 22.999954414347673 60-61 22.942582635487124 27.135264915839947 27.135190182190673 22.78696226648226 62-63 22.861687068571783 27.15379906006799 27.123723827134437 22.860790044225794 64-65 22.80089723348027 27.07222390674273 27.046985166447374 23.079893693329623 66-67 22.77659244534289 27.37046766035306 26.843881131292903 23.00905876301115 68-69 22.754249767161642 27.19963104650937 27.08765934290998 22.95845984341901 70-71 22.704652486544994 27.137250408227576 27.074923305528603 23.083173799698827 72-73 22.80289284519332 27.12242921665023 26.93789560933585 23.1367823288206 74-75 22.647828620209747 27.320896695395536 26.882054708154012 23.149219976240705 76-77 22.837617807255473 27.086551089835442 26.985658853704763 23.090172249204322 78-79 22.738645461597365 27.20293962762923 26.91968335880007 23.138731551973326 80-81 22.676366006077284 27.363765583686693 26.920723793523845 23.03914461671218 82-83 22.835664256403447 27.261648865161852 26.867837745534096 23.034849132900604 84-85 22.78983566782831 27.19422184469526 26.845679212241834 23.17026327523459 86-87 22.69777626647435 27.099605275777584 27.114527321169003 23.088091136579063 88-89 22.833839969684036 27.067311752892014 26.85431769524933 23.244530582174622 90-91 22.653479457379312 27.30793811363441 27.018165011205973 23.02041741778031 92-93 22.692921180905092 27.119285005915188 26.97450054717664 23.21329326600308 94-95 22.6045384937766 27.01598294912894 27.045733270676596 23.333745286417855 96-97 22.761093626359607 26.997405856406232 26.982058253123835 23.259442264110326 98-99 22.53757108801088 26.950894158265637 26.933905275770414 23.577629477953064 100-101 22.43798098230441 26.98029995134591 27.129077333596406 23.45264173275327 102-103 22.648371001611686 26.849615013837578 27.06254686219463 23.4394671223561 104-105 22.557058129405576 26.784470095065956 27.18901666039337 23.469455115135098 106-107 22.473424594201013 26.853004638770273 27.218461618174057 23.455109148854657 108-109 22.476117406456208 26.94091066477682 26.892883921254075 23.690088007512898 110-111 22.331127358726743 26.997900807617377 27.05160745027714 23.61936438337874 112-113 22.342997636028027 26.9529173506575 27.15937256347726 23.544712449837213 114-115 22.300874594898904 27.061027343857035 26.997606135864867 23.640491925379195 116-117 22.116689044272785 26.966941757609426 27.169311708013343 23.74705749010445 118-119 22.25414317848362 26.98629693381128 26.941533418193863 23.81802646951124 120-121 22.143248765889247 26.95688477738677 27.144009770754447 23.755856685969537 122-123 22.183373480170285 27.005975941789995 27.0314590116156 23.779191566424124 124-125 22.182085011542423 26.957770798476627 26.87258235111643 23.98756183886452 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 275.0 1 292.5 2 369.0 3 422.0 4 375.0 5 311.0 6 301.0 7 399.5 8 567.5 9 657.0 10 642.0 11 654.5 12 722.5 13 802.0 14 928.0 15 1064.5 16 1184.5 17 1334.0 18 1502.5 19 1689.5 20 2008.5 21 2470.0 22 3118.0 23 4042.0 24 5132.0 25 6674.0 26 9018.5 27 11812.5 28 14553.5 29 17827.0 30 21983.0 31 26397.0 32 30232.5 33 34006.5 34 38670.0 35 42906.5 36 47323.0 37 52226.5 38 55460.0 39 57535.0 40 59112.0 41 61159.5 42 63621.0 43 67034.0 44 70694.0 45 72726.5 46 74015.5 47 74811.5 48 76930.5 49 78688.0 50 78151.0 51 76865.5 52 74438.5 53 72590.0 54 70587.0 55 65866.5 56 59757.0 57 53705.5 58 49250.0 59 44336.5 60 37119.5 61 30083.0 62 24217.5 63 18745.5 64 14192.5 65 10741.0 66 7905.0 67 5973.0 68 4505.0 69 3247.0 70 2324.0 71 1629.5 72 1207.5 73 915.0 74 668.0 75 485.5 76 337.5 77 231.0 78 148.0 79 90.0 80 66.0 81 49.5 82 41.5 83 34.5 84 25.5 85 20.0 86 17.5 87 14.5 88 9.5 89 11.5 90 12.5 91 6.5 92 6.0 93 7.0 94 7.0 95 8.0 96 5.5 97 3.5 98 2.5 99 3.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011359022326955508 2 0.003786340775651836 3 0.007174119364392952 4 0.006974838270937593 5 0.013202372441417585 6 0.001195686560732159 7 0.007373400457848313 8 0.0 9 0.002391373121464318 10-11 0.0 12-13 0.0033877785887411168 14-15 0.01975373838876254 16-17 0.03158605331267453 18-19 0.006252444307161913 20-21 0.00866872756530815 22-23 0.0014946082009151985 24-25 0.011608123693774708 26-27 0.020077570165627497 28-29 2.2419123013727975E-4 30-31 0.001668979157688638 32-33 0.008992559342173112 34-35 0.009964054672767989 36-37 0.012106326427413107 38-39 0.0 40-41 0.025931452285878693 42-43 0.010312796586314869 44-45 0.0172129044472067 46-47 0.004035442142471035 48-49 0.025781991465787173 50-51 0.004658195559519036 52-53 0.0 54-55 0.018558051828030383 56-57 0.004708015832882875 58-59 2.2419123013727975E-4 60-61 0.004334363782654075 62-63 0.028920668687709088 64-65 0.01890679374157726 66-67 0.002117361617963198 68-69 0.022817685200638697 70-71 0.003113767085239997 72-73 0.013127642031371825 74-75 2.491013668191997E-5 76-77 0.006127893623752314 78-79 0.0010960460140044788 80-81 0.0033877785887411168 82-83 0.001718799431052478 84-85 0.0 86-87 0.005853882120251194 88-89 0.017511826087389742 90-91 0.023540079164414373 92-93 0.004658195559519036 94-95 0.025607620509013735 96-97 0.01928044579180606 98-99 9.96405467276799E-4 100-101 0.00944094180244767 102-103 0.0 104-105 0.0014696980642332784 106-107 9.964054672767988E-5 108-109 0.0 110-111 7.223939637756793E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.491013668191997E-5 122-123 0.0 124-125 0.024287383264871974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2007215.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.1788845690288 #Duplication Level Percentage of deduplicated Percentage of total 1 78.99909469575492 49.12075590145121 2 11.913521024742849 14.815388972163667 3 3.9536477731735555 7.3750022554426895 4 1.7569134695524982 4.369716792843067 5 0.9482360386714274 2.948012959637191 6 0.5692050900619078 2.1235522554637805 7 0.3757356436613149 1.6353976250980649 8 0.271132241084453 1.3486960257065836 9 0.18031518221539056 1.009061721091275 >10 0.9586557433946143 10.496108037118946 >50 0.0518866711657124 2.195239831622355 >100 0.021249584075820893 2.234965409693679 >500 1.6273697820810067E-4 0.060915198774346424 >1k 2.4410546731214647E-4 0.26718701389312144 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2460 0.12255787247504628 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.982027336383995E-5 0.0 0.0 0.0 0.0 6 4.982027336383995E-5 0.0 0.0 0.0 0.0 7 9.96405467276799E-5 0.0 0.0 0.0 0.0 8 9.96405467276799E-5 0.0 0.0 0.0 4.982027336383995E-5 9 9.96405467276799E-5 0.0 0.0 0.0 4.982027336383995E-5 10-11 9.96405467276799E-5 0.0 0.0 4.982027336383995E-5 4.982027336383995E-5 12-13 1.4946082009151983E-4 0.0 0.0 4.982027336383995E-5 4.982027336383995E-5 14-15 1.992810934553598E-4 0.0 0.0 7.473041004575992E-5 4.982027336383995E-5 16-17 1.992810934553598E-4 0.0 0.0 1.2455068340959987E-4 4.982027336383995E-5 18-19 2.740115035011197E-4 0.0 0.0 1.743709567734398E-4 4.982027336383995E-5 20-21 3.4874191354687965E-4 0.0 0.0 1.992810934553598E-4 7.473041004575992E-5 22-23 3.4874191354687965E-4 0.0 0.0 2.2419123013727978E-4 1.2455068340959987E-4 24-25 4.483824602745595E-4 0.0 0.0 3.985621869107196E-4 1.992810934553598E-4 26-27 4.483824602745595E-4 0.0 0.0 5.231128703203194E-4 1.992810934553598E-4 28-29 8.71854783867199E-4 0.0 0.0 8.967649205491191E-4 1.992810934553598E-4 30-31 9.46585193912959E-4 0.0 0.0 0.002466103531510077 2.2419123013727978E-4 32-33 9.46585193912959E-4 0.0 0.0 0.005953522666978874 2.4910136681919974E-4 34-35 9.96405467276799E-4 0.0 0.0 0.011109920960136308 2.4910136681919974E-4 36-37 0.0010960460140044788 0.0 0.0 0.02089960467613086 2.4910136681919974E-4 38-39 0.0011707764240502386 4.982027336383995E-5 0.0 0.03783849761983644 2.4910136681919974E-4 40-41 0.0011956865607321587 9.96405467276799E-5 0.0 0.05841427051910234 2.4910136681919974E-4 42-43 0.0012455068340959986 9.96405467276799E-5 0.0 0.07889040287164056 2.4910136681919974E-4 44-45 0.0014447879275513585 9.96405467276799E-5 0.0 0.10404964092037973 2.4910136681919974E-4 46-47 0.0017686197044163182 9.96405467276799E-5 0.0 0.1345396482190498 2.4910136681919974E-4 48-49 0.001942990661189758 9.96405467276799E-5 0.0 0.16771995027936717 2.9892164018303967E-4 50-51 0.001942990661189758 9.96405467276799E-5 0.0 0.20650503309311657 2.9892164018303967E-4 52-53 0.001942990661189758 1.4946082009151983E-4 0.0 0.24215143868494404 3.7365205022879956E-4 54-55 0.001942990661189758 2.9892164018303967E-4 0.0 0.28208238778606176 4.483824602745595E-4 56-57 0.002092451481281278 2.9892164018303967E-4 0.0 0.33165355978308253 4.732925969564795E-4 58-59 0.002142271754645118 2.9892164018303967E-4 0.0 0.38864795251131545 4.982027336383995E-4 60-61 0.002142271754645118 2.9892164018303967E-4 0.0 0.45333957747426157 4.982027336383995E-4 62-63 0.002142271754645118 2.9892164018303967E-4 0.0 0.5211449695224477 5.480230070022394E-4 64-65 0.002142271754645118 3.487419135468796E-4 0.0 0.5872066520028996 5.480230070022394E-4 66-67 0.0023415528481004776 4.2347232359263954E-4 0.0 0.6654244811841283 5.480230070022394E-4 68-69 0.0023415528481004776 4.483824602745595E-4 0.0 0.7579905490941429 5.480230070022394E-4 70-71 0.0024411933948281575 4.483824602745595E-4 0.0 0.8592253445694656 5.978432803660793E-4 72-73 0.0025657440782377574 4.483824602745595E-4 0.0 0.9688548561065955 7.473041004575992E-4 74-75 0.002640474488283517 4.483824602745595E-4 0.0 1.0778616142266773 7.473041004575992E-4 76-77 0.002640474488283517 4.483824602745595E-4 0.0 1.199996014378131 7.722142371395192E-4 78-79 0.002640474488283517 4.483824602745595E-4 0.0 1.3449480997302232 7.971243738214392E-4 80-81 0.002889575855102717 4.483824602745595E-4 0.0 1.510949250578538 7.971243738214392E-4 82-83 0.002889575855102717 4.483824602745595E-4 0.0 1.6872382878764856 7.971243738214392E-4 84-85 0.002939396128466557 4.483824602745595E-4 0.0 1.8630789427141587 7.971243738214392E-4 86-87 0.003014126538512317 4.483824602745595E-4 0.0 2.052296340950023 8.220345105033591E-4 88-89 0.0030390366751942366 4.483824602745595E-4 0.0 2.262288793178608 8.469446471852791E-4 90-91 0.0033379583153772766 4.483824602745595E-4 0.0 2.507828010452293 8.469446471852791E-4 92-93 0.003487419135468796 4.483824602745595E-4 0.0 2.768487680691904 8.469446471852791E-4 94-95 0.003487419135468796 4.483824602745595E-4 0.0 3.0367449426195003 8.96764920549119E-4 96-97 0.003487419135468796 4.732925969564795E-4 0.0 3.303258494979362 8.96764920549119E-4 98-99 0.0036617900922422363 4.982027336383995E-4 0.0 3.602927439262859 8.96764920549119E-4 100-101 0.003885981322379516 4.982027336383995E-4 0.0 3.9463634936964898 8.96764920549119E-4 102-103 0.004060352279152956 4.982027336383995E-4 0.0 4.318396385040964 9.21675057231039E-4 104-105 0.004633285422837115 5.480230070022394E-4 0.0 4.696831181512692 9.71495330594879E-4 106-107 0.004633285422837115 5.480230070022394E-4 0.0 5.071928019669044 0.001021315603958719 108-109 0.004683105696200954 5.480230070022394E-4 0.0 5.459405195756309 0.001046225740640639 110-111 0.004957117199702075 5.480230070022394E-4 0.0 5.906168497146544 0.0010711358773225588 112-113 0.005031847609747835 5.480230070022394E-4 0.0 6.399389203448559 0.0010960460140044788 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3040 0.0 55.38846 1 GTATCAA 5560 0.0 55.325096 1 TCAACGC 6750 0.0 44.77817 4 CAACGCA 6790 0.0 44.689632 5 ATCAACG 6805 0.0 44.41626 3 AACGCAG 6985 0.0 43.00971 6 TATCAAC 7125 0.0 42.58844 2 ACGCAGA 8230 0.0 36.069675 7 CGCAGAG 8355 0.0 34.7494 8 GTACATG 9415 0.0 34.125565 1 TACATGG 9950 0.0 31.812294 2 ACATGGG 10290 0.0 30.18294 3 GCAGAGT 9305 0.0 30.052256 9 CAGAGTA 8965 0.0 24.426016 10-11 AGAGTAC 8665 0.0 23.692213 10-11 CATGGGA 6010 0.0 23.660826 4 GAGTACT 3825 0.0 23.571379 12-13 CATGGGG 4910 0.0 23.387415 4 GAGTACA 7625 0.0 21.692589 1 GTGTAGC 1270 0.0 21.55066 1 >>END_MODULE