FastQCFastQC Report
Thu 26 May 2016
SRR1364559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007215
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46090.2296216399339383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42110.20979317113513002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23590.11752602486529842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40500.061.368121
GTATCAA70800.050.8470151
ATCAACG89950.040.1233673
TCAACGC90500.039.5504844
CAACGCA91850.038.801765
TATCAAC92850.038.4211922
AACGCAG93100.038.025166
GTACATG94250.031.7456421
ACGCAGA112650.031.1619477
CGCAGAG113900.030.6110128
TACATGG97250.030.1913972
ACATGGG99350.028.59413
GCAGAGT122300.027.48699
TGGTATC22200.025.7539712
GAGTACT65450.025.7258512-13
GTGGTAT22100.025.6208251
CATGGGG47850.024.519424
AGTACTT69650.024.51623212-13
CAGAGTA119050.024.01298310-11
AGAGTAC115250.023.69484710-11
Produced by FastQC (version 0.11.5)