##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364559_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2007215 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.14585034488084 31.0 30.0 34.0 26.0 34.0 2 30.003271697351803 31.0 30.0 34.0 26.0 34.0 3 30.275647103075656 31.0 30.0 34.0 26.0 34.0 4 33.81874338324494 35.0 35.0 37.0 29.0 37.0 5 33.258677321562466 35.0 33.0 37.0 27.0 37.0 6 33.287947230366456 36.0 35.0 37.0 27.0 37.0 7 33.07167543088309 36.0 33.0 37.0 26.0 37.0 8 33.00064467433733 36.0 33.0 37.0 26.0 37.0 9 34.23355146309688 38.0 34.0 39.0 25.0 39.0 10-11 34.17350782053741 38.0 34.0 39.0 25.0 39.0 12-13 34.059768385549134 38.0 34.0 39.0 25.0 39.0 14-15 35.03119695697771 39.0 34.0 41.0 25.0 41.0 16-17 35.02651185847057 39.0 34.0 41.0 25.0 41.0 18-19 35.03834890632045 39.0 34.0 41.0 25.0 41.0 20-21 35.0500863136236 39.0 34.0 41.0 25.0 41.0 22-23 35.01466409926191 39.0 34.0 41.0 25.0 41.0 24-25 34.975684717382045 39.0 34.0 41.0 24.5 41.0 26-27 34.860510956723616 39.0 34.0 41.0 24.0 41.0 28-29 34.71805810538483 39.0 34.0 41.0 24.0 41.0 30-31 34.57072909479054 38.5 33.5 41.0 23.5 41.0 32-33 34.3893867871653 38.0 33.0 41.0 23.0 41.0 34-35 34.22114073479921 38.0 33.0 40.0 22.0 41.0 36-37 34.0334266633121 38.0 33.0 40.0 20.0 41.0 38-39 33.87556290681367 38.0 33.0 40.0 18.5 41.0 40-41 33.9089255510745 38.0 33.0 40.0 18.5 41.0 42-43 33.787997548842554 38.0 33.0 40.0 17.0 41.0 44-45 33.634607403790824 38.0 33.0 40.0 15.5 41.0 46-47 33.40694893172879 38.0 32.5 40.0 14.0 41.0 48-49 33.13660220753631 38.0 32.0 40.0 9.0 41.0 50-51 32.97006399414114 38.0 32.0 40.0 8.0 41.0 52-53 32.78501406177216 38.0 32.0 40.0 7.0 41.0 54-55 32.59251550033255 38.0 31.0 40.0 4.5 41.0 56-57 32.37514067999691 37.0 31.0 40.0 2.0 41.0 58-59 32.171092782786104 37.0 31.0 40.0 2.0 41.0 60-61 31.93068903929076 36.5 31.0 40.0 2.0 41.0 62-63 31.75500681292238 36.0 31.0 40.0 2.0 41.0 64-65 31.621850175491915 36.0 31.0 39.5 2.0 41.0 66-67 31.317856084176334 35.5 30.0 39.0 2.0 41.0 68-69 31.03092543648787 35.0 30.0 39.0 2.0 41.0 70-71 30.714914446135566 35.0 30.0 38.5 2.0 40.0 72-73 30.423127816402328 35.0 30.0 37.5 2.0 40.0 74-75 30.070586110605987 35.0 29.5 37.0 2.0 39.0 76-77 29.76063600560976 35.0 29.0 36.5 2.0 39.0 78-79 29.476211566772868 35.0 29.0 36.0 2.0 39.0 80-81 29.19492854527293 34.0 29.0 36.0 2.0 37.5 82-83 28.904307460835035 34.0 29.0 35.0 2.0 37.0 84-85 28.67769920013551 34.0 29.0 35.0 2.0 36.5 86-87 28.467656678532194 34.0 29.0 35.0 2.0 36.0 88-89 28.294239530892305 34.0 29.0 35.0 2.0 36.0 90-91 28.148364774077514 34.0 28.5 35.0 2.0 36.0 92-93 28.034313464177977 34.0 28.0 35.0 2.0 35.0 94-95 27.927404637769246 34.0 28.0 35.0 2.0 35.0 96-97 27.80927603669761 34.0 27.5 35.0 2.0 35.0 98-99 27.727846543593984 34.0 27.0 35.0 2.0 35.0 100-101 27.63568651091189 34.0 27.0 35.0 2.0 35.0 102-103 26.359731269445476 31.5 24.5 34.0 2.0 35.0 104-105 27.302216503961958 33.5 26.5 35.0 2.0 35.0 106-107 27.46872781440952 34.0 27.0 35.0 2.0 35.0 108-109 27.477959760165206 34.0 27.0 35.0 2.0 35.0 110-111 27.401191700938863 34.0 27.0 35.0 2.0 35.0 112-113 27.314001489626172 34.0 27.0 35.0 2.0 35.0 114-115 27.21477071464691 34.0 27.0 35.0 2.0 35.0 116-117 27.092086049576153 34.0 27.0 35.0 2.0 35.0 118-119 26.960892829118954 34.0 26.5 35.0 2.0 35.0 120-121 26.760406334149557 34.0 25.0 35.0 2.0 35.0 122-123 26.470378858268795 34.0 25.0 35.0 2.0 35.0 124-125 24.638627401648556 31.5 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5666467059244233 1101 2 0.8256032235555715 1101 3 0.7090409249306191 1101 4 0.646183598943729 1101 5 0.7068455299399332 1101 6 0.5973555352737208 1101 7 0.6469994148850731 1101 8 0.7582448460927509 1101 9 0.963760983860162 1101 10-11 0.915214385642038 1101 12-13 0.8351648784048749 1101 14-15 0.9394862463134785 1101 16-17 0.9515130912505043 1101 18-19 0.951965818561824 1101 20-21 0.9889363460188179 1101 22-23 0.9865730191045046 1101 24-25 1.033116570108156 1101 26-27 0.9111878338098265 1101 28-29 1.0137523907810788 1101 30-31 1.127398092614591 1101 32-33 1.116657022442432 1101 34-35 1.0165998833328018 1101 36-37 1.1165689653312114 1101 38-39 1.0687149535553075 1101 40-41 0.8605580004297693 1101 42-43 0.8764739315437637 1101 44-45 0.9966501132182799 1101 46-47 1.1101093816053194 1101 48-49 1.264003644908371 1101 50-51 1.237216434830728 1101 52-53 1.152071681674947 1101 54-55 1.0995228783630466 1101 56-57 1.1368704312186395 1101 58-59 1.1316116048622433 1101 60-61 1.1476954705567124 1101 62-63 1.1574896889366961 1101 64-65 1.0718533557079084 1101 66-67 1.2103117774328993 1101 68-69 1.241023314405588 1101 70-71 1.1909757256628524 1101 72-73 1.1686313201027652 1101 74-75 1.3204147928297303 1101 76-77 1.3181150576688516 1101 78-79 1.1571318611326902 1101 80-81 0.9797585195328189 1101 82-83 1.2244629588893439 1101 84-85 1.2514766682421126 1101 86-87 1.2116104623847797 1101 88-89 1.2311634765201127 1101 90-91 1.2033684318418914 1101 92-93 1.116505154840464 1101 94-95 1.1505446064577107 1101 96-97 1.1721789799612523 1101 98-99 1.1696621073299553 1101 100-101 1.2491410732898949 1101 102-103 1.066848067891641 1101 104-105 1.1150137376965432 1101 106-107 1.1110641943861346 1101 108-109 1.1003619566228338 1101 110-111 1.0096331221047983 1101 112-113 1.0771500501503581 1101 114-115 1.1247363622566091 1101 116-117 1.1619692861809945 1101 118-119 1.1586295471924757 1101 120-121 1.1303677963709475 1101 122-123 1.0452375091934165 1101 124-125 1.4056148683978407 1102 1 0.17466755767090447 1102 2 -0.6413648351174821 1102 3 -0.047772079728670036 1102 4 0.06382053441738123 1102 5 0.2980452391094346 1102 6 0.11602271778653517 1102 7 0.05114708473908536 1102 8 0.19726382678521048 1102 9 0.5209298556579398 1102 10-11 0.45924418909799414 1102 12-13 0.5007198076816977 1102 14-15 0.5804206393548839 1102 16-17 0.5189460784999937 1102 18-19 0.6744077942081077 1102 20-21 0.4935831303740059 1102 22-23 0.6302347818975491 1102 24-25 0.5503954705554079 1102 26-27 0.6300036000247928 1102 28-29 0.27990398951502726 1102 30-31 0.6027642155304989 1102 32-33 0.7796930327282325 1102 34-35 0.873723563005683 1102 36-37 0.44276772396523967 1102 38-39 -0.08197993790021485 1102 40-41 -0.47649353860389 1102 42-43 -0.18058404444936826 1102 44-45 -0.25184237088110706 1102 46-47 -0.13480441983615066 1102 48-49 -0.457294352963757 1102 50-51 -0.46249273466808916 1102 52-53 -0.7727092782658218 1102 54-55 -0.6917745525230217 1102 56-57 -0.8000365263674674 1102 58-59 -0.7090361266931993 1102 60-61 -0.835149865649349 1102 62-63 -0.6252762692197358 1102 64-65 -0.6130107844243646 1102 66-67 -0.6006342221468586 1102 68-69 -0.46637018393180796 1102 70-71 -1.0138913494561308 1102 72-73 -0.936502905510995 1102 74-75 -0.8051642833831991 1102 76-77 -1.126915642959169 1102 78-79 -0.8618755130214169 1102 80-81 -0.9013361638054604 1102 82-83 -1.0720924339819753 1102 84-85 -0.9878923429675375 1102 86-87 -0.5885419568113655 1102 88-89 -0.7197021777984851 1102 90-91 -0.6960298958827273 1102 92-93 -0.6321490870491324 1102 94-95 -0.4593914908771204 1102 96-97 -0.6881031456361626 1102 98-99 -0.8199228588676704 1102 100-101 -0.6960388780827103 1102 102-103 -0.7819008940235719 1102 104-105 -0.5425917133742288 1102 106-107 -0.4096849943235057 1102 108-109 -0.4411405898116598 1102 110-111 -0.37705637964785055 1102 112-113 -0.11157709947726602 1102 114-115 -0.20496770436758638 1102 116-117 -0.3801869618387208 1102 118-119 -0.028177354028546375 1102 120-121 0.20595954639012248 1102 122-123 0.2604612519018126 1102 124-125 0.3654138885125029 1103 1 0.22145802927062164 1103 2 -0.2270384758189259 1103 3 0.13052516296068362 1103 4 0.0721930711532508 1103 5 0.1439527733847683 1103 6 0.36459804290464604 1103 7 0.4301089237747604 1103 8 0.5396988997362868 1103 9 0.6989600505356606 1103 10-11 0.6613872057977161 1103 12-13 0.5677450294237545 1103 14-15 0.7098708063857373 1103 16-17 0.7211587963580968 1103 18-19 0.7121631481808208 1103 20-21 0.7384487582974586 1103 22-23 0.7009516457149232 1103 24-25 0.6362920709863786 1103 26-27 0.7232855840388694 1103 28-29 0.7509712906021946 1103 30-31 0.6712519019391081 1103 32-33 0.7586521005130251 1103 34-35 0.6960947796184129 1103 36-37 0.7694877050199622 1103 38-39 0.871245966073424 1103 40-41 0.7707032991469305 1103 42-43 0.8579140887352636 1103 44-45 0.8687276166398732 1103 46-47 0.8534047474617452 1103 48-49 0.8141488305148812 1103 50-51 0.749730917034384 1103 52-53 0.6159166683682855 1103 54-55 0.6555320435579262 1103 56-57 0.7430635628164239 1103 58-59 0.8295560610666044 1103 60-61 0.8370762381902566 1103 62-63 0.7742033234567707 1103 64-65 0.6163438164399295 1103 66-67 0.6056274611948993 1103 68-69 0.7373956940760493 1103 70-71 0.6146098740931194 1103 72-73 0.5138543339200403 1103 74-75 0.7296867156947258 1103 76-77 0.6225317864117272 1103 78-79 0.4879352861233599 1103 80-81 0.49976335097370495 1103 82-83 0.5274924662278018 1103 84-85 0.6017928933193915 1103 86-87 0.6627462549592273 1103 88-89 0.6724974228054776 1103 90-91 0.6157177724875602 1103 92-93 0.49999633140134847 1103 94-95 0.4140799123331611 1103 96-97 0.4974375590005593 1103 98-99 0.5643795165142187 1103 100-101 0.5160583877843798 1103 102-103 0.328399089684833 1103 104-105 0.4487645032133045 1103 106-107 0.5090694879648439 1103 108-109 0.567239924767641 1103 110-111 0.5493127442505745 1103 112-113 0.450560643448064 1103 114-115 0.5668592553686587 1103 116-117 0.5042825283686874 1103 118-119 0.5351272534699554 1103 120-121 0.36798702712153286 1103 122-123 0.3249318842004847 1103 124-125 0.597232803223477 1104 1 0.330761967235091 1104 2 0.5270791793607152 1104 3 0.2853438829318442 1104 4 0.08645573496634995 1104 5 0.2906159034578977 1104 6 0.45023094472151826 1104 7 0.4924824616058814 1104 8 0.3607090943368192 1104 9 0.4878234924974123 1104 10-11 0.5806646091263303 1104 12-13 0.5043871787009735 1104 14-15 0.6404766060747562 1104 16-17 0.61548697692443 1104 18-19 0.5046523151942282 1104 20-21 0.46998079771886125 1104 22-23 0.5351707116581679 1104 24-25 0.6736629184641103 1104 26-27 0.6338091828230503 1104 28-29 0.7686159351468262 1104 30-31 0.6633096166109169 1104 32-33 0.6073193543236286 1104 34-35 0.8846233164316715 1104 36-37 0.902273307674399 1104 38-39 0.9719978052736877 1104 40-41 0.8323324775825967 1104 42-43 0.8070007774098329 1104 44-45 0.7158052704797129 1104 46-47 0.7324280139547383 1104 48-49 0.702801016083292 1104 50-51 0.500565831857962 1104 52-53 0.6565920392947504 1104 54-55 0.36327332469120677 1104 56-57 0.5057338825548783 1104 58-59 0.6268750772312472 1104 60-61 0.7026909078414576 1104 62-63 0.7573593914512493 1104 64-65 0.7160548198664927 1104 66-67 0.7540872720678635 1104 68-69 0.4966576104744611 1104 70-71 0.14227219519310452 1104 72-73 0.1664612495823583 1104 74-75 0.4961883702479213 1104 76-77 0.6370599210581709 1104 78-79 0.6609401160856692 1104 80-81 0.6516363178614775 1104 82-83 0.6309894843204447 1104 84-85 0.6789469667746957 1104 86-87 0.6790342371760758 1104 88-89 0.7668340563433595 1104 90-91 0.7502791781205431 1104 92-93 0.7218739909896605 1104 94-95 0.6711617089211721 1104 96-97 0.5804570554908786 1104 98-99 0.6247351478510481 1104 100-101 0.6101048252642443 1104 102-103 0.5223564258287325 1104 104-105 0.6331404470294473 1104 106-107 0.5733605603255612 1104 108-109 0.5663058544377861 1104 110-111 0.6109095026548026 1104 112-113 0.6105437103468283 1104 114-115 0.6477471206470717 1104 116-117 0.5817252702365465 1104 118-119 0.6006854228708995 1104 120-121 0.6831344579217387 1104 122-123 0.7205528488825657 1104 124-125 1.1317475194848328 1105 1 0.15319807742427116 1105 2 0.05379673661262174 1105 3 0.19705978070814467 1105 4 0.16909762013063556 1105 5 -0.10301171021617961 1105 6 0.3071841475094388 1105 7 0.535296932233166 1105 8 0.539932459753345 1105 9 0.7178268114486244 1105 10-11 0.5448199360430266 1105 12-13 0.5832122699721296 1105 14-15 0.6432977146044578 1105 16-17 0.6086831269410311 1105 18-19 0.5846390393893799 1105 20-21 0.6842190984158378 1105 22-23 0.665937389388013 1105 24-25 0.5598228919124963 1105 26-27 0.6537228900915508 1105 28-29 0.6224161342838954 1105 30-31 0.7046305591930633 1105 32-33 0.8608872268758958 1105 34-35 0.9377265358143418 1105 36-37 0.9494024148765234 1105 38-39 0.8895218676699699 1105 40-41 0.772591355777088 1105 42-43 0.7014581288867632 1105 44-45 0.6796287022752665 1105 46-47 0.6270513129922435 1105 48-49 0.5882971992893999 1105 50-51 0.4536026722164124 1105 52-53 0.6781652221689569 1105 54-55 0.6060546058063707 1105 56-57 0.49693866184462365 1105 58-59 0.1665365442076805 1105 60-61 0.2992723880936978 1105 62-63 0.38148816675825614 1105 64-65 0.24609132832981828 1105 66-67 0.3966362265982326 1105 68-69 0.4404013874409394 1105 70-71 0.46570399074571256 1105 72-73 0.47877455857671336 1105 74-75 0.44922521060636456 1105 76-77 0.5557456163784487 1105 78-79 0.39459428136944297 1105 80-81 0.4632858366135544 1105 82-83 0.47085008836956277 1105 84-85 0.3616552309632084 1105 86-87 0.4689446254933678 1105 88-89 0.482068042403764 1105 90-91 0.325951265740855 1105 92-93 0.4075693112684533 1105 94-95 0.347398018247425 1105 96-97 0.42712150952340266 1105 98-99 0.3943999337786934 1105 100-101 0.42606898059116105 1105 102-103 0.08624623664851327 1105 104-105 0.19885568101550888 1105 106-107 0.22470473556618487 1105 108-109 0.33646848304714894 1105 110-111 0.23205122187153293 1105 112-113 0.07038440068868113 1105 114-115 0.08634652323585712 1105 116-117 -0.14844399627523686 1105 118-119 -0.011495128075001304 1105 120-121 0.08306807889751155 1105 122-123 0.1541738246542259 1105 124-125 0.574661558648458 1106 1 -0.22903278053025034 1106 2 -0.7362754308608004 1106 3 -0.12392538675693743 1106 4 -0.3497449198561924 1106 5 -0.13632706308062836 1106 6 0.062175924486730594 1106 7 0.18537075805559766 1106 8 0.2472054692895327 1106 9 0.2336299086407152 1106 10-11 0.11282210316269214 1106 12-13 0.08777149049401345 1106 14-15 0.08354442556436226 1106 16-17 0.14358089625882542 1106 18-19 0.16783188622710554 1106 20-21 0.06826197304668824 1106 22-23 -0.014450481771795864 1106 24-25 0.0073578327177799 1106 26-27 0.10257020233816405 1106 28-29 0.264053109518386 1106 30-31 0.25066427730392604 1106 32-33 0.23412497756881834 1106 34-35 0.2389218088887759 1106 36-37 0.2695622945921414 1106 38-39 0.18841054851417027 1106 40-41 0.1654290004798682 1106 42-43 -0.11156454114977521 1106 44-45 0.038044846032491364 1106 46-47 0.10148885440872135 1106 48-49 0.23112785909825107 1106 50-51 0.3061822611396252 1106 52-53 0.20977152921273046 1106 54-55 0.2293631630449795 1106 56-57 -0.3938295390427271 1106 58-59 0.09219688143384985 1106 60-61 0.1765906687552956 1106 62-63 -0.033365243976714254 1106 64-65 0.16669420804386448 1106 66-67 0.029747124857845364 1106 68-69 0.19489751859832083 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-1.8642804553716168 2216 52-53 -1.8539148683355435 2216 54-55 -1.9886815852874093 2216 56-57 -1.852412105602049 2216 58-59 -1.9478824865502915 2216 60-61 -1.6762623443132263 2216 62-63 -1.8237284042136643 2216 64-65 -1.77084995853318 2216 66-67 -1.5339853513928539 2216 68-69 -1.4530839526955859 2216 70-71 -1.6170218087365988 2216 72-73 -1.4353080533030003 2216 74-75 -1.4043845869889964 2216 76-77 -1.4673738105340135 2216 78-79 -1.4572669877545579 2216 80-81 -1.4312368478261241 2216 82-83 -1.3963595637314903 2216 84-85 -1.413025282131784 2216 86-87 -1.5437931000074379 2216 88-89 -1.5109501395211886 2216 90-91 -1.522053431625757 2216 92-93 -1.4820886367204515 2216 94-95 -1.3930882590317246 2216 96-97 -1.3765525703852326 2216 98-99 -1.4445628467114524 2216 100-101 -1.4884842213158649 2216 102-103 -1.3891944878646818 2216 104-105 -1.3634178730545408 2216 106-107 -1.4568589171373638 2216 108-109 -1.4667627641673704 2216 110-111 -1.4980114656567842 2216 112-113 -1.3669683076608443 2216 114-115 -1.4125464867886244 2216 116-117 -1.5179765013052773 2216 118-119 -1.596956286573409 2216 120-121 -1.7225340574903711 2216 122-123 -1.7244388610801842 2216 124-125 -1.870063635035791 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61714.0 3 20818.0 4 10122.0 5 7994.0 6 8226.0 7 9880.0 8 11103.0 9 12049.0 10 12891.0 11 14341.0 12 15723.0 13 17863.0 14 20592.0 15 18100.0 16 12738.0 17 11396.0 18 10515.0 19 10240.0 20 9758.0 21 10146.0 22 11404.0 23 14192.0 24 18740.0 25 24779.0 26 30826.0 27 34635.0 28 40118.0 29 47656.0 30 55299.0 31 64746.0 32 79207.0 33 98365.0 34 126900.0 35 173265.0 36 283419.0 37 435151.0 38 161769.0 39 535.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.760569336382616 19.41572307535798 14.12379904671762 23.699908541541788 2 15.455053621586936 20.490291518492796 39.67314895173111 24.381505908189162 3 19.39049877566678 25.25857967382667 29.14705200987438 26.20386954063217 4 12.890819223620435 17.187243632613345 36.650343934381915 33.2715932093843 5 13.380031536233039 38.505591080178256 33.96925590930717 14.145121474281531 6 31.725300976726462 37.01466957949198 17.311897330380653 13.948132113400908 7 28.280935581729384 32.66554701897539 20.657649367595017 18.395868031700214 8 26.174479256193443 36.038954083068894 19.122895388875687 18.663671271861972 9 26.491063657211928 16.136534503555055 19.66862318093248 37.703778658300536 10-11 25.143128166562 27.06351046941156 26.869434579286768 20.92392678473967 12-13 25.89640372356723 24.139367232708008 27.72807098392549 22.236158059799273 14-15 22.926615162416745 25.332837122916203 25.727937194508005 26.012610520159047 16-17 22.426973188628185 28.90554939996253 26.116600770821957 22.550876640587322 18-19 22.29077896698224 27.179609177564018 28.730119782630087 21.799492072823654 20-21 23.094356561749652 26.279108175884737 28.85245762794196 21.774077634423655 22-23 22.83330385633826 26.178934493813564 28.641874437965043 22.34588721188313 24-25 22.509895551796895 26.411121877825742 28.729632849495445 22.34934972088192 26-27 22.338513811425283 26.64913823382149 28.65951081473584 22.352837140017385 28-29 22.272601589764925 26.607687766382774 28.809146005784136 22.31056463806817 30-31 22.412920389694175 26.35489969933465 28.81614575419175 22.416034156779418 32-33 22.290885630089452 26.46510214401546 28.692890397889613 22.55112182800547 34-35 22.627197385432055 26.517787083097726 28.516925192368532 22.33809033910169 36-37 22.545766148618856 26.358885321203758 28.536803481440703 22.558545048736683 38-39 22.481149254065958 26.528199520230768 28.64304521438909 22.347606011314184 40-41 22.63125773771121 26.351312639652452 28.593648413348845 22.423781209287498 42-43 22.522250979591124 26.385937729640325 28.395039893583895 22.696771397184655 44-45 22.662744150477153 26.512506134121157 28.1761047022865 22.648645013115186 46-47 22.69901330948603 26.775930829532463 27.68711871922041 22.837937141761095 48-49 22.82294123947858 26.831181512692957 27.510456029872238 22.835421217956224 50-51 22.784840081824857 26.981054337006416 27.439401080303345 22.79470450086538 52-53 22.994223339303463 26.921007465567964 27.275329249731595 22.80943994539698 54-55 22.81347538753945 26.858732123863167 27.378507035868104 22.949285452729278 56-57 22.810929204609064 27.15963696868704 27.24311792787853 22.786315898825364 58-59 22.841733339112523 26.812262449052188 27.40345884642345 22.942545365411835 60-61 22.874355761590063 26.869792224549936 27.52019589331487 22.735656120545134 62-63 22.70872826279198 26.8771407148711 27.59397971816671 22.820151304170206 64-65 22.718281275571883 26.73569948727325 27.507616917915144 23.03840231923972 66-67 22.64585507780681 27.126466272920442 27.290798444611063 22.936880204661684 68-69 22.6301922390457 26.937529596363518 27.45324428455454 22.97903388003624 70-71 22.602461619706908 26.834619111555064 27.485147331003407 23.077771937734624 72-73 22.726165358469324 26.957077343483384 27.19419693455858 23.122560363488713 74-75 22.624392683908663 27.055334018868148 27.149395772801004 23.170877524422178 76-77 22.76442732841275 26.88050358332316 27.231885971358327 23.123183116905764 78-79 22.678586997406853 27.026651355235987 27.149582879761265 23.145178767595898 80-81 22.529414563437882 27.14400052012456 27.139118124821426 23.187466791616128 82-83 22.75052747214424 27.025679859905395 27.110997078040967 23.112795589909403 84-85 22.723948356304632 26.950849809312906 27.067354518574245 23.25784731580822 86-87 22.61651093679551 26.82477960756571 27.32564772582907 23.233061729809712 88-89 22.678526367341 26.85491330041334 27.122700671760192 23.34385966048547 90-91 22.588766671424505 27.027704313614713 27.24928003459521 23.134248980365577 92-93 22.608370730467858 26.85887735109962 27.20229576680629 23.33045615162623 94-95 22.519884516606343 26.758717925085257 27.21392576281066 23.507471795497743 96-97 22.621922630990753 26.720040696929942 27.202345155605467 23.455691516473838 98-99 22.418104018440218 26.69203284583071 27.100201805110718 23.789661330618355 100-101 22.291164040868537 26.751853046644094 27.29440866295414 23.662574249533236 102-103 22.553662661947026 26.552910375819234 27.203837157454487 23.68958980477926 104-105 22.4912876796955 26.555625580717564 27.282702650189446 23.670384089397498 106-107 22.305340370060925 26.623307391503413 27.32304428644523 23.748307951990427 108-109 22.298862428504776 26.689773315208715 27.04272512977562 23.968639126510894 110-111 22.23417910867888 26.700150930781337 27.163804113318402 23.90186584722138 112-113 22.263683760832794 26.690638521533565 27.226580112245074 23.819097605388563 114-115 22.133403745986353 26.73707101630867 27.055945675973923 24.073579561731055 116-117 21.9383822859036 26.667372453872655 27.212431154609746 24.181814105613995 118-119 22.054015140381075 26.663087910363366 27.02386141992761 24.25903552932795 120-121 21.910341590955742 26.68140032164273 27.205788572715374 24.202469514686157 122-123 21.974609958370813 26.638269505319922 27.098430068274254 24.28869046803501 124-125 21.86692675571326 26.55538585277145 27.03288062082298 24.54480677069231 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 103.0 1 89.5 2 147.0 3 238.5 4 184.0 5 153.5 6 308.5 7 698.5 8 1177.5 9 1399.0 10 1226.5 11 936.5 12 860.5 13 952.5 14 1120.5 15 1300.0 16 1498.5 17 1723.0 18 1995.5 19 2308.0 20 2705.5 21 3169.0 22 3713.0 23 4588.5 24 5657.0 25 7105.0 26 9469.5 27 12364.5 28 14937.5 29 17935.5 30 22026.0 31 26245.5 32 29843.0 33 33655.0 34 38179.0 35 42270.5 36 46799.0 37 51653.0 38 54966.0 39 57122.5 40 58485.5 41 60345.5 42 62925.0 43 66382.5 44 70178.0 45 72486.0 46 73297.0 47 74201.0 48 76966.5 49 78954.0 50 78561.0 51 77111.5 52 74395.5 53 72515.0 54 70934.5 55 66387.0 56 60313.5 57 53977.5 58 49159.5 59 44365.5 60 37553.0 61 30109.0 62 23698.5 63 18665.5 64 14157.0 65 10464.0 66 7543.0 67 5793.5 68 4405.0 69 2939.0 70 2043.5 71 1495.5 72 1048.5 73 788.5 74 622.5 75 473.0 76 298.5 77 152.0 78 94.0 79 52.5 80 30.5 81 19.5 82 15.0 83 8.5 84 8.0 85 9.5 86 5.0 87 0.0 88 0.5 89 2.0 90 1.5 91 1.0 92 1.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09630258841230262 2 4.982027336383994E-5 3 0.0 4 0.10143407656877812 5 0.0 6 0.0 7 0.001793529841098238 8 0.006675916630754552 9 0.0036867002289241563 10-11 5.231128703203194E-4 12-13 0.0 14-15 9.964054672767988E-5 16-17 3.487419135468796E-4 18-19 0.001868260251143998 20-21 0.0012704169707779187 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.982027336383994E-5 52-53 0.0 54-55 0.0 56-57 0.008494356608534712 58-59 6.974838270937592E-4 60-61 0.0 62-63 0.0 64-65 2.740115035011197E-4 66-67 0.0 68-69 2.491013668191997E-5 70-71 0.0 72-73 0.0 74-75 0.0011458662873683188 76-77 0.0 78-79 0.0 80-81 1.743709567734398E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012704169707779187 90-91 2.491013668191997E-5 92-93 0.002117361617963198 94-95 0.0 96-97 0.00916693029894655 98-99 0.0040105320057891155 100-101 0.0023664629847823973 102-103 0.0 104-105 0.0 106-107 0.001594248747642878 108-109 2.491013668191997E-5 110-111 1.743709567734398E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.46585193912959E-4 122-123 0.004458914466063675 124-125 0.08708583783999223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2007215.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.43861093549838 #Duplication Level Percentage of deduplicated Percentage of total 1 78.55821372914437 47.47949315363484 2 12.069475747074993 14.589246977457984 3 4.01295721748167 7.276126799045246 4 1.805694457489992 4.365346591384938 5 0.9748627614702446 2.9459675578002846 6 0.5866748527256892 2.127468790171725 7 0.377697693033765 1.5979266744352114 8 0.27425633334018595 1.3260537461875082 9 0.21665258329987272 1.1784763071208495 >10 1.034715409380315 11.110201155085136 >50 0.06209706183402261 2.537502562761701 >100 0.02604121132905825 2.71399821383984 >500 3.3047119791205576E-4 0.1300755962659152 >1k 3.3047119791204346E-4 0.6221158748088257 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4609 0.2296216399339383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4211 0.20979317113513002 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2359 0.11752602486529842 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.982027336383995E-5 0.0 0.0 0.0 0.0 6 9.96405467276799E-5 0.0 0.0 0.0 0.0 7 2.9892164018303967E-4 0.0 0.0 0.0 0.0 8 2.9892164018303967E-4 0.0 0.0 0.0 0.0 9 2.9892164018303967E-4 0.0 0.0 0.0 0.0 10-11 3.985621869107196E-4 0.0 0.0 4.982027336383995E-5 0.0 12-13 4.982027336383995E-4 7.473041004575992E-5 0.0 4.982027336383995E-5 0.0 14-15 5.729331436841594E-4 9.96405467276799E-5 0.0 7.473041004575992E-5 2.4910136681919974E-5 16-17 6.476635537299193E-4 9.96405467276799E-5 0.0 1.2455068340959987E-4 4.982027336383995E-5 18-19 6.974838270937593E-4 9.96405467276799E-5 0.0 1.4946082009151983E-4 4.982027336383995E-5 20-21 6.974838270937593E-4 9.96405467276799E-5 0.0 1.743709567734398E-4 4.982027336383995E-5 22-23 6.974838270937593E-4 9.96405467276799E-5 0.0 1.992810934553598E-4 1.2455068340959987E-4 24-25 7.971243738214392E-4 9.96405467276799E-5 0.0 3.4874191354687965E-4 1.4946082009151983E-4 26-27 7.971243738214392E-4 9.96405467276799E-5 0.0 4.732925969564795E-4 1.4946082009151983E-4 28-29 8.220345105033591E-4 9.96405467276799E-5 0.0 8.220345105033591E-4 1.4946082009151983E-4 30-31 8.71854783867199E-4 1.2455068340959987E-4 0.0 0.0023913731214643174 1.4946082009151983E-4 32-33 8.96764920549119E-4 1.4946082009151983E-4 0.0 0.005903702393615033 1.4946082009151983E-4 34-35 9.46585193912959E-4 1.4946082009151983E-4 0.0 0.011060100686772469 1.4946082009151983E-4 36-37 0.0010960460140044788 1.4946082009151983E-4 0.0 0.020849784402767017 1.4946082009151983E-4 38-39 0.0014447879275513585 1.4946082009151983E-4 0.0 0.03818723953338332 1.4946082009151983E-4 40-41 0.0014447879275513585 1.4946082009151983E-4 0.0 0.0588626529793769 1.4946082009151983E-4 42-43 0.0015195183375971183 1.4946082009151983E-4 0.0 0.07916441437514168 1.743709567734398E-4 44-45 0.0015942487476428783 1.4946082009151983E-4 0.0 0.1038752699636063 1.992810934553598E-4 46-47 0.001843350114462078 1.4946082009151983E-4 0.0 0.13354324275177298 1.992810934553598E-4 48-49 0.001893170387825918 1.4946082009151983E-4 0.0 0.16577695961817743 1.992810934553598E-4 50-51 0.001942990661189758 1.4946082009151983E-4 0.0 0.2032916254611489 1.992810934553598E-4 52-53 0.001942990661189758 1.4946082009151983E-4 0.0 0.23851455872938376 2.4910136681919974E-4 54-55 0.001942990661189758 1.4946082009151983E-4 0.0 0.2774989226365885 2.9892164018303967E-4 56-57 0.001942990661189758 1.4946082009151983E-4 0.0 0.32532638506587486 2.9892164018303967E-4 58-59 0.001942990661189758 1.4946082009151983E-4 0.0 0.380676708773101 2.9892164018303967E-4 60-61 0.001967900797871678 1.4946082009151983E-4 0.0 0.4439982762185416 2.9892164018303967E-4 62-63 0.001992810934553598 1.4946082009151983E-4 0.0 0.5103837904758584 3.4874191354687965E-4 64-65 0.001992810934553598 1.4946082009151983E-4 0.0 0.575199966122214 3.4874191354687965E-4 66-67 0.0020675413445993577 1.4946082009151983E-4 0.0 0.6528448621597587 3.4874191354687965E-4 68-69 0.002092451481281278 1.4946082009151983E-4 0.0 0.7426209947613982 3.4874191354687965E-4 70-71 0.002167181891327038 1.4946082009151983E-4 0.0 0.8418629793021675 3.4874191354687965E-4 72-73 0.0022170021646908777 1.4946082009151983E-4 0.0 0.9490263873077871 4.483824602745595E-4 74-75 0.002241912301372798 1.4946082009151983E-4 0.0 1.0559905142199515 4.483824602745595E-4 76-77 0.0023415528481004776 1.4946082009151983E-4 0.0 1.1757833615233046 4.732925969564795E-4 78-79 0.0023415528481004776 1.4946082009151983E-4 0.0 1.3176465899268388 4.982027336383995E-4 80-81 0.002715204898329277 1.4946082009151983E-4 0.0 1.4796122986326825 4.982027336383995E-4 82-83 0.002740115035011197 1.4946082009151983E-4 0.0 1.6524388269318435 5.480230070022394E-4 84-85 0.002839755581738877 1.4946082009151983E-4 0.0 1.8244682308571827 5.480230070022394E-4 86-87 0.0029393961284665568 1.4946082009151983E-4 0.0 2.0095754565405297 5.480230070022394E-4 88-89 0.0029393961284665568 1.4946082009151983E-4 0.0 2.215308275396507 5.480230070022394E-4 90-91 0.0032881380420134365 1.4946082009151983E-4 0.0 2.457235522851314 5.480230070022394E-4 92-93 0.0033628684520591963 1.4946082009151983E-4 0.0 2.713785020538408 5.480230070022394E-4 94-95 0.0033877785887411163 1.4946082009151983E-4 0.0 2.9780815707335786 5.480230070022394E-4 96-97 0.0033877785887411163 1.4946082009151983E-4 0.0 3.2391398031601 5.480230070022394E-4 98-99 0.003562149545514556 1.4946082009151983E-4 0.0 3.5326310335464814 5.480230070022394E-4 100-101 0.003811250912333756 1.4946082009151983E-4 0.0 3.870661588320135 5.480230070022394E-4 102-103 0.004060352279152956 1.4946082009151983E-4 0.0 4.237139519184542 6.974838270937592E-4 104-105 0.004384184056017915 1.4946082009151983E-4 0.0 4.610417917363113 7.473041004575992E-4 106-107 0.004483824602745596 1.743709567734398E-4 0.0 4.9788637490253915 7.722142371395192E-4 108-109 0.004558555012791355 1.992810934553598E-4 0.0 5.361732549826501 7.971243738214392E-4 110-111 0.004583465149473275 2.2419123013727978E-4 0.0 5.803613464427079 8.469446471852791E-4 112-113 0.004583465149473275 2.9892164018303967E-4 0.0 6.289610231091338 8.469446471852791E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4050 0.0 61.36812 1 GTATCAA 7080 0.0 50.847015 1 ATCAACG 8995 0.0 40.123367 3 TCAACGC 9050 0.0 39.550484 4 CAACGCA 9185 0.0 38.80176 5 TATCAAC 9285 0.0 38.421192 2 AACGCAG 9310 0.0 38.02516 6 GTACATG 9425 0.0 31.745642 1 ACGCAGA 11265 0.0 31.161947 7 CGCAGAG 11390 0.0 30.611012 8 TACATGG 9725 0.0 30.191397 2 ACATGGG 9935 0.0 28.5941 3 GCAGAGT 12230 0.0 27.4869 9 TGGTATC 2220 0.0 25.753971 2 GAGTACT 6545 0.0 25.72585 12-13 GTGGTAT 2210 0.0 25.620825 1 CATGGGG 4785 0.0 24.51942 4 AGTACTT 6965 0.0 24.516232 12-13 CAGAGTA 11905 0.0 24.012983 10-11 AGAGTAC 11525 0.0 23.694847 10-11 >>END_MODULE