FastQCFastQC Report
Thu 26 May 2016
SRR1364558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817805
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40240.22136587807823171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28870.15881791501288642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.051.2041321
GTATCAA69300.048.8516961
TCAACGC84650.039.3595124
ATCAACG86000.039.156753
CAACGCA85800.038.5545965
TATCAAC88200.038.4498752
AACGCAG86900.038.2677466
GTACATG104950.035.0353621
TACATGG108550.033.1051642
ACGCAGA103600.031.8119937
CGCAGAG103400.031.129878
ACATGGG112150.031.0875853
TACCTGG15500.027.6369062
GCAGAGT108950.027.6327389
CATGGGG44900.027.5616784
CATGGGA68350.026.0268334
GAGTACT45250.025.64626712-13
GTACTTT49000.023.68354214-15
AGTACTT47050.023.27374812-13
CAGAGTA107500.022.8339910-11
Produced by FastQC (version 0.11.5)