FastQCFastQC Report
Thu 26 May 2016
SRR1364558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817805
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68030.374242561770927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58730.323081958735948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34360.1890191742238579No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19020.10463168491669898No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50650.057.0888331
GTATCAA87350.049.142641
ATCAACG112300.038.033763
TCAACGC113850.037.5682754
CAACGCA114750.037.1787075
TATCAAC114400.037.12732
AACGCAG118000.036.4572686
GTACATG102100.035.0943831
TACATGG105350.033.757452
ACATGGG111150.031.2456073
ACGCAGA138950.030.5750717
CGCAGAG138800.030.3508998
GCAGAGT146450.028.0318459
GAGTACT80900.027.80015412-13
TATAACG1556.771761E-626.9027232
GTACCGT2006.118535E-826.775676
AGTACTT83650.026.63727412-13
CATGGGA71200.026.0201784
CATGGGG43950.025.8883514
GTACTTT88350.025.72531514-15
Produced by FastQC (version 0.11.5)