##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364558_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1817805 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.88521651112193 31.0 30.0 34.0 26.0 34.0 2 29.71749830152299 31.0 30.0 34.0 25.0 34.0 3 29.98815384488435 31.0 30.0 34.0 26.0 34.0 4 33.51085622495262 35.0 33.0 37.0 28.0 37.0 5 32.917842122779945 35.0 33.0 37.0 26.0 37.0 6 32.93195914853354 35.0 33.0 37.0 26.0 37.0 7 32.698177747338136 35.0 33.0 37.0 25.0 37.0 8 32.61733134192061 35.0 33.0 37.0 25.0 37.0 9 33.79126638995932 38.0 33.0 39.0 23.0 39.0 10-11 33.732157189577535 38.0 33.0 39.0 23.0 39.0 12-13 33.60973261708489 37.5 33.0 39.0 21.5 39.0 14-15 34.53245067540247 38.0 33.0 41.0 20.5 41.0 16-17 34.54510934891256 38.5 33.0 41.0 22.0 41.0 18-19 34.5779932390988 38.5 33.5 41.0 22.0 41.0 20-21 34.61301872313037 39.0 34.0 41.0 22.0 41.0 22-23 34.58529187674145 39.0 34.0 41.0 22.0 41.0 24-25 34.55085831538587 39.0 33.5 41.0 22.5 41.0 26-27 34.43400997356702 39.0 33.0 41.0 22.0 41.0 28-29 34.28628620781657 38.5 33.0 41.0 21.5 41.0 30-31 34.10899381396794 38.0 33.0 40.0 20.0 41.0 32-33 33.88904778015244 38.0 33.0 40.0 18.5 41.0 34-35 33.69724750454532 38.0 33.0 40.0 17.5 41.0 36-37 33.4827830817937 38.0 32.5 40.0 16.0 41.0 38-39 33.31451998426674 38.0 32.0 40.0 15.0 41.0 40-41 33.32998478934759 38.0 32.0 40.0 15.0 41.0 42-43 33.18497198544398 38.0 32.0 40.0 11.0 41.0 44-45 33.00165446788847 38.0 32.0 40.0 8.5 41.0 46-47 32.711794994512616 38.0 31.5 40.0 4.5 41.0 48-49 32.39208303420884 38.0 31.0 40.0 2.0 41.0 50-51 32.20922431173861 38.0 31.0 40.0 2.0 41.0 52-53 32.0196291681451 37.5 30.0 40.0 2.0 41.0 54-55 31.832350829709455 37.0 30.0 40.0 2.0 41.0 56-57 31.612088205280543 37.0 30.0 40.0 2.0 41.0 58-59 31.40000302562706 36.5 30.0 40.0 2.0 41.0 60-61 31.16651786082666 36.0 29.5 40.0 2.0 41.0 62-63 30.989918610632053 36.0 29.5 39.5 2.0 41.0 64-65 30.84791162968525 35.5 29.5 39.0 2.0 41.0 66-67 30.54675418980584 35.0 29.0 39.0 2.0 41.0 68-69 30.264785551805613 35.0 29.0 39.0 2.0 40.5 70-71 29.949291040568156 35.0 29.0 38.0 2.0 40.0 72-73 29.655897084670798 35.0 29.0 37.0 2.0 40.0 74-75 29.303194512062625 35.0 29.0 37.0 2.0 39.0 76-77 28.994029062523207 34.5 28.5 36.0 2.0 39.0 78-79 28.70615275015747 34.0 28.0 36.0 2.0 38.5 80-81 28.425964556154263 34.0 28.0 35.5 2.0 37.0 82-83 28.146022538171035 34.0 27.5 35.0 2.0 37.0 84-85 27.925275538355322 34.0 27.0 35.0 2.0 36.5 86-87 27.712880094399566 34.0 27.0 35.0 2.0 36.0 88-89 27.53532034514153 34.0 26.5 35.0 2.0 36.0 90-91 27.392096787059117 34.0 26.0 35.0 2.0 35.5 92-93 27.276346747863496 34.0 26.0 35.0 2.0 35.0 94-95 27.163058468867675 34.0 26.0 35.0 2.0 35.0 96-97 27.04569494527741 34.0 26.0 35.0 2.0 35.0 98-99 26.96003999328861 34.0 25.5 35.0 2.0 35.0 100-101 26.856857033620216 34.0 25.0 35.0 2.0 35.0 102-103 25.600829571928784 31.5 23.5 34.0 2.0 35.0 104-105 26.520260423972868 33.0 25.0 35.0 2.0 35.0 106-107 26.679322864663703 34.0 25.0 35.0 2.0 35.0 108-109 26.68183000926942 34.0 25.0 35.0 2.0 35.0 110-111 26.60145367627441 34.0 25.0 35.0 2.0 35.0 112-113 26.50972601571676 34.0 25.0 35.0 2.0 35.0 114-115 26.40567662648084 34.0 25.0 35.0 2.0 35.0 116-117 26.280642863233403 34.0 24.5 35.0 2.0 35.0 118-119 26.141538008752313 34.0 24.0 35.0 2.0 35.0 120-121 25.940203432161315 34.0 23.5 35.0 2.0 35.0 122-123 25.644180481404774 33.0 21.5 35.0 2.0 35.0 124-125 23.858460891019664 31.0 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.688988115296695 1101 2 0.8181346111046892 1101 3 0.6008503388716484 1101 4 0.42508645351407637 1101 5 0.6218921808350117 1101 6 0.7256033574328811 1101 7 0.7584269704962026 1101 8 0.772968581384248 1101 9 0.9030025206281138 1101 10-11 0.9948919492639092 1101 12-13 0.8477253379260716 1101 14-15 0.9938671240774255 1101 16-17 1.0864778684983207 1101 18-19 1.0914129018646364 1101 20-21 1.1549259664699605 1101 22-23 1.1145079224789214 1101 24-25 1.0513387752645258 1101 26-27 1.0446914722816487 1101 28-29 1.192589459065367 1101 30-31 1.327856196286433 1101 32-33 1.2603891529003377 1101 34-35 1.3024559220403518 1101 36-37 1.3734040858263157 1101 38-39 1.2756521501372333 1101 40-41 1.067395294848538 1101 42-43 1.1665071154668922 1101 44-45 1.1277702336457835 1101 46-47 1.1805376805247505 1101 48-49 1.2840403076810105 1101 50-51 1.2629212569089745 1101 52-53 1.3359590894938052 1101 54-55 1.261902924419875 1101 56-57 1.2937398631024308 1101 58-59 1.250086919457484 1101 60-61 1.2459052920552693 1101 62-63 1.270066578204922 1101 64-65 1.1982429613771686 1101 66-67 1.2031789872262948 1101 68-69 1.2764658864606453 1101 70-71 1.2075848310881518 1101 72-73 1.103222796499022 1101 74-75 1.1866203910247357 1101 76-77 1.2210381996561637 1101 78-79 1.2046065804478836 1101 80-81 0.9875304991663292 1101 82-83 1.0838169893898382 1101 84-85 1.0724563844061734 1101 86-87 1.1758444009965245 1101 88-89 1.1886919662249156 1101 90-91 1.136437434639891 1101 92-93 1.1353984028960404 1101 94-95 1.087315902011241 1101 96-97 1.12386501343601 1101 98-99 1.0827914292308591 1101 100-101 1.0809668792054055 1101 102-103 0.9675989503695419 1101 104-105 0.9958913854903386 1101 106-107 1.0706491296180118 1101 108-109 1.122065729670581 1101 110-111 1.1027987220568534 1101 112-113 0.9914958197096979 1101 114-115 1.099891274148888 1101 116-117 0.9961929150528519 1101 118-119 1.084402672800902 1101 120-121 1.0702248503858804 1101 122-123 1.058558999678013 1101 124-125 1.3200643815638422 1102 1 0.27002324732806215 1102 2 -0.3710628532688105 1102 3 0.15972347668965625 1102 4 0.28078137881193044 1102 5 0.36269739661261724 1102 6 0.35049868170006704 1102 7 0.39088382656586873 1102 8 0.4487682587483377 1102 9 0.8768652516568594 1102 10-11 0.9840660329943276 1102 12-13 0.8714486332453646 1102 14-15 1.0895101398241636 1102 16-17 1.0487944633267574 1102 18-19 1.0281823981262832 1102 20-21 0.8581956377330329 1102 22-23 1.032848057967911 1102 24-25 1.0205356968226056 1102 26-27 1.0827755572164293 1102 28-29 0.8090192659644373 1102 30-31 1.0645237167565185 1102 32-33 1.190835202985241 1102 34-35 1.1142560930272083 1102 36-37 0.7495635763035793 1102 38-39 0.3340634707788581 1102 40-41 0.018841657617727492 1102 42-43 0.43916852813270424 1102 44-45 0.0838998636209567 1102 46-47 0.2617832242081022 1102 48-49 0.015308652438910997 1102 50-51 -0.08189566162970152 1102 52-53 -0.34235930793416003 1102 54-55 -0.2443658868200238 1102 56-57 -0.30534140767368356 1102 58-59 -0.24178555058448126 1102 60-61 -0.2630441650444979 1102 62-63 -0.20939838956259038 1102 64-65 -0.30062949509440173 1102 66-67 -0.3349147100647727 1102 68-69 -0.04271028371650942 1102 70-71 -0.5831280212541827 1102 72-73 -0.30837695685922384 1102 74-75 -0.19040597849572904 1102 76-77 -0.5688222628564965 1102 78-79 -0.2611233196910412 1102 80-81 -0.418818504569483 1102 82-83 -0.6120062863089366 1102 84-85 -0.46939567351449085 1102 86-87 -0.3062155495733485 1102 88-89 -0.3210187046779751 1102 90-91 -0.3633236700347098 1102 92-93 -0.3271782909849037 1102 94-95 -0.17905040679305628 1102 96-97 -0.3327788776005782 1102 98-99 -0.42876417796755106 1102 100-101 -0.2985270387175092 1102 102-103 -0.23837405474140283 1102 104-105 -0.260575751277095 1102 106-107 -0.18169840568901918 1102 108-109 -0.24268092537078445 1102 110-111 -0.14046548995327868 1102 112-113 0.09777102033936202 1102 114-115 0.021634042405722198 1102 116-117 -0.023547048092240175 1102 118-119 0.20090549120030587 1102 120-121 0.41023806454861145 1102 122-123 0.33403485372173236 1102 124-125 0.5288432052036818 1103 1 0.35810131130045875 1103 2 -0.23966762075639636 1103 3 0.21864184135570852 1103 4 0.15622099096977138 1103 5 0.33318974670849144 1103 6 0.44845397103594564 1103 7 0.5351429309557076 1103 8 0.6300554586673996 1103 9 0.8859456899052915 1103 10-11 0.778447618534365 1103 12-13 0.814055056955219 1103 14-15 0.7808909407116218 1103 16-17 0.7263774568109369 1103 18-19 0.7462475247141214 1103 20-21 0.7952806385648046 1103 22-23 0.7897959583626033 1103 24-25 0.7251571044196439 1103 26-27 0.8076240978204723 1103 28-29 0.9346430478375112 1103 30-31 0.8174975585191362 1103 32-33 0.8912428244296677 1103 34-35 0.855513269861035 1103 36-37 0.8481332820539222 1103 38-39 0.8696354932367711 1103 40-41 0.7357343910518779 1103 42-43 0.8664433257153306 1103 44-45 0.8695251862971602 1103 46-47 0.9091828437764775 1103 48-49 0.7497973717704767 1103 50-51 0.7063141748744357 1103 52-53 0.639577456207121 1103 54-55 0.643889059094537 1103 56-57 0.74807712229007 1103 58-59 0.8201450700981461 1103 60-61 0.7718650439774422 1103 62-63 0.5777211735153109 1103 64-65 0.5564871881490667 1103 66-67 0.6561430583415095 1103 68-69 0.7131462850080368 1103 70-71 0.5196454526064187 1103 72-73 0.6001300966186385 1103 74-75 0.6679197836903406 1103 76-77 0.6601547694409646 1103 78-79 0.5532301504247847 1103 80-81 0.6349639907499025 1103 82-83 0.6813845712820523 1103 84-85 0.7129362953426615 1103 86-87 0.7174088991344867 1103 88-89 0.7052943185089973 1103 90-91 0.7583646438342271 1103 92-93 0.7188893945778148 1103 94-95 0.7405267245842602 1103 96-97 0.8015829653410655 1103 98-99 0.8017679790312577 1103 100-101 0.7334692481162577 1103 102-103 0.5024118508042612 1103 104-105 0.5659849150948624 1103 106-107 0.668163239502114 1103 108-109 0.7269821142471855 1103 110-111 0.6309659598987309 1103 112-113 0.7050767412200543 1103 114-115 0.8156597052438492 1103 116-117 0.8017685590441594 1103 118-119 0.6987885800816507 1103 120-121 0.5959717231814174 1103 122-123 0.5901760900582147 1103 124-125 0.7945351633824451 1104 1 0.32495072117438895 1104 2 0.48241904645860245 1104 3 0.24191010247128375 1104 4 0.025003192365495863 1104 5 0.292336516307806 1104 6 0.4565894630102747 1104 7 0.4086360508812561 1104 8 0.3999133354479696 1104 9 0.3350906467871724 1104 10-11 0.37565093498880486 1104 12-13 0.4058210261438546 1104 14-15 0.630521534122316 1104 16-17 0.5483967140154178 1104 18-19 0.46587689309815516 1104 20-21 0.4300775813383524 1104 22-23 0.4930892003614602 1104 24-25 0.572609485030199 1104 26-27 0.46452317829908907 1104 28-29 0.6891438132443639 1104 30-31 0.568362892934644 1104 32-33 0.7582798659889249 1104 34-35 0.8563595848318926 1104 36-37 0.8608630265466317 1104 38-39 0.9363159218860773 1104 40-41 0.8825019777319056 1104 42-43 0.9319323387825946 1104 44-45 0.8287768144219996 1104 46-47 0.7693922066029444 1104 48-49 0.6445937456378772 1104 50-51 0.6375191999257623 1104 52-53 0.8128189693418051 1104 54-55 0.4675844425956477 1104 56-57 0.6641320024112147 1104 58-59 0.834987750778506 1104 60-61 0.8066822339058177 1104 62-63 0.8704053357453354 1104 64-65 0.8573450825066828 1104 66-67 0.9071681963448555 1104 68-69 0.7470192115028134 1104 70-71 0.3512167646555966 1104 72-73 0.35001299453527324 1104 74-75 0.7380493399878283 1104 76-77 0.8817444488395481 1104 78-79 0.9166355850080272 1104 80-81 0.8394105423535123 1104 82-83 0.8427941784698021 1104 84-85 0.76773926530106 1104 86-87 0.6969360603102928 1104 88-89 0.7085861248249046 1104 90-91 0.7216205527094175 1104 92-93 0.6800654416435243 1104 94-95 0.6250519945496755 1104 96-97 0.6152927714178702 1104 98-99 0.6863878430600039 1104 100-101 0.6802896732957713 1104 102-103 0.581771842131122 1104 104-105 0.682159909550041 1104 106-107 0.7407561996374952 1104 108-109 0.7466148101954317 1104 110-111 0.714814741426558 1104 112-113 0.7041678331562231 1104 114-115 0.6252537303339523 1104 116-117 0.6619865091331114 1104 118-119 0.5974465385485495 1104 120-121 0.6063539247918293 1104 122-123 0.6319007263373351 1104 124-125 1.1288754650556605 1105 1 -0.02841614330760578 1105 2 -0.2991442761704839 1105 3 -0.07353482694178481 1105 4 -0.045231743583485695 1105 5 -0.1253173219739807 1105 6 0.20752302649760423 1105 7 0.18594561535999787 1105 8 0.4969445815000455 1105 9 0.5296384194496753 1105 10-11 0.3910110673059606 1105 12-13 0.42159952912418674 1105 14-15 0.5703052353531248 1105 16-17 0.5250897449187022 1105 18-19 0.5346882057232492 1105 20-21 0.5132989859809243 1105 22-23 0.4862067723437349 1105 24-25 0.34343467186654664 1105 26-27 0.28661531502949344 1105 28-29 0.4166466811455152 1105 30-31 0.42668563498637013 1105 32-33 0.5135103682382578 1105 34-35 0.418878158844997 1105 36-37 0.5583649947403089 1105 38-39 0.5509137894055556 1105 40-41 0.3858298259243753 1105 42-43 0.35996376143402387 1105 44-45 0.313877498106109 1105 46-47 0.18142046677588297 1105 48-49 0.13166830465086576 1105 50-51 0.021430866030279105 1105 52-53 0.011834798979720063 1105 54-55 0.059756332445246585 1105 56-57 0.03530946550081637 1105 58-59 -0.23619782705761594 1105 60-61 -0.03794556994297693 1105 62-63 0.07082166101111298 1105 64-65 -0.25413181606660373 1105 66-67 -0.026314678264661495 1105 68-69 0.11805129095407096 1105 70-71 0.03483576514074116 1105 72-73 0.12282121419865533 1105 74-75 -0.003084564158431391 1105 76-77 0.056579403317421395 1105 78-79 0.0029567693492644764 1105 80-81 0.030260691059250888 1105 82-83 0.07588920295374635 1105 84-85 0.03817709665042557 1105 86-87 0.11625642956735405 1105 88-89 -0.005567450295792753 1105 90-91 0.05290989235220067 1105 92-93 0.09178421848519136 1105 94-95 0.060604330052601796 1105 96-97 -0.052120064239527863 1105 98-99 -0.22087377222374016 1105 100-101 -0.06195502869049463 1105 102-103 -0.24031882405213523 1105 104-105 -0.1894478891090614 1105 106-107 -0.09516028344473426 1105 108-109 -0.11252702661688119 1105 110-111 -0.18303363420037755 1105 112-113 -0.34782984498351865 1105 114-115 -0.33603565342524533 1105 116-117 -0.4403723287014998 1105 118-119 -0.2735829340236755 1105 120-121 -0.106011130783493 1105 122-123 -0.09976552790769944 1105 124-125 0.3029020196612855 1106 1 -0.029699896910130974 1106 2 -0.38097530776257216 1106 3 0.19703898685071408 1106 4 0.012022769644644882 1106 5 0.25331778727969123 1106 6 0.38893939792541943 1106 7 0.5227728634075461 1106 8 0.3709862025296218 1106 9 0.5162893400441888 1106 10-11 0.5214011905210185 1106 12-13 0.5265381255617356 1106 14-15 0.5818200605248123 1106 16-17 0.4578258697124369 1106 18-19 0.6109507979917552 1106 20-21 0.5580851392277353 1106 22-23 0.48336595248737524 1106 24-25 0.5943809659305685 1106 26-27 0.6255717502166576 1106 28-29 0.7486411904167269 1106 30-31 0.741523248518206 1106 32-33 0.5358165559143089 1106 34-35 0.6792196772803436 1106 36-37 0.748942031174515 1106 38-39 0.6752989889135961 1106 40-41 0.6113060324263699 1106 42-43 0.38908359271444937 1106 44-45 0.49326584886821934 1106 46-47 0.6603564239106703 1106 48-49 0.7573743793304928 1106 50-51 0.8885635919568386 1106 52-53 0.7009567514926687 1106 54-55 0.7037527345085515 1106 56-57 0.14438650499449324 1106 58-59 0.6235797290785534 1106 60-61 0.7162276490885588 1106 62-63 0.5549838047613314 1106 64-65 0.6633236839425756 1106 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-1.0636616086694843 2216 62-63 -1.3799827787205885 2216 64-65 -1.301723586998122 2216 66-67 -1.2090747617655673 2216 68-69 -1.0700246651480363 2216 70-71 -1.0886713640609358 2216 72-73 -1.0559825512624634 2216 74-75 -0.9672483037169677 2216 76-77 -1.0580595497782 2216 78-79 -1.0670709330371935 2216 80-81 -1.0090716075860122 2216 82-83 -0.9379449851442381 2216 84-85 -1.0275375793829866 2216 86-87 -1.023029007956989 2216 88-89 -1.0413040195801173 2216 90-91 -1.0369796895623757 2216 92-93 -1.0759310599179166 2216 94-95 -1.0176632145353643 2216 96-97 -1.0432910912387676 2216 98-99 -1.146416004810341 2216 100-101 -1.0998703661892861 2216 102-103 -1.0283627557252863 2216 104-105 -0.9923555611738877 2216 106-107 -1.110025906955002 2216 108-109 -1.1111017340972325 2216 110-111 -1.1525944487165063 2216 112-113 -1.0654214024949304 2216 114-115 -1.1847149767031837 2216 116-117 -1.2721679530414818 2216 118-119 -1.3066200242699075 2216 120-121 -1.4399328530814763 2216 122-123 -1.4131856809972518 2216 124-125 -1.7596722422617894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66616.0 3 21056.0 4 9816.0 5 7796.0 6 8527.0 7 9972.0 8 11186.0 9 12344.0 10 13825.0 11 14659.0 12 16438.0 13 19182.0 14 22723.0 15 19663.0 16 13101.0 17 11328.0 18 10467.0 19 9714.0 20 9491.0 21 9900.0 22 11233.0 23 13759.0 24 17998.0 25 23459.0 26 29472.0 27 32312.0 28 36706.0 29 43491.0 30 50250.0 31 58720.0 32 70971.0 33 87990.0 34 113931.0 35 154668.0 36 247401.0 37 375889.0 38 131290.0 39 461.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20937078254492 19.736987731490277 14.051383366563645 23.002258119401162 2 15.45891886093393 20.986904535965078 39.35757685780378 24.196599745297213 3 19.157060300747332 26.2683291112083 29.330483742755685 25.244126845288683 4 12.620447128817991 17.490866999056138 36.62330625097057 33.2653796211553 5 13.063172342467977 39.054464037671806 33.51619123063255 14.36617238922767 6 31.05833684031015 37.53246360308174 17.198764443931005 14.210435112677104 7 27.849475201994554 33.6007706242948 20.467149718855264 18.082604454855378 8 26.327073470634026 35.76907736023965 19.16414740890098 18.739701760225344 9 26.912990305971725 16.082261430134615 19.689148530821903 37.31559973307176 10-11 24.98782862037343 27.564117727635058 26.51622383015851 20.931829821833006 12-13 26.032495234637377 24.84012311551569 27.497118777866714 21.630262871980218 14-15 23.02990311386706 25.561226622892235 25.975325172570862 25.43354509066984 16-17 22.106077330438254 29.257070304624307 26.391925608834654 22.244926756102785 18-19 21.884281689706942 26.95918435832253 29.667766739155187 21.48876721281534 20-21 22.757817128780246 26.101802702320587 29.82639177322116 21.313988395678006 22-23 22.543067050646247 26.10890607078317 29.520658156402913 21.82736872216767 24-25 22.203069086068087 26.260324952346377 29.670344178831066 21.866261782754474 26-27 21.975761976669663 26.54811709726841 29.577704979356973 21.898415946704954 28-29 21.78495493190964 26.31651909858318 29.926367239610407 21.97215872989677 30-31 21.959011005030792 26.159956651015925 29.883843426550154 21.997188917403133 32-33 21.842469351773154 26.312475760601384 29.73349726730865 22.11155762031681 34-35 22.334628851829542 26.22319225659518 29.52107074191126 21.92110814966402 36-37 22.251094039239632 26.105935455123074 29.359337222639393 22.2836332829979 38-39 22.13383723776753 26.315501387662593 29.549786693292184 22.000874681277697 40-41 22.180486905911252 26.053812152568618 29.716636272867554 22.04906466865258 42-43 22.165303759204093 26.208504212498042 29.247526549877463 22.378665478420405 44-45 22.26333407598725 26.4197479927715 28.936739639290245 22.380178291951005 46-47 22.342000379578668 26.720165254248947 28.301935576148157 22.635898790024235 48-49 22.691597833651027 26.822046369109998 27.936632367058074 22.549723430180904 50-51 22.45684285631376 26.977048411971694 27.875577379195615 22.69053135251893 52-53 22.852863756013434 26.966203745726304 27.61990422515066 22.561028273109603 54-55 22.481536798501487 26.932344228341325 27.76527185259145 22.820847120565737 56-57 22.529780505624903 27.314577428214292 27.5553027235436 22.600339342617207 58-59 22.61154503335376 26.82620506484262 27.69987776338909 22.862372138414532 60-61 22.68070557623067 26.860636866990685 27.99964792703288 22.459009629745765 62-63 22.58044729770245 26.882861473040286 27.975085336435978 22.561605892821287 64-65 22.412137538896637 26.69074707675246 27.915662819613946 22.98145256473696 66-67 22.427570614009753 27.202972816116137 27.600127626450583 22.769328943423524 68-69 22.375309782952517 27.005344357618117 27.840609966415542 22.778735893013828 70-71 22.25720030476316 27.036205753642445 27.773606079860052 22.932987861734343 72-73 22.565814265006424 26.977783645660562 27.53826730589915 22.918134783433867 74-75 22.44448319738935 27.11547527950256 27.409899861534626 23.030141661573467 76-77 22.600141379300858 27.002813833166922 27.602080531190087 22.794964256342126 78-79 22.522685326533924 27.12155594246908 27.477727258974422 22.878031472022577 80-81 22.23373489866614 27.363058593257904 27.479297811699066 22.92390869637689 82-83 22.496087314095845 27.295501992788008 27.24395630994524 22.964454383170914 84-85 22.421684394090676 27.09457285022321 27.171726340284025 23.31201641540209 86-87 22.42047414326619 26.917326115837508 27.605271192454634 23.056928548441665 88-89 22.519285961448432 26.943848324295715 27.378446043592902 23.15841967066295 90-91 22.32035339324075 27.279713720668607 27.472473670168142 22.9274592159225 92-93 22.376447107545314 27.04281193818339 27.449025286010727 23.131715668260565 94-95 22.326707210069287 26.860554349889014 27.392349564447233 23.420388875594465 96-97 22.541538625743453 26.800525193584672 27.348330821342575 23.309605359329296 98-99 22.222402546050198 26.760043846197224 27.300501865663946 23.717051742088636 100-101 22.06372372924154 26.822978699526285 27.553138407693456 23.56015916353872 102-103 22.491906447611267 26.73397311592828 27.284664746768765 23.489455689691688 104-105 22.368020772305062 26.686938367976765 27.5143648521156 23.430676007602575 106-107 22.183004547593775 26.81248416676546 27.46432519545191 23.540186090188854 108-109 22.27010047832413 26.904838527784886 27.039231380703647 23.785829613187335 110-111 22.01733797667127 26.874028874989925 27.413801781987747 23.694831366351057 112-113 22.256705202153146 26.714031483024858 27.412153668847868 23.61710964597413 114-115 21.977082250296373 26.987823226363666 27.151454638973927 23.883639884366033 116-117 21.782506924560117 26.858656456550623 27.31332568674858 24.045510932140687 118-119 21.990752583472926 27.01304595377392 26.981524420936235 24.014677041816917 120-121 21.75025243655254 26.894484409171938 27.254015673483668 24.101247480791855 122-123 22.011929862202802 26.928479967211445 26.983769390839836 24.075820779745914 124-125 21.83574693023512 26.86977589339794 27.01943725565773 24.27503992070921 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 336.0 1 257.0 2 296.0 3 456.0 4 359.5 5 278.5 6 545.5 7 1315.5 8 2217.5 9 2606.5 10 2163.0 11 1471.0 12 1296.0 13 1400.0 14 1563.0 15 1756.5 16 1990.0 17 2280.5 18 2626.5 19 2958.0 20 3347.5 21 3885.5 22 4641.0 23 5445.0 24 6349.5 25 7828.5 26 10232.5 27 12735.5 28 14861.5 29 17301.5 30 20373.5 31 23799.5 32 26950.5 33 30817.5 34 35937.5 35 39570.5 36 43314.0 37 47933.5 38 50632.5 39 51850.5 40 52040.5 41 54031.0 42 56909.0 43 59049.0 44 61767.5 45 63975.0 46 64272.0 47 64182.5 48 68507.0 49 71382.0 50 70649.5 51 69104.0 52 65740.5 53 64346.5 54 62983.5 55 58158.5 56 52342.0 57 46924.5 58 42484.0 59 38463.0 60 33511.0 61 27302.5 62 21101.0 63 16089.0 64 12125.0 65 9127.0 66 6741.0 67 5256.5 68 3797.5 69 2525.5 70 1795.5 71 1325.0 72 1056.5 73 921.0 74 693.0 75 486.0 76 322.0 77 171.0 78 113.5 79 74.0 80 39.0 81 27.0 82 20.5 83 16.0 84 14.0 85 9.0 86 4.0 87 4.5 88 6.0 89 3.5 90 1.5 91 1.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09307929068299405 2 0.0 3 0.0 4 0.10248624027329664 5 0.0 6 0.0 7 0.0031906612645470774 8 0.00770159615580329 9 0.004841003297933496 10-11 8.801824178060903E-4 12-13 0.0 14-15 5.501140111288064E-5 16-17 4.675969094594855E-4 18-19 0.0025305244511925097 20-21 0.001870387637837942 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.750570055644032E-5 52-53 0.0 54-55 0.0 56-57 0.00951697239252835 58-59 0.0010452166211447323 60-61 0.0 62-63 0.0 64-65 3.5757410723372417E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.001210250824483374 76-77 0.0 78-79 0.0 80-81 1.1002280222576128E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0017603648356121805 90-91 0.0 92-93 0.0022554674456281066 94-95 0.0 96-97 0.010424660510890882 98-99 0.004813497597377056 100-101 0.0028605928578697933 102-103 0.0 104-105 0.0 106-107 0.0016778477339428597 108-109 0.0 110-111 1.9253990389508224E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0011827451239269338 122-123 0.005363611608505863 124-125 0.0937944388974615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1817805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.12354543157758 #Duplication Level Percentage of deduplicated Percentage of total 1 77.17992996039312 41.00071515656919 2 11.960337615520734 12.707510773902438 3 4.368579138658584 6.962232370319139 4 2.031012482677683 4.315783355825163 5 1.1592482638966393 3.0791688906795276 6 0.7152603076547079 2.2798298069459424 7 0.4892495803803086 1.8193470617499514 8 0.3304058730022744 1.40418651242371 9 0.2462440337037154 1.1773220500542823 >10 1.347409541731695 13.18467599174894 >50 0.10539097424765655 3.873857924988605 >100 0.06267817457418368 5.8364160878116 >500 0.0035277517316431107 1.1697483970460671 >1k 5.18787019359281E-4 0.4905094234903851 >5k 2.0751480774371242E-4 0.6986961964450075 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6803 0.374242561770927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5873 0.323081958735948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3436 0.1890191742238579 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1902 0.10463168491669898 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.501140111288064E-5 0.0 5 0.0 0.0 0.0 5.501140111288064E-5 0.0 6 0.0 0.0 0.0 5.501140111288064E-5 0.0 7 0.0 0.0 0.0 5.501140111288064E-5 0.0 8 0.0 0.0 0.0 5.501140111288064E-5 0.0 9 0.0 0.0 0.0 5.501140111288064E-5 0.0 10-11 0.0 0.0 0.0 5.501140111288064E-5 0.0 12-13 0.0 0.0 0.0 5.501140111288064E-5 0.0 14-15 2.750570055644032E-5 0.0 0.0 1.1002280222576128E-4 0.0 16-17 5.501140111288064E-5 0.0 0.0 1.375285027822016E-4 0.0 18-19 5.501140111288064E-5 0.0 0.0 1.6503420333864192E-4 0.0 20-21 5.501140111288064E-5 0.0 0.0 1.6503420333864192E-4 0.0 22-23 5.501140111288064E-5 0.0 0.0 3.850798077901645E-4 0.0 24-25 5.501140111288064E-5 0.0 0.0 4.951026100159258E-4 0.0 26-27 5.501140111288064E-5 0.0 0.0 9.076881183625305E-4 0.0 28-29 5.501140111288064E-5 0.0 0.0 0.0017328591350557403 0.0 30-31 5.501140111288064E-5 0.0 0.0 0.003850798077901645 0.0 32-33 5.501140111288064E-5 0.0 0.0 0.008499261471940061 0.0 34-35 5.501140111288064E-5 0.0 0.0 0.013312759069317115 0.0 36-37 5.501140111288064E-5 0.0 0.0 0.024039982286328844 0.0 38-39 1.1002280222576128E-4 0.0 0.0 0.04348651257973215 0.0 40-41 1.1002280222576128E-4 0.0 0.0 0.06892928559443945 0.0 42-43 1.1002280222576128E-4 0.0 0.0 0.09294176218021186 0.0 44-45 1.1002280222576128E-4 0.0 0.0 0.1222353332728208 0.0 46-47 1.375285027822016E-4 0.0 0.0 0.15879040931233 0.0 48-49 1.6503420333864192E-4 0.0 0.0 0.19757344709691083 0.0 50-51 1.6503420333864192E-4 5.501140111288064E-5 0.0 0.2440030696361821 0.0 52-53 1.6503420333864192E-4 5.501140111288064E-5 0.0 0.2917804715027189 0.0 54-55 1.6503420333864192E-4 5.501140111288064E-5 0.0 0.3363672121047087 0.0 56-57 1.6503420333864192E-4 5.501140111288064E-5 0.0 0.39209376143205676 0.0 58-59 1.6503420333864192E-4 5.501140111288064E-5 0.0 0.46083050712260115 0.0 60-61 1.6503420333864192E-4 8.251710166932096E-5 0.0 0.5368012520594894 0.0 62-63 1.6503420333864192E-4 1.1002280222576128E-4 0.0 0.609306278726266 0.0 64-65 1.6503420333864192E-4 1.1002280222576128E-4 0.0 0.6825264536075102 0.0 66-67 2.475513050079629E-4 1.1002280222576128E-4 0.0 0.7659787490957501 0.0 68-69 2.750570055644032E-4 1.1002280222576128E-4 0.0 0.8716831563341503 0.0 70-71 3.5757410723372417E-4 1.1002280222576128E-4 0.0 0.9930658128897214 0.0 72-73 4.125855083466048E-4 1.1002280222576128E-4 0.0 1.1178316706137346 0.0 74-75 4.4009120890304513E-4 1.1002280222576128E-4 0.0 1.236353734311436 0.0 76-77 4.4009120890304513E-4 1.1002280222576128E-4 5.501140111288064E-5 1.3701689675185182 0.0 78-79 4.4009120890304513E-4 1.1002280222576128E-4 5.501140111288064E-5 1.5291794224352997 0.0 80-81 5.501140111288064E-4 1.1002280222576128E-4 5.501140111288064E-5 1.7128074793500954 0.0 82-83 6.05125412241687E-4 1.1002280222576128E-4 5.501140111288064E-5 1.9085930559108375 0.0 84-85 6.326311127981274E-4 1.1002280222576128E-4 5.501140111288064E-5 2.101600556715379 0.0 86-87 6.601368133545677E-4 1.1002280222576128E-4 5.501140111288064E-5 2.304372581217457 0.0 88-89 6.601368133545677E-4 1.1002280222576128E-4 5.501140111288064E-5 2.5313221165086466 0.0 90-91 6.601368133545677E-4 1.1002280222576128E-4 5.501140111288064E-5 2.7946341879354497 0.0 92-93 7.70159615580329E-4 1.1002280222576128E-4 5.501140111288064E-5 3.075714941921713 0.0 94-95 7.70159615580329E-4 1.1002280222576128E-4 5.501140111288064E-5 3.3599588514719674 0.0 96-97 7.70159615580329E-4 1.1002280222576128E-4 5.501140111288064E-5 3.6538572619175325 0.0 98-99 7.70159615580329E-4 1.1002280222576128E-4 5.501140111288064E-5 3.9686049933848793 0.0 100-101 7.70159615580329E-4 1.1002280222576128E-4 5.501140111288064E-5 4.337181380841179 0.0 102-103 7.70159615580329E-4 1.1002280222576128E-4 5.501140111288064E-5 4.744760851686512 0.0 104-105 8.801824178060903E-4 1.1002280222576128E-4 5.501140111288064E-5 5.136001936401319 0.0 106-107 9.35193818918971E-4 1.1002280222576128E-4 5.501140111288064E-5 5.537640175926461 0.0 108-109 9.35193818918971E-4 1.1002280222576128E-4 5.501140111288064E-5 5.946539920398502 0.0 110-111 9.35193818918971E-4 1.1002280222576128E-4 5.501140111288064E-5 6.419555452867607 0.0 112-113 9.35193818918971E-4 1.1002280222576128E-4 5.501140111288064E-5 6.935287338300863 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5065 0.0 57.088833 1 GTATCAA 8735 0.0 49.14264 1 ATCAACG 11230 0.0 38.03376 3 TCAACGC 11385 0.0 37.568275 4 CAACGCA 11475 0.0 37.178707 5 TATCAAC 11440 0.0 37.1273 2 AACGCAG 11800 0.0 36.457268 6 GTACATG 10210 0.0 35.094383 1 TACATGG 10535 0.0 33.75745 2 ACATGGG 11115 0.0 31.245607 3 ACGCAGA 13895 0.0 30.575071 7 CGCAGAG 13880 0.0 30.350899 8 GCAGAGT 14645 0.0 28.031845 9 GAGTACT 8090 0.0 27.800154 12-13 TATAACG 155 6.771761E-6 26.902723 2 GTACCGT 200 6.118535E-8 26.77567 6 AGTACTT 8365 0.0 26.637274 12-13 CATGGGA 7120 0.0 26.020178 4 CATGGGG 4395 0.0 25.888351 4 GTACTTT 8835 0.0 25.725315 14-15 >>END_MODULE