FastQCFastQC Report
Thu 26 May 2016
SRR1364557_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364557_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375518
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43100.18143411247567898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31630.1331499066729867No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49300.047.4276961
GTATCAA80900.046.920061
ATCAACG93750.039.72653
TCAACGC94250.039.073884
CAACGCA94750.038.8676875
AACGCAG98650.037.508876
TATCAAC102300.036.522562
GTACATG123950.033.6478961
ACGCAGA112350.032.6173257
TACATGG130700.031.1356372
CGCAGAG114550.030.853558
ACATGGG129650.030.4700223
GCAGAGT122500.027.5889519
CATGGGG48850.027.0374474
CATGGGA83800.025.3455354
GAGTACT51950.023.36952412-13
CAGAGTA121600.022.80476410-11
AGAGTAC115150.022.402610-11
ATGGGAT38150.022.3007135
GTACTTT55550.021.53363214-15
Produced by FastQC (version 0.11.5)