FastQCFastQC Report
Thu 26 May 2016
SRR1364557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375518
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77690.3270444593558121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65560.2759819121555804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.17053122729442588No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56700.054.6813741
GTATCAA95100.046.394911
ATCAACG117500.037.357843
TCAACGC118500.037.0425874
CAACGCA119800.036.4538355
AACGCAG123050.035.732786
TATCAAC124750.034.948032
ACGCAGA142500.030.5632957
CGCAGAG145350.030.0049538
GTACATG115750.029.8762531
TACATGG122400.026.9088952
GCAGAGT157550.026.6612879
GAGTACT89400.026.38763812-13
ACATGGG117850.026.3305723
GTACTTT98150.024.27767614-15
CATGGGG44400.024.1458054
CAGAGTA154700.023.3824410-11
AGAGTAC148800.023.13007210-11
AGTACTT98600.022.7790112-13
CATGGGA76600.021.2268734
Produced by FastQC (version 0.11.5)