##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364557_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2375518 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.95201846502531 31.0 30.0 34.0 26.0 34.0 2 29.80089437335352 31.0 30.0 34.0 26.0 34.0 3 30.079476560480703 31.0 30.0 34.0 26.0 34.0 4 33.61892648256086 35.0 35.0 37.0 28.0 37.0 5 33.04277467061921 35.0 33.0 37.0 26.0 37.0 6 33.06355498042953 35.0 33.0 37.0 26.0 37.0 7 32.83736978629503 35.0 33.0 37.0 25.0 37.0 8 32.75548280417156 35.0 33.0 37.0 25.0 37.0 9 33.95661072658679 38.0 33.0 39.0 25.0 39.0 10-11 33.89278927795959 38.0 33.0 39.0 24.5 39.0 12-13 33.784814932995666 38.0 33.0 39.0 23.0 39.0 14-15 34.71376200895973 39.0 33.0 41.0 23.0 41.0 16-17 34.715635916040206 39.0 33.0 41.0 23.0 41.0 18-19 34.746725766759084 39.0 33.5 41.0 23.0 41.0 20-21 34.7671255279901 39.0 34.0 41.0 23.0 41.0 22-23 34.734534951955744 39.0 34.0 41.0 23.0 41.0 24-25 34.707922230014674 39.0 34.0 41.0 23.5 41.0 26-27 34.58690736083666 39.0 33.5 41.0 23.0 41.0 28-29 34.44321175423634 39.0 33.0 41.0 22.5 41.0 30-31 34.29581611252787 38.0 33.0 40.5 22.0 41.0 32-33 34.09901756164339 38.0 33.0 40.0 20.0 41.0 34-35 33.92293575548575 38.0 33.0 40.0 19.0 41.0 36-37 33.74123117568463 38.0 33.0 40.0 17.5 41.0 38-39 33.58176742925122 38.0 33.0 40.0 16.0 41.0 40-41 33.62154696365172 38.0 33.0 40.0 16.0 41.0 42-43 33.50697700459436 38.0 33.0 40.0 15.0 41.0 44-45 33.34841706103679 38.0 32.0 40.0 13.5 41.0 46-47 33.1203385956242 38.0 32.0 40.0 9.0 41.0 48-49 32.84650337315904 38.0 32.0 40.0 7.5 41.0 50-51 32.69655607745342 38.0 31.0 40.0 2.0 41.0 52-53 32.52718207144716 38.0 31.0 40.0 2.0 41.0 54-55 32.359028430851716 38.0 31.0 40.0 2.0 41.0 56-57 32.1569910225896 37.5 31.0 40.0 2.0 41.0 58-59 31.969135784279473 37.0 31.0 40.0 2.0 41.0 60-61 31.755683392001238 37.0 30.0 40.0 2.0 41.0 62-63 31.588088366411032 36.0 30.0 40.0 2.0 41.0 64-65 31.459389909905966 36.0 30.0 40.0 2.0 41.0 66-67 31.16873393508279 36.0 30.0 39.0 2.0 41.0 68-69 30.889757518149725 35.0 30.0 39.0 2.0 41.0 70-71 30.580448348528616 35.0 29.5 39.0 2.0 40.5 72-73 30.28282947129847 35.0 29.0 38.0 2.0 40.0 74-75 29.917967786394378 35.0 29.0 37.0 2.0 39.5 76-77 29.59420997862361 35.0 29.0 37.0 2.0 39.0 78-79 29.293951045624574 35.0 29.0 36.0 2.0 39.0 80-81 28.99623050635693 34.0 29.0 36.0 2.0 38.0 82-83 28.685500173014894 34.0 29.0 35.5 2.0 37.0 84-85 28.43377065549493 34.0 28.5 35.0 2.0 37.0 86-87 28.20862607650205 34.0 28.0 35.0 2.0 36.0 88-89 28.01675718727452 34.0 28.0 35.0 2.0 36.0 90-91 27.85938856283135 34.0 27.0 35.0 2.0 36.0 92-93 27.733418353386504 34.0 27.0 35.0 2.0 35.5 94-95 27.613833698587 34.0 27.0 35.0 2.0 35.0 96-97 27.4923319882232 34.0 27.0 35.0 2.0 35.0 98-99 27.400601047855666 34.0 27.0 35.0 2.0 35.0 100-101 27.297371141788865 34.0 26.5 35.0 2.0 35.0 102-103 26.03974248984853 31.5 24.5 34.0 2.0 35.0 104-105 26.970272588967966 33.5 25.5 35.0 2.0 35.0 106-107 27.13051111378655 34.0 26.0 35.0 2.0 35.0 108-109 27.136702395014478 34.0 26.0 35.0 2.0 35.0 110-111 27.059882518254966 34.0 26.0 35.0 2.0 35.0 112-113 26.974482828587284 34.0 25.0 35.0 2.0 35.0 114-115 26.877916100825168 34.0 25.0 35.0 2.0 35.0 116-117 26.751007990678243 34.0 25.0 35.0 2.0 35.0 118-119 26.61903845813839 34.0 25.0 35.0 2.0 35.0 120-121 26.418596912336596 34.0 25.0 35.0 2.0 35.0 122-123 26.127386532116365 34.0 24.5 35.0 2.0 35.0 124-125 24.35055427910881 31.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7152700941219905 1101 2 0.8054074176502475 1101 3 0.651222451149021 1101 4 0.6296972656249409 1101 5 0.7344596608303107 1101 6 0.7937923173460035 1101 7 0.903731883453041 1101 8 0.914613224645727 1101 9 1.1590599352906565 1101 10-11 1.1803855738841875 1101 12-13 1.0675004168720221 1101 14-15 1.2117378522971975 1101 16-17 1.212037828708958 1101 18-19 1.3010539395013438 1101 20-21 1.2130118420120723 1101 22-23 1.2352331768036109 1101 24-25 1.249634888348396 1101 26-27 1.257355055841181 1101 28-29 1.3628156538088163 1101 30-31 1.3765992353658234 1101 32-33 1.3252988253638591 1101 34-35 1.3951259212603802 1101 36-37 1.411971484781084 1101 38-39 1.2932062671205529 1101 40-41 1.1503669426533065 1101 42-43 1.2389752432412564 1101 44-45 1.2207003356029347 1101 46-47 1.2457248413594328 1101 48-49 1.3895123309350552 1101 50-51 1.3227901366355042 1101 52-53 1.352959149006871 1101 54-55 1.4246074430794096 1101 56-57 1.5147615936425893 1101 58-59 1.4681542431795727 1101 60-61 1.452282033427128 1101 62-63 1.4527335408116286 1101 64-65 1.4420956050677063 1101 66-67 1.4705155711514202 1101 68-69 1.5374753828415173 1101 70-71 1.5164418523982448 1101 72-73 1.3743166153539157 1101 74-75 1.4374640165712336 1101 76-77 1.391321521903027 1101 78-79 1.3159930022184945 1101 80-81 1.14108646542876 1101 82-83 1.300563176582596 1101 84-85 1.26898445164559 1101 86-87 1.2659072814061112 1101 88-89 1.2647293786313334 1101 90-91 1.2274802946600474 1101 92-93 1.2227976676356676 1101 94-95 1.2583045191395854 1101 96-97 1.3235208421317282 1101 98-99 1.2400579446091484 1101 100-101 1.2475705906561707 1101 102-103 1.1391225821913196 1101 104-105 1.2277692505463875 1101 106-107 1.2053133068363202 1101 108-109 1.2911808655894923 1101 110-111 1.1443222173452305 1101 112-113 1.2318088421215911 1101 114-115 1.2890826794575325 1101 116-117 1.369189075279106 1101 118-119 1.2031827414814948 1101 120-121 1.156983371764884 1101 122-123 1.122505350862614 1101 124-125 1.3695082865131702 1102 1 0.34128165481563144 1102 2 -0.44150973071198507 1102 3 0.19668075007552943 1102 4 0.18543632425417655 1102 5 0.5577186754188048 1102 6 0.45838081170328593 1102 7 0.4335970086938872 1102 8 0.5111174910921861 1102 9 0.7139594673578173 1102 10-11 0.8368609385965513 1102 12-13 0.8793363651241606 1102 14-15 1.1448096937505454 1102 16-17 1.0740664551884578 1102 18-19 1.0941175233163776 1102 20-21 1.011332788887934 1102 22-23 1.0486174322398938 1102 24-25 1.0953615605201605 1102 26-27 1.142924832884944 1102 28-29 0.7301773383670351 1102 30-31 0.9368799950685442 1102 32-33 1.0093753461455819 1102 34-35 0.9680546303162529 1102 36-37 0.6792093047645764 1102 38-39 0.24981925913817093 1102 40-41 -0.22760580714025025 1102 42-43 0.25261410919225114 1102 44-45 -0.05178796607612668 1102 46-47 0.1298495316979853 1102 48-49 -0.1740783654591027 1102 50-51 -0.21275211494720736 1102 52-53 -0.3412673855375772 1102 54-55 -0.3097305695823067 1102 56-57 -0.35067330864202617 1102 58-59 -0.3415966513979143 1102 60-61 -0.43901303951533777 1102 62-63 -0.31595624724231897 1102 64-65 -0.339021928650979 1102 66-67 -0.3699055684026682 1102 68-69 -0.07876463917885701 1102 70-71 -0.6002316956336813 1102 72-73 -0.32932362687014205 1102 74-75 -0.2996609490219413 1102 76-77 -0.6722416490300844 1102 78-79 -0.5121586630719008 1102 80-81 -0.5160151035225198 1102 82-83 -0.94465427455971 1102 84-85 -0.8321578549880471 1102 86-87 -0.5228141609280463 1102 88-89 -0.6029144419026622 1102 90-91 -0.5640349819708348 1102 92-93 -0.4596961391355947 1102 94-95 -0.2695581288097664 1102 96-97 -0.5255226480967323 1102 98-99 -0.6599558181213752 1102 100-101 -0.48817122051916684 1102 102-103 -0.4822302942193595 1102 104-105 -0.31274822812138225 1102 106-107 -0.2819148792357957 1102 108-109 -0.21798511294064937 1102 110-111 -0.20132875980863574 1102 112-113 -0.06907197263205589 1102 114-115 -0.15707751872578157 1102 116-117 -0.24058246339416556 1102 118-119 -0.02653787508883454 1102 120-121 0.2773590943082347 1102 122-123 0.2180462261722731 1102 124-125 0.3631017354534407 1103 1 0.4182977181461638 1103 2 0.030744685211633538 1103 3 0.41468847441283785 1103 4 0.484514017729623 1103 5 0.48203904505125195 1103 6 0.5355235974013226 1103 7 0.7183569299017805 1103 8 0.7393995681451955 1103 9 0.9582442966496387 1103 10-11 1.0002933885569405 1103 12-13 0.8931787066889356 1103 14-15 1.035132250977263 1103 16-17 0.9782144146437446 1103 18-19 1.0252549271453049 1103 20-21 1.0853594626960046 1103 22-23 1.147597079224127 1103 24-25 0.9487216391832121 1103 26-27 1.0239520906968096 1103 28-29 1.0780340686879626 1103 30-31 1.0623101332128684 1103 32-33 1.2148958758144275 1103 34-35 1.0763157832558718 1103 36-37 1.181593516335262 1103 38-39 1.2429586263168346 1103 40-41 1.0048777496041055 1103 42-43 1.1277693461090408 1103 44-45 1.0815372740188067 1103 46-47 1.2139717675613042 1103 48-49 1.1230312594717233 1103 50-51 1.0707097287946752 1103 52-53 1.018041546009755 1103 54-55 1.001096650801685 1103 56-57 1.1993765817362743 1103 58-59 1.2529796554718793 1103 60-61 1.1882186776977655 1103 62-63 1.2087533238052899 1103 64-65 1.0704282850266509 1103 66-67 1.0062521545633594 1103 68-69 1.1595297148597261 1103 70-71 1.2197606332669224 1103 72-73 1.0900136337004085 1103 74-75 1.146345199150467 1103 76-77 1.1736261612299934 1103 78-79 1.0722452497534363 1103 80-81 1.1085450787124742 1103 82-83 1.1957786706746099 1103 84-85 1.1096722224973163 1103 86-87 1.09110815850266 1103 88-89 1.0584971855803644 1103 90-91 1.1337643965975168 1103 92-93 0.9938174825106216 1103 94-95 1.0149988026859447 1103 96-97 1.0713319994924753 1103 98-99 1.161687894400341 1103 100-101 1.1460719702709952 1103 102-103 0.859184648533752 1103 104-105 0.8962575296396587 1103 106-107 1.0203180479298943 1103 108-109 1.1805295492647865 1103 110-111 0.9801475920713862 1103 112-113 0.9757854868869593 1103 114-115 0.9769815172807945 1103 116-117 1.0254552968061397 1103 118-119 0.9808052868237915 1103 120-121 0.9842327946175011 1103 122-123 0.9786913083603217 1103 124-125 1.1044047172605929 1104 1 0.2813856726382369 1104 2 0.3422949564056239 1104 3 0.16134555785255245 1104 4 -0.03203797522810703 1104 5 0.13882920935485288 1104 6 0.2969693239547482 1104 7 0.2780534176790823 1104 8 0.32417419091045474 1104 9 0.3316719436546052 1104 10-11 0.4409459038528851 1104 12-13 0.33354057667759207 1104 14-15 0.3775711237903465 1104 16-17 0.3824399056970975 1104 18-19 0.3151771851479239 1104 20-21 0.22618090676216696 1104 22-23 0.2933703188607879 1104 24-25 0.49067187719980154 1104 26-27 0.37572664324979144 1104 28-29 0.5800373295567809 1104 30-31 0.4568491118170286 1104 32-33 0.48981161154576114 1104 34-35 0.6081022025232272 1104 36-37 0.6914767527753938 1104 38-39 0.7552824447550393 1104 40-41 0.7312467995419638 1104 42-43 0.7238843960622958 1104 44-45 0.6099677215104222 1104 46-47 0.5845098475092385 1104 48-49 0.6368351772256204 1104 50-51 0.5520538068689049 1104 52-53 0.6123542520010474 1104 54-55 0.3192225576626839 1104 56-57 0.4621895419326165 1104 58-59 0.3966501477804236 1104 60-61 0.43711618857705403 1104 62-63 0.5491665989429819 1104 64-65 0.5000532790136241 1104 66-67 0.4533987342531951 1104 68-69 0.22529849780794464 1104 70-71 -0.1928548062844584 1104 72-73 -0.18020721785336846 1104 74-75 0.2883310159644097 1104 76-77 0.4875032720486807 1104 78-79 0.499111000133297 1104 80-81 0.4175665642046411 1104 82-83 0.43956017614945253 1104 84-85 0.402045582905199 1104 86-87 0.4367051355780056 1104 88-89 0.4354206571653165 1104 90-91 0.4615472367918443 1104 92-93 0.4385485995804146 1104 94-95 0.42281914736955173 1104 96-97 0.4380720218582539 1104 98-99 0.4471128307995258 1104 100-101 0.5056899437920457 1104 102-103 0.3719906141840106 1104 104-105 0.4127874526127435 1104 106-107 0.4823494409774156 1104 108-109 0.5107164168539491 1104 110-111 0.4616707838897405 1104 112-113 0.4450253790817591 1104 114-115 0.3814521795625332 1104 116-117 0.4290019547739021 1104 118-119 0.45105925478976516 1104 120-121 0.4415505878355397 1104 122-123 0.4627129444162854 1104 124-125 0.7768027442115901 1105 1 0.20869413329263864 1105 2 -0.04652130255537301 1105 3 0.18082286473633147 1105 4 0.2551986913133675 1105 5 -0.04341684655654632 1105 6 0.31774102335732124 1105 7 0.5975715426743307 1105 8 0.5033860980270148 1105 9 0.7141545082694165 1105 10-11 0.6807433517838248 1105 12-13 0.6732960699426016 1105 14-15 0.7770995966999692 1105 16-17 0.7459967310490896 1105 18-19 0.7119618951635829 1105 20-21 0.6702920078960233 1105 22-23 0.8461122038054967 1105 24-25 0.6990331320131276 1105 26-27 0.7026230013002674 1105 28-29 0.643678207250808 1105 30-31 0.7819925039684321 1105 32-33 0.8906540467860538 1105 34-35 0.8753563599604064 1105 36-37 0.8969823580587644 1105 38-39 0.9235388701076062 1105 40-41 0.7472412820593064 1105 42-43 0.6761380819422769 1105 44-45 0.8027533254148693 1105 46-47 0.7775402998676952 1105 48-49 0.7565379208153971 1105 50-51 0.3099832123854398 1105 52-53 0.5440528988765081 1105 54-55 0.6227460119693262 1105 56-57 0.492341654316931 1105 58-59 0.24954921594877888 1105 60-61 0.3858143783468684 1105 62-63 0.5790949380553059 1105 64-65 0.36632963080026215 1105 66-67 0.5604917448271429 1105 68-69 0.6340930884710794 1105 70-71 0.6066373044093254 1105 72-73 0.5790802949733234 1105 74-75 0.5552247501547285 1105 76-77 0.6091059809321493 1105 78-79 0.6500718515525854 1105 80-81 0.5614425792098672 1105 82-83 0.6005919914700435 1105 84-85 0.42031945926116876 1105 86-87 0.6581132828971441 1105 88-89 0.6346895892083175 1105 90-91 0.43023512380839435 1105 92-93 0.5153865680864129 1105 94-95 0.5470779689703207 1105 96-97 0.5682804426128207 1105 98-99 0.5171304187916093 1105 100-101 0.44146599860679103 1105 102-103 0.12372952368936296 1105 104-105 0.43436486375093253 1105 106-107 0.36644666560927774 1105 108-109 0.2898534769669219 1105 110-111 0.32804803311031705 1105 112-113 0.32777838344303944 1105 114-115 0.24626629005926048 1105 116-117 0.005158254044197008 1105 118-119 0.21288726790665535 1105 120-121 0.379425580242831 1105 122-123 0.24371150761575322 1105 124-125 0.783057670064963 1106 1 -0.030273122565063204 1106 2 -0.48737874149717086 1106 3 0.07422094467254325 1106 4 0.04442784161520308 1106 5 0.3579417344454967 1106 6 0.4091019559401303 1106 7 0.46645624165865485 1106 8 0.6338263391002457 1106 9 0.6586372310111841 1106 10-11 0.5826933724983192 1106 12-13 0.6258533663793671 1106 14-15 0.5856354918461477 1106 16-17 0.5241583970811803 1106 18-19 0.6184349546997439 1106 20-21 0.6117293382687308 1106 22-23 0.5379883498199831 1106 24-25 0.48224844800726885 1106 26-27 0.6841042075201784 1106 28-29 0.7508172939151336 1106 30-31 0.6562741379702359 1106 32-33 0.6684616036308526 1106 34-35 0.7325126928207553 1106 36-37 0.6521430106624493 1106 38-39 0.6236755819421589 1106 40-41 0.7467034294222401 1106 42-43 0.5017242715696355 1106 44-45 0.5177173065915497 1106 46-47 0.5042483116690448 1106 48-49 0.6805804901441519 1106 50-51 0.7607172524126184 1106 52-53 0.59408127410952 1106 54-55 0.6047462810480937 1106 56-57 0.1730938705007503 1106 58-59 0.7269566229327076 1106 60-61 0.7088657011205761 1106 62-63 0.6606989067003894 1106 64-65 0.7571588850747446 1106 66-67 0.5396320038516684 1106 68-69 0.5429568328508196 1106 70-71 0.6031463694291972 1106 72-73 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2216 76-77 -1.9094870016110548 2216 78-79 -1.8276791249805804 2216 80-81 -1.7761995584411494 2216 82-83 -1.657313976646197 2216 84-85 -1.6754669845909476 2216 86-87 -1.8145777434454224 2216 88-89 -1.7880385917562407 2216 90-91 -1.7661450104183913 2216 92-93 -1.8339948629607434 2216 94-95 -1.8004711972381742 2216 96-97 -1.7494059410344427 2216 98-99 -1.7491226061431249 2216 100-101 -1.8702305898283953 2216 102-103 -1.6770479155540166 2216 104-105 -1.6899496693872038 2216 106-107 -1.9027630575398184 2216 108-109 -1.8505485504086572 2216 110-111 -1.8772923071450514 2216 112-113 -1.743797481854802 2216 114-115 -1.863378370710553 2216 116-117 -1.892377917841312 2216 118-119 -1.9412338914220264 2216 120-121 -2.1708591847949954 2216 122-123 -2.145409697421602 2216 124-125 -2.3925139169010166 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80837.0 3 27137.0 4 12741.0 5 9735.0 6 10188.0 7 12136.0 8 13598.0 9 14790.0 10 16172.0 11 17741.0 12 19544.0 13 22323.0 14 26381.0 15 23072.0 16 16138.0 17 13857.0 18 12912.0 19 12200.0 20 11993.0 21 12216.0 22 14024.0 23 17231.0 24 22983.0 25 30150.0 26 37149.0 27 40896.0 28 47563.0 29 55752.0 30 64805.0 31 76144.0 32 92240.0 33 113978.0 34 146740.0 35 198381.0 36 314043.0 37 509612.0 38 207424.0 39 692.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53795070251611 20.633503344030636 14.607000552843774 23.22154540060948 2 15.001997459921357 21.485849185672006 40.21053101282794 23.301622341578696 3 18.522402271841344 26.82227623617249 29.843091064769872 24.812230427216296 4 12.333108592136867 18.346761619822175 38.21823774809321 31.10189203994775 5 12.40857783439233 39.396586344536225 34.72619445527249 13.468641365798954 6 29.624401919918096 39.106249668493355 17.875764359604936 13.39358405198361 7 26.80757913170867 33.51648305387987 21.920925122186347 17.755012692225115 8 25.07994564201529 36.816420557017786 19.992556930222065 18.111076870744863 9 25.998407027936786 16.528979546474453 20.5320796115573 36.940533814031454 10-11 23.914455994047575 28.065725041333362 27.7879311850861 20.23188777953296 12-13 25.019322101537433 25.287516238563544 28.449373989167835 21.243787670731184 14-15 22.289342128332933 26.431999104194816 26.561213108812336 24.717445658659916 16-17 21.234572526621122 29.869474371929737 27.144186481179517 21.75176662026962 18-19 21.054334288723012 28.215989211400437 29.88351149803797 20.846165001838582 20-21 21.967238303716606 27.331463541694074 30.048126963412773 20.653171191176547 22-23 21.6514882227792 27.19611049042777 29.81440258503619 21.337998701756838 24-25 21.412824487122386 27.353907652983477 29.98413398677678 21.24913387311736 26-27 21.206490542273308 27.63466747042119 29.918864011975494 21.239977975330014 28-29 21.1626474730985 27.354454902046626 30.219135363318657 21.263762261536222 30-31 21.386367099723092 27.29573086796227 30.083901700597508 21.234000331717123 32-33 21.24824985539996 27.44771877123221 29.965295990179825 21.338735383188006 34-35 21.453847118817876 27.39764548195383 29.902551780285396 21.2459556189429 36-37 21.482388262265324 27.245089281579848 29.731241775478022 21.541280680676806 38-39 21.367402814880794 27.556410854390496 29.890870117591195 21.185316213137515 40-41 21.42600056071981 27.284596454331222 29.99956220074948 21.289840784199487 42-43 21.40829915833094 27.282049641383477 29.65073301907205 21.65891818121353 44-45 21.58371353111195 27.430648810070057 29.380602462284017 21.60503519653398 46-47 21.56209719311746 27.61515593651574 29.030447254030488 21.79229961633631 48-49 21.638270053099998 27.768596154607124 28.762589885658624 21.830543906634258 50-51 21.75021269054273 27.729542663765404 28.60958267189837 21.910661973793495 52-53 21.761506332513584 27.965647913423513 28.430241319998416 21.842604434064487 54-55 21.780702145805673 27.837318008114444 28.54933113535658 21.832648710723305 56-57 21.732630604892915 27.99231090807822 28.383585657974336 21.89147282905453 58-59 21.70733848074415 27.719024028009283 28.578569929637926 21.995067561608646 60-61 21.96363066918457 27.938117075938806 28.454446567022433 21.643805687854186 62-63 21.722251736252893 27.953397953625274 28.663895621923302 21.660454688198534 64-65 21.66870166698835 27.649034744727608 28.68988426282087 21.99237932546317 66-67 21.68569128922618 27.99589394818309 28.33194696904002 21.986467793550712 68-69 21.624020529417162 27.831045691929084 28.624135872681244 21.92079790597251 70-71 21.728439860274683 27.62199654980514 28.620978666547675 22.028584923372502 72-73 21.767568168290033 27.82959337710765 28.27871647362807 22.124121980974255 74-75 21.61341734680647 27.983592451584766 28.238255485600455 22.164734716008304 76-77 21.63454454986239 27.7761313532459 28.499762999059573 22.089561097832135 78-79 21.55887684286122 27.783224543025987 28.406478081833097 22.2514205322797 80-81 21.47099081062229 28.00395451174893 28.236072648683052 22.288982028945732 82-83 21.72820833182489 27.920268337263703 28.21656160887857 22.134961722032838 84-85 21.60838183503556 27.812270839454804 28.159479321983667 22.419868003525966 86-87 21.420401781842948 27.864154260249762 28.457919493769356 22.257524464137926 88-89 21.63897622763145 27.654832331364194 28.38148271787409 22.324708723130268 90-91 21.557803392775806 27.86124963060688 28.391365588473754 22.18958138814355 92-93 21.589551703169292 27.788581494555704 28.287704088688187 22.334162713586814 94-95 21.45222641966931 27.634835854748314 28.34590182015038 22.567035905431997 96-97 21.585021300530084 27.62848625411686 28.34361908194063 22.44287336341243 98-99 21.394114393147827 27.588592679532308 28.27966988602446 22.7376230412954 100-101 21.252307972091327 27.64462239345441 28.38268934632505 22.720380288129213 102-103 21.483945817291218 27.48966751672688 28.354741997324375 22.67164466865753 104-105 21.37039163668724 27.508189792710475 28.365602786423842 22.75581578417844 106-107 21.21671358348057 27.526698803316048 28.4647825008914 22.79180511231199 108-109 21.22468051698209 27.517519866723777 28.162368831212568 23.095430785081568 110-111 21.134129764812908 27.642534475711138 28.169599577017678 23.053736182458273 112-113 21.0775712918193 27.538288491183817 28.41456052953503 22.96957968746185 114-115 21.102029115334002 27.58099496615054 28.167140808867792 23.149835109647665 116-117 20.845895505738117 27.541845610094303 28.327442688289462 23.28481619587812 118-119 20.7806886750595 27.34378354531517 28.35897265354335 23.516555126081975 120-121 20.761711358442227 27.456321698274845 28.330792808061318 23.451174135221613 122-123 20.776492870564663 27.505384885946505 28.263341919261126 23.454780324227702 124-125 20.62938315142399 27.37146793571343 28.26323451031943 23.73591440254315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 788.0 1 556.5 2 508.5 3 715.0 4 539.0 5 377.0 6 705.0 7 1607.5 8 2825.5 9 3339.5 10 2567.5 11 1641.5 12 1333.0 13 1335.5 14 1509.0 15 1700.0 16 1913.0 17 2188.5 18 2511.0 19 2840.5 20 3368.5 21 4109.0 22 4990.5 23 6227.0 24 7798.5 25 10084.5 26 13749.0 27 17991.0 28 21928.5 29 26455.0 30 32054.0 31 38004.5 32 44701.0 33 52321.5 34 60058.5 35 66836.0 36 74974.5 37 82031.5 38 84703.5 39 86496.0 40 85819.5 41 84605.0 42 83972.5 43 84705.5 44 87436.5 45 88513.0 46 87324.0 47 85495.5 48 85499.0 49 84995.0 50 83023.0 51 78966.5 52 73742.0 53 70044.5 54 66278.0 55 60715.5 56 53971.0 57 48339.0 58 43792.0 59 38433.5 60 32119.0 61 25546.0 62 19540.0 63 14565.0 64 10516.0 65 7478.5 66 5336.0 67 3958.5 68 2952.0 69 2133.0 70 1522.0 71 1106.5 72 853.5 73 696.5 74 523.0 75 324.5 76 206.5 77 133.5 78 96.0 79 67.0 80 43.5 81 39.0 82 33.5 83 21.5 84 14.5 85 6.0 86 7.5 87 10.0 88 5.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09825225487662059 2 4.2096081780899996E-5 3 0.0 4 0.10178832574621619 5 0.0 6 0.0 7 0.0020206119254832 8 0.006482796594258599 9 0.0035360708695956 10-11 6.5248926760395E-4 12-13 0.0 14-15 6.314412267135E-5 16-17 3.1572061335675E-4 18-19 0.00199956388459275 20-21 0.0012839304943174498 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.2096081780899996E-5 52-53 0.0 54-55 0.0 56-57 0.008629696765084498 58-59 8.208735947275499E-4 60-61 0.0 62-63 0.0 64-65 2.7362453157584994E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0010944981263033998 76-77 0.0 78-79 0.0 80-81 1.4733628623315E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012207863716461 90-91 0.0 92-93 0.0020837560481545498 94-95 0.0 96-97 0.0094716184007025 98-99 0.0040412238509664 100-101 0.0023573805797304 102-103 0.0 104-105 0.0 106-107 0.0015575550258932998 108-109 2.1048040890449998E-5 110-111 2.104804089045E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010524020445224999 122-123 0.005030481772817549 124-125 0.0876861383496147 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2375518.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.943503715096085 #Duplication Level Percentage of deduplicated Percentage of total 1 75.92411488943102 41.71536888492835 2 12.800155083876078 14.065707368095026 3 4.786033748952646 7.888843891984649 4 2.225753439114783 4.891627694035639 5 1.2234423998739647 3.3610106021340624 6 0.7115642934826589 2.345750124149652 7 0.4720301960043035 1.815449497945999 8 0.33825501856888135 1.4867932695511383 9 0.23930663118017312 1.1833510301365475 >10 1.1358935584888092 11.275889138640254 >50 0.09168101437026425 3.4804133852339922 >100 0.049157693182907115 4.786576188652696 >500 0.0020742618760394324 0.6927096173623972 >1k 3.8412256963623893E-4 0.40584991596668607 >5k 1.5364902785449557E-4 0.6046593911828162 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7769 0.3270444593558121 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6556 0.2759819121555804 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4051 0.17053122729442588 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.2096081780899996E-5 0.0 4 0.0 0.0 0.0 4.2096081780899996E-5 0.0 5 8.419216356179999E-5 0.0 0.0 4.2096081780899996E-5 0.0 6 1.6838432712359998E-4 0.0 0.0 4.2096081780899996E-5 0.0 7 2.525764906854E-4 0.0 0.0 4.2096081780899996E-5 0.0 8 2.525764906854E-4 0.0 0.0 4.2096081780899996E-5 0.0 9 2.525764906854E-4 0.0 0.0 8.419216356179999E-5 0.0 10-11 2.525764906854E-4 0.0 0.0 1.4733628623315E-4 0.0 12-13 2.525764906854E-4 0.0 0.0 1.6838432712359998E-4 0.0 14-15 3.3676865424719997E-4 0.0 0.0 2.104804089045E-4 2.1048040890449998E-5 16-17 3.3676865424719997E-4 0.0 0.0 2.946725724663E-4 4.2096081780899996E-5 18-19 3.788647360281E-4 0.0 0.0 3.3676865424719997E-4 4.2096081780899996E-5 20-21 4.8410494048034997E-4 0.0 0.0 3.9991277691855E-4 4.2096081780899996E-5 22-23 5.262010222612499E-4 0.0 0.0 6.735373084944E-4 4.2096081780899996E-5 24-25 7.366814311657499E-4 2.1048040890449998E-5 0.0 0.0010734500854129498 4.2096081780899996E-5 26-27 7.577294720562E-4 4.2096081780899996E-5 0.0 0.0015996511076742 4.2096081780899996E-5 28-29 8.208735947275499E-4 8.419216356179999E-5 0.0 0.00216794821171635 4.2096081780899996E-5 30-31 8.840177173989E-4 1.4733628623315E-4 0.0 0.00431484838254225 4.2096081780899996E-5 32-33 9.261137991797999E-4 1.6838432712359998E-4 0.0 0.008608648724194048 4.2096081780899996E-5 34-35 0.0010944981263034 1.6838432712359998E-4 0.0 0.014017995233039699 4.2096081780899996E-5 36-37 0.0012628824534269998 1.6838432712359998E-4 0.0 0.023237037143056798 4.2096081780899996E-5 38-39 0.0013470746169887999 2.525764906854E-4 0.0 0.03809695401171449 4.2096081780899996E-5 40-41 0.00136812265787925 2.525764906854E-4 0.0 0.0569980947313386 4.2096081780899996E-5 42-43 0.00149441090322195 2.7362453157585E-4 0.0 0.07592028349185315 4.2096081780899996E-5 44-45 0.0016417471894550999 2.946725724663E-4 0.0 0.09747347736367395 4.2096081780899996E-5 46-47 0.0018732756392500498 2.946725724663E-4 0.0 0.12319418333180385 4.2096081780899996E-5 48-49 0.0019785158437023 2.946725724663E-4 0.0 0.153650698500285 4.2096081780899996E-5 50-51 0.0019785158437023 3.3676865424719997E-4 0.0 0.18684345898452465 4.2096081780899996E-5 52-53 0.0019785158437023 3.3676865424719997E-4 0.0 0.22256198437561828 4.2096081780899996E-5 54-55 0.0020837560481545498 3.3676865424719997E-4 0.0 0.2621743973314452 4.2096081780899996E-5 56-57 0.0021469001708259 3.3676865424719997E-4 0.0 0.30694358030543234 4.2096081780899996E-5 58-59 0.0021469001708259 3.3676865424719997E-4 0.0 0.3587849050186107 4.2096081780899996E-5 60-61 0.0021469001708259 3.3676865424719997E-4 0.0 0.41990841576447746 4.2096081780899996E-5 62-63 0.0021469001708259 3.3676865424719997E-4 0.0 0.4788639782986279 4.2096081780899996E-5 64-65 0.0021889962526068 3.3676865424719997E-4 0.0 0.5428710706464863 4.2096081780899996E-5 66-67 0.0023152844979494997 3.3676865424719997E-4 0.0 0.6148764185327158 4.2096081780899996E-5 68-69 0.0023573805797304 3.3676865424719997E-4 0.0 0.7035518148041816 4.2096081780899996E-5 70-71 0.0023994766615113 3.3676865424719997E-4 0.0 0.8064556867175917 4.2096081780899996E-5 72-73 0.0024415727432922 3.3676865424719997E-4 0.0 0.907780955564218 4.2096081780899996E-5 74-75 0.0024415727432922 3.3676865424719997E-4 0.0 1.012221334462631 4.2096081780899996E-5 76-77 0.0024415727432922 3.3676865424719997E-4 0.0 1.1241548159180441 4.2096081780899996E-5 78-79 0.0024415727432922 3.3676865424719997E-4 0.0 1.2594095266800758 4.2096081780899996E-5 80-81 0.0030309178882248 3.788647360281E-4 0.0 1.4159648548232426 4.2096081780899996E-5 82-83 0.0030940620108961497 3.788647360281E-4 0.0 1.5804973904638904 4.2096081780899996E-5 84-85 0.0031993022153484 3.788647360281E-4 0.0 1.749008005832833 4.2096081780899996E-5 86-87 0.0033255904606910996 3.788647360281E-4 0.0 1.9255800208628182 4.2096081780899996E-5 88-89 0.003367686542472 3.788647360281E-4 0.0 2.1250312563407223 4.2096081780899996E-5 90-91 0.0034518787060338 3.788647360281E-4 0.0 2.3567491385036865 4.2096081780899996E-5 92-93 0.0035360708695955996 3.788647360281E-4 0.0 2.6098097341295667 4.2096081780899996E-5 94-95 0.0035360708695955996 4.20960817809E-4 0.0 2.868069195855388 4.2096081780899996E-5 96-97 0.0035360708695955996 4.4200885869945E-4 0.0 3.126960098807923 4.2096081780899996E-5 98-99 0.0037465512785000996 4.6305689958989996E-4 0.0 3.409256423230638 4.2096081780899996E-5 100-101 0.0039149356056237 4.6305689958989996E-4 0.0 3.7439834175114646 4.2096081780899996E-5 102-103 0.00402017581007595 4.8410494048034997E-4 0.0 4.10672535421748 4.2096081780899996E-5 104-105 0.00444113662788495 5.472490631517E-4 0.0 4.474097859919395 4.2096081780899996E-5 106-107 0.0045674248732276495 5.472490631517E-4 0.0 4.835324337681297 4.2096081780899996E-5 108-109 0.0045884729141180995 5.472490631517E-4 0.0 5.219135363318653 4.2096081780899996E-5 110-111 0.0045884729141180995 5.682971040421501E-4 0.0 5.651377931044934 4.2096081780899996E-5 112-113 0.0046095209550085494 6.524892676039499E-4 0.0 6.126958414964652 4.2096081780899996E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5670 0.0 54.681374 1 GTATCAA 9510 0.0 46.39491 1 ATCAACG 11750 0.0 37.35784 3 TCAACGC 11850 0.0 37.042587 4 CAACGCA 11980 0.0 36.453835 5 AACGCAG 12305 0.0 35.73278 6 TATCAAC 12475 0.0 34.94803 2 ACGCAGA 14250 0.0 30.563295 7 CGCAGAG 14535 0.0 30.004953 8 GTACATG 11575 0.0 29.876253 1 TACATGG 12240 0.0 26.908895 2 GCAGAGT 15755 0.0 26.661287 9 GAGTACT 8940 0.0 26.387638 12-13 ACATGGG 11785 0.0 26.330572 3 GTACTTT 9815 0.0 24.277676 14-15 CATGGGG 4440 0.0 24.145805 4 CAGAGTA 15470 0.0 23.38244 10-11 AGAGTAC 14880 0.0 23.130072 10-11 AGTACTT 9860 0.0 22.77901 12-13 CATGGGA 7660 0.0 21.226873 4 >>END_MODULE