FastQCFastQC Report
Thu 26 May 2016
SRR1364556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615750
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.10402370257096434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.051.838011
GTATCAA64650.049.975961
ATCAACG76950.041.3682143
CAACGCA77500.040.9210785
TCAACGC77700.040.8157464
TATCAAC80600.039.4210172
AACGCAG81400.039.0261236
ACGCAGA91950.034.160237
CGCAGAG92300.033.2617158
GTACATG106400.031.316721
TACATGG106750.030.042942
ACATGGG103450.029.9084843
TAAGGTG16600.027.599695
GCAGAGT108300.027.1945389
AGAGTAC91200.025.54612210-11
GTAAGGT16050.024.0968284
CATGGGG59300.023.5795084
CAGAGTA100650.022.76328510-11
CATGGGA49050.022.6842384
AGGTAAG15350.022.4823232
Produced by FastQC (version 0.11.5)