##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364556_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2615750 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.257952403708305 31.0 28.0 33.0 16.0 34.0 2 28.18844996654879 31.0 28.0 34.0 16.0 34.0 3 28.404755423874605 31.0 28.0 34.0 16.0 34.0 4 31.759295039663577 35.0 32.0 37.0 18.0 37.0 5 31.830086590843926 35.0 32.0 37.0 19.0 37.0 6 31.830083532447674 35.0 32.0 37.0 18.0 37.0 7 31.661547930803785 35.0 32.0 37.0 17.0 37.0 8 31.560584535983942 35.0 32.0 37.0 17.0 37.0 9 32.672566950205486 37.0 32.0 39.0 15.0 39.0 10-11 32.579591895249926 37.0 32.0 39.0 14.0 39.0 12-13 32.49756876612826 37.0 32.0 39.0 11.5 39.0 14-15 33.20590882156169 38.0 32.0 40.5 10.5 41.0 16-17 33.30068813915703 38.0 32.0 40.5 10.0 41.0 18-19 33.09180942368346 38.0 32.0 40.0 10.0 41.0 20-21 32.79811163146326 38.0 31.0 40.0 10.0 41.0 22-23 32.69345866386314 38.0 31.0 40.0 9.5 41.0 24-25 32.501871547357354 38.0 31.0 40.0 8.0 41.0 26-27 32.391483322182935 38.0 30.5 40.0 8.0 41.0 28-29 32.456335850138586 38.0 30.5 40.0 8.0 41.0 30-31 32.391919334798814 38.0 30.5 40.0 8.0 41.0 32-33 32.36887374557966 38.0 30.5 40.0 4.5 41.0 34-35 31.90151906718914 38.0 30.0 40.0 2.0 41.0 36-37 31.76324247347797 37.5 30.0 40.0 2.0 41.0 38-39 31.562365669502057 37.0 30.0 40.0 2.0 41.0 40-41 31.757676192296664 37.0 30.0 40.0 2.0 41.0 42-43 31.651068527191054 37.0 30.0 40.0 2.0 41.0 44-45 31.49138717385071 37.0 30.0 40.0 2.0 41.0 46-47 31.456783905189717 37.0 30.0 40.0 2.0 41.0 48-49 31.307586543056484 37.0 30.0 40.0 2.0 41.0 50-51 31.13602618751792 37.0 29.5 40.0 2.0 41.0 52-53 31.05753263882252 36.5 29.5 40.0 2.0 41.0 54-55 31.07817566663481 37.0 29.5 40.0 2.0 41.0 56-57 30.9337996750454 36.0 29.0 40.0 2.0 41.0 58-59 30.719461339959857 36.0 29.0 40.0 2.0 41.0 60-61 30.499192965688618 36.0 29.0 39.5 2.0 41.0 62-63 30.197556723693012 35.0 28.0 39.0 2.0 41.0 64-65 29.908144891522507 35.0 28.0 39.0 2.0 41.0 66-67 29.653369014622957 35.0 28.0 39.0 2.0 40.0 68-69 29.609843639491544 35.0 28.0 38.5 2.0 40.0 70-71 29.378742807990058 35.0 28.0 37.5 2.0 40.0 72-73 29.100891140208354 35.0 28.0 37.0 2.0 40.0 74-75 28.81285176335659 34.0 28.0 36.5 2.0 39.0 76-77 28.522041479499187 34.0 28.0 36.0 2.0 39.0 78-79 28.222990920386124 34.0 27.5 36.0 2.0 38.5 80-81 27.943516773391956 34.0 27.0 35.0 2.0 37.0 82-83 27.70716754276976 34.0 26.5 35.0 2.0 37.0 84-85 27.470134378285387 34.0 26.0 35.0 2.0 36.5 86-87 27.290144509222976 34.0 26.0 35.0 2.0 36.0 88-89 27.09738679155118 34.0 26.0 35.0 2.0 36.0 90-91 26.962499856637677 34.0 26.0 35.0 2.0 35.5 92-93 26.842590843926217 34.0 26.0 35.0 2.0 35.0 94-95 26.716350759820322 33.5 25.0 35.0 2.0 35.0 96-97 26.60809156073784 33.0 25.0 35.0 2.0 35.0 98-99 26.519597438593138 33.0 25.0 35.0 2.0 35.0 100-101 26.423617509318554 33.0 25.0 35.0 2.0 35.0 102-103 25.236306986523942 31.5 21.5 34.0 2.0 35.0 104-105 26.127059160852525 33.0 24.0 35.0 2.0 35.0 106-107 26.278999330975818 33.0 25.0 35.0 2.0 35.0 108-109 26.237449106374847 33.0 24.0 35.0 2.0 35.0 110-111 26.147725508936254 33.0 24.0 35.0 2.0 35.0 112-113 26.048330497945138 33.0 24.0 35.0 2.0 35.0 114-115 25.921309758195548 33.0 23.5 35.0 2.0 35.0 116-117 25.809424256905288 33.0 23.0 35.0 2.0 35.0 118-119 25.656333365191628 33.0 21.5 35.0 2.0 35.0 120-121 25.475903660518014 33.0 19.0 35.0 2.0 35.0 122-123 25.232778935295805 33.0 14.0 35.0 2.0 35.0 124-125 23.793383924304692 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0195078898362375 1101 2 1.0024785392327757 1101 3 1.0304068937795137 1101 4 1.109523366233116 1101 5 1.0667968129340757 1101 6 1.148069401283184 1101 7 1.156990359795934 1101 8 1.0077930961360266 1101 9 1.4122986642027016 1101 10-11 1.513235429193628 1101 12-13 1.1630510096796982 1101 14-15 1.5335280911031148 1101 16-17 1.2837498590220804 1101 18-19 1.719198304729467 1101 20-21 2.0721138809147135 1101 22-23 1.9837569886950277 1101 24-25 2.1672617743755325 1101 26-27 2.246171615774969 1101 28-29 2.101272911741191 1101 30-31 2.1527982253083024 1101 32-33 2.0467798956711505 1101 34-35 2.3112703859103014 1101 36-37 2.2467292664170415 1101 38-39 2.058004822796203 1101 40-41 1.8632207650459698 1101 42-43 1.9404873359216488 1101 44-45 2.0351094843877036 1101 46-47 1.7864141502874737 1101 48-49 1.7987742082388891 1101 50-51 1.7586776428738347 1101 52-53 1.6268241228590234 1101 54-55 1.5170540472045317 1101 56-57 1.4528948077785522 1101 58-59 1.515752136532818 1101 60-61 1.4702613410472694 1101 62-63 1.491304498203089 1101 64-65 1.5644735998610777 1101 66-67 1.4932755362522592 1101 68-69 1.3160998564093198 1101 70-71 1.2882878211176383 1101 72-73 1.227444231381238 1101 74-75 1.295164185000786 1101 76-77 1.4861815334650714 1101 78-79 1.5002585618428483 1101 80-81 1.5186466815717594 1101 82-83 1.2893388788894384 1101 84-85 1.3933472515741308 1101 86-87 1.4180369369889334 1101 88-89 1.5228950793759601 1101 90-91 1.48509907038326 1101 92-93 1.295187635463801 1101 94-95 1.359367913955296 1101 96-97 1.2693124814127934 1101 98-99 1.355018709972036 1101 100-101 1.4070763263455675 1101 102-103 1.4153503996086485 1101 104-105 1.4969281120111297 1101 106-107 1.3614238203686178 1101 108-109 1.4363496757728669 1101 110-111 1.3007084459701304 1101 112-113 1.2681379029114552 1101 114-115 1.5826859489733067 1101 116-117 1.4993665632443225 1101 118-119 1.4973936096259806 1101 120-121 1.6445997433444468 1101 122-123 1.7204265243455836 1101 124-125 1.859347042567272 1102 1 0.0762409734177929 1102 2 0.26929541829789727 1102 3 0.3495852665294876 1102 4 0.5617822457584118 1102 5 0.5806998850269061 1102 6 -0.31800213105265485 1102 7 -0.8115767577598199 1102 8 -0.0073706995664295505 1102 9 0.3683439488892404 1102 10-11 0.3212796900837489 1102 12-13 0.4792614737182319 1102 14-15 0.7207159719487422 1102 16-17 0.5947520616566422 1102 18-19 0.7558866776696576 1102 20-21 1.0376411386802857 1102 22-23 0.885811506310759 1102 24-25 1.0408562365983869 1102 26-27 0.4911727583932759 1102 28-29 1.0488309005974727 1102 30-31 0.9670481283095285 1102 32-33 0.8113833914838509 1102 34-35 1.101230953201398 1102 36-37 1.3063701637619758 1102 38-39 1.3456271266013466 1102 40-41 1.0406454805704932 1102 42-43 0.9336620155520556 1102 44-45 1.012374995528944 1102 46-47 0.7729790696384207 1102 48-49 0.4877311605827188 1102 50-51 0.8230429151466154 1102 52-53 0.5663401699364599 1102 54-55 0.6056878092433919 1102 56-57 0.4650847667042264 1102 58-59 0.6962903566592153 1102 60-61 0.48168140063018683 1102 62-63 0.36006266883599736 1102 64-65 0.30236197608450865 1102 66-67 0.3020990771599479 1102 68-69 0.24624223160139636 1102 70-71 0.3287998287722651 1102 72-73 0.46870831509646393 1102 74-75 0.10739180342724453 1102 76-77 0.26050002092506475 1102 78-79 0.20832033332596467 1102 80-81 -0.12830963471019885 1102 82-83 0.11230176763698196 1102 84-85 -0.16698501453372572 1102 86-87 0.29160798009651856 1102 88-89 0.49576128202928516 1102 90-91 -0.054666229041369974 1102 92-93 0.26722469069278887 1102 94-95 0.2904084887613543 1102 96-97 0.371492687042398 1102 98-99 0.529054526430798 1102 100-101 0.5375972769101942 1102 102-103 0.34120137664501726 1102 104-105 0.6733093903208989 1102 106-107 0.5149897014993101 1102 108-109 0.056831317401382364 1102 110-111 0.2771601509989807 1102 112-113 -0.0013810990785501076 1102 114-115 0.0373087259813758 1102 116-117 -0.004943726548173544 1102 118-119 0.34175710738777454 1102 120-121 0.6588935140291596 1102 122-123 0.5036364583759863 1102 124-125 0.9511419943050043 1103 1 0.7944644141709496 1103 2 0.739081600451378 1103 3 0.6387016217922898 1103 4 0.6986070298928801 1103 5 0.8599431969351308 1103 6 0.8083095540105028 1103 7 0.9501508531647787 1103 8 0.9849093947007788 1103 9 1.128506979172954 1103 10-11 1.244027907859639 1103 12-13 1.2797627280802928 1103 14-15 1.6396594140751972 1103 16-17 1.4712911994697109 1103 18-19 1.483973352494381 1103 20-21 1.9224311096693327 1103 22-23 1.957388618310695 1103 24-25 1.879254429728448 1103 26-27 1.9186576337050312 1103 28-29 1.7759771104131374 1103 30-31 1.7189897532662997 1103 32-33 1.7098475029957783 1103 34-35 2.0570450378604157 1103 36-37 2.068877612686258 1103 38-39 2.1804841685630016 1103 40-41 1.855126189389285 1103 42-43 1.6813341500765695 1103 44-45 1.3308100715631497 1103 46-47 1.4230684567298972 1103 48-49 1.4940446603632367 1103 50-51 1.4194522270989864 1103 52-53 1.4362371969880066 1103 54-55 1.315107418490598 1103 56-57 1.2473996103565135 1103 58-59 1.2413137395376026 1103 60-61 1.18690999370925 1103 62-63 1.0935015337057692 1103 64-65 1.27960076100862 1103 66-67 1.2729066509073625 1103 68-69 1.288750759546815 1103 70-71 1.2957682943796947 1103 72-73 1.3258201678983639 1103 74-75 1.1984244021270243 1103 76-77 1.2588508352181726 1103 78-79 1.2472815793010916 1103 80-81 1.306261221419497 1103 82-83 1.3485669321754408 1103 84-85 1.2402853808049024 1103 86-87 1.1666536545705597 1103 88-89 1.2104080129631036 1103 90-91 1.2581272830988475 1103 92-93 1.3026202622919705 1103 94-95 1.2890364152996376 1103 96-97 1.3803995160199989 1103 98-99 1.3251943283323264 1103 100-101 1.3703539061462706 1103 102-103 1.1827360880191264 1103 104-105 1.2435144288591182 1103 106-107 1.3532321722615173 1103 108-109 1.5270049662339567 1103 110-111 1.517399301318882 1103 112-113 1.4370362221533988 1103 114-115 1.5136769370379106 1103 116-117 1.4633575753889119 1103 118-119 1.5606316393010538 1103 120-121 1.5649500105946856 1103 122-123 1.5197469795677172 1103 124-125 1.6201927254644382 1104 1 0.5648609789600378 1104 2 0.5953517909566877 1104 3 0.5292375093768769 1104 4 0.6566838967252799 1104 5 0.6614148604333572 1104 6 0.6110574568567877 1104 7 0.7081439667899616 1104 8 0.6834443782824948 1104 9 1.0408984655929672 1104 10-11 0.9908373094894998 1104 12-13 0.9527230716013904 1104 14-15 1.091979909681477 1104 16-17 1.1280825210421384 1104 18-19 1.3384488314164926 1104 20-21 1.6557967921250238 1104 22-23 1.445722993019892 1104 24-25 1.840837631228034 1104 26-27 1.7839445881459426 1104 28-29 1.599799580202152 1104 30-31 1.636539373718037 1104 32-33 1.6384744821638293 1104 34-35 1.9202713975206187 1104 36-37 2.001546016456487 1104 38-39 2.0315962250365978 1104 40-41 1.6378497639229899 1104 42-43 1.6631842983064509 1104 44-45 1.5497117080742342 1104 46-47 1.5499479185823084 1104 48-49 1.426596740234551 1104 50-51 1.273495645825136 1104 52-53 1.3954008613902396 1104 54-55 1.2256244283681639 1104 56-57 1.1503455961849376 1104 58-59 1.0949118039079266 1104 60-61 1.0700975344226116 1104 62-63 1.1160550759346677 1104 64-65 1.0774830013404433 1104 66-67 1.1474463406216735 1104 68-69 1.2380202454616551 1104 70-71 1.1096666738216072 1104 72-73 1.059717616749083 1104 74-75 1.0908513839539076 1104 76-77 1.0551311292849874 1104 78-79 1.104631920296292 1104 80-81 1.0862029929992438 1104 82-83 1.0910191729060905 1104 84-85 1.09129108399949 1104 86-87 1.0893447913541685 1104 88-89 1.0795336560680866 1104 90-91 1.108228983421892 1104 92-93 1.1196361222247013 1104 94-95 1.1221847382413692 1104 96-97 0.9846822296239139 1104 98-99 1.0645357356520933 1104 100-101 1.0693840971833488 1104 102-103 1.099222175680815 1104 104-105 1.2065599879450097 1104 106-107 1.1590576731189906 1104 108-109 1.2070378028088484 1104 110-111 1.2761003289574404 1104 112-113 1.1969968714401666 1104 114-115 1.1826091501167042 1104 116-117 1.2452203530706782 1104 118-119 1.2148538644775329 1104 120-121 1.2749246187493242 1104 122-123 1.3170376297850765 1104 124-125 1.5749859348075717 1105 1 0.40812802508001056 1105 2 0.6729991804584792 1105 3 0.6123003065567403 1105 4 0.7564520085937367 1105 5 0.6154892988658851 1105 6 0.7040820744755969 1105 7 0.8985967342225649 1105 8 0.7067409374373881 1105 9 0.6075651322596372 1105 10-11 0.8197694529322206 1105 12-13 0.7674854448241675 1105 14-15 0.9971458619453202 1105 16-17 0.891254469605137 1105 18-19 0.977751527408806 1105 20-21 1.3325763578617966 1105 22-23 1.537302408849179 1105 24-25 1.5821090340341186 1105 26-27 1.4307368023262512 1105 28-29 1.105781825535118 1105 30-31 1.0115973457509213 1105 32-33 1.0943161892352506 1105 34-35 1.6560828888658001 1105 36-37 1.7676353050986506 1105 38-39 1.878149524172283 1105 40-41 1.3928845471427174 1105 42-43 1.0203154439275046 1105 44-45 1.0737378950369703 1105 46-47 1.1489383854035573 1105 48-49 1.0145069362854215 1105 50-51 0.9548555877359917 1105 52-53 0.909397301087612 1105 54-55 0.7161650322142279 1105 56-57 0.7981742800441225 1105 58-59 0.7462375481751344 1105 60-61 0.7194006988758019 1105 62-63 0.7577867415472959 1105 64-65 0.9119933426141884 1105 66-67 0.6480729070377116 1105 68-69 0.573486246913884 1105 70-71 0.8179303236002582 1105 72-73 0.7700448366680384 1105 74-75 0.7909626434109605 1105 76-77 0.8810815192786663 1105 78-79 0.8120675654840284 1105 80-81 0.6766206258664589 1105 82-83 0.6777769552123196 1105 84-85 0.7782245391326477 1105 86-87 0.754744271362604 1105 88-89 0.9290874470876922 1105 90-91 0.6774183118832333 1105 92-93 0.587992761486408 1105 94-95 0.715354578730441 1105 96-97 0.8423216552908848 1105 98-99 0.7253454702690085 1105 100-101 0.72964690373243 1105 102-103 0.6586780583782144 1105 104-105 0.7717744259090829 1105 106-107 0.8029829067638481 1105 108-109 0.8054930530840707 1105 110-111 0.8942063416527333 1105 112-113 0.9067994151827037 1105 114-115 0.836247919938458 1105 116-117 0.8705090420829258 1105 118-119 0.9657661920253737 1105 120-121 0.8802135419920099 1105 122-123 0.9664614229127579 1105 124-125 1.1199265573920734 1106 1 0.5000001311125786 1106 2 0.7158415657372288 1106 3 0.4662977813711038 1106 4 0.615981431042659 1106 5 0.5298413591878024 1106 6 0.4934889081173246 1106 7 0.3239473528425947 1106 8 0.5531888387709998 1106 9 0.7124609904254342 1106 10-11 0.9839716670735115 1106 12-13 0.8462976615764077 1106 14-15 1.1127673893420678 1106 16-17 1.0431924423679462 1106 18-19 1.0829036881327099 1106 20-21 1.4208295146591752 1106 22-23 1.4604800055838467 1106 24-25 1.706172526938495 1106 26-27 1.8102752306819951 1106 28-29 1.6717010271627402 1106 30-31 1.526886764567216 1106 32-33 1.3622327723387073 1106 34-35 1.7064793884186606 1106 36-37 1.5373513037010937 1106 38-39 1.728232204451242 1106 40-41 1.4469515843885752 1106 42-43 1.4388104105842139 1106 44-45 1.3053520966204388 1106 46-47 1.0133595036243754 1106 48-49 1.1797490551709586 1106 50-51 1.0136077146562101 1106 52-53 1.098470197218223 1106 54-55 0.8868422284761408 1106 56-57 0.9530862832529508 1106 58-59 0.9278543985547323 1106 60-61 1.0322601625828796 1106 62-63 1.0266676756389437 1106 64-65 0.9848680305923097 1106 66-67 0.8644224749686096 1106 68-69 0.8697808334052759 1106 70-71 0.9123559321611694 1106 72-73 0.9669134551190481 1106 74-75 0.9994773246788 1106 76-77 0.8206923732883737 1106 78-79 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2216 102-103 -2.917514340331252 2216 104-105 -3.136647955008346 2216 106-107 -3.163325297498549 2216 108-109 -3.155288782956518 2216 110-111 -3.2037295720506336 2216 112-113 -3.252085768487774 2216 114-115 -3.3280848086752712 2216 116-117 -3.3216727722856305 2216 118-119 -3.407204064858117 2216 120-121 -3.446142270831544 2216 122-123 -3.5062312921384695 2216 124-125 -3.5741487634064377 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 174811.0 3 29847.0 4 18582.0 5 14714.0 6 13532.0 7 14263.0 8 15153.0 9 15752.0 10 16522.0 11 17694.0 12 18711.0 13 20176.0 14 21973.0 15 19683.0 16 15750.0 17 14409.0 18 13389.0 19 12772.0 20 12779.0 21 13582.0 22 16378.0 23 21343.0 24 28658.0 25 36292.0 26 43951.0 27 50910.0 28 58985.0 29 68287.0 30 77570.0 31 89859.0 32 109357.0 33 136554.0 34 175956.0 35 239427.0 36 362256.0 37 454614.0 38 150837.0 39 422.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1934889383221 18.596091038324158 13.337330132834957 23.87308989051879 2 15.133239257954001 19.966753745651626 40.39653116473144 24.50347583166293 3 19.243080226399975 25.00321156407759 28.622797211138927 27.130910998383513 4 12.458225787396833 16.50697425282417 37.48524688315914 33.54955307661986 5 13.690130246728538 37.97787212695538 34.091079414409734 14.24091821190635 6 32.00191609697317 36.690236851300625 17.427918893893647 13.879928157832563 7 28.50101967549315 32.19475304889472 21.03766032074472 18.26656695486741 8 26.120156742807993 35.52086399694161 19.598738411545447 18.76024084870496 9 26.319485781418873 16.708013198776463 19.562395797372556 37.41010522243211 10-11 24.86244862850043 26.75890280034407 27.30329733346077 21.075351237694733 12-13 25.74044143860853 23.867849418070637 27.526783469899 22.864925673421833 14-15 22.687858453587396 25.488533117775248 25.021116713769015 26.80249171486835 16-17 22.66644588162926 28.671486587595794 25.77590101997716 22.886166510797786 18-19 22.68542847284416 27.36336534959985 27.65215213732235 22.29905404023364 20-21 23.22960385771567 26.612694331471065 27.866948954086872 22.29075285672639 22-23 23.011430811752216 26.43201506598537 27.67233622746702 22.884217894795395 24-25 22.81885109417327 26.660568278055933 27.70801909426503 22.81256153350577 26-27 22.498246330485973 26.912102011658245 27.505797290143256 23.08385436771252 28-29 22.653710980882252 27.021294572046045 27.540727527270516 22.784266919801187 30-31 22.679037056308072 26.883442860701027 27.356087270427874 23.081432812563023 32-33 22.574818888509952 26.84263831687514 27.200426225647252 23.382116568967653 34-35 23.012180283101017 26.732657936014085 27.48006832352162 22.775093457363273 36-37 23.07975831208451 26.335043562476514 27.426007641546985 23.159190483891997 38-39 22.688850748131653 26.790065333090958 27.392932694816537 23.128151223960856 40-41 23.050090437349667 26.712401580072427 27.30871448947829 22.92879349309961 42-43 22.88510220490992 26.672474393811314 26.993046403319315 23.449376997959455 44-45 22.88582315120532 26.942360010476868 27.168090666982756 23.003726171335057 46-47 23.284605897299244 27.068462837747425 26.588140254882365 23.058791010070966 48-49 23.04379989243091 27.12909265874423 26.52688930880646 23.300218140018398 50-51 23.063725846274068 27.253595866712647 26.56445339232204 23.11822489469125 52-53 23.355557679441844 26.841173659562266 26.676956895727805 23.12631176526809 54-55 23.179584712968097 27.01025188359697 26.672084833892264 23.13807856954267 56-57 22.99709668194407 27.300135952054344 26.531333966450887 23.1714333995507 58-59 23.05052671418659 26.888094978677202 26.64544900210074 23.41592930503547 60-61 22.851004481140098 27.064159328406284 26.808312646785915 23.2765235436677 62-63 22.930579580929493 27.006083935324703 27.066255984939207 22.997080498806596 64-65 22.772077714257748 27.027656386159304 26.784756314159168 23.415509585423777 66-67 22.770161480705255 27.216467335028355 26.574344644711672 23.439026539554717 68-69 23.023378711106062 26.942875704976792 26.95667984751967 23.07706573639747 70-71 22.960403460604077 26.84808732598391 26.733546905086097 23.457962308325914 72-73 22.942899403363256 26.985706450287633 26.603720524942453 23.46767362140666 74-75 22.835424416596467 27.323449741651483 26.540729530866773 23.300396310885276 76-77 23.046309281125314 26.955353824837857 26.70082214783062 23.297514746206208 78-79 23.07615700103375 27.09180771089349 26.657683826452903 23.174351461619864 80-81 23.12166396275968 27.16941941410952 26.467932848293135 23.240983774837666 82-83 23.190742848732132 27.19593073766849 26.54308633515024 23.07024007844914 84-85 22.89576603268661 27.31132562362611 26.5724935486954 23.220414794991875 86-87 23.093137961448896 27.268367058636418 26.536869459430424 23.101625520484266 88-89 23.001333317528786 27.299533013852052 26.351948413956283 23.347185254662882 90-91 22.883601500115194 27.42212870667165 26.446269930873484 23.24799986233967 92-93 23.131369668135918 27.2057398267867 26.544137353337778 23.118753151739607 94-95 22.86712618184947 26.959590069118477 26.714013938414766 23.459269810617286 96-97 23.10927767722442 26.7796144193453 26.772406696796345 23.338701206633935 98-99 22.758922181511227 27.060382425083958 26.523839634702078 23.65685575870274 100-101 22.784683084556857 26.79055117748974 26.899956623980763 23.52480911397264 102-103 22.668699225843447 26.517041001624776 27.106814489152253 23.707445283379528 104-105 22.591547626557173 26.86976601044074 26.807603274821606 23.73108308818048 106-107 22.60958092508476 26.840070121393506 26.731114734037032 23.819234219484702 108-109 22.6423396731339 26.727936538277742 26.666577463442607 23.96314632514575 110-111 22.611817562489154 26.807599702416223 26.820655311281648 23.75992742381297 112-113 22.587441460384213 26.88794800726369 26.91123004874319 23.61338048360891 114-115 22.55056867055338 27.104769186657744 26.70455892191532 23.640103220873556 116-117 22.395737360221734 27.05866386313677 26.843658606518204 23.701940170123294 118-119 22.299933097581956 27.103220873554427 26.66785816687375 23.92898786198987 120-121 22.176225208109567 27.218756994888082 26.778691919849358 23.826325877152993 122-123 22.144700372742044 27.15840581095288 26.848399120711075 23.848494695593995 124-125 22.225264309478167 27.127041961238753 26.708712476294505 23.93898125298858 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 463.0 1 493.0 2 595.5 3 633.5 4 547.0 5 457.5 6 442.0 7 526.5 8 723.5 9 835.0 10 816.0 11 809.0 12 856.5 13 967.5 14 1077.0 15 1222.0 16 1384.0 17 1568.5 18 1758.5 19 1982.5 20 2354.0 21 2946.0 22 3863.5 23 5172.5 24 6833.0 25 8969.5 26 12087.5 27 16044.5 28 19658.5 29 23637.5 30 28566.0 31 34893.5 32 42503.0 33 47159.5 34 49549.5 35 54006.5 36 60920.0 37 66978.0 38 70169.5 39 72729.0 40 75267.5 41 77934.0 42 79101.0 43 79886.5 44 82940.5 45 86900.0 46 88812.0 47 89902.0 48 92168.5 49 92436.5 50 91625.0 51 91642.5 52 93531.5 53 96088.5 54 95971.0 55 91514.5 56 84911.0 57 79089.0 58 74865.5 59 67664.0 60 56507.5 61 46729.5 62 38087.0 63 28823.5 64 20641.5 65 15329.0 66 11763.5 67 9190.5 68 7192.5 69 5680.0 70 4310.5 71 3069.0 72 2284.0 73 1810.5 74 1433.5 75 1139.0 76 804.0 77 512.0 78 362.0 79 238.5 80 149.0 81 99.0 82 82.5 83 59.5 84 39.5 85 33.5 86 31.5 87 27.5 88 17.0 89 9.0 90 10.0 91 11.5 92 9.0 93 7.5 94 7.0 95 4.5 96 4.5 97 4.5 98 4.5 99 5.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012386504826531586 2 0.004472904520691962 3 0.007722450539998089 4 0.007684220586829781 5 0.015521360986332791 6 6.116792506929179E-4 7 0.008104750071681162 8 0.0 9 0.0020261875179202905 10-11 0.0 12-13 0.0037274204339099687 14-15 0.019994265507024756 16-17 0.03205581573162573 18-19 0.005830067858166874 20-21 0.008123865048265316 22-23 0.0021599923540093664 24-25 0.011660135716333748 26-27 0.01986046067093568 28-29 4.969893911879958E-4 30-31 0.0015865430564847558 32-33 0.008945809041383925 34-35 0.00965306317499761 36-37 0.011889515435343593 38-39 1.9114976584153684E-5 40-41 0.02599636815444901 42-43 0.010283857402274683 44-45 0.017776928223262926 46-47 0.004281754754850425 48-49 0.026244862850043005 50-51 0.004128834942177196 52-53 0.0 54-55 0.018140112778361847 56-57 0.004664054286533499 58-59 1.9114976584153686E-4 60-61 0.004625824333365192 62-63 0.028615119946478064 64-65 0.018235687661282612 66-67 0.0019306126349995223 68-69 0.02267036222880627 70-71 0.003039281276880436 72-73 0.012979069100640351 74-75 1.9114976584153684E-5 76-77 0.006193252413265793 78-79 8.410589697027621E-4 80-81 0.003459810761731817 82-83 0.0016247730096530632 84-85 0.0 86-87 0.006002102647424257 88-89 0.01792984803593616 90-91 0.023606996081429802 92-93 0.005008123865048265 94-95 0.025805218388607477 96-97 0.01909586160756953 98-99 8.410589697027621E-4 100-101 0.009538373315492689 102-103 0.0 104-105 0.001338048360890758 106-107 1.9114976584153684E-5 108-109 0.0 110-111 6.116792506929179E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.9114976584153684E-5 122-123 0.0 124-125 0.022421867533212275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2615750.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.09109951741361 #Duplication Level Percentage of deduplicated Percentage of total 1 76.79653386606459 42.30805489807789 2 11.973181040033365 13.192314164329758 3 4.547581349571566 7.515937700783436 4 2.1914428441531744 4.829159832558657 5 1.1989232169523893 3.3024999129430874 6 0.7615126324010869 2.5171540929225533 7 0.4923654388264861 1.8987467372527476 8 0.3408642187001145 1.5022867675466756 9 0.2583206774308652 1.2808053132974544 >10 1.3084032371068046 12.869303972098978 >50 0.0901915230266605 3.3969121863029685 >100 0.03763948852154778 3.6743237488280753 >500 0.0018384220348706382 0.7217028335792841 >1k 0.0012020451766399408 0.9907978394785315 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2721 0.10402370257096434 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1468985950492211E-4 2 0.0 0.0 0.0 0.0 1.1468985950492211E-4 3 0.0 0.0 0.0 0.0 1.1468985950492211E-4 4 0.0 0.0 0.0 0.0 1.5291981267322947E-4 5 0.0 0.0 0.0 0.0 1.9114976584153686E-4 6 0.0 0.0 0.0 0.0 1.9114976584153686E-4 7 0.0 0.0 0.0 0.0 1.9114976584153686E-4 8 0.0 0.0 0.0 0.0 2.2937971900984422E-4 9 0.0 0.0 0.0 0.0 2.2937971900984422E-4 10-11 0.0 0.0 0.0 0.0 2.2937971900984422E-4 12-13 0.0 0.0 0.0 0.0 5.543343209404568E-4 14-15 3.822995316830737E-5 0.0 0.0 3.822995316830737E-5 7.837140399503011E-4 16-17 7.645990633661473E-5 0.0 0.0 1.1468985950492211E-4 9.939787823759916E-4 18-19 7.645990633661473E-5 0.0 0.0 1.338048360890758E-4 0.001032208735544299 20-21 7.645990633661473E-5 3.822995316830737E-5 0.0 1.9114976584153686E-4 0.0012424734779699896 22-23 7.645990633661473E-5 3.822995316830737E-5 0.0 2.676096721781516E-4 0.0012998184077224505 24-25 7.645990633661473E-5 3.822995316830737E-5 0.0 5.352193443563032E-4 0.001338048360890758 26-27 7.645990633661473E-5 3.822995316830737E-5 0.0 8.410589697027621E-4 0.0014336232438115263 28-29 1.1468985950492211E-4 3.822995316830737E-5 0.0 0.0014145082672273727 0.0014909681735639875 30-31 1.5291981267322947E-4 3.822995316830737E-5 0.0 0.002848131511038899 0.0015291981267322946 32-33 1.5291981267322947E-4 3.822995316830737E-5 0.0 0.006097677530345026 0.001758577845742139 34-35 1.9114976584153686E-4 3.822995316830737E-5 0.0 0.01047500716811622 0.0018350377520787537 36-37 1.9114976584153686E-4 3.822995316830737E-5 0.0 0.01836949249737169 0.0018350377520787537 38-39 3.0583962534645894E-4 3.822995316830737E-5 0.0 0.03283952977157603 0.0018732677052470611 40-41 3.440695785147663E-4 3.822995316830737E-5 0.0 0.049412214470037276 0.0018732677052470611 42-43 3.822995316830737E-4 3.822995316830737E-5 0.0 0.06361464207206347 0.001930612634999522 44-45 4.2052948485138105E-4 3.822995316830737E-5 0.0 0.08236643410111823 0.0020261875179202905 46-47 4.7787441460384213E-4 3.822995316830737E-5 0.0 0.10631749976106279 0.00217910733059352 48-49 4.969893911879958E-4 3.822995316830737E-5 0.0 0.1335945713466501 0.0022746822135142884 50-51 4.969893911879958E-4 3.822995316830737E-5 0.0 0.1657841919143649 0.0023320271432667496 52-53 4.969893911879958E-4 3.822995316830737E-5 0.0 0.19671222402752558 0.002446717002771672 54-55 4.969893911879958E-4 3.822995316830737E-5 0.0 0.2288062697123196 0.0025231769091082863 56-57 5.352193443563032E-4 3.822995316830737E-5 0.0 0.2690815253751314 0.0025614068622765935 58-59 5.352193443563032E-4 3.822995316830737E-5 0.0 0.3146898595049221 0.002599636815444901 60-61 5.352193443563032E-4 3.822995316830737E-5 0.0 0.36465640829589985 0.002599636815444901 62-63 5.352193443563032E-4 3.822995316830737E-5 0.0 0.4175857784574214 0.0027907865812864378 64-65 5.352193443563032E-4 3.822995316830737E-5 0.0 0.4744146038421103 0.0028672464876230526 66-67 5.352193443563032E-4 3.822995316830737E-5 0.0 0.5416228615119947 0.0029054764407913597 68-69 5.352193443563032E-4 3.822995316830737E-5 0.0 0.620032495460193 0.0029437063939596673 70-71 5.352193443563032E-4 3.822995316830737E-5 0.0 0.7067762591990825 0.002981936347127975 72-73 5.352193443563032E-4 3.822995316830737E-5 0.0 0.7982223071776737 0.0032304310427219724 74-75 5.352193443563032E-4 3.822995316830737E-5 0.0 0.8897257000860175 0.0032877759724744336 76-77 5.352193443563032E-4 3.822995316830737E-5 0.0 0.992296664436586 0.0032877759724744336 78-79 5.352193443563032E-4 3.822995316830737E-5 0.0 1.1168307368823474 0.003459810761731817 80-81 5.352193443563032E-4 3.822995316830737E-5 0.0 1.2525853005830068 0.003517155691484278 82-83 5.734492975246105E-4 3.822995316830737E-5 0.0 1.3982414221542578 0.0037465354104941223 84-85 5.734492975246105E-4 3.822995316830737E-5 0.0 1.5523845933288731 0.0037465354104941223 86-87 5.734492975246105E-4 3.822995316830737E-5 0.0 1.7110388989773488 0.003861225269999044 88-89 5.734492975246105E-4 3.822995316830737E-5 0.0 1.8893242855777501 0.004014145082672274 90-91 5.734492975246105E-4 3.822995316830737E-5 0.0 2.0980598298767084 0.004014145082672274 92-93 5.734492975246105E-4 3.822995316830737E-5 0.0 2.3220682404664057 0.0042435248016821185 94-95 5.734492975246105E-4 3.822995316830737E-5 0.0 2.5501672560451114 0.004319984708018733 96-97 5.734492975246105E-4 3.822995316830737E-5 0.0 2.7921246296473288 0.004453789544107809 98-99 5.734492975246105E-4 3.822995316830737E-5 0.0 3.055032017585779 0.004549364427028577 100-101 5.734492975246105E-4 3.822995316830737E-5 0.0 3.357622096912931 0.004549364427028577 102-103 5.734492975246105E-4 3.822995316830737E-5 0.0 3.681506260154831 0.004702284239701806 104-105 5.734492975246105E-4 3.822995316830737E-5 0.0 4.016553569721877 0.00472139921628596 106-107 5.734492975246105E-4 3.822995316830737E-5 0.0 4.351772914078181 0.0047405141928701135 108-109 5.734492975246105E-4 3.822995316830737E-5 0.0 4.709070056389181 0.004816974099206728 110-111 5.734492975246105E-4 3.822995316830737E-5 0.0 5.107062983847845 0.005008123865048265 112-113 5.734492975246105E-4 3.822995316830737E-5 0.0 5.544470993023033 0.005046353818216573 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3455 0.0 51.83801 1 GTATCAA 6465 0.0 49.97596 1 ATCAACG 7695 0.0 41.368214 3 CAACGCA 7750 0.0 40.921078 5 TCAACGC 7770 0.0 40.815746 4 TATCAAC 8060 0.0 39.421017 2 AACGCAG 8140 0.0 39.026123 6 ACGCAGA 9195 0.0 34.16023 7 CGCAGAG 9230 0.0 33.261715 8 GTACATG 10640 0.0 31.31672 1 TACATGG 10675 0.0 30.04294 2 ACATGGG 10345 0.0 29.908484 3 TAAGGTG 1660 0.0 27.59969 5 GCAGAGT 10830 0.0 27.194538 9 AGAGTAC 9120 0.0 25.546122 10-11 GTAAGGT 1605 0.0 24.096828 4 CATGGGG 5930 0.0 23.579508 4 CAGAGTA 10065 0.0 22.763285 10-11 CATGGGA 4905 0.0 22.684238 4 AGGTAAG 1535 0.0 22.482323 2 >>END_MODULE