FastQCFastQC Report
Thu 26 May 2016
SRR1364556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615750
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59130.22605371308420147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50110.19157029532638822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.11430755997323903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.058.4425741
GTATCAA87350.050.720411
ATCAACG109600.039.8375783
TCAACGC109250.039.6380654
TATCAAC111800.038.947092
CAACGCA110950.038.933075
AACGCAG115650.037.505186
ACGCAGA136300.031.5610757
CGCAGAG136200.031.4531948
GTACATG104900.029.8428761
TACATGG107200.028.7828962
ACATGGG104200.028.0109483
GCAGAGT152050.027.1168699
GAGTACT90200.025.39492612-13
AGAGTAC132000.024.47336810-11
GTACTTT93800.024.16656314-15
CAGAGTA143350.023.75995610-11
CATGGGA52800.023.5782664
TAAGGTG16250.022.7011415
AGTACTT99950.022.32241812-13
Produced by FastQC (version 0.11.5)